Abstract 351: Biophysical Cues From Nano Size Pillar Affect Character Of Human Endothelial Colony Forming Cells

Background: Recently, biophysical cues from nano patterned surface received extra attention. Because, numerous cells in the human body is surrounded by the nano-microenvironment. Especially for the live cells biophysical cues from nanotopography is an important factor for cell motility and pathophysiology. Human Endothelial Colony Forming Cells (hECFCs) is human peripheral blood mononuclear cells (PBMNCs) derived endothelial cell like cells which related with various disease occurrence. Methods: To investigate the effect of biophysical cues from nano size pillar surface, we use the novel nano size pillar surface culture dish in this experiment. The diameter size of nano pillar is 120nm to 360nm and we separate the gradient topography as High (280nm-360nm), Middle (200nm-280nm) and Low (120nm-200nm) respectively. hECFCs was derived from human peripheral blood mononuclear cells (hPBMNCs) and cultured with EGM2-MV endothelial medium. Results: Attachment of hECFCs was decreased on the High (280nm-360nm) nano size pillar area. But, proliferation and apoptosis of hECFCs on the nano size pillar surface has no significant difference with hECFCs on the flat pattern. However, single cell morphology of hECFCs on the nano size pillar surface was distinct compared with hECFCs on the flat pattern. Finally, gene expression level of ROCK, Rho and Integrin family has changed on the nano size pillar surface. Conclusion: In this study we find that biophysical cues from nano size pillar surface can affect single cell morphology of hECFC and gene expression level. Further, through these several results we can know that ROCK family are related with biophysical cues from nanotopography and nano pillar diameter size can affect the optimal culture condition for hECFC.