Dietary Choline Intake during Pregnancy and PEMT rs7946 Polymorphism on Risk of Preterm Birth: A Case-Control Study

Introduction and Aims: Choline-metabolizing genetic variation may interact with choline intake on fetal programming and pregnancy outcome. This case-control study aims to explore the association of maternal choline consumption and phosphatidylethanolamine N-methyltransferase (PEMT) gene polymorphism rs7946 with preterm birth risk. Methods: 145 Han Chinese women with preterm delivery and 157 Han Chinese women with term delivery were recruited in Shanghai. Dietary choline intake during pregnancy was assessed using a validated food frequency questionnaire. Additionally, DNA samples were genotyped for PEMT rs7946 (G5465A) with plasma homocysteine (Hcy) levels measured. Results: Compared with the lowest quartile of choline intake, women within the highest consumption quartile had adjusted odds ratio (aOR) for preterm birth of 0.48 (95% confidence interval, CI [0.24, 0.95]). There was a significant interaction between maternal choline intake and PEMT rs7946 (p for interaction = 0.04), where the AA genotype carriers who consumed the energy-adjusted choline <255.01 mg/day had aOR for preterm birth of 3.75 (95% CI [1.24, 11.35]), compared to those with GG genotype and choline intake >255.01 mg/day during pregnancy. Additionally, the greatest elevated plasma Hcy was found in the cases with AA genotype and choline consumption <255.01 mg/day (p < 0.001). Conclusion: The AA genotype of PEMT rs7946 may be associated with increased preterm birth in these Han Chinese women with low choline intake during pregnancy.

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Dietary Choline Intake During Pregnancy and PEMT rs7946 Polymorphism on Risk of Preterm Birth: A Case-Control Study

Current Developments in Nutrition ◽

10.1093/cdn/nzaa054_186 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 1114-1114

Author(s):

Jie Zhu ◽

Xiang-Long He ◽

Yu-Hong Liu ◽

Martin Kohlmeier ◽

Bei Wang

Keyword(s):

Preterm Birth ◽

Gene Polymorphism ◽

Case Control Study ◽

Polymerase Chain Reaction Method ◽

Case Control ◽

Chinese Han ◽

Nutrition Research ◽

Choline Intake ◽

Dietary Choline ◽

Control Study

Abstract Objectives Choline-metabolizing genetic variation may interact with choline intake on fetal programming and pregnancy outcome. This case-control study aims to explore the association of maternal choline consumption and phosphatidylethanolamine N-methyltransferase (PEMT) gene polymorphism rs7946 with incident preterm birth. Methods 165 Chinese Han women with preterm delivery and 165 Chinese Han women with term delivery were recruited in Shanghai, China. Dietary choline intake during pregnancy was assessed using a validated food frequency questionnaire. Additionally, DNA samples were genotyped for PEMT rs7946 (G5465A) by a real-time polymerase chain reaction method with plasma homocysteine (Hcy) levels measured by an enzymatic cycling method. Odds ratio (OR) and 95% CI were estimated using unconditional logistic regression with adjustment of the covariates which were selected based on a comparison of social-democratic and clinical characteristics between two groups. Results There was a significant interaction between maternal choline intake and PEMT rs7946 (P for interaction = 0.04), where the AA genotype carriers who consumed the energy-adjusted choline <255.01 mg/d had an adjusted OR for preterm birth of 3.75 (95% CI 1.24–11.35), compared to those with GG genotype and choline intake >255.01 mg/d during pregnancy. Additionally, the highest plasma Hcy was found in the cases with AA genotype and choline consumption <255.01 mg/d (P < 0.001). The AA genotype was also associated with a greater risk of preterm birth among the women when their plasma Hcy is at the highest tertile level (AA vs. GA + GG: adjusted OR = 1.56, 95% CI 1.02–2.88). Conclusions The AA genotype of PEMT rs7946 may be associated with increased preterm birth in these Chinese Han women with low choline intake during pregnancy. The observed association may be partially explained by mechanisms related to the increasing Hcy level induced by PEMT rs7946 gene polymorphism. This study was registered at ClinicalTrials.gov (#NCT02841813). Funding Sources The Danone Nutrition Research and Education Grant (DIC2017–09); Research Project of Shanghai University of Traditional Chinese Medicine (2019LK042).

