Description of Stegelletina lingulata sp. n. (Nematoda, Rhabditida, Cephalobidae) from xeric environments in Iran

Zootaxa ◽  
2017 ◽  
Vol 4358 (3) ◽  
pp. 462
Author(s):  
JOAQUÍN ABOLAFIA ◽  
EBRAHIM SHOKOOHI

A new species belonging to the genus Stegelletina, S. lingulata sp. n., is described from xeric natural areas from Iran. It is characterized by having small body (0.51–0.69 mm in females and 0.45–0.64 mm in males), cuticle slightly tessellated especially at anterior end, lateral field with three longitudinal incisures, lips with long acute process at primary axils and three more dentate processes at secondary axils, primary axils with one triangular axillar guard process and secondary axils lacking axillar processes, labial probolae long (9–11 µm) with slender peduncle lacking adaxial expansion and with convergent smooth prongs, stoma, pharynx and reproductive systems cephaloboid, excretory pore at isthmus level, spermatheca 1.1–1.4 times, and post-vulval sac 0.9–1.2 times the corresponding body diameters in length, female rectum 0.6–0.8 times anal body diameter long, female tail conoid (35–47 µm long, c = 14.0–15.6, c' = 2.1–2.7) with fine rounded terminus, male tail conoid, curved ventrad (53–59 µm long, c = 11.0–12.0, c' = 1.9–2.5) with rounded terminus, spicules 31–33 µm long with ventrally straight manubrium and gubernaculum 14–17 µm long. Measurements and illustrations (line drawings, LM and SEM) are provided for this species. In addition, comments on the morphology of the lip region of the species of the genus Stegelletina are included. 


Zootaxa ◽  
2012 ◽  
Vol 3407 (1) ◽  
pp. 61
Author(s):  
NEGIN AMIRZADI ◽  
EBRAHIM SHOKOOHI ◽  
ALI ESKANDARI ◽  
JOAQUÍN ABOLAFIA

A new species of the genus Acrolobus was recovered during a survey of soil nematodes. Acrolobus longigubernaculumsp. n., collected from the rhizosphere of weeds in northeastern Iran, is described and illustrated. It is characterized by abody length of 0.72–0.98 mm in females and 0.73–0.84 mm in males, annulated cuticle, lateral fields with three longitu-dinal incisures, lip region slightly offset from the neck and 8–11μm wide, lips leaf-like, stoma 12–16 µm long, pharyngealcorpus 2.7–3.2 times isthmus length, vulva located slightly posterior to middle of body (V = 61–65), spermatheca 26–46µm long, postuterine sac 27–49 µm long or 0.9–1.3 times the corresponding body diameter, female tail conical with a fine,short mucro (47–58 µm, c = 13.9–18.5, c’ = 2.4–3.2), male tail conical bearing a fine mucro (47–50 µm, c = 14.8–17.5;c’ = 2–2.3), spicules 26–30μm long and ventrally curved, and gubernaculum 12–16 µm long (50% of spicule length). The new taxon is the second species of the genus Acrolobus and is compared to the type species, A. emarginatus.



Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.



Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 709-723 ◽  
Author(s):  
Zhen Wang ◽  
Wim Bert ◽  
Jianfeng Gu ◽  
Marjolein Couvreur ◽  
Hongmei Li

Summary Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μm long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μm long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis.



Zootaxa ◽  
2019 ◽  
Vol 4551 (2) ◽  
pp. 244
Author(s):  
HADI PANAHI ◽  
EBRAHIM SHOKOOHI ◽  
MARIA T VINCIGUERA ◽  
MIRELLA CLAUSI ◽  
PHATU. W. MASHELA

A new species of the genus Paractinolaimus, namely, Paractinolaimus persicus sp. n., is described. It is characterised by body 2.3–2.6 mm long; low, truncate lip region; four massive, acute onchia; stoma walls covered with sparse tiny denticles; odontostyle 25–28 μm long; well developed, didelphic-amphidelphic female reproductive apparatus; vulva a longitudinal oval pore; absence of advulval papillae; female tail 146–224 µm long (c’ = 3.3–5.9), elongated filiform, curved ventrad, and with acute terminus; male tail convex conoid with blunt terminus; and 17–18 nearly contiguous ventromedian supplements. This species was recovered from the rhizosphere of wild grass. Line drawings, LM and SEM photographs and measurements are provided. 



