Induced Polyspecificity of Human Secretory Immunoglobulin A Antibodies: Is It Possible to Improve Their Ability to Bind Pathogens?

Pharmacology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Ekaterina N. Gorshkova ◽  
Shina Pashova ◽  
Ekaterina A. Vasilenko ◽  
Tatiana S. Tchurina ◽  
Elizaveta A. Razzorenova ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Binding ◽  
Secretory Immunoglobulin A ◽  
Acidic Ph ◽  
Ferrous Ions ◽  
Binding Properties ◽  
Binding Behavior ◽  
Secretory Immunoglobulin

<b><i>Introduction:</i></b> As has been shown previously, various protein-modifying agents can change the antigen-binding properties of immunoglobulins. However, induced polyspecificity of human secretory immunoglobulin A (sIgA) has not been previously characterized in detail. <b><i>Methods:</i></b> In the present study, human secretory immunoglobulin A (IgA) was exposed to buffers with acidic pH, to free heme, or to pro-oxidative ferrous ions, and the antigen-binding behavior of the native and modified IgA to viral and bacterial antigens was compared using Western blotting and enzyme-linked immunosorbent assay. The ability of these agents to modulate the antigen-binding properties of human sIgA toward a wide range of pathogen peptides was investigated using an epitope microarray. <b><i>Results:</i></b> We have shown that acidic pH, heme, and pro-oxidative ferrous ions influenced the binding of secretory IgA in opposite directions (either increasing or decreasing); however, the strongest effect was observed when using buffers with low pH. This fraction had the highest number of affected reactivities; most of them were increased and most of the new ones were toward common pathogens. <b><i>Conclusions:</i></b> Thus, it was shown that all investigated treatments can alter to some degree the antigen-binding of secretory IgA, but acidic pH has the most potentially beneficial effect by increasing binding to a largest number of common pathogens’ antigens.

scholarly journals Antigen Binding Properties of Purified Immunoglobulin A and Reconstituted Secretory Immunoglobulin A Antibodies

1996 ◽  
Vol 271 (27) ◽  
pp. 16300-16309 ◽  
Author(s):  
Elke Lüllau ◽  
Stephan Heyse ◽  
Horst Vogel ◽  
Ian Marison ◽  
Urs von Stockar ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Antigen Binding ◽  
Secretory Immunoglobulin A ◽  
Binding Properties ◽  
Secretory Immunoglobulin

scholarly journals Correlations between Antibody Immune Responses at Different Mucosal Effector Sites Are Controlled by Antigen Type and Dosage

2000 ◽  
Vol 68 (7) ◽  
pp. 3830-3839 ◽  
Author(s):  
Dörthe Externest ◽  
Barbara Meckelein ◽  
M. Alexander Schmidt ◽  
Andreas Frey
Keyword(s):  
Cholera Toxin ◽  
Plasma Cells ◽  
Immunoglobulin A ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Routine Diagnostics ◽  
Significant Relationships ◽  
Serum Igg ◽  
Mucosal Surfaces ◽  
Secretory Immunoglobulin

ABSTRACT Monitoring specific secretory immunoglobulin A (IgA) responses in the intestines after mucosal immunization or infection is impeded by the fact that sampling of small intestinal secretions requires invasive methods not feasible for routine diagnostics. Since IgA plasma cells generated after intragastric immunization are known to populate remote mucosal sites as well, secretory IgA responses at other mucosal surfaces may correlate to those in the intestines and could serve as proxy measures for IgA secretion in the gut. To evaluate the practicability of this approach, mice were immunized intragastrically with 0.2, 2, and 20 mg of ovalbumin plus 10 μg of cholera toxin, and the antigen-specific local secretory IgA responses in duodenal, ileal, jejunal, rectal, and vaginal secretions, saliva, urine, and feces, as well as serum IgG and IgA responses were analyzed by enzyme-linked immunosorbent assay. Correlation analysis revealed significant relationships between serum IgG and IgA, urinary IgA, salivary IgA, and secretory IgA in duodenal, jejunal, ileal, and rectal secretions for the 0.2-mg but not for the 20-mg ovalbumin dose. Fecal samples were poor predictors for intestinal antiovalbumin IgA responses, and no correlations could be established for cholera toxin, neither between local anti-cholera toxin levels nor to the antiovalbumin responses. Thus, specific IgA in serum, saliva, or urine can serve as a predictor of the release of specific IgA at intestinal surfaces after intragastric immunization, but the lack of correlations for high ovalbumin doses and for cholera toxin indicates a strong dependency on antigen type and dosage for these relationships.

scholarly journals Fecal Immunoglobulin A Antibodies in Dogs Infected or Vaccinated with Canine Coronavirus

