Effect of Age and Short-Term Cold Stress on SDH Activity and Oxygen Consumption of Lizard Brain

We have suggested previously that Tribbles homolog 3 (TRIB3), a negative regulator of Akt activity in insulin-sensitive tissues, could mediate glucose-induced insulin resistance in muscle under conditions of chronic hyperglycemia (Liu J, Wu X, Franklin JL, Messina JL, Hill HS, Moellering DR, Walton RG, Martin M, Garvey WT. Am J Physiol Endocrinol Metab 298: E565–E576, 2010). In the current study, we have assessed short-term physiological regulation of TRIB3 in skeletal muscle and adipose tissues by nutrient excess and fasting as well as TRIB3's ability to modulate glucose transport and mitochondrial oxidation. In Sprague-Dawley rats, we found that short-term fasting enhanced insulin sensitivity concomitantly with decrements in TRIB3 mRNA (66%, P < 0.05) and protein (81%, P < 0.05) in muscle and increments in TRIB3 mRNA (96%, P < 0.05) and protein (∼10-fold, P < 0.05) in adipose tissue compared with nonfasted controls. On the other hand, rats fed a Western diet for 7 days became insulin resistant concomitantly with increments in TRIB3 mRNA (155%, P < 0.05) and protein (69%, P = 0.0567) in muscle and a decrease in the mRNA (76%, P < 0.05) and protein (70%, P < 0.05) in adipose. In glucose transport and mitochondria oxidation studies using skeletal muscle cells, we found that stable TRIB3 overexpression impaired insulin-stimulated glucose uptake without affecting basal glucose transport and increased both basal glucose oxidation and the maximal uncoupled oxygen consumption rate. With stable knockdown of TRIB3, basal and insulin-stimulated glucose transport rates were increased, whereas basal glucose oxidation and the maximal uncoupled oxygen consumption rate were decreased. In conclusion, TRIB3 impacts glucose uptake and oxidation oppositely in muscle and fat according to levels of nutrient availability. The above data for the first time implicate TRIB3 as a potent physiological regulator of insulin sensitivity and mitochondrial glucose oxidation under conditions of nutrient deprivation and excess.

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The effect of age on short-term postoperative complications following arthroscopic rotator cuff repair

JSES Open Access ◽

10.1016/j.jses.2019.07.010 ◽

2019 ◽

Vol 3 (3) ◽

pp. 194-198 ◽

Cited By ~ 4

Author(s):

Ajay S. Padaki ◽

Venkat Boddapati ◽

Justin Mathew ◽

Christopher S. Ahmad ◽

Charles M. Jobin ◽

...

Keyword(s):

Postoperative Complications ◽

Rotator Cuff ◽

Rotator Cuff Repair ◽

Arthroscopic Rotator Cuff Repair ◽

Short Term ◽

Arthroscopic Rotator Cuff ◽

Cuff Repair ◽

Effect Of Age

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Postprandial gastrointestinal blood flow and oxygen consumption during environmental cold stress

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1989.256.2.g364 ◽

1989 ◽

Vol 256 (2) ◽

pp. G364-G368

Author(s):

S. R. Mayfield ◽

W. Oh ◽

D. L. Piva ◽

B. S. Stonestreet

Keyword(s):

