Description of Ektaphelenchoides caspiensis n. sp. (Nematoda: Ektaphelenchinae) from Iran

Nematology ◽  
2015 ◽  
Vol 17 (2) ◽  
pp. 169-177 ◽  
Author(s):  
Farzad Aliramaji ◽  
Ebrahim Pourjam ◽  
Akbar Karegar ◽  
Mohammad Reza Atighi ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Excretory Pore ◽  
Large Subunit ◽  
Subunit Gene ◽  
Lateral Field ◽  
Molecular Analyses ◽  
Tail Shape ◽  
Anterior Body ◽  
Molecular Characters

Ektaphelenchoides caspiensis n. sp. is described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by its continuous lip region, obscure lateral field, females with body length of 499-572 μm, 9-10 μm long stylet, excretory pore posterior to base of metacorpus at 62-72 μm from anterior body end, short post-uterine sac ca 0.5 times corresponding body diam. or 4-9 μm long, and posterior body end (i.e., including ‘tail’) very long and filiform. Males are common and have 7-8 μm long spicules, five copulatory papillae and a long (109-140 μm) conoid tail with very long filiform terminus. Based on morphological and molecular characters, it comes close to five known species of the genus, namely: E. attenuata, E. fuchsi, E. kelardashtensis, E. musae and E. ruehmi. It differs from the closest species, E. kelardashtensis, by the shorter female stylet (9-10 vs 13-16 μm), more anterior vulva (V = 51.7-58.1 vs 61.5-68.0), more posterior excretory pore (62-72 vs 55-66 μm from anterior end), very long posterior body (i.e., including ‘tail’) in female, and male tail shape (conoid with very long filiform terminus vs conoid, sharply pointed). Comparisons with other close species of the genus are also discussed. Molecular analyses were performed using 732 bp of the partial ribosomal RNA large subunit gene (D2-D3 of LSU) using Bayesian inference (BI) and maximum likelihood (ML) methods and showed that the new species formed a clade with one species of the genus (E. fuchsi) and Devibursaphelenchus lini.

Description of Ektaphelenchus berbericus n. sp. (Rhabditida: Ektaphelenchinae) from eastern Iran

Nematology ◽  
2016 ◽  
Vol 18 (9) ◽  
pp. 1063-1077 ◽  
Author(s):  
Somaye Alvani ◽  
Esmat Mahdikhani-Moghadam ◽  
Robin M. Giblin-Davis ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Slight Variation ◽  
Internal Transcribed Spacer Region ◽  
Lateral Field ◽  
Ribosomal Rna Gene ◽  
Eastern Iran

Ektaphelenchus berbericus n. sp. was recovered from soil samples collected in eastern Iran and is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having female body 512-691 μm long, lip region separated from rest of body by a shallow depression, lips separated and equally sized, 19-22 μm long stylet with wide lumen and lacking knobs or swellings at its base, cuticle with fine, but distinct transverse annuli and three lines in lateral field, excretory pore located at base of metacorpus (with slight variation in position), reproductive system monodelphic-prodelphic with spheroid and fine sperm cells inside spermatheca in some individuals and short post-vulval uterine sac (PUS), rectum and anus vestigial (invisible in few individuals), conical posterior body end (tail), narrowing at mid-point between anus and rounded tail tip, and males lacking. The new species is typologically similar to species belonging to four genera: Devibursaphelenchus, Ektaphelenchoides, Ektaphelenchus and Seinura, by a combination of morphological characters, e.g., gross morphology of stylet (lacking knobs or swellings at base), morphology of posterior body end (having short conical tail), PUS length, and having a vestigial anus and rectum in most individuals. In molecular phylogenetic analyses using sequences of the partial small subunit of the ribosomal RNA gene (SSU) and the D2-D3 expansion segments of the large subunit (LSU) ribosomal RNA gene, the new species is close to the Ektaphelenchoides/Cryptaphelenchus clade in an inferred SSU tree, and formed a clade with Ektaphelenchoides and Devibursaphelenchus spp. in a D2-D3 LSU tree. Further phylogenetic analyses using full length sequences of the internal transcribed spacer region (ITS) (= ITS1 + 5.8S + ITS2) corroborated results from the SSU and D2-D3 LSU trees, and the multilocus analyses using the combined SSU and LSU data placed the new species in a robustly supported clade with Ektaphelenchoides poinari and Devibursaphelenchus lini.

