Induction of Tyloses in Eucalyptus Globulus 'chips'

IAWA Journal ◽  
1999 ◽  
Vol 20 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Mathew A. Leitch ◽  
Rodney A. Savidge ◽  
Geoff M. Downes ◽  
Irene L. Hudson
Keyword(s):  
Acetic Acid ◽  
Eucalyptus Globulus ◽  
Culture Media ◽  
Inhibitory Effect ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Control Medium ◽  
Water Control ◽  
Significant Inhibitory Effect ◽  
Defined Culture

Cambial stem chips containing intact cambium between xylem and phloem, or with the phloem layer removed, were cut from the main stem axis of four-year-old Eucalyptus globulus during the winter and grown under controlled environmental conditions for seven weeks on fully defined culture media . Light microscopy revealed that tyloses were induced in sapwood vessels in the region adjacent to the cambium within these stem chips. When incubated in autoclaved double-distilled water (control medium) tyloses were produced in 3.7% and 4.7% of vessels in chips with the phloem layer intact and removed, respectively. When non-hormonal ingredients were included, tyloses developed in 69.5% and 76.1% of vessels in chips with the phloem layer intact and removed, respectively. Addition of 1.0 mg l-1 of l-naphthalene acetic acid (NAA, a synthetic auxin) to the medium had a slight, but significant, inhibitory effect on tylosis formation.

scholarly journals Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal

2016 ◽  
Vol 4 (2) ◽  
pp. 155-160
Author(s):  
Lila Bahadur Magar ◽  
Nisha Shrestha ◽  
Saraswoti Khadka ◽  
Jay Raj Joshi ◽  
Jibaraj Acharya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Royalty Rate ◽  
Paulownia Tomentosa ◽  
Shoot Bud ◽  
Plastic Bags

Paulownia tomentosa Steud is a fast growing multipurpose tree. In vitro propagation using nodal explants of Paulownia tomentosa was performed by manipulating amount of cytokinin and auxin in culture media. Shoot bud proliferation from explants were assessed in Murashige and Skoog (MS) medium supplemented with various amounts of hormones such as a) 0.5-2.5 mg/l benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA), b) 0.5-2.5 mg/l BAP and 0.5-2.5 mg/l kinetin (KN) and c) 0.5- 2.5 mg/l BAP and 0.1 mg/l indole-3-acetic acid (IAA). In the present study, we found that hormone combination of BAP and NAA gave optimum growth results. MS medium enriched with 2.0 mg/l BAP and 0.1 mg/l IAA resulted a similar outcome but took 3-4 weeks with respect to the same medium enriched with 1.0 mg/ml BAP and 0.1 mg/ml NAA, which showed response within 2-3 weeks. Shoot length of 2.5-3.5 cm with 3-4 nodes and 8-12 leaves were obtained on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. The acclimatization of explants was done in a polyhouse at 20±5oC for 2-6 weeks. Rooting has been induced in nonsterile sand. Rooted plants were transferred to plastic bags containing mixture of soil, sand and compost in the ratio of 1:1:1.Besides aforementioned issues, there are several other challenges associated with in vitro propagation of P. tomentosa. The plants were established (90%) on MS medium enriched with BAP and NAA and adapted ex vitro with surviving up to 80%. People received an opportunity with this plant because it grows fast and can generate income in 10 years in comparison with others, but at the same time people also have been facing the challenges for plantation of P. tomentosa as government of Nepal does not formulate necessary national policies, legislations and regulatory frameworks in its favor. Thus, system should be developed to set royalty rate of P. tomentosa recognizing its lifetime value.Int J Appl Sci Biotechnol, Vol 4(2): 155-160

scholarly journals Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus

2010 ◽  
Vol 34 (5) ◽  
pp. 1245-1252 ◽  
Author(s):  
Marcos Nopper Alves
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Shoot Regeneration ◽  
Culture Media ◽  
Adventitious Shoot ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Adventitious Shoot Regeneration ◽  
Popular Medicine ◽  
Superior Genotype

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid (NAA) (1.0 or 2.0 mg L-1), after 40 days of culture. Best callus production was obtained after 30 days of petioles' culture on gelled MS medium with 2,4 dichlorophenoxyacetic acid (2,4-D) (5.0 mg L-1) combined with BAP (1.0 mg L-1). Successful shoot regeneration from callus was achieved when MS medium supplemented with zeatin (ZEA) (0.1 mg L-1) alone or combined with 2,4-D (1.0 or 5.0 mg L-1) was inoculated with friable callus obtained from petioles. All shoots were rooted by inoculation on MS medium supplemented with indole-3-acetic acid (IAA) (1.0 mg L-1). Rooted plants transferred to potting soil were successfully established. All in vitro regenerated plantlets showed to be normal, without morphological variations, being also identical to the source plant. Our study has shown that C. glaziovii can be propagated by tissue culture methods, allowing large scale multiplication of superior plants for pharmacological purposes.

