Vermehrung Und Schadwirkung Von Aphelenchoides Ritzemabosi Und Ditylenchus Dipsaci in Virusfreiem Und in Tmv-Infiziertem Tabak

Nematologica ◽  
1969 ◽  
Vol 15 (3) ◽  
pp. 334-336 ◽  
Author(s):  
B. Weischer
Keyword(s):  
Ditylenchus Dipsaci ◽  
Aphelenchoides Ritzemabosi

scholarly journals Conversion of sequence-characterized amplified region (SCAR) bands into high-throughput DNA markers based on RAPD technique for detection of the stem nematode Ditylenchus dipsaci in crucial plant hosts

2008 ◽  
Vol 53 (No. 3) ◽  
pp. 97-104 ◽  
Author(s):  
M. Zouhar ◽  
M. Marek ◽  
O. Douda ◽  
J. Mazáková ◽  
P. Ryšánek
Keyword(s):  
Cost Effective ◽  
Healthy Plant ◽  
Host Preferences ◽  
Sequence Characterized Amplified Region ◽  
Detection And Identification ◽  
Plant Hosts ◽  
Cost Effective Technique ◽  
Ditylenchus Dipsaci ◽  
Species Specific ◽  
Template Dna

<i>Ditylenchus dipsaci</i>, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of <i>D. dipsaci</i> control is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid, reliable, as well as cost effective technique is needed for identification of <i>D. dipsaci</i> in biological samples. This study describes the development of species-specific pairs of PCR oligonucleotides for detection and identification of the <i>D. dipsaci</i> stem nematode in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all <i>D. dipsaci</i> isolates. Two developed SCAR primer pairs were further tested using template DNA extracted from a collection of twelve healthy plant hosts; no amplification was however observed. The developed PCR protocol has proved to be quite sensitive and able to specifically detect <i>D. dipsaci</i> in artificially infested plant tissues.

Observations on resistance in white clover (Trifolium repens L.) to the stem nematode (Ditylenchus dipsaci (Kühn) Filipjev)

1988 ◽  
Vol 110 (1) ◽  
pp. 145-154 ◽  
Author(s):  
R. Cook ◽  
D. R. Evans
Keyword(s):  
White Clover ◽  
Trifolium Repens ◽  
Significant Positive Correlation ◽  
Leaf Size ◽  
Nematode Reproduction ◽  
Long Period ◽  
Plant Collections ◽  
Ditylenchus Dipsaci ◽  
Trifolium Repens L ◽  
Selection For

SummaryThe expression of symptoms of stem nematode reproduction on a total of 53 white clovers (26 cultivars, 14 genepools and 13 introductions from plant collections) was studied in a series of field and glasshouse experiments. Seedlings or stolon-tip cuttings were inoculated with nematodes and the clovers classified by the proportion of plants which developed symptoms. Significant differences were found between varieties although in each test the majority was intermediate between more resistant and susceptible extremes. There was significant positive correlation between tests, in spite of different inoculation methods and different average levels of susceptibility. Very large-leaved cv. Aran was more resistant than most other clovers tested, and small-leaved cv. S. 184 was more susceptible. There was no general correlation of leaf size with reaction to stemnematode. Small-leaved cv. Pronitro was also resistant while several large-leaved cultivars were susceptible. In observations of plants exposed to nematodes over a long period, either by sequential inoculations or through perpetuating latent infections, apparently resistant plants eventually succumbed and supported nematode reproduction. It has not been determined whether this was because selection for virulence in the nematodes had occurred.

Survival of Aphelenchoides Ritzemabosi (Schwartz) in Soil and Dry Leaves

Nematologica ◽  
1962 ◽  
Vol 7 (4) ◽  
pp. 309-316 ◽  
Author(s):  
N. French ◽  
Ruth M. Barraclough
Keyword(s):  
Aphelenchoides Ritzemabosi

Ditylenchus dipsaci. [Distribution map].

1999 ◽  
Author(s):  
Keyword(s):  
New York ◽  
Prince Edward Island ◽  
Russian Far East ◽  
Ornamental Plants ◽  
Far East ◽  
South Australia ◽  
Distribution Map ◽  
Mainland Portugal ◽  
Central Russia ◽  
Ditylenchus Dipsaci

