Age-associated changes in superoxide dismutase activity, thiobarbituric acid reactivity and reduced glutathione level in the brain and liver in senescence accelerated mice (SAM): a comparison with ddY mice

1993 ◽  
Vol 71 (1-2) ◽  
pp. 23-30 ◽  
Author(s):  
J LIU ◽  
A MORI
Keyword(s):  
Superoxide Dismutase ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Superoxide Dismutase Activity ◽  
Glutathione Level ◽  
The Brain ◽  
Senescence Accelerated Mice

scholarly journals Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues

2012 ◽  
Vol 84 (4) ◽  
pp. 1121-1126 ◽  
Author(s):  
Seyed M. Nabavi ◽  
Seyed F. Nabavi ◽  
Akbar H. Moghaddam ◽  
William N. Setzer ◽  
Morteza Mirzaei
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Sodium Fluoride ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Glutathione Level ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cardiac Tissues ◽  
And Oxidative Stress

This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals before sodium fluoride consumption modified the levels of biochemical parameters.

scholarly journals Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

2015 ◽  
Vol 62 (2) ◽  
pp. 13-19
Author(s):  
Urmila Jarouliya ◽  
Anish Zacharia ◽  
Raj K. Keservani ◽  
Godavarthi B.K.S Prasad
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

scholarly journals Effect of catalase inactivation on levels of inorganic peroxides, superoxide dismutase, glutathione, oxygen consumption and life span in adult houseflies (Musca domestica)

1983 ◽  
Vol 216 (2) ◽  
pp. 503-506 ◽  
Author(s):  
R G Allen ◽  
K J Farmer ◽  
R S Sohal
Keyword(s):  
Superoxide Dismutase ◽  
Oxygen Consumption ◽  
Life Span ◽  
Musca Domestica ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Adaptive Responses ◽  
Total Inhibition ◽  
Rate Of Oxygen Consumption

The effects of total inhibition of catalase, induced by 3-amino-1,2,4-triazole, on the adult housefly (Musca domestica) were examined. The lack of catalase activity had no effect on the longevity of the houseflies. Inorganic-peroxide concentration was elevated at younger ages, but declined in older flies. The rate of oxygen consumption by the flies was greatly decreased and the levels of oxidized as well as reduced glutathione were augmented. Superoxide dismutase activity showed a slight increase. This study suggests that loss of catalase activity does not affect survival of houseflies due to adaptive responses.

Superoxide dismutase activity in the brain of rats prenatally treated with lead

1999 ◽  
Vol 128 (6) ◽  
pp. 1239-1240
Author(s):  
M. G. Uzbekov ◽  
I. K. Karpachevskaya ◽  
N. I. Bubnova ◽  
G. V. Kulikova
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Dismutase Activity ◽  
The Brain

The Influence of Supplementation with Rhodiola rosea L. Extract on Selected Redox Parameters in Professional Rowers

2009 ◽  
Vol 19 (2) ◽  
pp. 186-199 ◽  
Author(s):  
Anna Skarpanska-Stejnborn ◽  
Lucja Pilaczynska-Szczesniak ◽  
Piotr Basta ◽  
Ewa Deskur-Smielecka
Keyword(s):  
Superoxide Dismutase ◽  
Placebo Group ◽  
Antioxidant Capacity ◽  
Thiobarbituric Acid ◽  
Creatine Kinase Activity ◽  
Superoxide Dismutase Activity ◽  
Rhodiola Rosea ◽  
Blood Samples ◽  
Plasma Antioxidant Capacity ◽  
Lactate Levels

The aim of this study was to investigate the effect of Rhodiola rosea supplementation on the balance of oxidants and antioxidants in the serum and erythrocytes of competitive rowers. This double-blinded study included 22 members of the Polish Rowing Team who were participating in a preparatory camp. Participants were randomly assigned to the supplemented group (n = 11), who received 100 mg of R. rosea extract twice daily for 4 wk, or the placebo group (n = 11). At the beginning and end of the study, participants performed a 2,000-m maximum test on a rowing ergometer. Blood samples were taken from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-hr restitution period. The following redox parameters were assessed in erythrocytes: superoxide dismutase activity, glutathione peroxidase activity, and thiobarbituric-acid-reactive substances concentrations. In addition, creatine kinase activity and total antioxidant capacity were measured in plasma samples, lactate levels were determined in capillary blood samples, and uric acid concentrations were measured in serum. After supplementation, the total plasma antioxidant capacity was significantly higher (p = .0002) in the supplemented group than in the placebo group, and superoxide dismutase activity in erythrocytes directly after and 24 hr after the ergometry was significantly (p = .0461) lower in athletes receiving R. rosea extracts than in the placebo group. In conclusion, supplementation with R. rosea increased antioxidant levels in the plasma of professional rowers but had no effect on oxidative damage induced by exhaustive exercise.

Superoxide Dismutase Activity and Reduced Glutathione Content in Erythrocytes of Uremic Patients on Chronic Dialysis

1983 ◽  
Vol 70 (5) ◽  
pp. 312-315 ◽  
Author(s):  
A. Vanella ◽  
E. Geremia ◽  
R. Pinturo ◽  
P. Tiriolo ◽  
G. Liuzzo ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Chronic Dialysis ◽  
Uremic Patients

scholarly journals Melatonin and garlic cytoprotective-ameliorative effects on dibutyl phthalate intoxication on sperm DNA and testicular biomakers of rabbits

2020 ◽  
Vol 18 (3) ◽  
pp. 158-166
Author(s):  
M Shinkut ◽  
T Aluwong ◽  
P.I. Rekwot ◽  
A.I. Nwannenna ◽  
F.U. Samuel
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Dibutyl Phthalate ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Activity Increase ◽  
Sperm Dna ◽  
Group B

The study investigated the cytoprotective and ameliorative effects of melatonin and Allium sativum (garlic) on dibutyl phthalate (DBP)-induced oxidative stress, its impact on sperm DNA integrity and testicular oxidative stress biomarkers. Forty two rabbit bucks were randomly divided into 7 groups of 6 bucks each labeled as A, B, C, D, E, F and G: The treatment were as follows: A (served as negative control, received olive oil for 16 weeks); B (served as positive control, exposed to DBP for 16 weeks, no treatment); C (given melatonin for 8 weeks, thereafter DBP for 8 weeks); D (administered garlic for 8 weeks, thereafter DBP for 8 weeks); E (exposed to DBP for 8 weeks, thereafter melatonin for 8 week); F (exposed to DBP for 8 weeks, thereafter garlic for 8 weeks); and G (exposed to DBP for 8 weeks, thereafter melatonin + garlic for 8 weeks). Ejaculated semen was collected on the last day (112th) using artificialv vagina for rabbit and pooled for each group was used for sperm DNA fragmentation index (SDFI) determination, rabbits were sacrificed and the testes harvested for determination of superoxide dismutase activity, reduced glutathione and malondialdehyde concentration. Results showed a significant increase (P = 0.0018) in the mean SDFI in group B (78.20 ± 4.72), compared to other groups. A significant increase (P ≤ 0.0001) in superoxide dismutase activity, increase reduced glutathione concentration and decrease malondialdehyde concentrations in the treatment groups compared to the DBP exposed group without treatment (group B) were observed. Melatonin and garlic demonstrated cytoprotective and ameliorative effects against DBP-induced oxidative stress in rabbit bucks. Keywords: Dibutyl phthalate, Garlic, Melatonin, Sperm DNA, Testicular biomarkers

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