scholarly journals Evidence for Intracellular Endothelin-Converting Enzyme-2 Expression in Cultured Human Vascular Endothelial Cells

1999 ◽  
Vol 84 (8) ◽  
pp. 891-896 ◽  
Author(s):  
Fraser D. Russell ◽  
Anthony P. Davenport
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Converting Enzyme ◽  
Endothelin Converting Enzyme

scholarly journals Localization of rat endothelin-converting enzyme to vascular endothelial cells and some secretory cells

1995 ◽  
Vol 311 (2) ◽  
pp. 657-665 ◽  
Author(s):  
M Takahashi ◽  
K Fukuda ◽  
K Shimada ◽  
K Barnes ◽  
A J Turner ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Vascular Endothelial Cells ◽  
Simian Virus 40 ◽  
Secretory Cells ◽  
Rat Tissues ◽  
Vascular Endothelial ◽  
Converting Enzyme ◽  
Reducing Conditions ◽  
Endothelin Converting Enzyme

Endothelin is a potent vasoconstrictive peptide that is produced by vascular endothelial cells; it is formed from its precursor, big endothelin, by endothelin-converting enzyme (ECE). In this work, ECE was studied using specific monoclonal antibodies. In immunoblotting, ECE was estimated to be a 300 kDa protein on SDS/PAGE under non-reducing conditions, and 130 kDa under reducing conditions. Cross-linking experiments revealed that ECE is composed of two disulphide-linked subunits. Localization of ECE was studied at the cellular and subcellular levels in various rat tissues and cells. High-level expression of ECE was observed in membrane fractions of simian virus 40-transformed rat endothelial cells by immunoblotting, but the immunoreactive band was absent form aortic smooth muscle cells and cytosolic fractions of endothelial cells. In immunohistochemical analysis, ECE was found to be localized in the endothelial cells of the aorta, lung, kidney, liver and heart. Confocal immunofluorescent microscopy showed that most of the ECE in endothelial cells and cells transfected with ECE cDNA was clustered along the plasma membrane. Intact COS or CHO cells transfected with ECE cDNA rapidly and efficiently cleaved big endothelin-1 added to the culture medium. Thus endothelial cells express ECE on the plasma membrane and the active site of the enzyme faces outside the cells, i.e. it is an ectoenzyme. Other than endothelial cells, ECE was also present in some secretory cells. The enzyme was abundant in the adrenal gland, and localized in chromaffin cells. ECE was also highly condensed in pancreatic islet beta cells. It is concluded that ECE and endothelin may be involved in the regulated secretion of hormones.

scholarly journals Anticoagulant Therapy for Patients with Coronavirus Disease 2019: Urgent Need for Enhanced Awareness

2020 ◽  
Vol 15 ◽  
Author(s):  
Maki Komiyama ◽  
Koji Hasegawa
Keyword(s):  
Endothelial Cells ◽  
Anticoagulant Therapy ◽  
Retrospective Studies ◽  
Vascular Endothelial Cells ◽  
Therapeutic Option ◽  
Cardiovascular Complications ◽  
Vascular Endothelial ◽  
Converting Enzyme ◽  
Angiotensin Converting Enzyme 2 ◽  
Global Pandemic

Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic. SARS-CoV-2 binds to the angiotensin-converting enzyme 2 receptor, which is abundantly expressed in vascular endothelial cells and damages these cells. Besides pneumonia-induced respiratory failure, thrombotic cardiovascular complications are increasingly emerging as a major COVID-19 symptom. Multiple retrospective studies have strongly suggested that anticoagulant therapy improves the prognosis of people with COVID-19. However, validation of the safety and effectiveness of anticoagulant therapy for COVID-19 and greater awareness of this clinical therapeutic option are urgently needed.

scholarly journals Prostacyclin generation by cultured human vascular endothelial cells with reference to angiotensin I-converting enzyme.

1986 ◽  
Vol 50 (3) ◽  
pp. 242-247 ◽  
Author(s):  
SHOHEI SAWADA ◽  
TAKEO TOYODA ◽  
HAJIME TAKAMATSU ◽  
ISAMU NIWA ◽  
NORIHIKO MAEBO ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Angiotensin I Converting Enzyme
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