Superoxide Dismutase Activity and Reduced Glutathione Content in Erythrocytes of Uremic Patients on Chronic Dialysis

1983 ◽  
Vol 70 (5) ◽  
pp. 312-315 ◽  
Author(s):  
A. Vanella ◽  
E. Geremia ◽  
R. Pinturo ◽  
P. Tiriolo ◽  
G. Liuzzo ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Chronic Dialysis ◽  
Uremic Patients

scholarly journals Effect of catalase inactivation on levels of inorganic peroxides, superoxide dismutase, glutathione, oxygen consumption and life span in adult houseflies (Musca domestica)

1983 ◽  
Vol 216 (2) ◽  
pp. 503-506 ◽  
Author(s):  
R G Allen ◽  
K J Farmer ◽  
R S Sohal
Keyword(s):  
Superoxide Dismutase ◽  
Oxygen Consumption ◽  
Life Span ◽  
Musca Domestica ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Adaptive Responses ◽  
Total Inhibition ◽  
Rate Of Oxygen Consumption

The effects of total inhibition of catalase, induced by 3-amino-1,2,4-triazole, on the adult housefly (Musca domestica) were examined. The lack of catalase activity had no effect on the longevity of the houseflies. Inorganic-peroxide concentration was elevated at younger ages, but declined in older flies. The rate of oxygen consumption by the flies was greatly decreased and the levels of oxidized as well as reduced glutathione were augmented. Superoxide dismutase activity showed a slight increase. This study suggests that loss of catalase activity does not affect survival of houseflies due to adaptive responses.

scholarly journals Melatonin and garlic cytoprotective-ameliorative effects on dibutyl phthalate intoxication on sperm DNA and testicular biomakers of rabbits

2020 ◽  
Vol 18 (3) ◽  
pp. 158-166
Author(s):  
M Shinkut ◽  
T Aluwong ◽  
P.I. Rekwot ◽  
A.I. Nwannenna ◽  
F.U. Samuel
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Dibutyl Phthalate ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Activity Increase ◽  
Sperm Dna ◽  
Group B

The study investigated the cytoprotective and ameliorative effects of melatonin and Allium sativum (garlic) on dibutyl phthalate (DBP)-induced oxidative stress, its impact on sperm DNA integrity and testicular oxidative stress biomarkers. Forty two rabbit bucks were randomly divided into 7 groups of 6 bucks each labeled as A, B, C, D, E, F and G: The treatment were as follows: A (served as negative control, received olive oil for 16 weeks); B (served as positive control, exposed to DBP for 16 weeks, no treatment); C (given melatonin for 8 weeks, thereafter DBP for 8 weeks); D (administered garlic for 8 weeks, thereafter DBP for 8 weeks); E (exposed to DBP for 8 weeks, thereafter melatonin for 8 week); F (exposed to DBP for 8 weeks, thereafter garlic for 8 weeks); and G (exposed to DBP for 8 weeks, thereafter melatonin + garlic for 8 weeks). Ejaculated semen was collected on the last day (112th) using artificialv vagina for rabbit and pooled for each group was used for sperm DNA fragmentation index (SDFI) determination, rabbits were sacrificed and the testes harvested for determination of superoxide dismutase activity, reduced glutathione and malondialdehyde concentration. Results showed a significant increase (P = 0.0018) in the mean SDFI in group B (78.20 ± 4.72), compared to other groups. A significant increase (P ≤ 0.0001) in superoxide dismutase activity, increase reduced glutathione concentration and decrease malondialdehyde concentrations in the treatment groups compared to the DBP exposed group without treatment (group B) were observed. Melatonin and garlic demonstrated cytoprotective and ameliorative effects against DBP-induced oxidative stress in rabbit bucks. Keywords: Dibutyl phthalate, Garlic, Melatonin, Sperm DNA, Testicular biomarkers

