Bursaphelenchus fagi sp. n. (Nematoda: Parasitaphelenchidae), an insect-pathogenic nematode in the Malpighian tubules of the bark beetle, Taphrorychus bicolor (Herbst.) (Coleoptera: Curculionidae, Scolytinae), in European beech, Fagus silvatica L.

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 591-606 ◽  
Author(s):  
Marek Tomalak ◽  
Anna Filipiak
Keyword(s):  
New Species ◽  
Bark Beetle ◽  
Its Region ◽  
European Beech ◽  
Close Relation ◽  
The Body ◽  
Malpighian Tubules ◽  
Molecular Profile ◽  
Fagus Silvatica ◽  
Taphrorychus Bicolor

Bursaphelenchus fagisp. n. is described from the bark of European beech,Fagus silvatica. All propagative stages of the nematode are numerous in larval galleries of the beech bark beetle,Taphrorychus bicolor, while dauer juveniles aggregate in Malpighian tubules of adult beetles. The new species is characterised by the body length of 871 (763-1110) μm in female and 852 (718-992) μm in male, very slender body (a = 56.8 (50.4-67.1) and 64.5 (56.5-73.8) in female and male, respectively), and spicules 15.2 (14.0-17.0) μm long. The extended anterior vulval lip in female, lateral fields with four incisures, and number (7) and arrangement of male caudal papillae may indicate thatB. fagisp. n. is closely related to thexylophilusgroup. It differs from this group by the relatively small, claw-like spicules with narrow capitulum, indistinct condylus in almost continuous line with dorsal lamina, and lack of distinct cucullus at the spicule tip. The close relation ofB. fagisp. n. with thexylophilusgroup has been confirmed by DNA sequencing and phylogenetic analysis of the 28S rDNA region.Bursaphelenchus fagisp. n. most closely resemblesB. tokyoensisandB. idius, but can be separated by differences in the arrangement of the male caudal papillae, a unique feature for each species. The taxonomic separation of the new species is also confirmed by the unique molecular profile of the ITS region (ITS-RFLP). The mass presence of dauer juveniles ofB. fagisp. n. in Malpighian tubules of adultT. bicolorleads to degradation of the tubule cellular epithelium and local expansion of its basement membrane. These changes clearly indicate direct pathogenicity of the nematode to its vector insect. In laboratory rearing,B. fagisp. n. can grow and reproduce onBotryotinia fuckelianacultures.

Description of Bursaphelenchus taphrorychi sp. n. (Nematoda: Parasitaphelenchidae), the second Bursaphelenchus species from larval galleries of the beech bark beetle, Taphrorychus bicolor (Herbst.) (Coleoptera: Curculionidae: Scolytinae), in European beech, Fagus sylvatica L.

Nematology ◽  
2017 ◽  
Vol 19 (10) ◽  
pp. 1217-1235 ◽  
Author(s):  
Marek Tomalak ◽  
Tadeusz Malewski ◽  
Jianfeng Gu ◽  
Zhan Fa-Qiang
Keyword(s):  
New Species ◽  
Fagus Sylvatica ◽  
Bark Beetle ◽  
Tail Length ◽  
Its Region ◽  
European Beech ◽  
The Body ◽  
Molecular Profile ◽  
Morphometric Characters ◽  
Taphrorychus Bicolor

