Apoptosis Signal-Regulating Kinase 1–Mediated Signaling Pathway Regulates Nitric Oxide–Induced Activator Protein-1 Activation in Human Bronchial Epithelial Cells

2003 ◽  
Vol 167 (6) ◽  
pp. 856-861 ◽  
Author(s):  
Itsuro Jibiki ◽  
Shu Hashimoto ◽  
Shuichiro Maruoka ◽  
Yasuhiro Gon ◽  
Atsushi Matsuzawa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Signaling Pathway ◽  
Bronchial Epithelial Cells ◽  
Activator Protein 1 ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Activator Protein

An electrogenic amino acid transporter in the apical membrane of cultured human bronchial epithelial cells

1998 ◽  
Vol 275 (5) ◽  
pp. L917-L923 ◽  
Author(s):  
Luis J. V. Galietta ◽  
Luciana Musante ◽  
Leila Romio ◽  
Ubaldo Caruso ◽  
Annarita Fantasia ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Apical Membrane ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Bronchial Epithelial Cells ◽  
Maximal Effect ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

We performed Ussing chamber experiments on cultured human bronchial epithelial cells to look for the presence of electrogenic dibasic amino acid transport. Apical but not basolaterall-arginine (10–1,000 μM) increased the short-circuit current. Maximal effect and EC50were ∼3.5 μA/cm2and 80 μM, respectively, in cells from normal subjects and cystic fibrosis patients. The involvement of nitric oxide was ruled out because a nitric oxide synthase inhibitor ( NG-nitro-l-arginine methyl ester) did not decrease the arginine-dependent current. Apicall-lysine,l-alanine, andl-proline, but not aspartic acid, were also effective in increasing the short-circuit current, with EC50values ranging from 26 to 971 μM. Experiments performed with radiolabeled arginine demonstrated the presence of an Na+-dependent concentrative transporter on the apical membrane of bronchial cells. This transporter could be important in vivo to maintain a low amino acid concentration in the fluid covering the airway surface.

scholarly journals Estrogen induces nitric oxide production in human bronchial epithelial cells

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Elizabeth A Townsend ◽  
Michael A Thompson ◽  
Stephen D Cassivi ◽  
Christina M Pabelick ◽  
Y S Prakash
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Nitric Oxide Production ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Oxide Production
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