A comparative SEM study of the eggs of the Sepsidae (Diptera) with a cladistic analysis based on egg, larval and adult characters

1995 ◽  
Vol 26 (4) ◽  
pp. 425-438 ◽  
Author(s):  
Rudolf Meier

AbstractThe eggs of 21 species in 12 genera of Sepsidae were studied using scanning electron microscopy. All but the eggs of Ortalischema have respiratory filaments which aid in gas exchange. Judged by positional differences and the lack of a central canal, the two filaments of Orygma are not homologous to the single filament of most remaining sepsids. Hinton's hypothesis that egg filaments are plesiomorphic for the Sepsidae is therefore rejected. Three egg characters are added to an existing data set comprising 58 taxa and 85 larval and adult characters. The egg characters allow the unambiguous placement of Lasionemopoda, for which the larval and adult data set had suggested two different positions on equally parsimonious trees. Based on the position of Lasionemopoda, the evolution of the fore femora of the Sepsidae is briefly discussed. The egg morphology of the Sepsidae is also compared to the morphology of drosophiline eggs which also have egg filaments.

Zootaxa ◽  
2007 ◽  
Vol 1465 (1) ◽  
pp. 15-29 ◽  
Author(s):  
NICOLÁS UBERO-PASCAL ◽  
M. ANGELS PUIG

The chorionic patterns of Ephemeroptera eggs are very diverse and these have often been used for taxonomic and systematic purposes. In a great number of species, including Potamanthus luteus, Ephemera danica and Oligoneuriella rhenana, these egg features have been studied using light microscopy. However, current trends in egg morphology studies use scanning electron microscopy (SEM), so that the eggs of these species need to be re-described in order to establish morphological comparisons. The general chorionic features which have already been described in these three species are confirmed in our SEM study, although a more detailed description of both the architecture and arrangement of these can now be offered. In addition, this study has allowed us to note new morphological data, such as the chorionic reticulation in P. luteus and the complex extrachorion-adhesive layer in E. danica; classification of the lateral attachment structure in P. luteus and O. rhenana has been changed; and the variability of the polar cap observed in P. luteus underlines the care that must to be taken when selecting chorionic structures for taxonomic purposes.


2016 ◽  
Vol 8 (1) ◽  
pp. 469-472
Author(s):  
Padam Singh ◽  
Jagirti Madan ◽  
Nidhi Gupta

Scanning electron microscopy (SEM) study showed that miniature rice grain like egg shell of Hohorstiella rampurensis was covered by obliquely placed opercular disc. The apophyses were present only on one side of the egg shell. There were 25-35 long elongated rod like apophyses (apically turned), which were arranged in 3-4 rows and occurred in pair. The nature of egg shell of H. rampurensis characteristically differed from that of H. lata (the only species of Hohorstiella studied, so far). The study further indicates about the role of egg morphology as a guide to louse taxonomy.


2019 ◽  
Vol 57 (3) ◽  
pp. 745-754
Author(s):  
Francisco Augusto da Silva Ferreira ◽  
Rejane de Castro Simões ◽  
Ruth Leila Ferreira-Keppler ◽  
Jeronimo Alencar ◽  
Vera Margarete Scarpassa ◽  
...  

Abstract The eggs of three Amazonian species of the genus Mansonia (Diptera: Culicidae) were analyzed using morphological and morphometric characters. Eggs of Mansonia humeralis Dyar & Knab, 1916 were morphologically different from those of Mansonia titillans (Walker, 1848) and Mansonia amazonensis (Theobald, 1901), which were more similar to each other according to linear and geometric morphometry. A principal component analysis generated from elliptic Fourier contour data (PC1—92.6% and PC2—2.61%) indicated that Ma. amazonensis and Ma. titillans are more similar to each other than either is to Ma. humeralis. Discriminant multivariate analysis was highly accurate with only four classification errors and a 90% accuracy rate. The results indicate that the three Amazonian species can be precisely distinguished in the egg stage and that geometric morphometry based on elliptic Fourier contours is a promising method for distinguishing eggs of species of Mansonia. An identification key based on egg morphology is provided to distinguish the four Neotropical species.


1986 ◽  
Vol 64 (9) ◽  
pp. 1994-1997 ◽  
Author(s):  
M. El Gohary ◽  
M. Y. Kamel ◽  
M. H. Madbouly

External egg morphology of the camel tick Hyalomma dromedarii was studied. Injection of egg-laying females with 1% trypan blue resulted in incorporation of vital stain in the oviposited eggs. Daily measurements of fresh eggs revealed changes in egg length and width during embryonic development. Scanning electron microscopy showed the outermost layer of the egg to be spongy and porous containing clear pits of different sizes. An elevated longitudinal hatching ridge encircled the egg chorion. The development of cephalothorax, appendages, Malpighian tubules, and rectal sacs were monitered.


2019 ◽  
Vol 11 (13) ◽  
pp. 14722-14744
Author(s):  
Gour Gopal Satpati ◽  
Ruma Pal

Scanning electron microscopy (SEM) is the most modern technique for plankton research.  The present paper deals with the taxonomy and morphology of some rare and endangered planktonic chlorophytes in relation to scanning electron microscopy.  Water samples from the distinct water body of the Sundarbans have been concentrated and examined by scanning electron microscopy.  A total of 45 species, of which 17 species of Scenedesmaceae, 10 species each of Hydrodictyaceae and Desmidiaceae, five species of Chlorococcaceae, two species of Selenastraceae and only one species of Chlorellaceae were recorded from the study site.  Some species were recorded as new and rare from the study area.  About 18 species including nine extremely rare, seven occasional, six frequent, four sporadic and one abundant was recorded in the present study.  A detailed taxonomic description with line drawings is also included in the present communication.


2016 ◽  
Vol 48 (3) ◽  
pp. 388 ◽  
Author(s):  
S. Karschová ◽  
J. Hajer

The spinning apparatus and silk of <em>Segestria senoculata</em> were studied with the use of scanning electron microscopy and light microscopy, which confirmed the presence of four categories of spigots connected with four types of spinning glands (<em>i.e</em>., <em>Glandulae ampullaceae major, Glandulae ampullaceae minor, Glandulae piriformes and Glandulae pseudaciniformes</em>). New data about the morphology of spinnerets and spigots were obtained for both the adults and nymphal stages of both sexes. For the first time the silken threads of retreats, signal threads and attachment discs of the members of <em>Segestria</em> were subjected to a detailed SEM study. The data resulting from studying the spinning apparatus of <em>S. senoculata</em> was compared to current knowledge of the silk producing systems of families belonging to the Dysderoidea superfamily. Silks that are emitted from spigots in the course of retreat construction are not (similarly to the other dysderoids) processed by the spider’s legs during the subsequent process of hardening. Apart from the major ampullate glands/spigots, segestriids also possess developed minor ampullate glands. Minor ampullate threads are used by <em>S. senoculata</em> spiders when making their signal threads.


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