Bursaphelenchus taphrorychi sp. n. is described from the bark of European beech, Fagus sylvatica. All propagative stages of the nematode are numerous in larval galleries of the beech bark beetle, Taphrorychus bicolor, while dauer juveniles are transmitted to new breeding trees under the elytra of adult beetles. The new species is characterised by the body length of 782 (717-858) μm in female and 638 (475-789) μm in male, moderately slender body (a = 35.0 (31.7-36.5) and 35.5 (31.4-37.1) in female and male, respectively), spicules 12.0-16.0 μm long, lateral fields with four incisures (i.e., three bands), and the arrangement of the seven male caudal papillae (i.e., a single precloacal ventromedian papilla (P1), one pair of adcloacal ventrosublateral papillae (P2), one postcloacal pair (P3) located at ca 60% of the tail length, posterior to the cloacal aperture, and one pair (P4) of subventral papillae of a similar size as the previous pair, but with somewhat sunken tips, located near base of bursa). In the number and arrangement of caudal papillae, stout and curved spicules with prominent rostrum and condylus, small vulval flap, body narrowed posterior to vulva, four incisures in the lateral fields, and long post-uterine sac, B. taphrorychi sp. n. shares most of the key morphological characters with members of the sexdentati-group. However, the newly described species is unique amongst Bursaphelenchus species of this group by the combination of shape of female tail, shape of spicules, and some other morphometric characters. The close relation of B. taphrorychi sp. n. with members of the sexdentati-group has been confirmed by DNA sequencing and phylogenetic analysis of the 28S rDNA region. The taxonomic separation of the new species is also confirmed by the unique molecular profile of the ITS region (ITS-RFLP). In laboratory rearing, B. taphrorychi sp. n. can develop and reproduce on Botrytis cinerea cultures.

Bursaphelenchus tiliae sp. n. (Nematoda: Parasitaphelenchidae), a nematode associate of the bark beetle Ernoporus tiliae (Panz.) (Coleoptera: Curculionidae, Scolytinae), in small-leaved lime, Tilia cordata Mill.

Nematology ◽  
2014 ◽  
Vol 16 (10) ◽  
pp. 1181-1196 ◽  
Author(s):  
Marek Tomalak ◽  
Tadeusz Malewski
Keyword(s):  
Bark Beetle ◽  
Its Region ◽  
Close Relation ◽  
The Body ◽  
Malpighian Tubules ◽  
Molecular Profile ◽  
Tilia Cordata ◽  
The Status ◽  
Partial Degradation ◽  
Almost Continuous

Bursaphelenchus tiliae sp. n. is described from the bark of dead branches of the small-leaved lime, Tilia cordata, infested with the lime bark beetle, Ernoporus tiliae. The nematode adults and propagative juveniles were present in larval galleries, while dispersal dauer juveniles colonised the Malpighian tubules of adult beetles. Bursaphelenchus tiliae sp. n. is characterised by the body length of 664 (568-817) μm in female and 622 (515-754) μm in male, moderately slender body (a = 41.0 (34.7-47.9) and 43.1 (37.8-51.9) in female and male, respectively), and spicules 20.0 (18.1-21.8) μm long. The extended anterior vulval lip in female, lateral fields with four incisures, long post-uterine sac, and number (7) and arrangement of male caudal papillae, may indicate that B. tiliae sp. n. is closely related to the xylophilus group. It differs from this group by smaller, arcuate spicules with relatively short rostrum, narrow capitulum (5.2-6.9 μm), and indistinct condylus in an almost continuous line with dorsal lamina. Distal third of spicule dorsal contour is usually almost straight. The spicules have a distinct, disk-like cucullus at the terminus. The status of the new species is confirmed by the unique molecular profile of the ITS region (ITS-RFLP) and its close relation to the xylophilus group by DNA sequencing and phylogenetic analysis of the 28S rDNA region. The presence of B. tiliae sp. n. dauer juveniles in Malpighian tubules of their vector bark beetle, E. tiliae, caused limited pathogenicity expressed as a local expansion of the tubule basement membrane and partial degradation of the cellular epithelium.

Bursaphelenchus masseyi sp. n. (Nematoda: Parasitaphelenchinae) – a nematode associate of the bark beetle, Trypophloeus populi Hopkins (Coleoptera: Curculionidae: Scolytinae), in aspen, Populus tremuloides Michx. affected by sudden aspen decline in Colorado

Nematology ◽  
2013 ◽  
Vol 15 (8) ◽  
pp. 907-921 ◽  
Author(s):  
Marek Tomalak ◽  
James Worrall ◽  
Anna Filipiak
Keyword(s):  
New Species ◽  
Bark Beetle ◽  
Populus Tremuloides ◽  
Its Region ◽  
The Body ◽  
Molecular Profile ◽  
Morphometric Characters ◽  
Cytospora Chrysosperma ◽  
Sudden Aspen Decline

