Two new calcicolous caloplacoid lichens from South Korea, with a taxonomic key to the species of Huriella and Squamulea

Lecanora parasymmicta Lee & Hur and Protoparmeliopsis crystalliniformis Lee & Hur are described as new lichen species to science from the forested wetlands in southern South Korea. Molecular analyses employing internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences strongly support the two lecanoroid species to be distinct in their genera. Lecanora parasymmicta is included in the Lecanora symmicta group. It is morphologically distinguished from Lecanora symmicta (Ach.) Ach., its most similar species, by areolate-rimose thallus, blackish hypothallus, larger apothecia, absence of thalline excipulum from the beginning, narrower paraphyses, larger ascospores, smaller pycnoconidia, and the presence of placodiolic acid. The second new species Protoparmeliopsis crystalliniformis is included in a clade with Protoparmeliopsis bipruinosa (Fink) S.Y. Kondr. and P. nashii (B.D. Ryan) S.Y. Kondr., differs from Protoparmeliopsis ertzii Bungartz & Elix, its most morphologically similar species, by whitish thallus, flat to concave and paler disc, longer ascospores, thallus K+ yellow reaction, presence of atranorin and rhizocarpic acid, and the substrate preference to sandstone or basalt. A key is provided to assist in the identification of Protoparmeliopsis species in Korea.

Download Full-text

A new lichenized fungus, Lecanora baekdudaeganensis, from South Korea, with a taxonomic key for Korean Lecanora species

MycoKeys ◽

10.3897/mycokeys.70.51569 ◽

2020 ◽

Vol 70 ◽

pp. 39-58

Author(s):

Beeyoung Gun Lee ◽

Jae-Seoun Hur

Keyword(s):

New Species ◽

South Korea ◽

Internal Transcribed Spacer ◽

Distinct Species ◽

Small Subunit ◽

Oil Droplets ◽

Molecular Analyses ◽

Taxonomic Key ◽

Lichenized Fungus

Lecanora baekdudaeganensis Lee & Hur is described as a new lichenized fungus from Baekdudaegan Mountains, South Korea. The new species is classified into the Lecanora subfusca group – allophana type and distinguishable from Lecanora imshaugii Brodo by a darker thallus, brownish disc, K–insoluble granules on the surface of the epihymenium, shorter hypothecium, and the presence of oil droplets in the apothecial section. Molecular analyses employing internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences strongly support Lecanora baekdudaeganensis as a distinct species in the genus Lecanora. A surrogate key is provided to assist in the identification of all 52 taxa in the genus Lecanora of Korea.

Download Full-text

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽

10.11646/phytotaxa.409.4.1 ◽

2019 ◽

Vol 409 (4) ◽

pp. 189-201 ◽

Cited By ~ 1

Author(s):

MALKA SABA ◽

DANNY HAELEWATERS ◽

MUHAMMAD FIAZ ◽

ABDUL NASIR KHALID ◽

DONALD H. PFISTER

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Large Subunit ◽

Pine Forests ◽

Evolutionary Relationships ◽

Internal Transcribed Spacer Region ◽

Bayesian Inferences ◽

A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Download Full-text

Ceriporia albomellea (Phanerochaetaceae, Basidiomycota), a new species from tropical China based on morphological and molecular evidences

Phytotaxa ◽

10.11646/phytotaxa.298.1.2 ◽

2017 ◽

Vol 298 (1) ◽

pp. 20 ◽

Cited By ~ 2

Author(s):

YUAN YUAN ◽

XIAO-HONG JI ◽

FANG WU ◽

JIA-JIA CHEN

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Morphological Characteristics ◽

Molecular Evidence ◽

Its Regions ◽

Tropical China ◽

A New Species

A new polypore, Ceriporia albomellea, collected from tropical China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 µm. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions supported C. albomellea as a distinctive species belonging to Ceriporia.

