scholarly journals The gilled mushroom Amanita spissacea (Amanitaceae): a new report for India

2018 ◽  
Vol 10 (10) ◽  
pp. 12413-12417
Author(s):  
Hmar Lalrinawmi ◽  
John Zothanzama ◽  
Benjamin W. Held ◽  
Josiah M.C. Vabeikhokhei ◽  
Zohmangaiha . ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Systematic Study ◽  
Field Survey ◽  
Its Region ◽  
Distinct Species ◽  
Biodiversity Hotspots ◽  
Diverse Group ◽  
Closely Related Species ◽  
The World

Mizoram is regarded as one of the biodiversity hotspots of the World owing to the diverse group of flora and fauna documented here.  Information regarding the macrofungi, however, is very limited.  For this reason, a systematic study of mushrooms from Mizoram was undertaken and during the field survey, Amanita spissacea was collected and identified.  This is the first report of this mushroom from India.  This species was identified on the basis of its morphological and microscopic characteristics as well as molecular characterization of the ITS region of rDNA.  Phylogenetic analysis also confirmed that A. spissacea was a distinct species from A. fritillaria, A. sepiacea, A. citrina and other closely related species Amanita section Valideae.

Two new rock-inhabiting species of Cyphellophora from karst habitats in China

Phytotaxa ◽  
2019 ◽  
Vol 397 (1) ◽  
pp. 23
Author(s):  
WEI SUN ◽  
BINGJIE LIU ◽  
RONG FU ◽  
XINGZHONG LIU ◽  
MEICHUN XIANG
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Its Region ◽  
Closely Related Species ◽  
Two New Species ◽  
Rock Inhabiting Fungi ◽  
Karst Habitats

During survey on rock-inhabiting fungi from karst habitats in Guizhou, China, two new species in Cyphellophora were discovered and identified. Phylogenetic analysis based on combined sequences of the nuclear large subunit (nucLSU) and internal transcribed spacer (ITS) region of ribosomal DNA revealed that the tested isolates, clustered into two clades that well affiliated in the genus of Cyphellophora. Morphological characteristics were also supported the estabolishment of the new species. Herewith Cyphellophora botryose sp. nov. and Cyphellophora guizhouensis sp. nov. were described and their differences from closely related species were discussed.

scholarly journals Characterization of antigenically and genetically similar influenza C viruses isolated in Japan during the 1999–2000 season

2004 ◽  
Vol 132 (4) ◽  
pp. 709-720 ◽  
Author(s):  
Y. MATSUZAKI ◽  
S. TAKAO ◽  
S. SHIMADA ◽  
K. MIZUTA ◽  
K. SUGAWARA ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Composition ◽  
Antigenic Analysis ◽  
The World ◽  
Influenza C Virus

Between October 1999 and May 2000, a total of 28 strains of influenza C virus were isolated in four Japanese prefectures: Yamagata, Miyagi, Saitama and Hiroshima. Antigenic analysis showed that the 28 isolates were divided into three distinct antigenic groups, and viruses belonging to different antigenic groups were co-circulating in each of the four prefectures. Phylogenetic analysis of the seven protein genes demonstrated that the viruses having a similar genome composition spread in various areas of Japan during the same period. Furthermore, phylogenetic analysis showed that most of the influenza C viruses isolated in various areas of the world between the 1970s and 1980s were closely related to the contemporary Japanese viruses in all gene segments. These observations suggest that the influenza C viruses cause epidemics in some communities during the same season and that antigenically and genetically similar influenza C viruses spread throughout Japan and may be circulating worldwide.

scholarly journals Occurrence and Characterization of a Phytophthora sp. Pathogenic to Asparagus (Asparagus officinalis) in Michigan

2008 ◽  
Vol 98 (10) ◽  
pp. 1075-1083 ◽  
Author(s):  
C. Saude ◽  
O. P. Hurtado-Gonzales ◽  
K. H. Lamour ◽  
M. K. Hausbeck
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Mycelial Growth ◽  
Fragment Length Polymorphism ◽  
Distinct Species ◽  
Asparagus Officinalis ◽  
Polymorphism Analysis ◽  
Clonal Lineage ◽  
Storage Roots