Bursaphelenchus masseyi sp. n. is described from trunks of unhealthy trembling aspen, Populus tremuloides, affected by sudden aspen decline (SAD) in Colorado, USA. All propagative stages of the nematode were present in larval galleries of a bark beetle, Trypophloeus populi. The dauer juveniles occupy the haemocoel of older larvae, pupae and adult beetles. The characteristic morphology of the male spicules with small but distinct cucullus, extended anterior vulval lip in female, lateral fields with four incisures, and number and arrangement of male caudal papillae, indicate that B. masseyi sp. n. is closely related to the xylophilus group. This relation has been confirmed by DNA sequencing and phylogenetic analysis of the 28S rDNA region. The new species is characterised by the body length of 958 (765-1203) μm in female and 874 (691-1122) μm in male, moderately slender body (a = 38.2 (33.5-41.1) and 38.0 (33.1-44.1) in female and male, respectively), and spicules 29.2 (26.2-34.7) μm long with a small cucullus (1.3-1.5 μm in diam.) at their tips. Bursaphelenchus masseyi sp. n. can be separated from other species in the xylophilus group by the morphology of spicules which have a short capitulum and unique rostrum that is pointed somewhat anteriorly, relatively thick vulval flap, which is straight, parallel to the body long axis and bent towards the body wall at its distal end, and other morphological and morphometric characters. The new species most closely resembles B. trypophloei, but differs by the morphology of spicules (short but distinct condylus vs condylus in a continuous line with dorsal lamina, and smaller cucullus). The taxonomic separation of the new species is also confirmed by the unique molecular profile of the ITS region (ITS-RFLP). Diallelic cross-breeding in vitro revealed also that B. masseyi sp. n. and B. trypophloei are reproductively incompatible. The new species showed poor ability to develop and reproduce on Botrytis cinerea cultures, although it grew vigorously on laboratory cultures of Polish and Colorado isolates of Cytospora chrysosperma, the fungus naturally associated with galleries of the nematode vector, T. populi.

Description of Bursaphelenchus piceae sp. n. (Nematoda: Parasitaphelenchinae) from larval galleries of the six-toothed spruce bark beetle, Pityogenes chalcographus (L.) (Coleoptera: Curculionidae: Scolytinae), in Norway spruce, Picea abies (L.) Karsten

Nematology ◽  
2015 ◽  
Vol 17 (10) ◽  
pp. 1165-1183 ◽  
Author(s):  
Marek Tomalak ◽  
Jan J. Pomorski
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Picea Abies ◽  
Norway Spruce ◽  
Bark Beetle ◽  
The Body ◽  
Malpighian Tubules ◽  
Pityogenes Chalcographus ◽  
Spruce Bark Beetle ◽  
Spruce Bark

Bursaphelenchus piceae sp. n. is described from the bark of Norway spruce, Picea abies. Adults and propagative juveniles of the nematode are found in larval galleries of the six-toothed spruce bark beetle, Pityogenes chalcographus, while its dauer juveniles aggregate in Malpighian tubules of older larvae, pupae and adult beetles. The new species is characterised by the body length of 719 (530-945) μm in female and 632 (474-808) μm in male, its moderately slender body (a = 41.4 (35.4-50.2) and 42.6 (35.1-47.2) in female and male, respectively), and small spicules (16.0 (14.0-18.3) μm along arc and 13.7 (12.2-15.5) μm along chord). The extended anterior vulval lip in female, lateral fields with four incisures, long post-uterine sac extending for 67-91% of vulva-anus distance, and number (7) and arrangement of male caudal papillae suggest that B. piceae sp. n. is closely related to the xylophilus-group yet it differs by the relatively small, claw-like spicules with no cucullus at the tip. The close relation of B. piceae sp. n. to the xylophilus-group has been confirmed by DNA sequencing and phylogenetic analysis. Morphologically B. piceae sp. n. most closely resembles B. tokyoensis and B. fagi but can be separated from both by the unique shape of the spicules. The taxonomic separation of the new species is also confirmed by the unique molecular profile of the ITS region (ITS-RFLP). The presence of dauer juveniles of B. piceae sp. n. in Malpighian tubules of adult P. chalcographus may lead to extensive damage to this organ as shown by local expansion of the tubule basement membrane and degradation of its cellular epithelium. Detailed phylogenetic analysis revealed that B. piceae sp. n. together with five other bark beetle-associated Bursaphelenchus species, namely B. trypophloei, B. masseyi, B. tiliae, B. tokyoensis and B. fagi, constitutes a small phylogenetic clade that is most closely located to, but separate from, the xylophilus-group.