Download Full-text

New observations on Miculenchus Andrássy, 1959 (Nematoda: Tylenchidae) with descriptions of two new and one known species from Iran

Nematology ◽

10.1163/15685411-00003267 ◽

2019 ◽

Vol 21 (9) ◽

pp. 937-956 ◽

Cited By ~ 1

Author(s):

Yousef Panahandeh ◽

Ebrahim Pourjam ◽

Sergio Álvarez-Ortega ◽

Farahnaz Jahanshahi Afshar ◽

Majid Pedram

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Large Subunit ◽

Small Subunit ◽

Lsu Rdna ◽

Natural Forests ◽

Electron Microscopic ◽

Pharyngeal Bulb ◽

Ribosomal Rna Gene ◽

Molecular Approaches

Summary During nematological surveys in grasslands and natural forests of north and north-western Iran, three species of Miculenchus, including two new and one known species, were recovered and characterised based upon morphological and molecular approaches. Miculenchus brevisalvus n. sp., the first new species, is mainly characterised by its short females 334-388 μm long and with a short 6.0-7.5 μm long stylet, pyriform to pyriform-elongate pharyngeal bulb, 4-8 μm long post-uterine sac (PUS), offset rounded spermatheca filled with small spheroid sperm, elongate conoid tail 62-83 μm long with a sharp tip, and males with simple cloacal lips. Miculenchus muscus n. sp., the second new species, is characterised by a combination of the following features: body 401-467 μm long, well-developed protuberant labial plate at the anterior end under light microscopy, stylet 7-9 μm long, pyriform pharyngeal bulb, PUS 4-9 μm long, gradually narrowing conical tail 62-74 μm long with a finely pointed or sharp end and bearing several fine bristles at tip, and a male with projecting cloacal lips. Both newly described species were morphologically compared with four currently known species of the genus, viz., M. elegans, M. salmae, M. salvus, and M. tesselatus. Miculenchus salmae was also recovered and reported from Iran for the first time. It is mainly characterised by lacking a PUS and the characteristic vagina shape. Miculenchus muscus n. sp. and M. salmae were both characterised using scanning electron microscopic images, yielding new morphological observations for the genus. All three species are studied for their molecular phylogenetic characters using sequences of near-full length fragments of the small subunit ribosomal RNA gene (SSU rDNA) and the D2-D3 expansion segments of the large subunit ribosomal RNA gene (LSU rDNA D2-D3). In both SSU and LSU phylogenies, all currently sequenced species of Miculenchus formed a monophyletic group with maximal clade support in both Bayesian inference and maximum likelihood analysis.

Download Full-text

Morphological and phylogenetic analyses reveal a new species of Fistulina (Fistulinaceae, Agaricales) from Australia

Phytotaxa ◽

10.11646/phytotaxa.420.3.3 ◽

2019 ◽

Vol 420 (3) ◽

pp. 233-240

Author(s):

YI-FEI SUN ◽

SHUN LIU ◽

BAO-KAI CUI

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Pore Surface ◽

Ribosomal Rna Gene ◽

Hyphal System ◽

Cotton Blue ◽

A New Species

A new species of Fistulina, F. tasmanica sp. nov., is described and illustrated from Tasmania in Australia. Fistulina tasmanica is distinguished by its pileate and fleshy basidiomata with rose pore surface, fibrous context, separated tubes with small pores (6–7 per mm), a monomitic hyphal system with only clamped generative hyphae, subellipsoid to ellipsoid basidiospores (5.4–6 × 3.4–4 μm) which are negative in Melzer’s reagent and cotton blue. Phylogenetic analyses inferred from the internal transcribed spacer regions (ITS) and the large subunit of nuclear ribosomal RNA gene (nLSU) sequences also confirmed that F. tasmanica is a new member within Fistulina.

Download Full-text

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽

10.11646/phytotaxa.401.3.5 ◽

2019 ◽

Vol 401 (3) ◽

pp. 199

Author(s):

MALKA SABA ◽

DANNY HAELEWATERS ◽

MUHAMMAD FIAZ ◽

ABDUL NASIR KHALID ◽

DONALD H. PFISTER

Keyword(s):

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Large Subunit ◽

Pine Forests ◽

Evolutionary Relationships ◽

Internal Transcribed Spacer Region ◽

Bayesian Inferences ◽

A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Download Full-text

Molecular and morphological characterisation of Aphelenchoides kheirii n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus nigra in north-western Iran

Nematology ◽

10.1163/15685411-00003182 ◽

2018 ◽

Vol 20 (9) ◽

pp. 877-888

Author(s):

