Two new species of Phasmarhabditis Andrássy, 1976 (Nematoda: Rhabditidae) associated with land snails in Northwest Caucasus, Russian Federation: description and molecular affiliation

Nematology ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 179-197
Author(s):  
Elena S. Ivanova ◽  
Anna P. Geraskina ◽  
Sergei E. Spiridonov

Summary Two new nematode species of Phasmarhabditis were isolated from land snails in North and West Caucasus, Russian Federation. Phasmarhabditis circassica sp. n. originated from Oxychilus sp. collected by the Nickel settlement in the Adygea Republic while P. clausiliiae sp. n. came from Clausiliidae gen. sp. in the Stavropol district. Phasmarhabditis circassica sp. n. is characterised morphologically by male spicules with a hollow tip, a widely conoid, spicate female tail, ensheathed dauer juveniles 896 (813-982) μm long with a tiny but distinct cuticular apical cap and a long filamentous tail, and exsheathed dauers 773 (670-950) μm long with a tail half as long as in the ensheathed dauers and with a rounded tip. Phasmarhabditis clausiliiae sp. n. is very close to P. circassica sp. n. in having similar morphological traits and is differentiated mainly in having a longer female tail of 94 (79-111) vs 82 (54-125 μm) and the much smaller ratio c of 13.7 (10.3-18.4) vs 22.7 (16.8-27.5) and by its molecular characteristics. The molecular analysis based on partial sequences of LSU rDNA, and ITS rDNA regions has been performed and showed that the closest match for both new species was P. bohemica. Despite the morphological similarity between the species described, significant difference between these two new species was reported both for LSU rDNA and ITS rDNA.

Nematology ◽  
2011 ◽  
Vol 13 (5) ◽  
pp. 561-567
Author(s):  
István Andrássy

AbstractTwo new species of Trischistoma are described from Chile and differ from their relatives, and also from the majority of free-living continental nematodes, by their long and exceedingly slender body. Trischistoma tenuissimum sp. n. is characterised by a 4.0-4.2 mm long body, a = 94-112, presence of long post-vulval uterine sac, female tail 7.6 anal body diam. long, three weak precloacal papillae and 60 μm long spicules. Trischistoma subtilissimum sp. n. is 2.9-3.5 mm long with a = 123-146, has a very long post-vulval uterine sac, a female tail 12-14 anal body diam. long, 2-3 weak precloacal papillae and 38-40 μm long spicules.


Nematology ◽  
2018 ◽  
Vol 20 (3) ◽  
pp. 285-297 ◽  
Author(s):  
Elena Ivanova ◽  
Ksenia Perfilieva ◽  
Sergei Spiridonov

A new nematode species recovered from the laboratory culture ofDrosophila melanogasteris described and illustrated. The mass reproduction ofPanagrellus levitatussp. n. in the fly culture occurred several times and resulted in a significant reduction of the fly population. Nematode outbreaks happened after the introduction ofD. melanogasterto the culture from natural sources. The new species is morphologically similar toP. ulmi. Partial LSU rDNA and SSU rDNA sequences were obtained and subjected to phylogenetic analysis that demonstrated the affinity of the new species withPanagrellussp. ‘MC2014’ from a red palm weevil. For the first time, the dauer juveniles ofPanagrelluswere described.


Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 


Author(s):  
Tingting Yu ◽  
Yong Huang ◽  
Kuidong Xu

Two new species of free-living nematodes discovered from the sediments in the East China Sea are described: Linhystera breviapophysis sp. nov. and L. longiapophysis sp. nov. Both species possess a dorso-caudally directed gubernacular apophysis, which makes them distinctly different from the two already known species of Linhystera. Linhystera breviapophysis is characterized by a gubernacular apophysis about 3.3 μm long, the presence of a crown of cervical setae and a filiform tail. Linhystera longiapophysis is characterized by a prominent gubernacular apophysis about 10 μm long, sparse cervical setae and a long filiform tail. An emended diagnosis of Linhystera and a pictorial dichotomous key to the species are given.


Nematology ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 533-538 ◽  
Author(s):  
Alexander Klerman ◽  
Vladimir Gagarin

AbstractTwo new nematode species of the genus Mesacanthion, M. obscurum sp. n. and M. propinquum sp. n., both isolated from sediment in the Mediterranean Sea off the coast of Israel, are described and illustrated. Mesacanthion obscurum sp. n. is similar to M. diplechma and M. southerni, but differs from the first species by having a narrower labial region, shorter outer labial setae, shorter right spiculum, and shorter distance from supplement to cloacal aperture; and from the second species by a shorter female body, shorter outer labial setae, shorter distance from supplement to cloacal aperture, and the presence of a ventral protuberance on the male tail. Mesacanthion propinquum sp. n. resembles M. southerni and M. obscurum sp. n., but differs from the first species by having a shorter female body, less slender tail, narrower labial region, shorter outer labial setae, shorter distance from supplement to cloacal aperture, and absence of transverse striations on the right spicule; and from M. obscurum sp. n. by the shorter outer labial setae (10-15 vs 37-44 μm), shorter distance from supplement to cloacal aperture (28-31 vs 36-59 μm) and absence of transverse striations on the surface of the right spicule. A key is provided for those Mesacanthion species possessing anisomorphic and anisometric spicules.