scholarly journals Physiological potential of piquin pepper seeds in response to pregermination treatments

2021 ◽  
Vol 45 ◽  
Author(s):  
Miguel Ángel Cano-González ◽  
Benjamín Abraham Ayil-Gutiérrez ◽  
Rafael Delgado-Martínez ◽  
Eduardo Osorio-Hernández ◽  
José Antonio Rangel-Lucio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Wild Plant ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

scholarly journals DEFOLIATION OF WOODY CUT STEMS WITH PREHARVEST, LESS TOXIC CHEMICAL AND POSTHARVEST ENVIRONMENTAL METHODS

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 881e-881
Author(s):  
Lane Greer ◽  
John M. Dole
Keyword(s):  
Acetic Acid ◽  
Woody Plants ◽  
Tap Water ◽  
Pelargonic Acid ◽  
Distilled Water ◽  
Toxic Chemical ◽  
Water Control ◽  
Salix Matsudana ◽  
Irrigation Treatments

Six defoliants were applied in fall and tested for their efficacy in preharvest defoliation of field grown curly willow (Salix matsudana `Tortuosa'), american bittersweet (Celastrus scandens), and american beautyberry (Callicarpa americana). Defoliants included acetic acid, chelated copper, crop oil concentrate (COC), ethephon, dimethipin plus COC, pelargonic acid, and a tap water control. For chelated copper, a concentration of 800 mg·L–1 was most effective at promoting defoliation, providing 100% defoliation of american bittersweet and 76% defoliation of american beautyberry. For curly willow and american beautyberry, all concentrations of dimethipin produced good or excellent defoliation. Increasing concentrations of ethephon from 200 to 2500 mg·L–1 increased defoliation from 0% to 67%. Pelargonic acid was not effective at promoting defoliation of woody plants at the concentrations used. In an experiment conducted during spring using containerized curly willow, irrigation was stopped for 0, 3, or 6 days before defoliants were applied, but none of the irrigation treatments promoted defoliation. In a postharvest study using cut curly willow, stems were held in distilled water at 5, 20, or 35 °C for 1, 3, 5, or 7 days. Holding cut stems of curly willow at 20 °C promoted 68% defoliation, compared to 53% or 28% for 5 or 35 °C, respectively.

scholarly journals Inhibitory Effect of Nucleotides on the Multiplication of Babesia gibsoni in Canine Erythrocytes

1970 ◽  
Vol 2 (1) ◽  
pp. 59-61
Author(s):  
MA Hossain ◽  
O Yamato ◽  
M Yamasaki ◽  
Y Maede
Keyword(s):  
In Vitro Culture ◽  
Key Words ◽  
Culture Media ◽  
Inhibitory Effect ◽  
Final Concentration ◽  
Inhibitory Effects ◽  
Babesia Gibsoni ◽  
Significant Inhibitory Effect

The inhibitory effects of nucleotides on the multiplication of Babesia gibsoni was studied on the in vitro culture of canine erythrocytes. B. gibsoni was cultivated at 37°C for 3 days under a humidified atmosphere containing 5% C02, 5% O2, and 90% N2 in  culture media with no nucleotides (Control), cytidine 5'-monophosphate (5'-CMP), uridine 5'-monophosphate (5'-UMP), uridine 3'-monophosphate (3'-UMP), thymidine 3'-monophosphate (3'-TMP) and inosine 5'-monophosphate (5'-IMP), adenine 5'-monophosphate (5'-AMP) and guanine 5'-monophosphate (5'-GMP), in which a final concentration of nucleotides was 5 mM. The adding of 5'-CMP, 5'-UMP, 5'-IMP, 5'-AMP and 5'-GMP as artificial nucleotides significantly (p < 0.01) inhibited the multiplication of B. gibsoni in canine erythrocytes. Further more, the 5'-CMP and 5'-IMP showed the dose-dependently significant inhibitory effect on the multiplication B. gibsoni. It may be concluded from these results that the accumulation of specific nucleotides might have inhibited the multiplication of B. gibsoni in canine erythrocytes. Key words: Babesia gibsoni; multiplication inhibition; canine erythrocytes; nucleotides doi: 10.3329/bjvm.v2i1.1937 Bangl. J. Vet. Med. (2004). 2 (1) : 59-61

scholarly journals Auxin type and dilution vehicles on vegetative propagation of Varronia curassavica Jacq. and Melaleuca alternifolia Cheel