Bursaphelenchus trypophloei sp. n. (Nematoda: Parasitaphelenchinae) – an associate of the bark beetle, Trypophloeus asperatus (Gyll.) (Coleoptera: Curculionidae, Scolytinae), in aspen, Populus tremula L.

Nematology ◽  
2011 ◽  
Vol 13 (5) ◽  
pp. 619-636 ◽  
Author(s):  
Marek Tomalak
Keyword(s):  
Bark Beetle ◽  
Its Region ◽  
The Body ◽  
Populus Tremula ◽  
Molecular Profile ◽  
Morphometric Characters ◽  
Cytospora Chrysosperma ◽  
Reproductive Incompatibility ◽  
Large Populations

Abstract Bursaphelenchus trypophloei sp. n. is described from weakened and dead twigs of living or recently fallen aspen trees, Populus tremula, in Poland. All propagative stages of the nematode were present in larval galleries of a bark beetle, Trypophloeus asperatus, and its dauer juveniles could be found in the haemocoel of older larvae, pupae and adult beetles. Characteristic morphology of the male spicules with distinct cucullus, extended anterior vulval lip, lateral fields with four incisures, and number and arrangement of male caudal papillae indicate that B. trypophloei sp. n. is closely related to the xylophilus group. This relation has been further confirmed by DNA sequencing and phylogenetic analysis of the 28S and ITS-1 rDNA region. Bursaphelenchus trypophloei sp. n. can be separated from other species in the xylophilus group by the morphology of spicules which have a shorter capitulum and unique rostrum which is pointed somewhat anteriorly, relatively thick vulval flap, which is straight, parallel to the body long axis or bent towards the body wall at its distal end, and other morphological and morphometric characters, i.e., shape of female tail, position of excretory pore at or posterior to nerve ring, spicule length of 25.7 (23.1-28.0) μm (as measured along arc) and shape, moderate length, i.e., L = 702 (603-946) μm in females and L = 679 (543-828) μm in males, and slender body (a = 39.2 (33.0-45.2) and 40.9 (32.0-46.4) in female and male, respectively). The taxonomic separation of the new species is also confirmed by the unique molecular profile of the ITS region (ITS-RFLP). Full reproductive incompatibility of B. trypophloei sp. n. with B. xylophilus, B. mucronatus, B. fraudulentus, B. populi and B. doui has been demonstrated in in vitro diallelic cross-breeding. The newly described nematode revealed limited ability to develop and reproduce on Botrytis cinerea cultures although it produced large populations on laboratory cultures of Cytospora chrysosperma – a fungus naturally associated with galleries of the nematode vector, T. asperatus.

Taxonomic description and 3D modelling of a new species of myzostomid (Annelida, Myzostomida) associated with black corals from Madagascar

Zootaxa ◽  
2017 ◽  
Vol 4244 (2) ◽  
pp. 277 ◽  
Author(s):  
LUCAS TERRANA ◽  
IGOR EECKHAUT
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Three Dimensional ◽  
Close Relation ◽  
Ventral Side ◽  
Middle Part ◽  
The Body ◽  
Taxonomic Description ◽  
Black Corals ◽  
Organ Systems

Eenymeenymyzostoma nigrocorallium n. sp. is the first species of myzostomid worm associated with black corals to be described. Endoparasitic specimens of E. nigrocorallium were found associated with three species of antipatharians on the Great Reef of Toliara. Individuals inhabit the gastrovascular ducts of their hosts and evidence of infestation is, most of the time, not visible externally. Phylogenetic analyses based on 18S rDNA, 16S rDNA and COI data indicate a close relation to Eenymeenymyzostoma cirripedium, the only other species of the genus. The morphology of E. nigrocorallium is very unusual compared to that of the more conventional E. cirripedium. The new species has five pairs of extremely reduced parapodia located on the body margin and no introvert, cirri or lateral organs. Individuals are hermaphroditic, with the male and female gonads both being located dorsally in the trunk. It also has a highly developed parenchymo-muscular layer on the ventral side, and the digestive system lies in the middle part of the trunk. A three-dimensional digital model of this worm’s body plan has been constructed whereby the external morphology and in toto views of the observed organ systems (nervous, digestive and reproductive) can be viewed on-screen: http://doi.org/10.13140/RG.2.2.17911.21923. 