A homothallic Phytophthora sp. was recovered from asparagus (Asparagus officinalis) spears, storage roots, crowns, and stems in northwest and central Michigan in 2004 and 2005. Isolates (n = 131) produced ovoid, nonpapillate, noncaducous sporangia 45 μm long × 26 μm wide and amphigynous oospores of 25 to 30 μm diameter. Mycelial growth was optimum at 25°C with no growth at 5 and 30°C. All isolates were sensitive to 100 ppm mefenoxam. Pathogenicity studies confirmed the ability of the isolates to infect asparagus as well as cucurbits. Amplified fragment length polymorphism analysis of 99 isolates revealed identical fingerprints, with 12 clearly resolved fragments present and no clearly resolved polymorphic fragments, suggesting a single clonal lineage. The internal transcribed spacer regions of representative isolates were homologous with a Phytophthora sp. isolated from diseased asparagus in France and a Phytophthora sp. from agave in Australia. Phylogenetic analysis supports the conclusion that the Phytophthora sp. isolated from asparagus in Michigan is a distinct species, and has been named Phytophthora asparagi.

scholarly journals Molecular characterization of goose parvovirus in geese of Turkey

2021 ◽  
Vol 41 ◽  
Author(s):  
Oya Bulut ◽  
Irmak Dik ◽  
Hatice P. Aslim ◽  
Cagri Avci ◽  
Hasan S. Palanci ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
High Morbidity ◽  
Viral Pathogen ◽  
Virulent Strains ◽  
The World ◽  
Goose Parvovirus ◽  
Pcr Method ◽  
Definition Of

ABSTRACT: Goose parvovirus (GPV), also called Derzsy’s disease, is a viral pathogen that causes high morbidity and mortality in goslings and ducklings. In this study, we perform the molecular characterization of the GPV in Turkey. The definition of similarity to the world of GPV isolates in Turkey and construction of a phylogenetic tree was aimed. For this purpose, the presence of GPV in the liver, spleen, and intestine tissues of nine goslings with symptoms such as dysphagia, bilateral ocular swelling, eye discharge, diarrhea, and fatigue were investigated by real-time PCR method and all samples were detected as positive. According to the data obtained by molecular characterization, phylogenetic analysis of GPV has been presented in Turkey. As a result of this study, it was determined that the GPVs available in Turkey are virulent strains.

scholarly journals Identification and Characterization of Rhizoctonia Species Associated with Soybean Seedling Disease

Plant Disease ◽  
2017 ◽  
Vol 101 (4) ◽  
pp. 520-533 ◽  
Author(s):  
Olutoyosi O. Ajayi-Oyetunde ◽  
Carl A. Bradley
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Relatedness ◽  
Sweet Corn ◽  
Management Practice ◽  
Its Region ◽  
Anastomosis Group ◽  
Seedling Disease ◽  
Binucleate Rhizoctonia ◽  
Soybean Seedlings

In an effort to identify the Rhizoctonia spp. associated with seedling diseases of soybean, Rhizoctonia isolates were recovered from soybean seedlings with damping off and root and hypocotyl rot symptoms from Arkansas, Illinois, Kansas, Michigan, Minnesota, and the Canadian province of Ontario between 2012 and 2014. Based on cultural morphology, polymerase chain reaction restriction fragment length polymorphism, and phylogenetic analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA genes, 80 isolates were confirmed to be Rhizoctonia solani, 24 were binucleate Rhizoctonia spp., and 10 were R. zeae. Of the 80 R. solani isolates, one belonged to anastomosis group (AG) 2-1, 52 belonged to AG-2-2IIIB, five belonged to AG-3 PT, three belonged to AG-4 HGI, two belonged to AG-4 HGIII, nine belonged to AG-7, and eight belonged to AG-11. Bayesian inference of phylogeny using the ITS region revealed two clades of R. solani AG-7 that possibly correspond to different AG-7 subgroups. Phylogenetic analysis also provided evidence for genetic relatedness between certain binucleate Rhizoctonia and some R. solani isolates. On ‘Williams 82’ soybean, isolates of AG-2-2IIIB were the most aggressive, followed by isolates of AG-7, AG-4, and AG-11. On ‘Jubilee’, a sweet corn cultivar, AG-2-2IIIB and AG-4 isolates caused significant stunting and root damage, whereas the damage caused by the AG-11 isolates was mostly restricted to the mesocotyl. Isolates of R. zeae and the binucleate Rhizoctonia spp. were not pathogenic on soybean or corn. Our results indicate that soybean and corn are hosts to the predominant and aggressive AG of R. solani, implying that rotation between these two crops may not be an effective management practice.