2021 ◽  
Vol 27 (2) ◽  
pp. 238-246
Author(s):  
Marcelo Pereira ◽  
Lilian Fernanda Sfendrych Gonçalves ◽  
Erik Nunes Gomes ◽  
Überson Boaretto Rossa ◽  
Cícero Deschamps
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Stem Cuttings ◽  
Melaleuca Alternifolia ◽  
Indolebutyric Acid ◽  
Naoh Solution

Abstract Studies on the propagation of medicinal and ornamental plant species show of great relevance as they contribute to the domestication, cultivation and production of these species. However, there are not enough information concerning the interaction of the different plant growth regulators and the recommended dilution vehicles. This work aimed to evaluate the effects of sodium hydroxide (NaOH) and ethanol as rooting inducers, as well as their performance as vehicles for diluting indolebutyric acid (IBA) and naphthalene acetic acid (NAA) in stem cuttings of the species Varronia curassavica and Melaleuca alternifolia. Stem cuttings of both species were submitted to the treatments: control with distilled water, hydroethanolic solution (50% v v-1), NaOH solution in distilled water, indolebutyric acid diluted in hydroethanolic solution, indolebutyric acid diluted in NaOH solution, naphthalene acetic acid diluted in hydroethanolic acid and naphthalene acetic acid diluted in NaOH solution (0.5 N). The design used was completely randomized, with the averages compared by the Scott-Knott test at 5% probability. Better rooting of the cuttings was observed when IBA was applied using both dilution vehicles. For M. alternifolia, percentages of average survival of 26.43% were verified. Rooting was more satisfactory when using NaOH as a dilution vehicle for both IBA (16.66%) and NAA (23.33%). For V. curassavica, IBA (2000 mg L-1) is the most suitable plant regulator, diluted in both hydroethanolic solution and in NaOH, while for M. alternifolia, NaOH is recommended as a dilution vehicle for plant growth regulators NAA (500 mg L-1) and IBA (500 mg L-1). When applied isolated, the dilution vehicles do not stimulate and do not harm the rooting of the two species.

scholarly journals Defoliation of Woody Cut Stems with Preharvest, Less Toxic Chemical and Postharvest Environmental Methods

2005 ◽  
Vol 15 (2) ◽  
pp. 376-380
Author(s):  
Lane Greer ◽  
John M. Dole
Keyword(s):  
Acetic Acid ◽  
Woody Plants ◽  
Tap Water ◽  
Pelargonic Acid ◽  
Distilled Water ◽  
Toxic Chemical ◽  
Water Control ◽  
Salix Matsudana ◽  
Irrigation Treatments

Six defoliants were applied in fall and tested for their efficacy in preharvest defoliation of fieldgrown curly willow (Salix matsudana `Tortuosa'), american bittersweet (Celastrus scandens), and american beautyberry (Callicarpa americana). Defoliants included acetic acid, chelated copper, crop oil concentrate surfactant (COC), ethephon, dimethipin plus COC, pelargonic acid, and a tap water control. For chelated copper, a concentration of 800 mg·L–1 (ppm) was most effective at promoting defoliation, providing 100% defoliation of american bittersweet and 76% defoliation of american beautyberry. For curly willow and american beautyberry, all concentrations of dimethipin produced good or excellent defoliation. Increasing concentrations of ethephon from 200 to 2500 mg·L–1 increased defoliation from 0% to 67%. Pelargonic acid was not effective at promoting defoliation of woody plants at the concentrations used. In an experiment conducted during spring using containerized curly willow, irrigation was stopped for 0, 3, or 6 days before defoliants were applied, but none of the irrigation treatments promoted defoliation. In a postharvest study using cut curly willow, stems were held in distilled water at 5, 20, or 35 °C (41.0, 68.0, or 95.0 °F) for 1, 3, 5, or 7 days. Holding cut stems of curly willow at 20 °C promoted 68% defoliation, compared to 53% or 28% for 5 or 35 °C, respectively.

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR &amp; GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Acetic Acid ◽  
Factorial Design ◽  
Vitamin B1 ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

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