Description of Bursaphelenchus populi sp. n. (Nematoda: Parasitaphelenchidae), a new member of the xylophilus group from aspen, Populus tremula L., in Europe

Nematology ◽  
2010 ◽  
Vol 12 (3) ◽  
pp. 399-416 ◽  
Author(s):  
Marek Tomalak ◽  
Anna Filipiak
Keyword(s):  
New Species ◽  
Its Region ◽  
Populus Tremula ◽  
Molecular Profile ◽  
Morphometric Characters ◽  
Female Tail ◽  
Reproductive Incompatibility ◽  
The Status ◽  
Vector Insect

AbstractBursaphelenchus populi sp. n. is described from dying and dead aspen trees, Populus tremula, in Poland. The nematode was found in trees infested with a long-horn beetle, Saperda perforata, and is vectored in the insect haemocoel. The characteristic morphology of male spicules, extended anterior vulval lip, lateral fields with four incisures, and number and arrangement of male caudal papillae, place B. populi sp. n. in the xylophilus group. Bursaphelenchus populi sp. n. can be separated from all other species in that group by the distinctive vulval flap, which is always bent with its distal half sunken in a conspicuous, sharp depression posterior to the vulva, and other morphological and morphometric characters, i.e., female tail shape, excretory pore position, spicule length (32.1 (25.7-37.0) μm (as measured along arc) and shape, and a relatively long (i.e., L,= 1020 (909-1111) μm in females), and L = 850 (756-1055) μm in males), and slender body (a = 45.4 (40.2-52.4) and 42.0 (36.1-49.3) in female and male, respectively). The status of the new species is confirmed by the unique molecular profile of the ITS region (ITS-RFLP). DNA sequencing and phylogenetic analysis of 28S rDNA region placed the new species close to B. fraudulentus in the xylophilus group. In vitro cross-breeding of the new nematode with B. xylophilus, B. mucronatus, B. fraudulentus and B. doui revealed full reproductive incompatibility between these species. In laboratory experiments on 2-year-old seedlings B. populi sp. n. was specific to aspen. It did not develop on pine or oak and reproduced only in weakened or dead aspen seedlings. No pathogenicity to the tree host or vector insect was observed. In Botrytis cinerea PDA cultures, body dimensions were significantly altered in both sexes and female tail morphology differed when compared to individuals extracted from aspen wood.

Deladenus prorsus n. sp. (Nematoda: Neotylenchidae) in dunnage wood from Malaysia intercepted in Ningbo, China

Nematology ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. 379-388 ◽  
Author(s):  
Qing Yu ◽  
Jianfeng Gu ◽  
Weimin Ye
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Its Region ◽  
Diagnostic Value ◽  
The Body ◽  
Wood Material ◽  
Anterior Position ◽  
Blue Stain ◽  
Males And Females ◽  
Wood Boring

Deladenus prorsus n. sp., isolated from dunnage wood originating from Malaysia and intercepted in Ningbo port, P.R. China, is described and illustrated. The dunnage wood material appeared to have holes resembling the typical exit holes of wood-boring insects and have associated blue stain. The new species is characterised by the presence of both the mycophagous and infective forms (Deladenus consists of mycetophagous-only forms and those with known female dimorphism), the body length of 814 (670-1147) and 898 (812-979) μm for mycetophagous males and females, respectively, and 1129 (1114-1352) μm for the infective female, and by the very anterior position of the excretory pore which is situated at 30.7 (24.4-33.0), 30.6 (25.4-35.6) and 37.9 (27.3-39.7) μm for the mycetophagous males and females and infective females, respectively, and the distance that the excretory pore is located anterior to the hemizonid which is 82 (77-90), 74 (54-92) and 86 (64-106) μm for the mycetophagous males and females and infective females, respectively. The diagnostic value of the excretory pore and the hemizonid of the species of the genus are discussed. The ribosomal DNA ITS region was sequenced and analysed.