2002 ◽  
Vol 48 (5) ◽  
pp. 463-467 ◽  
Author(s):  
Helen S Vishniac

Two new species from Iceland are described on the basis of physiological profiles and sequence data from the D2 region of LSU rDNA: Cryptococcus tephrensis (type ICE99-1ToM Y5, ATCC MYA-1765, CBS 8935, GenBank AF317208) and Cryptococcus heimaeyensis (type ICE99-1ToM Y8, ATCC MYA-1759, CBS 8933, GenBank AF370717). The two new species are identifiable from sequence data and can be distinguished from their closest relative, Cryptococcus victoriae, by their higher maximum temperatures for growth, failure to utilize nitrate as sole nitrogen source, and utilization of cadaverine and ethylamine as sole nitrogen sources. Cryptococcus tephrensis is distinguishable from C. heimaeyensis by failure to grow on saccharate as sole source of carbon and energy.Key words: Iceland, Cryptococcus, soil yeasts.


The Festivus ◽  
2022 ◽  
Vol 54 (1) ◽  
pp. 49-53
Author(s):  
Nguyen Thach

Two new species of the land snails in the genus Amphidromus Albers, 1850 from Indonesia, and Vietnam are described: Amphidromus gisellelieae n. sp. from Indonesia, Amphidromus phamvutuanhae n. sp. They are compared to the closest congeners in this genus.


2018 ◽  
Vol 94 ◽  
Author(s):  
S. Álvarez-Ortega ◽  
S.A. Subbotin ◽  
R. Peña-Santiago

Abstract Two new species of the genus Aporcelinus from the USA are described and illustrated. Aporcelinus floridensis sp. n. is characterized by its 1.12–1.52 mm long body, lip region offset by marked constriction and 14.5–17.0 μm broad with perioral liplets, odontostyle 16.5–20.0 μm at its ventral side and 1.1–1.2 times the lip region diameter, neck 316–395 μm long, pharyngeal expansion occupying 43–48% of total neck length, uterus simple and 33–56 μm long or 0.8–1.2 times the corresponding body diameter, V = 48–54, female tail conical (36–49 μm long, c = 27–41, c’ = 1.2–2.0) with finely rounded terminus and no hyaline portion, and male absent. Aporcelinus paolae sp. n. is characterized by its 1.29–1.80 mm long body, lip region offset by marked constriction and 14–16 μm broad, odontostyle 15–17 μm at its ventral side and 1.0–1.1 times the lip region diameter, neck 314–397 μm long, pharyngeal expansion occupying 43–53% of total neck length, uterus tripartite and 128–164 μm long or 2.6–3.6 times the corresponding body diameter, V = 53–57, female tail conical (30–39 μm long, c = 40–51, c’ = 1.1–1.3) with finely rounded terminus and variably re-curved dorsad, male tail conical (27–36 μm, c = 39–59, c’ = 0.9–1.2), ventrally straight and dorsally convex, spicules 48–54 μm long, and 7–9 irregularly spaced ventromedian supplements lacking hiatus. The analyses of the D2-D3 expansion segments of 28S rRNA (LSU) gene sequences of the two new species confirmed the monophyly of the genus, based upon currently available data, showing a close relationship between the genera Aporcelinus and Makatinus, and justified the placement of Aporcelaimellus, Makatinus and Aporcelinus under the subfamily Aporcelaimellinae.


2020 ◽  
pp. 1-33
Author(s):  
Chirasak Sutcharit ◽  
Ekgachai Jeratthitikul ◽  
Piyoros Tongkerd ◽  
Somsak Panha

Indochina land snails of the family Ariophantidae are in need of thorough systematic revision. Here we comprehensively revise the systematics of the large-shelled, sinistral (counterclockwise) coiling snails from Thailand and Peninsular Malaysia. Molecular phylogeny based on mitochondrial (coi and 16S) and nuclear (28S) gene sequences demonstrates that these sinistral snails are not members of Dyakiidae as previously thought, but instead are more closely related to the genus Hemiplecta in the family Ariophantidae. Comparative morphology also reveals similarity of reproductive organ features (globular gametolytic organ, well-developed dart apparatus, and lack of amatorial organ complex). Based on this evidence, we propose to transfer these sinistral snails to the genus Hemiplecta. Molecular phylogenetic analyses further strongly support the monophyly of this sinistral lineage with respect to other members of Hemiplecta. This monophyletic clade consists of five members including three species that were previously classified as “Dyakia”, H. lahatensis, H. retrorsa and H. salangana, and two new species described herein, H. ligorica n. sp. and H. thailandica n. sp. This study also suggests that the anatomy of the gametolytic organ in the genus Hemiplecta corresponds well with the phylogenetic relationships and appears to be a taxonomically informative character, while the penial verge has little utility for generic recognition.


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