Rapid Cell Transfer by Means of Nylon Mesh to Improve Cellular Diagnosis: The Role of Immunocytochemistry

2021 ◽  
pp. 1-6
Author(s):  
Satoshi Morito ◽  
Takao Nitanda ◽  
Ryuko Tsukamoto ◽  
Shingo Kamoshida ◽  
Hiroshi Yasui ◽  
...  
Keyword(s):  
Needle Aspiration ◽  
Turnaround Time ◽  
Body Cavity ◽  
Cell Transfer ◽  
Special Equipment ◽  
Nylon Mesh ◽  
Cytological Smear ◽  
Liquid Based Cytology ◽  
Transfer Method

Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.

scholarly journals The Role of CD56 in Thyroid Fine Needle Aspiration Cytology: A Pilot Study Performed on Liquid Based Cytology

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132939 ◽  
Author(s):  
Tommaso Bizzarro ◽  
Maurizio Martini ◽  
Carla Marrocco ◽  
Donato D’Amato ◽  
Emanuela Traini ◽  
...  
Keyword(s):  
Pilot Study ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Thyroid Fine Needle Aspiration ◽  
Liquid Based Cytology ◽  
Needle Aspiration Cytology

The role of liquid-based cytology in the investigation of breast lesions using fine-needle aspiration: A cytohistopathological evaluation

2005 ◽  
Vol 89 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Konstantinos Kontzoglou ◽  
Konstantinos G. Moulakakis ◽  
Petros Konofaos ◽  
Mary Kyriazi ◽  
Aspasia Kyroudes ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Breast Lesions ◽  
Fine Needle ◽  
Liquid Based Cytology

scholarly journals To Obtain More With Less: Cytologic Samples With Ancillary Molecular Techniques—The Useful Role of Liquid-Based Cytology

2018 ◽  
Vol 142 (3) ◽  
pp. 299-307 ◽  
Author(s):  
Maurizio Martini ◽  
Sara Capodimonti ◽  
Tonia Cenci ◽  
Mirna Bilotta ◽  
Guido Fadda ◽  
...  
Keyword(s):  
Needle Aspiration ◽  
Molecular Techniques ◽  
Surgical Approaches ◽  
Molecular Testing ◽  
Relevant Role ◽  
Malignant Lesions ◽  
Liquid Based Cytology ◽  
Needle Aspiration Cytology

Context.— Fine-needle aspiration cytology has been increasingly used as the first tool in the evaluation of several diseases. Although cytology has a relevant role in the discrimination between benign and malignant lesions, conventional slides cannot lead to 100% conclusive results. It was hoped that the introduction of liquid-based cytology (LBC) would improve the efficacy of cytology through standardization, quality improvement, and the possibility of carrying out ancillary techniques on the residual stored material. In recent decades, the application of genomic alterations has been studied on cytologic samples with feasible and reliable results. The molecular analysis offers a powerful aid to define the best clinical or surgical approaches and follow-up for patients. In recent years, the application of different ancillary techniques has been carried out on conventional slides even though LBC represents a useful additional and alternative method for molecular testing. Objective.— To demonstrate the relevance of LBC as a valid aid to overcoming the difficulties encountered in the application of ancillary techniques on conventional slides. Data Sources.— We examined and reviewed our experience with the application of ancillary techniques on LBC performed on different body sites. Conclusions.— We emphasize that LBC achieves significant and accurate results. It represents a valid method for cytologic evaluation and it provides highly reproducible and informative molecular yields.

scholarly journals The Utility of Rapid On-Site Evaluation during Bronchoscopic Biopsy: A 2-Year Respiratory Endoscopy Central Experience

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Hansheng Wang ◽  
Na Wei ◽  
Yijun Tang ◽  
Yunyun Wang ◽  
Guoshi Luo ◽  
...  
Keyword(s):  
Lung Cancer ◽  
International Association ◽  
Final Diagnosis ◽  
Needle Aspiration ◽  
European Respiratory Society ◽  
Macroscopic Appearance ◽  
Visible Lesion ◽  
Bronchoscopic Biopsy ◽  
Site Evaluation

Background. Rapid on-site evaluation (ROSE) is commonly used to evaluate the adequacy of biopsy materials in fine-needle aspiration; however, the diagnostic performance of ROSE during fiber optic bronchoscopy (FOB) biopsy under direct vision is rarely reported. Here, we evaluated the role of ROSE during FOB biopsy of visible lesion in trachea or bronchi. Methods. The role of ROSE was prospectively evaluated in consecutive bronchoscopy specimens obtained between January 2016 and January 2018. The agreement and accuracy between ROSE and final histopathological interpretation were assessed. The frequency and possible reasons for discrepancy between ROSE and definitive histopathology results were identified. Histological and cytological classification was performed according to the International Association for the Study of Lung Cancer, the American Thoracic Society, and the European Respiratory Society (IASLC/ATS/ERS) criteria of lung ADCs classification. Results. The study enrolled 651 patients, of which 33 were excluded because of insufficient cells. Final diagnosis of malignancy was achieved in 462 cases (74.8%), whereas 156 cases (25.2%) were nonmalignant. ROSE and pathology were well correlated for the diagnosis of squamous cell carcinoma (SCC) (Kappa = 0.718, p<0.05), adenocarcinoma (AdC) (Kappa = 0.662; p<0.05) and small cell lung cancer (SCLC) (Kappa = 0.955; p<0.05). In 24 cases diagnosed as malignant by ROSE and nonmalignant by pathology, the lesion tissues were surgically excised and re-analyzed, and the 24 cases were finally confirmed as malignant by pathology. Conclusions. ROSE technique allows bronchoscopists to obtain viable and adequate material for the diagnosis of histopathology, and provides them with an onsite preliminary diagnosis especially in cases with inconclusive macroscopic appearance. ROSE and pathology should be used in combination to increase the accuracy of diagnosis.

Turnaround times and modes of reporting critical results in Asian laboratories

2021 ◽  
pp. 000456322198936
Author(s):  
Tony Badrick ◽  
Mohamed Saleem ◽  
Wesley Wong
Keyword(s):  
Quality Improvement ◽  
Performance Index ◽  
Clinical Chemistry ◽  
Reporting Quality ◽  
Turnaround Time ◽  
Timely Manner ◽  
Phone Calls ◽  
Key Performance Index ◽  
Critical Results

Background Reporting critical results in a timely manner is a crucial role of clinical laboratories. Traditionally, these results were reported using the phone or fax system. However, there are now other modes of communication for this reporting. Quality improvement in any organization is driven by detection of errors and benchmarking against peers. In the case of critical result reporting, there are few current widely used Benchmarking schemes. Methods The Roche Clinical Chemistry Benchmarking Survey in 2019 added questions about critical result reporting including the mode of communication and turnaround time key performance index. This survey includes over 1100 laboratories from 20 countries. Results The survey revealed a range of communication strategies with phone calls still the commonest followed by email. The key performance index for most laboratories was less than 10 min. Conclusion Benchmarking can provide key information for quality improvement activities, particularly pre- and postanalytical.

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