Description of Bursaphelenchus irokophilus n. sp. (Nematoda: Aphelenchoididae) isolated from Milicia excelsa (Welw.) C.C. Berg wood imported into Italy from Cameroon

Nematology ◽  
2019 ◽  
Vol 21 (9) ◽  
pp. 957-969 ◽  
Author(s):  
Giulia Torrini ◽  
Agostino Strangi ◽  
Giuseppe Mazza ◽  
Leonardo Marianelli ◽  
Pio Federico Roversi ◽  
...  
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Central Italy ◽  
Lateral Field ◽  
Female Tail ◽  
Milicia Excelsa ◽  
The Relationship ◽  
A New Species ◽  
Molecular Characters

Summary A new species of Bursaphelenchus, extracted from unprocessed logs of Milicia excelsa from Cameroon and arriving in the port of Leghorn, Tuscany, Central Italy, is described. Bursaphelenchus irokophilus n. sp. is based upon morphological and molecular characters and is characterised by the two-lined lateral field, mitten-shaped spicule possessing cucullus and well-developed and slightly dorsally curved condylus with narrowly rounded tip, female vulva with side flap, and long, smoothly tapering and strongly ventrally recurved female tail. The new species belongs to the abietinus-group and is similar to, but distinguished from, B. abietinus and B. rainulfi by the position of secretory-excretory pore, longer and slender female tail and some morphometric values. In addition, the phylogeny based on SSU and D2-D3 LSU regions shows that this species belongs to the abietinus-group, the relationship among the species of this group being discussed.

Bursaphelenchus parantoniae n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from Belgium

Nematology ◽  
2015 ◽  
Vol 17 (10) ◽  
pp. 1141-1152 ◽  
Author(s):  
Munawar Maria ◽  
Yiwu Fang ◽  
Jie He ◽  
Jianfeng Gu ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Characters

Bursaphelenchus parantoniaen. sp. isolated in Ningbo, China, from packaging wood made ofPinussp. imported from Belgium, is described. It is characterised by a slim body (a = 32.6-51), lateral field with two lines, excretory pore locatedcaone metacorpal length posterior to median bulb, spicules stout and arcuate with triangular rostrum and flattened cucullus, and seven caudal papillae arranged as a single midventral precloacal papilla, one adcloacal pair and two postcloacal pairs. The vagina is inclined anteriorly, a vulval flap is present, the post-uterine branch extends for more than half of the vulva-anus distance, and the female tail is conical with a hyaline region and bluntly rounded terminus. Phylogenetic analyses showed thatB. parantoniaen. sp. clusters with thehylobianum-group and appears close toB. antoniae,B. chengi,B. hylobianumandB. niphades. The new species is distinguished from these species by morphology, ITS-RFLP patterns and molecular characters.

scholarly journals Two new calcicolous caloplacoid lichens from South Korea, with a taxonomic key to the species of Huriella and Squamulea

MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 35-55
Author(s):  
Beeyoung Gun Lee ◽  
Jae-Seoun Hur
Keyword(s):  
New Species ◽  
South Korea ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Small Subunit ◽  
Similar Species ◽  
Molecular Analyses ◽  
Tip Cells ◽  
Large Subunit Ribosomal Rna

Pyrenodesmia rugosa Lee & Hur and Huriella aeruginosa Lee & Hur are described as new lichen-forming fungi from a calcareous mountain of South Korea. Pyrenodesmia rugosa is distinguishable from Pyrenodesmia micromontana (Frolov, Wilk & Vondrák) Hafellner & Türk, the most similar species, by thicker thallus, rugose areoles, larger apothecia, shorter hymenium, shorter hypothecium and narrower tip cells of paraphyses. Huriella aeruginosa, the second new species, differs from ‘Squamulea’ chelonia Bungartz & Søchting by dark greenish-grey to grey thallus without pruina, gold to yellow-brown epihymenium, larger ascospores and thallus K– and KC– reaction. Molecular analyses employing internal transcribed spacer (ITS), mitochondrial small subunit (mtSSU) and nuclear large subunit ribosomal RNA (LSU) sequences strongly support the two caloplacoid species to be distinct in their genera. A surrogate key is provided to assist in the identification of all 20 taxa in Huriella and Squamulea.