SUPEROXIDE DISMUTASE ACTIVITY AND REDUCED GLUTATHIONE LEVELS IN Cu-OVERLOAD LIVER FROM SHEEP

1997 ◽  
Vol 17 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Aldo S Sansinanea ◽  
Silvia I Cerone ◽  
Sergio A Streitenberger ◽  
Cecilia García ◽  
Nestor Auza
Keyword(s):  
Superoxide Dismutase ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity

scholarly journals State of antioxidant system of rat blood plasma at the action of quartzetin and histamine in in vitro experiments

2021 ◽  
pp. 40-53
Author(s):  
N. Harasym ◽  
◽  
H. Baran ◽  
N. Bodnarchuk ◽  
V. Otchych ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Antioxidant System ◽  
Biogenic Amine ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Simultaneous Action ◽  
Minimum Concentration ◽  
Combined Action

The effect of histamine and quercetin, as well as their combined effect on the activity of superoxide dismutase, catalase and the content of reduced glutathione in the blood plasma of rats was studied. It was found that the addition to the blood of quercetin at a concentration of 0.1; 0.3; 0.5; 1.0; 5.0 mM causes an increase in superoxide dismutase activity. It was found that histamine at concentrations of .01 and 0.1 μm leads to a decrease in superoxide dismutase activity by 31 and 17 %, respectively. Whereas the biogenic amine in the lowest and highest concentrations does not change the activity of superoxide dismutase in plasma. At simultaneous introduction into blood of histamine in the maximum concentration (10.0 μm) and quercetin in concentration of 0,1; 0.5; 3.0 mM normalizes the activity of superoxide dismutase. And only the combined action of histamine of this concentration and quercetin at a concentration of 5.0 mM reduces the activity of the enzyme by 21 %. Histamine at a concentration of 0.01 μm and the simultaneous action of quercetin at a concentration of 0.1; 0.5; 3.0; 5.0 mM increases the activity of superoxide dismutase, which indicates the generation of reactive oxygen species, in particular the superoxide anion radical. It was found that the addition of whole concentrations of quercetin to whole blood causes a decrease in plasma catalase activity. The combined action of quercetin and histamine causes a decrease in catalase activity. It was found that the addition to the blood of quercetin at a concentration of 0.1; 0.3; 0.5; 1.0 mM causes a decrease in the content of reduced glutathione. Quercetin at a concentration of 3.0 and 5.0 mM causes an increase in reduced glutathione by 27 and 14 %, respectively, compared to the reference plasma. Histamine at concentrations of 10.0, 1.0 and 0.01 μm leads to an increase in the amount of reduced glutathione by 24, 26 and 19 %, respectively. And at a concentration of 0.1 μm, the biogenic amine reduces the GSH content by 39 %. With simultaneous introduction into the blood of histamine at a concentration of 10.0 μm and quercetin at a concentration of 0.1; 0.5; 3.0 mM there is an increase in the content of reduced glutathione. And only at a concentration of 5.0 mM quercetin on the background of the action of histamine (10.0 μm), the content of reduced glutathione is slightly reduced. However, with the combined action of histamine at a minimum concentration (0.01 μM) and quercetin at a concentration of 0.1; 0.5; 3.0 and 5.0 mM there is a decrease in the content of reduced glutathione. After performing a dispersion analysis, it was found that the state of the antioxidant system, both enzymatic and non-enzymatic, is significantly affected by histamine. The smallest, but significant share of the effect is on the action of quercetin in the blood plasma of rats. The indirect and significant effect of the simultaneous action of histamine and quercetin on the antioxidant system of rat plasma was revealed.

