scholarly journals Application of “Tissueoid Cell Culture System” Using a Silicate Fiber Scaffold for Cancer Research

Pathobiology ◽  
2020 ◽  
Vol 87 (5) ◽  
pp. 291-301 ◽  
Author(s):  
Shoko Murakami ◽  
Ken-ichi Mukaisho ◽  
Takuya Iwasa ◽  
Masaaki Kawabe ◽  
Saori Yoshida ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Cancer Cells ◽  
Culture System ◽  
3D Culture ◽  
Collagen Iv ◽  
Cell Culture System ◽  
Adenocarcinoma Cells ◽  
3D Culture System ◽  
Cells Cultured

<b><i>Background:</i></b> We developed a 3-dimensional (3D) culture system using a high-purity silica fiber scaffold of unwoven sheets called Cellbed<sup>TM</sup>. <b><i>Methods:</i></b> We used adherent colon and esophagogastric junction adenocarcinoma cells, tongue squamous cell carcinoma (SqCC) cells, and nonadherent gastric cancer cells. These cells were subjected to staining with various substances and observed by electron microscopy. To evaluate the effects of extracellular matrix in carcinoma tissues, SqCC cells were cultured in Cellbed coated with collagens I, III, and IV. <b><i>Results:</i></b> Especially well-differentiated carcinoma cells cultured in this 3D system showed their own unique characteristics: luminal formation in adenocarcinoma cells and cell stratification and keratinization in SqCC cells. Scanning electron microscopy revealed the proliferation of cancer cells with cytoplasm entwined in Cellbed. Intercellular desmosomes in squamous epithelia were detected by transmission electron microscopy of vertical cross sections. SqCC cells cultured in Cellbed coated with collagen IV showed enhanced invasive and proliferative abilities. <b><i>Conclusion:</i></b> Because the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a “tissueoid cell culture system.” Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment.

scholarly journals Differences in the Central Energy Metabolism of Cancer Cells between Conventional 2D and Novel 3D Culture Systems

2021 ◽  
Vol 22 (4) ◽  
pp. 1805
Author(s):  
Ryo Ikari ◽  
Ken-ichi Mukaisho ◽  
Susumu Kageyama ◽  
Masayuki Nagasawa ◽  
Shigehisa Kubota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Culture System ◽  
3D Culture ◽  
Cell Culture System ◽  
Metabolome Analysis ◽  
Wide Range

The conventional two-dimensional (2D) culture is available as an in vitro experimental model. However, the culture system reportedly does not recapitulate the in vivo cancer microenvironment. We recently developed a tissueoid cell culture system using Cellbed, which resembles the loose connective tissue in living organisms. The present study performed 2D and three-dimensional (3D) culture using prostate and bladder cancer cell lines and a comprehensive metabolome analysis. Compared to 3D, the 2D culture had significantly lower levels of most metabolites. The 3D culture system did not impair mitochondrial function in the cancer cells and produce energy through the mitochondria simultaneously with aerobic glycolysis. Conversely, ATP production, biomass (nucleotides, amino acids, lipids and NADPH) synthesis and redox balance maintenance were conducted in 3D culture. In contrast, in 2D culture, biomass production was delayed due to the suppression of metabolic activity. The 3D metabolome analysis using the tissueoid cell culture system capable of in vivo cancer cell culture yielded results consistent with previously reported cancer metabolism theories. This system is expected to be an essential experimental tool in a wide range of cancer research fields, especially in preclinical stages while transitioning from in vitro to in vivo.

scholarly journals Development of an Innovative 3D Cell Culture System to Study Tumour - Stroma Interactions in Non-Small Cell Lung Cancer Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92511 ◽  
Author(s):  
Arno Amann ◽  
Marit Zwierzina ◽  
Gabriele Gamerith ◽  
Mario Bitsche ◽  
Julia M. Huber ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Culture ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Culture System ◽  
3D Cell Culture ◽  
Small Cell ◽  
Cell Culture System ◽  
Tumour Stroma ◽  
Small Cell Lung

FGF1 and IGF1-conditioned 3D culture system promoted the amplification and cancer stemness of lung cancer cells

Biomaterials ◽  
2017 ◽  
Vol 149 ◽  
pp. 63-76 ◽  
Author(s):  
Pengpeng Liu ◽  
Rui Zhang ◽  
Wenwen Yu ◽  
Yingnan Ye ◽  
Yanan Cheng ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Culture System ◽  
3D Culture ◽  
Lung Cancer Cells ◽  
Cancer Stemness ◽  
3D Culture System

scholarly journals Three-Dimensional Culture System of Cancer Cells Combined with Biomaterials for Drug Screening

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2754 ◽  
Author(s):  
Teruki Nii ◽  
Kimiko Makino ◽  
Yasuhiko Tabata
Keyword(s):  
Cell Culture ◽  
Cancer Cells ◽  
Drug Screening ◽  
Culture System ◽  
Three Dimensional ◽  
3D Culture ◽  
New Drugs ◽  
Biological Research

Anticancer drug screening is one of the most important research and development processes to develop new drugs for cancer treatment. However, there is a problem resulting in gaps between the in vitro drug screening and preclinical or clinical study. This is mainly because the condition of cancer cell culture is quite different from that in vivo. As a trial to mimic the in vivo cancer environment, there has been some research on a three-dimensional (3D) culture system by making use of biomaterials. The 3D culture technologies enable us to give cancer cells an in vitro environment close to the in vivo condition. Cancer cells modified to replicate the in vivo cancer environment will promote the biological research or drug discovery of cancers. This review introduces the in vitro research of 3D cell culture systems with biomaterials in addition to a brief summary of the cancer environment.

Morphology of Various Cancer Cells Using the Silicate Fiber Scaffold Cellbed in a Novel Three-Dimensional Cell Culture System

2017 ◽  
Vol 152 (5) ◽  
pp. S352-S353
Author(s):  
Ken-ichi Mukaisho ◽  
Saori Yoshida ◽  
Masaharu Noi ◽  
Takahisa Nakayama ◽  
Takuya Iwasa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cells ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Dimensional Cell
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