Heterorhabditis mexicana n. sp. (Rhabditida: Heterorhabditidae) from Tamaulipas, Mexico, and morphological studies of the bursa of Heterorhabditis spp.

Nematology ◽  
2004 ◽  
Vol 6 (2) ◽  
pp. 231-244 ◽  
Author(s):  
Khuong Nguyen ◽  
David Sharpiro-Ilan ◽  
Robin Stuart ◽  
Clay McCoy ◽  
Rosalind James ◽  
...  
Keyword(s):  
New Species ◽  
Its Region ◽  
Molecular Data ◽  
The Body ◽  
Sister Taxon ◽  
Infective Juvenile ◽  
Body Width ◽  
Morphological Studies ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract A new species of nematode in the genus Heterorhabditis was found in the northern part of the state of Tamaulipas, Mexico. Morphological and molecular data indicate that this nematode is a new species. The new species is described as Heterorhabditis mexicana n. sp. and is a sister taxon to H. indica. Heterorhabditis mexicana n. sp. is morphologically similar to H. bacteriophora, H. brevicaudis and H. indica and can be distinguished from these species mainly by male and female characters. Of the examined specimens of H. mexicana n. sp., 70% of males have eight pairs of bursal papillae, compared to nine in all other species. The ratio of the gubernaculum to spicule length (GS ratio) is higher than that of H. bacteriophora, H. brevicaudis and H. indica and the length of the spicule relative to anal body width (SW) is lower than all other species. For females, the vulval form of the new species is quite different from that of those species with a similar morphology (i. e ., H. bacteriophora and H. indica) and more closely resembles that of H. zealandica. The new species can also be distinguished from H. megidis, H. zealandica and H. marelatus by the body length, pharynx length of the infective juvenile and D% (distance from anterior end/pharynx length × 100), GS and SW ratios of males. In the ITS region of the rDNA tandem repeating unit, H. mexicana n. sp. has evolved 13 autapomorphic nucleotide character states, differing from its sister taxon H. indica at 113 aligned positions. The morphological and molecular data are sufficient to identify cladogenesis and delimit H. mexicana n. sp. as evolving independently from the other members of the genus.

Prothallonema tomici n. sp. (Tylenchida: Sphaerulariidae) parasitising Tomicus piniperda (Coleoptera: Curculionidae: Scolytinae) in Bulgaria

Nematology ◽  
2011 ◽  
Vol 13 (6) ◽  
pp. 741-746 ◽  
Author(s):  
Daniela Pilarska ◽  
Danail Takov ◽  
Sevdan Nedelchev
Keyword(s):  
New Species ◽  
Bark Beetle ◽  
Reproductive System ◽  
Body Cavity ◽  
The Body ◽  
Tomicus Piniperda ◽  
Anterior Position ◽  
Two Generations ◽  
Short Body

AbstractProthallonema tomici n. sp. (Tylenchida: Sphaerulariidae) is described as a parasite of the bark beetle Tomicus piniperda (Linnaeus) (Coleoptera: Curculionidae: Scolytidae). The new species is characterised by the relatively short body (0.3-0.5 mm) and short prolapsed uterus ((0.16-0.44) × (0.065-0.15) mm), relatively anterior position of the vulva (83.8-85.5%), stylet with fine asymmetrical basal knobs, hemizonid and hemizonion, four incisures, subterminal phasmids and tail terminus always curved dorsad. The entomoparasitic and mycetophagous generation were observed in the body cavity of T. piniperda. Comparing the two generations of J4 showed that the mycetophagous generation had a longer and more robust body, shorter pharynx and tail, longer reproductive system and vulva located further posteriorly. It can be concluded that, in the haemocoel, the entomoparasitic generation can develop through all stages whilst the mycetophagous generation develops to the J4 and then dies.

Sign in / Sign up

Export Citation Format

Share Document