Abstract A new distribution map is provided for Ditylenchus dipsaci (Kühn) Filipjev Nematoda: Tylenchida: Anguinidae Hosts: Many crop and ornamental plants. Information is given on the geographical distribution in EUROPE, Albania, Austria, Belarus, Belgium, Bosnia-Herzegovina, Bulgaria, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Mainland France, Germany, Greece, Mainland Greece, Hungary, Iceland, Ireland, Italy, Mainland Italy, Sicily, Latvia, Lithuania, Macedonia, Malta, Moldova, Netherlands, Norway, Poland, Portugal, Azores, Mainland Portugal, Romania, Central Russia Russian Far East, Southern Russia, Western Siberia, Slovakia, Slovenia, Spain, Mainland Spain, Sweden, Switzerland, UK, England and Wales, Scotland, Ukraine, Yugoslavia (Fed. Rep.), ASIA, Armenia, Azerbaijan, China, Gansu, Hebei, Henan, Shandong, Sichuan, Xinjiang, Cyprus, Republic of Georgia, Iran, Iraq, Israel, Japan, Honshu, Jordan, Kazakhstan, Korea Republic, Kyrgyzstan, Oman, Pakistan, Syria, Taiwan, Turkey, Uzbekistan, Yemen, AFRICA, Algeria, Kenya, Morocco, Reunion, South Africa, Tunisia, NORTH AMERICA, Canada, Alberta, British Columbia, Ontario, Prince Edward Island, Saskatchewan, Mexico, USA, Alabama, Arizona, California, Colorado, Hawaii, Idaho, Michigan, Montana, New York, North Carolina, Oregon, South Dakota, Utah, Virginia, Washington, Wyoming, CENTRAL AMERICA &#38; CARIBBEAN, Costa Rica, Dominican Republic, Haiti, SOUTH AMERICA, Argentina, Bolivia, Brazil, Minas Gerais, Paraiba, Parana, Rio Grande do Sul, Santa Catarina, Sao Paulo, Chile, Colombia, Ecuador, Paraguay, Peru, Uruguay, Venezuela, OCEANIA, Australia, New South Wales, South Australia, Tasmania, Victoria, Western Australia, New Zealand.

scholarly journals First Report of Ditylenchus dipsaci on Garlic in Minnesota

Plant Disease ◽  
2012 ◽  
Vol 96 (11) ◽  
pp. 1707-1707 ◽  
Author(s):  
D. S. Mollov ◽  
S. A. Subbotin ◽  
Carl Rosen
Keyword(s):  
The Body ◽  
Parasitic Nematodes ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Surrounding Water ◽  
Pharyngeal Bulb ◽  
First Report ◽  
Plant Parts ◽  
Ditylenchus Dipsaci

In the summer of 2011, two independent garlic samples from Morrison and Dakota counties and in 2012 one garlic sample from Carver county in Minnesota were submitted by commercial growers to the University of Minnesota Plant Disease Clinic for disease analyses. Symptoms of the above-ground plant parts were stunting and chlorosis. Symptoms of bulbs were necrosis, underdevelopment, and distortion. Upon microscopic examination, phytonematodes exuded into the surrounding water droplet. Nematodes were present in the protective leaves, abscission zone, and cloves in all submitted bulbs (n = 18) for analyses. Morphometric examination of females, males, and juveniles determined that they were Ditylenchus dipsaci. Nematodes extracted from garlic cloves were fixed in TAF (97 ml formalin [40%], 2 ml triethanolamine, and 91 ml dH2O). Morphological observations and measurements were made under an Olympus BX51 microscope equipped with a Nomarski differential interference contrast. Female (n = 6) measurements were: L = 1.411 to 1.636 mm, a = 38 to 44, b = 5.8 to 8.0, c = 14 to 17, stylet = 11.5 to 12.3 μm, V = 79 to 81%, and tail = 95 to 105 μm. The body was almost straight, when heat relaxed, lip region flattened, median bulb oval, and isthmus elongate and slender. The basal pharyngeal bulb overlapped the intestine. The post-vulval uterine branch was about half of vulva-anus distance. The tail was conoid with a pointed terminus. Male (n = 9) measurements were: L = 1.372 to 1.558 mm, a = 40 to 50, b = 6.5 to 7.0, c = 14 to 16, stylet = 11.5 to 12.3 μm, spicules = 22 to 27 μm, and gubernaculum = 9 to 10 μm. The bursa was leptoderan and spicules were curved with simple gubernaculum. Morphology and morphometrics of females and males of D. dipsaci from Minnesota generally fit the descriptions provided for the type and other populations by Hopper (1) and other authors. Several specimens were also taken for molecular identification. DNA extraction, PCR, and sequencing protocols were as described by Subbotin et al. (2). The TW81 and AB28 primers were used for amplification of ITS-rRNA region and the D2A and D3B primers were used for amplification of the D2-D3 expansion segments of 28S rRNA gene. Comparison of the ITS and D2-D3 of 28 rRNA gene sequences showed 100 and 99% identity with corresponding gene sequences of D. dipsaci published in the GenBank (2). The sequences were submitted in the GenBank under accession numbers JX123258 and X123259. This nematode problem has not been known to occur in either of these locations previously. The most likely source of introduction of D. dipsaci are imported garlic seed bulbs. To our knowledge, this is the first report of D. dipsaci affecting garlic or any other crops in Minnesota. The garlic produced in these locations was considered unmarketable and complete loss to the farmers. The presence of D. dipsaci could have a significant economic impact in the emerging multi-million dollar garlic industry in Minnesota. References: (1) D. J. Hooper. Ditylenchus dipsaci. CIH Descriptions of Plant-Parasitic Nematodes Set 1, No. 14, 1972. (2) S. A. Subbotin et al. Phytopathology 95:1308, 2005.

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