scholarly journals Clear Correlation of Tetrahydrobiopterin with Nitric Oxide Bioavailability in Continuous Ambulatory Peritoneal Dialysis

2010 ◽  
Vol 30 (2) ◽  
pp. 96-97
Author(s):  
Seiichi Mochizuki ◽  
Aya Takayama ◽  
Tamaki Sasaki ◽  
Toyotaka Yada ◽  
Kousuke Endo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Clear Correlation ◽  
Nitric Oxide Bioavailability

Increased excretion of nitric oxide in exhaled air of patients with chronic renal failure

1999 ◽  
Vol 96 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Akihiro MATSUMOTO ◽  
Yasunobu HIRATA ◽  
Masao KAKOKI ◽  
Daisuke NAGATA ◽  
Shin-ichi MOMOMURA ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Renal Failure ◽  
Peritoneal Dialysis ◽  
Chronic Renal Failure ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Exhaled Nitric Oxide ◽  
Simultaneous Increase ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Endogenous Nitric Oxide

Nitric oxide exerts multiple effects on renal function. It remains unclear whether endogenous nitric oxide production is increased or decreased in patients with chronic renal failure. To evaluate endogenous nitric oxide production in these patients we studied exhaled nitric oxide output by an ozone chemiluminescence method and plasma NO2-/NO3- levels by the Griess method in 40 patients with end-stage chronic renal failure who underwent regular continuous ambulatory peritoneal dialysis (n = 30) or haemodialysis (n = 10), and in 28 healthy subjects. Patients with chronic renal failure had a higher exhaled nitric oxide concentration [39±3 versus 19±1 parts per billion, (mean±S.E.M.), P< 0.0001], a greater nitric oxide output (177±11 versus 96±7 ;nl·min-1·m-2, P< 0.001) and a higher plasma NO2-/NO3- concentration (96±14 versus 33±4 ;μmol, P< 0.01) than controls. These values did not differ between patients on haemodialysis and those on continuous ambulatory peritoneal dialysis. Patients with chronic renal failure had significantly higher plasma concentrations of both interleukin-1β and interferon-γ than controls. The exhaled nitric oxide output did not correlate with plasma NO2-/NO3- or with peritoneal dialysate NO2-/NO3-, but plasma NO2-/NO3- correlated with dialysate NO2-/NO3- in patients who underwent continuous ambulatory peritoneal dialysis (r = 0.77, P< 0.01). Haemodialysis for 4 ;h acutely decreased plasma NO2-/NO3- (92±17 versus 50±8 ;μmol, P< 0.05) and cGMP concentration (16.5±4.3 versus 5.1±1.7 ;pmol/ml, P< 0.01), but did not decrease exhaled nitric oxide output. The increase in exhaled nitric oxide with the simultaneous increase in circulating cytokines suggests that nitric oxide synthase seems to be induced significantly in patients with chronic renal failure. Increased endogenous nitric oxide production may have a pathophysiological role in patients with uraemia.

Serum levels of nitric oxide and endothelin-1 in patients treated with continuous ambulatory peritoneal dialysis

Renal Failure ◽  
2013 ◽  
Vol 36 (3) ◽  
pp. 437-440 ◽  
Author(s):  
Pedja Kovačević ◽  
Saša Dragić ◽  
Zvezdana Rajkovača ◽  
Slavimir Veljković ◽  
Tijana Kovačević
Keyword(s):  
Nitric Oxide ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Serum Levels ◽  
Endothelin 1

EFFECTS OF HAEMODIALYSIS AND CONTINUOUS AMBULATORY PERITONEAL DIALYSIS ON NITRIC OXIDE SERUM CONCENTRATION IN PATIENTS WITH CHRONIC RENAL FAILURE

2008 ◽  
Vol 37 (2) ◽  
pp. 93-98
Author(s):  
Amela Matavulj ◽  
Pedja Kovacevic ◽  
Jasminko Huskic ◽  
Slavimir Veljkovic ◽  
Zvezdana Rajkovaca ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Renal Failure ◽  
Peritoneal Dialysis ◽  
Chronic Renal Failure ◽  
Serum Concentration ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
No Production ◽  
Bleeding Tendency ◽  
Wide Range ◽  
Hemostatic Disorders