Nematology ◽  
2019 ◽  
Vol 21 (9) ◽  
pp. 957-969 ◽  
Author(s):  
Giulia Torrini ◽  
Agostino Strangi ◽  
Giuseppe Mazza ◽  
Leonardo Marianelli ◽  
Pio Federico Roversi ◽  
...  

Summary A new species of Bursaphelenchus, extracted from unprocessed logs of Milicia excelsa from Cameroon and arriving in the port of Leghorn, Tuscany, Central Italy, is described. Bursaphelenchus irokophilus n. sp. is based upon morphological and molecular characters and is characterised by the two-lined lateral field, mitten-shaped spicule possessing cucullus and well-developed and slightly dorsally curved condylus with narrowly rounded tip, female vulva with side flap, and long, smoothly tapering and strongly ventrally recurved female tail. The new species belongs to the abietinus-group and is similar to, but distinguished from, B. abietinus and B. rainulfi by the position of secretory-excretory pore, longer and slender female tail and some morphometric values. In addition, the phylogeny based on SSU and D2-D3 LSU regions shows that this species belongs to the abietinus-group, the relationship among the species of this group being discussed.



Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Xiaoling Lü ◽  
Xianfeng Chen

Summary Ruehmaphelenchus taedae n. sp., isolated from Loblolly pine logs (Pinus taedae L.) from the USA, is described and figured. It is characterised by a relatively slim body (a = 42 and 43 for males and females, respectively), three lines in the lateral field, male spicules relatively small (12-18 μm) with high and dorsally bent condylus and weakly developed rostrum, bursal flap absent, short tail possessing a long terminal spike ending in a bluntly rounded tip and 8.7-13.3 μm long, vulva positioned at ca 83% of body length, vulval flap absent, vulval lips slightly protruding, post-vulval uterine branch extending for less than half of vulva to anus distance, and female tail conoid, ca 3-4 anal body diam. long, with 13.7-18.5 μm terminal projection. The new species can be separated from all other species of the genus by the male tail possessing a long terminal spike and the more anterior excretory pore. Detailed phylogenetic analysis based on 28S D2-D3 region sequences confirmed the status of this nematode as a new species.



Nematology ◽  
2005 ◽  
Vol 7 (3) ◽  
pp. 469-477
Author(s):  
Tom Bongers ◽  
Oleksandr Holovachov ◽  
Alejandro Esquivel

AbstractTwo species of the genus Deficephalobus, D. mirabilis sp. n. and D. desenderi, are described and illustrated on the basis of material collected in Costa Rica. The new species is characterised by 0.67-1.2 mm long body; bluntly rounded labial region; amphidial aperture one-third of labial region diam. wide; cheilostom barrel-shaped with sclerotised, bar-shaped, cheilorhabdia; gymnostom barrel-shaped with moderately sclerotised lining of gymnostom; stegostom funnel-shaped anteriorly, narrow tubular posteriorly and subdivided into four sets of rhabdia; basal bulb elongate ovoid with weakly developed valvular apparatus; excretory pore opening anterior to nerve ring; ovary tip almost reaching the level of rectum, large spermatheca, postvulval uterine sac shorter than vulval body diameter; female tail elongate conoid and straight, with filiform tail terminus; male tail short conoid with small, ragged, terminal mucro.