Behrouz Golhasan ◽

Ramin Heydari ◽

Mehrab Esmaeili ◽

Natsumi Kanzaki

Keyword(s):

New Species ◽

Large Subunit ◽

Pinus Nigra ◽

Small Subunit ◽

Similar Species ◽

Western Iran ◽

Lateral Lines ◽

Pine Trees ◽

Group 2 ◽

North Western

Summary Aphelenchoides kheirii n. sp. was isolated during a survey of nematodes associated with bark samples of pine trees (Pinus nigra) in West Azerbaijan province, north-western Iran. The new species has a body length of 500 (448-520) μm in the female and 442 (402-480) μm in the male. The cuticle is weakly annulated with four lateral lines and the lip region is offset. The stylet is 10.4 (10-11) μm long with small basal swellings and the excretory pore located immediately posterior to base of metacorpus; hemizonid invisible. The post-vulval uterine sac length 37.7 (28-45) μm. Spicules are relatively short (18-20 μm in dorsal limb) and the end of the dorsal limb clearly curved ventrally like a hook. The male tail has usual three pairs of caudal papillae (2 + 2 + 2) and a well-developed mucron. The female tail is conical, terminating in a complicated step-like projection, usually with many tiny nodular protuberances. The new species belongs to the Group 2 category of Aphelenchoides species sensu Shahina in which ten known species among Groups 2 and 4 sensu Shahina, namely: A. arcticus, A. blastophthorus, A. fuchsi, A. parasaprophilus, A. paraxui, A. xui, A. dactylocercus, A. gynotylurus, A. iranicus and A. saprophilus are the most similar species. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rRNA supported the morphological results.

Download Full-text

A hidden break in the 28.0S rRNA from Diphyllobothrium dendriticum

Journal of Helminthology ◽

10.1017/s0022149x00014553 ◽

1992 ◽

Vol 66 (3) ◽

pp. 193-197 ◽

Cited By ~ 3

Author(s):

K. A. Karlstedt ◽

G. I. L. Paatero ◽

J.-H. Mäkelä ◽

B.-J. Wikgren

Keyword(s):

Ribosomal Rna ◽

Northern Blot ◽

Gel Electrophoresis ◽

Large Subunit ◽

Small Subunit ◽

Agarose Gel ◽

Oligonucleotide Probes ◽

Agarose Gel Electrophoresis ◽

Diphyllobothrium Dendriticum ◽

Large Subunit Ribosomal Rna

ABSTRACTNondenatured and denatured total RNA from the tapeworm Diphyllobothrium dendriticum (Cestoda) was analysed by agarose gel electrophoresis. It was found that the large subunit ribosomal RNA (lrRNA) is 28.0S and the small subunit ribosomal RNA (srRNA) is 19.5S. Following denaturation the 28.0S rRNA was disrupted into a 19.5S subfragment and a 20.7S subfragment due to the presence of a centrally located hidden break. By hybridization of Northern blot membranes with oligonucleotide probes specific for the 5′- and 3′-ends of the lrRNA respectively, we have shown that the 19.5S sub-fragment is from the 5′-end (the α-subfragment) and the 20.7S subfragment from the 3′-end (the β-subfragment) of the 28.0S rRNA of D. dendriticum.

Download Full-text

Inocybe griseorubida, a new species of Pseudosperma clade from tropical India

Phytotaxa ◽

10.11646/phytotaxa.221.2.6 ◽

2015 ◽

Vol 221 (2) ◽

pp. 166 ◽

Cited By ~ 5

Author(s):

K. P. Deepna Latha ◽

Patinjareveettil Manimohan

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Rna Polymerase Ii ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

Similar Species ◽

Internal Transcribed Spacer Region ◽

Comprehensive Description ◽

Kerala State ◽

A New Species

Inocybe griseorubida sp. nov. is described from Kerala State, India. A comprehensive description, photographs, and comparisons with phenetically similar species are provided. The nuclear ribosomal internal transcribed spacer region (ITS), a portion of the nuclear ribosomal large subunit (nLSU) and a portion of the nuclear second-largest subunit of RNA polymerase II (rpb2) gene of this species were sequenced and analyzed. Phylogenetic analysis of rpb2 sequences confirmed both the novelty of the species and its placement within the Pseudosperma clade.

Download Full-text