Blood Flow ◽

Oxygen Consumption ◽

Cold Stress ◽

Cold Environment ◽

Experimental Phase ◽

Phase Measurements ◽

Control Phase ◽

Warm Environment ◽

Environment Control ◽

Gastrointestinal Blood Flow

We studied the independent and combined effects of feeding and environmental cold stress by measuring pre- and postprandial gastrointestinal blood flow (QGI), oxygen consumption (GIVO2), and oxygen extraction (GIO2ex) in seven awake 3- to 4-day-old piglets while in a thermoneutral environment (control phase, 31 degrees C) and during environmental cold stress (experimental phase, 20.5 degrees C). Each animal consecutively completed both the control and experimental phases. In the control phase, measurements were made before and 30 min after feeding. In the experimental phase, measurements were made before and 30 min after induction of cold stress. A feeding was then given and measurements repeated 30 min later during continued cold stress. QGI (ml.100 g-1.min-1) increased postprandially while in a thermoneutral environment (130 +/- 11 to 152 +/- 12) but not while in a cold environment (126 +/- 15 to 121 +/- 8). Postprandial GIVO2 (ml O2.100 g-1.min-1) increased from 2.1 +/- 0.2 to 3.7 +/- 0.5 while in a warm environment. During preprandial cold stress, an unexpected increase in GIVO2 was observed (1.7 +/- 0.2 to 3.1 +/- 0.5). Feeding while in a cold environment provoked a further significant increase in GIVO2 (3.1 +/- 0.5 to 4.2 +/- 0.4). All increases in GIVO2 were associated with increased GIO2ex. This study has demonstrated that 1) postprandial GIVO2 is appropriately increased during cold stress as a function of GIO2ex and 2) that fasting GIVO2 is increased during cold stress, demonstrating an intestinal metabolic contribution to cold-induced systemic thermogenesis.

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Short-term repeatability of oxygen consumption-heart rate relationships

American Journal of Clinical Nutrition ◽

10.1093/ajcn/32.9.1758 ◽

1979 ◽

Vol 32 (9) ◽

pp. 1758-1759 ◽

Cited By ~ 2

Author(s):

R B Bradfield

Keyword(s):

Heart Rate ◽

Oxygen Consumption ◽

Short Term

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Effect of short-term cold stress on oxidative damage and transcript accumulation of defense-related genes in chickpea seedlings

Journal of Plant Physiology ◽

10.1016/j.jplph.2014.03.020 ◽

2014 ◽

Vol 171 (13) ◽

pp. 1106-1116 ◽

Cited By ~ 25

Author(s):

Seyyedeh-Sanam Kazemi-Shahandashti ◽

Reza Maali-Amiri ◽

Hassan Zeinali ◽

Mona Khazaei ◽

Alireza Talei ◽

...

Keyword(s):

Oxidative Damage ◽

Cold Stress ◽

Short Term ◽

Transcript Accumulation

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Effect of Age on the Oxygen Consumption and Glucose Uptake by the Elastic Cartilage of Rat

Gerontology ◽

10.1159/000211601 ◽

1967 ◽

Vol 13 (3) ◽

pp. 173-176 ◽

Cited By ~ 2

Author(s):

B.K. Patnaik

Keyword(s):

Oxygen Consumption ◽

Glucose Uptake ◽

Elastic Cartilage ◽

Effect Of Age

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Dynamic modelling of cold-hardiness in tea buds by imitating past temperature memory

Annals of Botany ◽

10.1093/aob/mcaa197 ◽

2020 ◽

Author(s):

Kensuke Kimura ◽

Daisuke Yasutake ◽

Takahiro Oki ◽

Koichiro Yoshida ◽

Masaharu Kitano

Keyword(s):

Cold Stress ◽

Cold Acclimation ◽

Dynamic Model ◽

Cold Hardiness ◽

Global Climate ◽

Effective Means ◽

Dynamic Modelling ◽

Short Term ◽

Mean Square Errors

Abstract Background and Aims Most perennial plants memorize cold stress for a certain period and retrieve the memories for cold acclimation and deacclimation, which leads to seasonal changes in cold-hardiness. Therefore, a model for evaluating cold stress memories is required for predicting cold-hardiness and for future frost risk assessments under warming climates. In this study we develop a new dynamic model of cold-hardiness by introducing a function imitating past temperature memory in the processes of cold acclimation and deacclimation. Methods We formulated the past temperature memory for plants using thermal time weighted by a forgetting function, and thereby proposed a dynamic model of cold-hardiness. We used the buds of tea plants (Camellia sinensis) from two cultivars, ‘Yabukita’ and ‘Yutakamidori’, to calibrate and validate this model based on 10 years of observed cold-hardiness data. Key Results The model captured more than 90 % of the observed variation in cold-hardiness and predicted accurate values for both cultivars, with root mean square errors of ~1.0 °C. The optimized forgetting function indicated that the tea buds memorized both short-term (recent days) and long-term (previous months) temperatures. The memories can drive short-term processes such as increasing/decreasing the content of carbohydrates, proteins and antioxidants in the buds, as well as long-term processes such as determining the bud phenological stage, both of which vary with cold-hardiness. Conclusions The use of a forgetting function is an effective means of understanding temperature memories in plants and will aid in developing reliable predictions of cold-hardiness for various plant species under global climate warming.

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