Nematology ◽  
2013 ◽  
Vol 15 (3) ◽  
pp. 279-289 ◽  
Author(s):  
Xuan Wang ◽  
Pei Wang ◽  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Hongmei Li

Aphelenchoides xui sp. n. is described and illustrated from imported packaging wood from South Africa intercepted at Ningbo Port, China. The new species has a body length of 564-820 μm (males) and 549-882 μm (females). The cuticle is weakly annulated with four lines in the lateral field. The stylet is 11-13 μm long and has small basal swellings. The excretory pore is located ca one body diam. posterior to the median bulb, or 75-100 μm from the head. Spicules are relatively large (18-23 μm) with apex and rostrum rounded and well developed and the end of the dorsal limb clearly curved ventrad like a hook. The male tail bears six (2 + 2 + 2) caudal papillae. The spermatheca is axial and oblong and contains disc-like sperm. The female tail is conical, terminating in a complicated step-like projection, usually with many tiny nodular protuberances. The new species is morphologically similar to A. arcticus, A. haguei and A. parasaprophilus in Group 2 sensu Shahina, but is distinguished by spicule shape and form of the female tail terminus. Phylogenetic analysis based on SSU and partial LSU sequences revealed that A. xui sp. n. was closest to A. varicaudatus, although some species of Laimaphelenchus and Schistonchus were also included in the same branch.



Zootaxa ◽  
2005 ◽  
Vol 988 (1) ◽  
pp. 1 ◽  
Author(s):  
JIANFENG GU ◽  
JIANCHENG ZHANG ◽  
HELEN BRAASCH ◽  
WOLFGANG BURGERMEISTER

Bursaphelenchus singaporensis sp. n. isolated in China from packaging wood of deciduous trees, imported from Singapore is described and illustrated. This new species clearly belongs to the B. xylophilus group, having males with the typically shaped spicules with a cucullus at their distal extremity, the typical position and number of caudal papillae (three pairs and one single) and the anterior vulval lip of the females developed as a distinct flap. The new species is characterized by a body length of 792 (553–950) µm and 850 (690–961) µm of males and females, respectively, robust body (a= 34 and 31, resp.), 15–16 µm-long stylet, lateral field with four lines, long postuterine branch (averaging 102 µm) and a strongly conoid female tail (c= 20) with a finely rounded, only slightly ventrally-bent terminus, male with very strong spicules (41–48 µm long), distinct rostrum and small cucullus, and a dorso-ventral visible terminal bursa. Bursaphelenchus singaporensis sp. n. is closely related to other species of the B. xylophilus group (B. xylophilus, B. mucronatus, B. kolymensis, B. fraudulentus, B. conicaudatus, B. baujardi and B. luxuriosae) and similar to B. abruptus. The morphological differentiation is mainly based on the shape of the female tail. However, B. singaporensis sp. n. differs from all other species of the B. xylophilus group by larger spicules. The new species can be differentiated from B. abruptus, B. xylophilus, B. mucronatus, B. fraudulentus, B. conicaudatus and B. luxuriosae by means of ITS-RFLP patterns.



Zootaxa ◽  
2018 ◽  
Vol 4399 (2) ◽  
pp. 197 ◽  
Author(s):  
EBRAHIM SHOKOOHI ◽  
FAHIME IRANPOUR ◽  
VLADA PENEVA ◽  
MILKA ELSHISHKA ◽  
HENDRIKA FOURIE ◽  
...  

During a survey of soil nematodes in Iran, a new species of Ditylenchus Filipjev, 1936 was discovered. Ditylenchus sarvarae sp. n. is characterised by its body length (1.0–1.4 mm), lateral field with seven incisures and without areolation, long postuterine sac (1.8–2.8 times the corresponding body diameter) and conical female tail with pointed tip (68–89 μm long, c = 13.7–18.2, c' = 4.2–5.1). Male specimens have conical tails (64–70 μm long, c = 14.5–16.7, c' = 4.0–4.5), spicules (22–26 μm long) and gubernaculum (8–10 μm long). Measurements and illustrations are provided for this new species. A molecular study of the 28S rDNA region of D. sarvarae sp. n. demonstrates that the Iranian species belongs to a separate group compared with the other molecularly characterized species of genus Ditylenchus. 



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