Rickenella danxiashanensis, a new bryophilous agaric from China

Phytotaxa ◽  
2018 ◽  
Vol 350 (3) ◽  
pp. 283 ◽  
Author(s):  
MING ZHANG ◽  
TAI-HUI LI ◽  
FANG CHEN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Distinct Species ◽  
Fungal Species ◽  
Taxonomic Position

A new fungal species growing on moss bed, Rickenella danxiashanensis, is described, illustrated and compared with phenotypically similar and phylogenetically related species. Morphologically, the new species is characterized by its omphalinoid basidiomata, yellow to deep orange pileus, decurrent lamellae, abundant lageniform to obclavate cystidia on cap and stipe surface, and small basidiospores (3.5–)4–6 × 3–4 μm. Phylogenetic analysis based on the internal transcribed spacer (ITS) region recognized it as a distinct species, which contribute to settle its taxonomic position in genus Rickenella.

scholarly journals Philogenetic Analysis of S. Scabiei Var. Hominis, Var. Cuniculi, and Var. Marmoota

2021 ◽  
Vol 5 (3) ◽  
pp. 838-842
Author(s):  
Gita Dwi Prasasty ◽  
Miftahurrizqiyah ◽  
Dalilah ◽  
Dwi Handayani ◽  
Tia Sabrina ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Phylogenetic Analysis ◽  
Genetic Variation ◽  
Mortality Rate ◽  
Genetic Information ◽  
Distinct Species ◽  
Global Burden ◽  
Its Sequencing ◽  
The World ◽  
Very High

Introduction: Based on data from the 2017 Global Burden of Disease, it is known that Indonesia is the country with the highest number of scabies incidences out of 195 countries in the world. In addition to humans, scabies is also an infectious disease in mammals with a prevalence of 300 million infestations every year, even in animals the mortality rate from scabies is very high. Until now, it is still unclear whether there is a cross-infestation between human and animal scabies. Morphologically, S. scabiei between variants cannot be distinguished, while research on genetic variation is still not much done. Therefore, it is necessary to conduct studies to enrich genetic information and phylogenetic analysis. Methods: The samples tested were positive PCR results for ITS 2 and CO 1 genes, each on five amplicons, namely three S. scabiei var. hominis, one var. cuniculi, and one var. marmoota. Phylogenetics were arranged based on the closeness of the base arrangement in variants and geographically available in NCBI. Results: Of the five sample isolates studied, the ITS 2 gene amplicon is located in the 417bp band and the phylogenetic analysis of its sequencing has two branches that are unable to distinguish var. hominis and var. animalia. The CO1 gene has electrophoretic results at 317 bp as well as phylogenetic analysis results that specifically divide branches between var. hominis and var. animalia. Conclusion: S. scabiei var. hominis and var. marmoota, and var. cuniculi are distinct species and are host monospecific.

scholarly journals Diversity and Molecular Characterization of Endophytic fungi associated with Leaves of Acacianilotica