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A case-control study on risk factors of breast cancer in Han Chinese women

Oncotarget ◽

10.18632/oncotarget.21743 ◽

2017 ◽

Vol 8 (57) ◽

pp. 97217-97230 ◽

Cited By ~ 2

Author(s):

Li-Yuan Liu ◽

Fei Wang ◽

Shu-De Cui ◽

Fu-Guo Tian ◽

Zhi-Min Fan ◽

...

Keyword(s):

Breast Cancer ◽

Risk Factors ◽

Case Control Study ◽

Chinese Women ◽

Case Control ◽

Han Chinese ◽

Control Study

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Pai-1 Gene Variants and COC Use Are Associated with Stroke Risk: A Case-Control Study in the Han Chinese Women

Journal of Molecular Neuroscience ◽

10.1007/s12031-014-0418-0 ◽

2014 ◽

Vol 54 (4) ◽

pp. 803-810 ◽

Cited By ~ 7

Author(s):

Xiaoping Huang ◽

Ying Li ◽

Zhizheng Huang ◽

Chun Wang ◽

Zhenlin Xu

Keyword(s):

Stroke Risk ◽

Case Control Study ◽

Chinese Women ◽

Case Control ◽

Han Chinese ◽

Gene Variants ◽

Pai 1 ◽

Control Study

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Dietary choline and betaine intake, choline-metabolising genetic polymorphisms and breast cancer risk: a case–control study in China

British Journal Of Nutrition ◽

10.1017/s0007114516002956 ◽

2016 ◽

Vol 116 (6) ◽

pp. 961-968 ◽

Cited By ~ 7

Author(s):

Yu-Feng Du ◽

Wei-Ping Luo ◽

Fang-Yu Lin ◽

Zhen-Qiang Lian ◽

Xiong-Fei Mo ◽

...

Keyword(s):

Breast Cancer ◽

Breast Cancer Risk ◽

Cancer Risk ◽

Carbon Metabolism ◽

Case Control Study ◽

Case Control ◽

Choline Intake ◽

One Carbon Metabolism ◽

Dietary Choline ◽

Control Study

AbstractCholine and betaine are essential nutrients involved in one-carbon metabolism and have been hypothesised to affect breast cancer risk. Functional polymorphisms in genes encoding choline-related one-carbon metabolism enzymes, including phosphatidylethanolamine N-methyltransferase (PEMT), choline dehydrogenase (CHDH) and betaine-homocysteine methyltransferase (BHMT), have important roles in choline metabolism and may thus interact with dietary choline and betaine intake to modify breast cancer risk. This study aimed to investigate the interactive effect of polymorphisms in PEMT, BHMT and CHDH genes with choline/betaine intake on breast cancer risk among Chinese women. This hospital-based case–control study consecutively recruited 570 cases with histologically confirmed breast cancer and 576 age-matched (5-year interval) controls. Choline and betaine intakes were assessed by a validated FFQ, and genotyping was conducted for PEMT rs7946, CHDH rs9001 and BHMT rs3733890. OR and 95 % CI were estimated using unconditional logistic regression. Compared with the highest quartile of choline intake, the lowest intake quartile showed a significant increased risk of breast cancer. The SNP PEMT rs7946, CHDH rs9001 and BHMT rs3733890 had no overall association with breast cancer, but a significant risk reduction was observed among postmenopausal women with AA genotype of BHMT rs3733890 (OR 0·49; 95 % CI 0·25, 0·98). Significant interactions were observed between choline intake and SNP PEMT rs7946 (Pinteraction=0·029) and BHMT rs3733890 (Pinteraction=0·006) in relation to breast cancer risk. Our results suggest that SNP PEMT rs7946 and BHMT rs3733890 may interact with choline intake on breast cancer risk.

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