Effect of Fenugreek Seeds and Probiotic Supplementation on Antioxidant Status and Milk Production in Heat Stressed Beetal Goats

2020 ◽  
Author(s):  
Raj Sahoo ◽  
Shashi Nayyar ◽  
Chanchal Singh ◽  
Sandeep Kaswan ◽  
Sandeep Sodhi Kakkar ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Reduced Glutathione ◽  
Group Iv ◽  
Superoxide Dismutase Activity ◽  
Yeast Cells ◽  
Probiotic Supplementation ◽  
Fenugreek Seeds ◽  
Group Iii ◽  
Group I ◽  
Total Average

Twenty lactating Beetal goats were divided as Pre-summer group: (n=5) without supplementation and Summer group: (n=20). The latter group was further divided in sub-groups namely Group I: (n=5) Control, no supplementation; Group II: (n=5) supplemented with fenugreek seeds @ 10g/animal/day for 30 days; Group III: (n=5) supplemented with Yea Sacc®1026 @ 2g consisting of 10 billion yeast cells/animal/day for 30 days and Group IV: (n=5) supplemented with fenugreek seeds and Yea Sacc®1026 combination for 30 days. Summer stressed goats exhibited elevated levels of malondialdehyde and reduced the levels of Superoxide dismutase activity, Vitamin E, Vitamin C and reduced glutathione. Supplementation of fenugreek seeds and Yea Sacc®1026 reduced levels of lipid peroxidation and improved the superoxide dismutase activity. Total average milk yield (kg/day) was the highest in group IV (0.988±0.03) followed by groups II (0.889±0.02) and III (0.785±0.012) which was significantly higher as compared to the unsupplemented group I (0.618±0.004).

Diazoxide Modulates Cardiac Hypertrophy by Targeting H2O2 Generation and Mitochondrial Superoxide Dismutase Activity

2020 ◽  
Vol 13 (1) ◽  
pp. 76-83
Author(s):  
Aline Maria Brito Lucas ◽  
Joana Varlla de Lacerda Alexandre ◽  
Maria Thalyne Silva Araújo ◽  
Cicera Edna Barbosa David ◽  
Yuana Ivia Ponte Viana ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Cardiac Hypertrophy ◽  
Superoxide Dismutase Activity ◽  
Heart Weight ◽  
Pharmacological Intervention ◽  
Wall Thickening ◽  
H2o2 Production ◽  
Mitochondrial Superoxide ◽  
Mitochondrial Superoxide Dismutase

Background: Cardiac hypertrophy involves marked wall thickening or chamber enlargement. If sustained, this condition will lead to dysfunctional mitochondria and oxidative stress. Mitochondria have ATP-sensitive K+ channels (mitoKATP) in the inner membrane that modulate the redox status of the cell. Objective: We investigated the in vivo effects of mitoKATP opening on oxidative stress in isoproterenol- induced cardiac hypertrophy. Methods: Cardiac hypertrophy was induced in Swiss mice treated intraperitoneally with isoproterenol (ISO - 30 mg/kg/day) for 8 days. From day 4, diazoxide (DZX - 5 mg/kg/day) was used in order to open mitoKATP (a clinically relevant therapy scheme) and 5-hydroxydecanoate (5HD - 5 mg/kg/day) or glibenclamide (GLI - 3 mg/kg/day) were used as mitoKATP blockers. Results: Isoproterenol-treated mice had elevated heart weight/tibia length ratios (HW/TL). Additionally, hypertrophic hearts had elevated levels of carbonylated proteins and Thiobarbituric Acid Reactive Substances (TBARS), markers of protein and lipid oxidation. In contrast, mitoKATP opening with DZX avoided ISO effects on gross hypertrophic markers (HW/TL), carbonylated proteins and TBARS, in a manner reversed by 5HD and GLI. Moreover, DZX improved mitochondrial superoxide dismutase activity. This effect was also blocked by 5HD and GLI. Additionally, ex vivo treatment of isoproterenol- induced hypertrophic cardiac tissue with DZX decreased H2O2 production in a manner sensitive to 5HD, indicating that this drug also acutely avoids oxidative stress. Conclusion: Our results suggest that diazoxide blocks oxidative stress and reverses cardiac hypertrophy. This pharmacological intervention could be a potential therapeutic strategy to prevent oxidative stress associated with cardiac hypertrophy.

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