Enzymatic Activity of Coagulase-positive Staphylococcus aureus Strains Isolated from Patients and Healthy Carriers

Pathobiology ◽  
1966 ◽  
Vol 29 (3) ◽  
pp. 307-323
Author(s):  
W. Kedzia ◽  
M. Musielak ◽  
B. Kedzia ◽  
H. Koniar ◽  
E. Pniewska
Keyword(s):  
Staphylococcus Aureus ◽  
Enzymatic Activity ◽  
Healthy Carriers

Staphylococcus aureus Types Isolated from Hospital Sources and from Healthy Carriers

Pathobiology ◽  
1966 ◽  
Vol 29 (6) ◽  
pp. 854-864
Author(s):  
S. Krynski ◽  
E. Becla ◽  
R. Bugalski ◽  
Anna Podhajska ◽  
A. Samet
Keyword(s):  
Staphylococcus Aureus ◽  
Healthy Carriers

scholarly journals Infection by Staphylococcus aureus in healthy carrier children residing in Pachuca, Hidalgo State, Mexico

2012 ◽  
Vol 1 (1) ◽  
Author(s):  
Marco Antonio Becerril-Flores ◽  
César Arturo Gómez Durán ◽  
José Anselmo Hernández Ibarra ◽  
Erika Janet Varela-Prado ◽  
Areli Cruz Castañeda ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Public Health Problem ◽  
Antibiotics Resistance ◽  
Healthy Children ◽  
Gram Staining ◽  
Asymptomatic Children ◽  
Significant Difference ◽  
Resistant Strains ◽  
Epidemiologic Risk ◽  
Healthy Carriers

Infections by Staphylococcus aureus are a great worldwide public health problem due to easiness of transmission, via inhalation or direct contact in hospitals. Among infected individuals there are asymptomatic carriers that cause propagation and transmission of these infections within the hospital population. In Mexico, studies about prevalence of infections in healthy carriers by this bacterium are scarce. We investigated the prevalence of pharyngeal infections by S. aureus in asymptomatic children (3 to 8 years old) resident in Pachuca, Hidalgo. There were previous old clinic inspections as part of the study wherein 138 healthy children were studied. Samples of exudates from children were obtained and seeded in selective and differential media and they were morphologically identified as S. aureus by Gram staining. Antibiotics resistance was determined for each sample of bacteria. Prevalence of S. aureus infection was 20.29%, 12.69% in girls, and 26.66% in boys. Pre-school children showed a higher rate of infection than those in elementary school but there was not a significant difference between them (Χ2=0.92, p>0.05). More than 40% of S. aureus strains were ß-hemolytic, and half of the bacteria isolated from children in preschool is resistant to any of the antibiotics studied and resistant to at least one of the antibiotics assayed. The greatest resistance was to ciprofloxacin and erythromycin. Finding children infected with S. aureus as healthy carriers may be, in fact an epidemiologic risk for the entire population. Furthermore, since there are resistant strains of this microorganism, physicians must be very sensible of resistant patterns when selecting antibiotics.

scholarly journals Comparison of protein — A with clumping factor, hemolysis and coagulase tests for identification of Staphylococci isolated from Nasal swabs of healthy carriers

2007 ◽  
Vol 4 (1) ◽  
pp. 83-88
Author(s):  
Baghdad Science Journal
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Blood Agar ◽  
Healthy Human ◽  
Nasal Swabs ◽  
Healthy Carriers

Thirty nine (12.8%) isolates of Staphylococcus aureus were isolated from 304 healthy human (Nasal swabs). It was found that percentage of males that have S. aureus is more than female's percentage. These isolates (39) were tested with different tests. Twenty seven isolates (69.23 %) were positive for Staphylococcus protein —A (SPA) ,thirty seven ( 94.8 %) were positive for tube coagulase , thirty five ( 89.7 % ) were positive with clumping factor and thirty two ( 82.05 %) had 13 — hemolytic on blood agar. It was found that 100% of the isolates (39 isolates) were positive with one, two or three tests (tube coagulase, clumping factor and SPA).

scholarly journals Sortase mutants with improved protein thermostability and enzymatic activity obtained by consensus design

2019 ◽  
Vol 32 (12) ◽  
pp. 555-564
Author(s):  
Magdalena Wójcik ◽  
Susana Vázquez Torres ◽  
Wim J Quax ◽  
Ykelien L Boersma
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Enzymatic Activity ◽  
Sortase A ◽  
Wild Type ◽  
Protein Thermostability ◽  
Consensus Analysis ◽  
Gram Positive Bacteria ◽  
Covalently Linked

Abstract Staphylococcus aureus sortase A (SaSrtA) is an enzyme that anchors proteins to the cell surface of Gram-positive bacteria. During the transpeptidation reaction performed by SaSrtA, proteins containing an N-terminal glycine can be covalently linked to another protein with a C-terminal LPXTG motif (X being any amino acid). Since the sortase reaction can be performed in vitro as well, it has found many applications in biotechnology. Although sortase-mediated ligation has many advantages, SaSrtA is limited by its low enzymatic activity and dependence on Ca2+. In our study, we evaluated the thermodynamic stability of the SaSrtA wild type and found the enzyme to be stable. We applied consensus analysis to further improve the enzyme’s stability while at the same time enhancing the enzyme’s activity. As a result, we found thermodynamically improved, more active and Ca2+-independent mutants. We envision that these new variants can be applied in conjugation reactions in low Ca2+ environments.

scholarly journals Detection of endogenous MazF enzymatic activity in Staphylococcus aureus

2013 ◽  
Vol 443 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Julia J. van Rensburg ◽  
Paul J. Hergenrother
Keyword(s):  
Staphylococcus Aureus ◽  
Enzymatic Activity

scholarly journals The Staphylococcus aureus Autoinducer-2 Synthase LuxS Is Regulated by Ser/Thr Phosphorylation

2010 ◽  
Vol 192 (23) ◽  
pp. 6295-6301 ◽  
Author(s):  
Marie-Eve Cluzel ◽  
Isabelle Zanella-Cléon ◽  
Alain J. Cozzone ◽  
Klaus Fütterer ◽  
Bertrand Duclos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Enzymatic Activity ◽  
Specific System ◽  
Unique Position ◽  
Autoinducer 2

ABSTRACT The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing enzyme regulated by phosphorylation and demonstrating that S. aureus possesses an original and specific system for controlling AI-2 synthesis.

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