2004 ◽  
Vol 11 (1) ◽  
pp. 102-105 ◽  
Author(s):  
Nicola Decaro ◽  
Annamaria Pratelli ◽  
Antonella Tinelli ◽  
Vito Martella ◽  
Michele Camero ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inactivated Vaccine ◽  
Secretory Immunoglobulin A ◽  
Intramuscular Route ◽  
Degree Of Protection ◽  
Canine Coronavirus ◽  
Iga Antibodies ◽  
Secretory Immunoglobulin

ABSTRACT Fecal secretory immunoglobulin A (IgA) antibodies in dogs infected or vaccinated with canine coronavirus (CCV) were evaluated by an enzyme-linked immunosorbent assay. The study was carried out with 32 fecal samples collected just before inoculation and at 28 days postinoculation. Five groups were studied: naturally infected dogs, experimentally infected dogs, dogs inoculated with a modified live (ML) CCV vaccine by the intramuscular route, dogs inoculated with an ML CCV vaccine by the oronasal route, and dogs given an inactivated CCV vaccine. Both the naturally and the experimentally infected dogs developed high levels of fecal IgAs. Interestingly, dogs inoculated with the ML CCV vaccine by the oronasal route developed levels of fecal IgA that were higher than those observed in the dogs inoculated with the same CCV vaccine by the intramuscular route or those observed in dogs inoculated with the inactivated vaccine. A relationship between the level of fecal IgAs to CCV and the degree of protection against CCV infection was observed.

scholarly journals Analysis of Occupational Stress and Its Relationship with Secretory Immunoglobulin A in the Xinjiang Plateau Young Military Recruits

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ning Tao ◽  
Hengqing An ◽  
Jianjiang Zhang ◽  
Yuanyue Zhang ◽  
Lu Jin ◽  
...  
Keyword(s):  
Occupational Stress ◽  
Military Training ◽  
Immunoglobulin A ◽  
Demographic Characteristics ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Stress Group ◽  
Coping Ability ◽  
Military Recruits ◽  
Secretory Immunoglobulin

Background. With the continuous improvement of the modernization of the Chinese military and the major adjustments made by the state to the recruitment policy, the newly recruited military undergone multiple pressures such as targeted high-intensity military training and environmental changes. The mental health of military has become a crucial factor of improving the fighting capacity effectiveness of the troops. Objectives. To explore occupational stress of young recruits in the Xinjiang plateau environment during their basic military training period and analyze the relationship between occupational stress and secretory immunoglobulin A (sIgA) levels. Methods. Using multistage stratified cluster random sampling, 625 recruits stationed at Xinjiang plateau command in 2014 were enrolled as subjects. Occupational stress was assessed by the Occupational Stress Inventory Revised Edition (OSI-R). sIgA in saliva was quantified by enzyme-linked immunosorbent assay. The resulting data were analyzed using descriptive statistics, nonparametric tests, and correlation analysis. Results. Based on demographic characteristics, occupational stress was higher in the urban group than the rural group, coping ability for stress was greater in individuals who were students before joining the army than nonstudents, occupational stress was higher in smokers than nonsmokers, and coping ability for stress was higher in nonsmokers than in smokers (all P<0.05). Being an only child, educational level and age were not significantly related to occupational stress scores (P>0.05). Salivary sIgA level was higher in the high occupational stress group than in the low stress group (P<0.01). Salivary sIgA was positively correlated with scores on the occupational role and personal strain questionnaires (rs=0.229, rs=0.268, P<0.01). Conclusion. Demographic characteristics influenced occupational stress among young recruits in cold and high-altitude area. Further, there were some relationships between occupational stress and salivary sIgA in young military recruits.

Interaction of secretory immunoglobulin A antibodies withNaegleria fowleritrophozoites and collagen type I

2003 ◽  
Vol 49 (3) ◽  
pp. 164-170 ◽  
Author(s):  
Mineko Shibayama ◽  
José de Jesús Serrano-Luna ◽  
Saúl Rojas-Hernández ◽  
Rafael Campos-Rodríguez ◽  
Víctor Tsutsumi
Keyword(s):  
Collagen Type ◽  
Immunoglobulin A ◽  
Collagen Type I ◽  
Secretory Iga ◽  
Type I ◽  
Secretory Immunoglobulin A ◽  
Naegleria Fowleri ◽  
Nægleria Fowleri ◽  
Secretory Immunoglobulin

In this work, we analyzed the in vitro interaction of human secretory immunoglobulin A (sIgA) antibodies with Naegleria fowleri trophozoites and the capacity of these antibodies to inhibit amoeba adherence to collagen type I. We also studied N. fowleri antigens that are recognized by sIgA, using immunoblot assays. Immunocytochemical analysis of the interaction showed a redistribution of antigens on the surface of trophozoites by sIgA antibodies. Ultrastructural analysis of antibody–amoeba interaction showed that besides the patching and cap formation, parasites were capable of eliminating the antigen–antibody complex produced on the surface. sIgA antibodies were capable of inhibiting the in vitro adhesion of trophozoites to collagen type I. We suggest that nonsymptomatic infections by N. fowleri may stimulate a local specific immunity that prevents trophozoite adhesion and invasion of nasal mucosa.Key words: Naegleria fowleri, free-living amoebae, human antibodies, secretory IgA, mucosal immunity.