Introduction: Nitric oxide (NO) plays an important role in a wide range of physiologic and pathophysiological processes. A major mediator of endothelial function, NO regulates vasodilatory and antithrombotic actions in the vasculature and plays a role in reproductive functions, bronchodilation, bone formation, memory, insulin sensitivity, and gastrointestinal relaxation. Impaired NO bioactivity is strongly associated with endothelial dysfunction. NO, an L-arginine derivative, also exerts a variety of renal and extrarenal physiological and pathophysiological effects. It seems that NO synthetic pathway could have a key role in mediating the complex hemodynamic and hemostatic disorders associated to the progression of renal disease. It remains unclear whether endogenous NO production is increased or decreased in patients with chronic renal failure. The objective of this study was to present the effect of different dialysis treatment on NO serum concentration in patients with chronic renal failure.Patients and Methods: To evaluate endogenous NO production in these patients we studied plasma NO2 and NO3 levels (determined with the Griess method) in patients who underwent regular continuous ambulatory peritoneal dialysis or repeated haemodialysis and in healthy subjects. The study included 51 patients suffering from chronic renal failure and 30 healthy subjects.Results: Our results show that patients with chronic renal failure had a significantly higher NO serum concentration than controls. These values did not differ between patients on haemodialysis and those on continuous ambulatory peritoneal dialysis. NO serum concentration did not differ between female and male independently of the patient’s treatment.Discussion and Conclusion: From obtained results we can concluded that uremia is associated with excessive systemic NO release independently of the patient’s treatment. Alter (increase) NO synthesis may help to explain some pathological changes seen in uraemia such as bleeding tendency, a well-known complication of uremia and hemodialysis hypotension.

Peritoneal nitric oxide is a marker of peritonitis in patients on continuous ambulatory peritoneal dialysis

1996 ◽  
Vol 11 (12) ◽  
pp. 2466-2471 ◽  
Author(s):  
C. W. Yang ◽  
T. L. Hwang ◽  
C. H. Wu ◽  
M. S. Wu ◽  
P. C. Lai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis

Platelets from patients on haemodialysis show impaired responses to nitric oxide

1992 ◽  
Vol 83 (3) ◽  
pp. 313-318 ◽  
Author(s):  
M. P. Gordge ◽  
G. H. Neild
Keyword(s):  
Nitric Oxide ◽  
Peritoneal Dialysis ◽  
Cyclic Amp ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Cyclic Gmp ◽  
Control Subjects ◽  
Healthy Control ◽  
Normal Sensitivity ◽  
Induced Aggregation

1. Platelets from patients on haemodialysis showed a loss of sensitivity to nitric oxide, reflected by a reduction in the ability of nitric oxide both to inhibit thrombin-induced aggregation and to increase intraplatelet cyclic GMP levels. Responses of platelets from patients on continuous ambulatory peritoneal dialysis were slightly, but not significantly, impaired. Platelets from both groups of uraemic patients showed normal sensitivity to the cyclic AMP-dependent inhibitor prostacyclin. 2. Reduced levels of cyclic GMP in response to nitric oxide in platelets from patients on haemodialysis were due to a defect in its generation, rather than to accelerated breakdown. 3. Basal levels of intra-platelet cyclic GMP were significantly increased in both patients on haemodialysis and patients on continuous ambulatory peritoneal dialysis. 4. The activity of nitric oxide was more stable in plasma than in buffer; its survival in plasma from patients on haemodialysis was similar to that in plasma from healthy control subjects.

Scanning Electron Microscopy of Staphylococcus Epidermidis Adherence to Continuous Ambulatory Peritoneal Dialysis Catheters

1985 ◽  
Vol 43 ◽  
pp. 520-521
Author(s):  
William J. Lamoreaux ◽  
David L. Smalley ◽  
Larry M. Baddour ◽  
Alfred P. Kraus
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Staphylococcus Epidermidis ◽  
Sterile Saline ◽  
Intravascular Devices ◽  
Capd Patient ◽  
Scanning Electron

Infections associated with the use of intravascular devices have been documented and have been reported to be related to duration of catheter usage. Recently, Eaton et al. reported that Staphylococcus epidermidis may attach to silastic catheters used in continuous ambulatory peritoneal dialysis (CAPD) treatment. The following study presents findings using scanning electron microscopy (SEM) of S. epidermidis adherence to silastic catheters in an in vitro model. In addition, sections of polyvinyl chloride (PVC) dialysis bags were also evaluated by SEM.The S. epidermidis strain RP62A which had been obtained in a previous outbreak of coagulase-negative staphylococcal sepsis at local hospitals was used in these experiments. The strain produced surface slime on exposure to glucose, whereas a nonadherent variant RP62A-NA, which was also used in these studies, failed to produce slime. Strains were grown overnight on blood agar plates at 37°C, harvested from the surface and resuspended in sterile saline (0.85%), centrifuged (3,000 rpm for 10 minutes) and then washed twice in 0.1 M phosphate-buffered saline at pH 7.0. Organisms were resuspended at a concentration of ca. 106 CFU/ml in: a) sterile unused dianeal at 4.25% dextrose, b) sterile unused dianeal at 1.5% dextrose, c) sterile used dialysate previously containing 4.25% dextrose taken from a CAPD patient, and d) sterile used dialysate previously containing 1.5% dextrose taken from a CAPD patient.

Sign in / Sign up

Export Citation Format

Share Document