Two new species of Paurodontella Husain & Khan, 1968 (Nematoda: Sphaerulariidae) associated with wheat and a diagnostic compendium to the genus

Nematology ◽  
2010 ◽  
Vol 12 (2) ◽  
pp. 181-192 ◽  
Author(s):  
Zafar A. Handoo ◽  
Erum Y. Iqbal ◽  
Nasira Kazi ◽  
Shahina Fayyaz
Keyword(s):  
Triticum Aestivum ◽  
New Species ◽  
Excretory Pore ◽  
Triticum Aestivum L ◽  
Slender Body ◽  
Valid Species ◽  
Lateral Field ◽  
North West ◽  
North West Frontier Province ◽  
Two New Species

Abstract An identification key to ten valid species of Paurodontella is given. A compendium of the most important diagnostic characters with illustrations of each species is included as a practical alternative and supplement to the key. The diagnosis of Paurodontella is emended and a list of all valid species of the genus is given. Two new species (Paurodontella myceliophaga n. sp. and P. balochistanica n. sp.) collected around the roots of wheat (Triticum aestivum L.) from North-West Frontier Province and Balochistan, Pakistan, are described and illustrated. Paurodontella myceliophaga n. sp. is characterised by having a short post-uterine sac, lateral field with four incisures, excretory pore at the base of the pharynx and fusiform median bulb, whereas P. balochistanica n. sp. has a longer and very slender body with posterior vulva, lateral field with four incisures and short mucronate tail. Because these species are limited in distribution, their importance is not known.

scholarly journals Morphological and molecular identification of a new species of Atraporiella (Polyporales, Basidiomycota) in China

Phytotaxa ◽  
2017 ◽  
Vol 332 (1) ◽  
pp. 31 ◽  
Author(s):  
ZI-QIANG WU ◽  
SHAN SHEN ◽  
KAI-YUE LUO ◽  
ZHENG-HUI WANG ◽  
CHANG-LIN ZHAO
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Molecular Data ◽  
Fungal Species ◽  
Monophyletic Lineage ◽  
Inference Methods ◽  
A New Species ◽  
Molecular Characters

A new poroid wood-inhabiting fungal species, Atraporiella yunnanensis sp. nov., is proposed based on morphological and molecular characters. The species is characterized by cream pore surface when dry, which is easy to separate from substrate and very rapidly stained dark brown to black when bruised; hyphal system monomitic with generative hyphae hyaline to pale brown, thin-walled, unbranched, interwoven; slightly allantoid basidiospores, 2.2–3 × 0.8–1.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Atraporiella yunnanensis belonged to the residual polyporoid clade, formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and was closely related to A. neotropica, and then grouped with other related genera: Antrodiella, Pouzaroporia, Steccherinum and Xanthoporus. Both morphological and molecular characters confirmed the placement of the new species in Atraporiella.

Morphological and molecular characterisation of Helicotylenchus ciceri n. sp. and H. scoticus Boag & Jairajpuri, 1985 (Nematoda: Hoplolaimidae) from Iran

Nematology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 611-626
Author(s):  
Fariba Mohammadi Zameleh ◽  
Akbar Karegar ◽  
Reza Ghaderi ◽  
Abbas Mokaram Hesar
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Rrna Genes ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Closely Related Species ◽  
Partial 18S ◽  
Molecular Characters

Summary Helicotylenchus ciceri n. sp. and H. scoticus are described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by a conical and truncated lip region with five or six distinct annuli, stylet 32-37 μm long with anteriorly concave knobs, secretory-excretory pore posterior to the pharyngo-intestinal valve, dorsally convex-conoid tail with a terminal projection, phasmids 14 (7-20) annuli anterior to the level of anus, empty spermatheca and absence of males. Intraspecific variation of 16 populations of H. scoticus, collected from chickpea and lentil fields in Kermanshah province, western Iran, is discussed. The results of the phylogenetic analyses based on the sequences of the partial 18S rRNA, D2-D3 expansion segments of 28S rRNA and ITS rRNA genes are provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related species.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Ceriporia albomellea (Phanerochaetaceae, Basidiomycota), a new species from tropical China based on morphological and molecular evidences

Phytotaxa ◽  
2017 ◽  
Vol 298 (1) ◽  
pp. 20 ◽  
Author(s):  
YUAN YUAN ◽  
XIAO-HONG JI ◽  
FANG WU ◽  
JIA-JIA CHEN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Its Regions ◽  
Tropical China ◽  
A New Species

A new polypore, Ceriporia albomellea, collected from tropical China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 µm. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions supported C. albomellea as a distinctive species belonging to Ceriporia.

Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Molecular Characteristics ◽  
Lsu Rdna ◽  
Northern Iran ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 

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