scholarly journals The sIgA and histatin-5 expressions in saliva of early childhood caries and caries-free children based on period times

2021 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Dharli Syafriza ◽  
Heriandi Sutadi ◽  
Ameta Primasari ◽  
Yahwardiyah Siregar
Keyword(s):  
Early Childhood ◽  
Early Childhood Caries ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Weak Correlation ◽  
Histatin 5 ◽  
Significant Difference ◽  
Secretory Immunoglobulin ◽  
Childhood Caries

To analyze the level of sIgA and Histatin-5 expression in Early Childhood Caries (ECC) and caries-free children. Saliva was collected from 31 subjects of ECC and 29 caries-free (ages 9-32 months). The level expression of sIgA and Histatin 5 was analyzed by enzyme-linked immunosorbent assay. The distribution and frequency of subjects consist of boys 47% and girls 53% with the average of ages 1-12 month 21% and 13-24 month 79%.sIgA and Histatin 5 expressions differ from the month periods tested (1, 3, and 9 months) but do not show any significant difference from each examination period (1 with three months; p0.05: 0.116), (1 with nine months; p 0.05: 0.766), and (3 to 9 months; p0.05: 0.398). The expression of sIgA and Histatin 5 of boys and girls were not significant (p0.05:0.550) and weak correlation (r = 0.079).The level expression of sIgA in the Caries-free higher than ECC (p0.05). Whereas the expression of Histatin 5 in ECC higher than Caries-free (p0.05). The sIgA correlating in ECC and caries-free is significant (p0.05) and no correlation (r =- 0,29). Whereas Histatin 5 in the ECC and caries-free were strong correlation = 0,84. Both the result of ECC and caries-free suggested that the antibodies have been involved in the caries pathogenesis. In the ECC cases that occurred to decrease sIgA levels and increased of Histatin 5 levels, both antibodies have expressed in Caries-free Children. Both sIgA and Histatin 5 involved in the pathogenesis caries infection in children.KEYWORDS: Antibodies; Early Childhood Caries; saliva; secretory immunoglobulin A; Histatin-5

scholarly journals Study of Humoral Immunity to Commensal Oral Bacteria in Human Infants Demonstrates the Presence of Secretory Immunoglobulin A Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 Ribotypes

2004 ◽  
Vol 11 (3) ◽  
pp. 473-482 ◽  
Author(s):  
Michael F. Cole ◽  
Mishell K. Evans ◽  
Jennifer L. Kirchherr ◽  
Michael J. Sheridan ◽  
G. H. W. Bowden
Keyword(s):  
Cell Wall ◽  
Immunoglobulin A ◽  
Oral Bacteria ◽  
Enzyme Linked Immunosorbent Assay ◽  
Secretory Immunoglobulin A ◽  
Cell Wall Proteins ◽  
Human Infants ◽  
Specific Antibodies ◽  
Actinomyces Naeslundii ◽  
Secretory Immunoglobulin

ABSTRACT The mouths of three human infants were examined from birth to age 2 years to detect colonization of Actinomyces naeslundii genospecies 1 and 2. These bacteria did not colonize until after tooth eruption. The diversity of posteruption isolates was determined by ribotyping. Using immunoblotting and enzyme-linked immunosorbent assay, we determined the reactivity of secretory immunoglobulin A (SIgA) antibodies in saliva samples collected from each infant before and after colonization against cell wall proteins from their own A. naeslundii strains and carbohydrates from standard A. naeslundii genospecies 1 and 2 strains. A. naeslundii genospecies 1 and 2 carbohydrate-reactive SIgA antibodies were not detected in any saliva sample. However, SIgA antibodies reactive with cell wall proteins were present in saliva before these bacteria colonized the mouth. These antibodies could be almost completely removed by absorption with A. odontolyticus, a species known to colonize the human mouth shortly after birth. However, after colonization by A. naeslundii genospecies 1 and 2, specific antibodies were induced that could not be removed by absorption with A. odontolyticus. Cluster analysis of the patterns of reactivity of postcolonization salivary antibodies from each infant with antigens from their own strains showed that not only could these antibodies discriminate among strains but antibodies in saliva samples collected at different times showed different reactivity patterns. Overall, these data suggest that, although much of the salivary SIgA antibodies reactive with A. naeslundii genospecies 1 and 2 are directed against genus-specific or more broadly cross-reactive antigens, species, genospecies, and possibly strain-specific antibodies are induced in response to colonization.

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