Abstract 078: Differential Phosphorylation of Inositol 1,4,5-triphophate Receptor by CaMKIId During Cardiac Remodeling
Natesan Sankar 1 , Sukriti Dewan 1 , Joshua Maxwell 1 , Donald Bers 2 , Joan Heller Brown 3 , Jeffery Molkentin 4 , Pieter deTombe 1 , Gregory Mignery 1 . 1 Department of Cell and Molecular Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL. 2 Department of Physiology, Department of Pharmacology University of California at Davis, Davis, CA. 3 Department of Pharmacology, University of California, San Diego, La Jolla, CA. 4 Howard Hughes Medical Institute, Molecular Cardiovascular Biology, Cincinnati Children’s Hospital, Cincinnati, OH. In cardiac myocytes the type-2 isoform of the inositol 1,4,5-triphosphate receptor (InsP 3 R2) Ca 2+ release channel is expressed predominantly in the nuclear envelope. InsP 3 R2 releases intracellular Ca 2+ bidirectionally towards the cytoplasm and nucleoplasm in response to an array of pro-hypertrophic signaling. Thus, InsP 3 R2 mediated Ca 2+ release may contribute to both Excitation-Contraction Coupling (ECC) and Excitation-Transcription Coupling (ETC) during normal and pathophysiologic conditions such as cardiac remodeling. However, the regulation of InsP 3 R2 mediated Ca 2+ release and its role in ECC and ETC during cardiac remodeling is not fully understood. We have shown that CaMKIIδ and InsP 3 R2 forms a signaling complex in the heart and CaMKII mediated phosphorylation of InsP 3 R2 at S150 modulates its intrinsic Ca 2+ channel activity. Here we show that InsP 3 R2 is differentially phosphorylated by CaMKIIδ B and CaMKIIδ C , the predominant nucleoplasmic and cytoplasmic isoforms respectively, in cardiac myocytes. Using adult rat cardiac myocytes we show that the differential phosphorylation by CaMKII of InsP 3 R2 at S150 leads to elevated nuclear Ca 2+ signaling and diminished release towards the cytoplasm. Additionally we show that the InsP 3 R2 was phosphorylated in the hearts of Angiotensin II infused and pressure overload induced cardiac remodeling animal models. Finally, we show that there was an increase in InsP 3 R2 phosphorylation in human heart-failure samples compared to non-failing hearts. Collectively our studies demonstrate that, CaMKIIδ mediated regulation of InsP 3 R2 Ca 2+ channel activity contributes to ECC and ETC during all the phases of cardiac remodeling processes.