2021 ◽  
Vol 18 (2) ◽  
pp. 263-269
Author(s):  
Jawed Shaikh ◽  
Ashfaque Mehboob Khan ◽  
Mirza Mushtaq Vaseem Baig
Keyword(s):  
Molecular Characterization ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Sequence Similarity ◽  
Its Region ◽  
Bioactive Metabolites ◽  
Diverse Group ◽  
Colonization Frequency ◽  
Biodiversity Analysis

Fungal endophytesare colonized in different part of the plants and play important role in survival of plants in stressful habitat. In search of potential endophytic fungito produce bioactive metabolites inthis study we investigate thediversity of endophytic fungi associated with leaves of the Acacia nilotica plant. Twenty-six endophytic fungi were subjected to morphologicaland molecular identification with internal transcribes spacer (ITS) region sequenced. All 26 endophytic fungi were divided into nine genera Chaetomium, Amesia, Ovatospora, Penicillium, Phialemonium, Colletotrichum, Crinipellis, Acrophialophora, Cribbea. Most of them belonged to the phylum Ascomycota only one belonging to the phylum Basidiomycota. This study shows that Acacia leaves inhabitant by diverse group of endophytic fungi. The biodiversity analysis showed Chaetomium sp. Being dominant with the highest colonization frequency (26.9%). One of the Chaetomium sp. showed sequence similarity of93% with the species reported earlier, Further investigationsarein needed to harness the bioactive compounds.

scholarly journals Philogenetic Analysis of S. Scabiei Var. Hominis, Var. Cuniculi, and Var. Marmoota

2021 ◽  
Vol 5 (10) ◽  
pp. 911-915
Author(s):  
Gita Dwi Prasasty ◽  
Miftahurrizqiyah ◽  
Dalilah ◽  
Dwi Handayani ◽  
Tia Sabrina ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Phylogenetic Analysis ◽  
Genetic Variation ◽  
Mortality Rate ◽  
Genetic Information ◽  
Distinct Species ◽  
Global Burden ◽  
Its Sequencing ◽  
The World ◽  
Very High

Introduction: Based on data from the 2017 Global Burden of Disease, it is known that Indonesia is the country with the highest number of scabies incidences out of 195 countries in the world. In addition to humans, scabies is also an infectious disease in mammals with a prevalence of 300 million infestations every year, even in animals the mortality rate from scabies is very high. Until now, it is still unclear whether there is a cross-infestation between human and animal scabies. Morphologically, S. scabiei between variants cannot be distinguished, while research on genetic variation is still not much done. Therefore, it is necessary to conduct studies to enrich genetic information and phylogenetic analysis. Methods: The samples tested were positive PCR results for ITS 2 and CO 1 genes, each on five amplicons, namely three S. scabiei var. hominis, one var. cuniculi, and one var. marmoota. Phylogenetics were arranged based on the closeness of the base arrangement in variants and geographically available in NCBI. Results: Of the five sample isolates studied, the ITS 2 gene amplicon is located in the 417bp band and the phylogenetic analysis of its sequencing has two branches that are unable to distinguish var. hominis and var. animalia. The CO1 gene has electrophoretic results at 317 bp as well as phylogenetic analysis results that specifically divide branches between var. hominis and var. animalia. Conclusion: S. scabiei var. hominis and var. marmoota, and var. cuniculi are distinct species and are host monospecific.

scholarly journals Molecular Characterization of Cryptosporidium molnari Reveals a Distinct Piscine Clade

2010 ◽  
Vol 76 (22) ◽  
pp. 7646-7649 ◽  
Author(s):  
Oswaldo Palenzuela ◽  
Pilar Alvarez-Pellitero ◽  
Ariadna Sitjà-Bobadilla
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Distinct Species ◽  
Small Subunit ◽  
Ssu Rrna

ABSTRACT Multilocus phylogenetic analysis of small-subunit (SSU) rRNA and actin from Cryptosporidium molnari clustered this species with the C. molnari-like genotype of an isolate from the guppy, although the two fish isolates seem to be distinct species. The analysis of available piscine genotypes provides some support for cladistic congruence of the genus Piscicryptosporidium, but additional piscine genotypes are needed.

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