scholarly journals Morphology of Metzgeria conjugata Lindb. (Metzgeriales, Hepaticopsida)

1992 ◽  
Vol 6 (1) ◽  
pp. 65-69
Author(s):  
Denise Pinheiro Da Costa ◽  
Raul Dodsworth Machado
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Outer Surface ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Epidermal Cells ◽  
Scanning Electron

Scanning electron microscopy and light microscopy were used to elucidate the morphology of Metzgeria conjutata Lindb. and confirm the presence of 2 rows of epidermal cells on the dorsal surface, (21-3) rows on the ventral surface, midrib with cells in (3-51-6) tiers; hirsute, short hairs, straight on the thallus-margin and on the ventral surface of midrib; marginal hairs paired, single or in groups of three; male branches globose or subglobose; female involucres obovate and hirsute at the margin, calyptra fleshy, pyriform to club-shaped, hirsute on the outer surface, hairs long and straight.

Scanning electron microscopy of the integument of schistosoma rodhaini

1986 ◽  
Vol 44 ◽  
pp. 132-133
Author(s):  
P. Evers ◽  
C. Schutte ◽  
C. D. Dettman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Oral Sucker ◽  
Adult Male ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Sensory Receptors ◽  
East African ◽  
Dorsal Midline ◽  
Scanning Electron

S.rodhaini (Brumpt 1931) is a parasite of East African rodents which may possibly hybridize with the human schistosome S. mansoni. The adult male at maturity measures approximately 3mm long and possesses both oral and ventral suckers and a marked gynaecophoric canal. The oral sucker is surrounded by a ring of sensory receptors with a large number of inwardly-pointing spines set into deep sockets occupying the bulk of the ventral surface of the sucker. Numbers of scattered sensory receptors are found on both dorsal and ventral surfaces of the head (Fig. 1) together with two conspicuous rows of receptors situated symmetrically on each side of the midline. One row extends along the dorsal surface of the head midway between the dorsal midline and the lateral margin.

scholarly journals Histological Variation in Plethodontid Digital Surfaces [University of Findlay]

2019 ◽  
Author(s):  
Megan Pasternak ◽  
Justin Rheubert
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Morphology ◽  
Light Microscopy ◽  
Morphological Evolution ◽  
Ventral Surface ◽  
Cellular Level ◽  
Plethodontid Salamanders ◽  
And Function ◽  
Scanning Electron

Despite numerous investigations into the morphology and function of toe pads in many species, most notably anurans and geckonids, there is relatively little knowledge on salamander digit morphology. To date, toe morphology in salamanders has been limited to Desmognathus fuscus, Ambystoma maculatum, Bolitoglossa sp., and Aneides aeneus. The limited studies to date have shown variation inter- and intra-specifically but have not investigated numerous taxa within a given family which may provide deeper insights into the causes of variation (phylogenetic vs ecological pressures). Therefore, to test hypotheses concerning the presence of variation in the ventral digital surface of plethodontid salamanders, we plan to use various microscopy methodologies to view the ventral surface of the digital tips of three species from three different genera within the Plethodontidae: Desmognathus, Eurycea, and Plethodon. Toe pads will be characterized grossly using scanning electron microscopy, histologically using light microscopy, and ultrastructurally using transmission electron microscopy. Preliminary results suggest that all three species investigated display enlarged surfaces. Surface morphology (assessed via scanning electron microscopy) varies between species at a gross level concerning the shape and overall orientation of the enlarged surface. Surface morphologies include a well-developed circular pad (D. fuscus), a well-developed oval pad (P. cinereus), and a poorly developed circular pad (E. cirrigera). Furthermore, surface morphology appears to vary at the cellular level as well, with Desmognathus having polygonal squamous cells with microprojections and Eurycea having polygonal cells with nanopillars in a honeycomb arrangement. These differences may be attributed to differences in habitat preference as the three species tested include a terrestrial, semi-aquatic, and aquatic dwelling species. However, further investigation including light microscopy and enhanced scanning electron microscopy are needed. Further understanding of the morphological variation will aid in our understanding of ecomorphology and understanding of morphological evolution in amphibians.

scholarly journals Characteristics of the surface of the epidermis in floral nectaries and the receptacle of mountain ash (Sorbus aucuparia L.)

2012 ◽  
Vol 60 (2) ◽  
pp. 23-30
Author(s):  
Agata Konarska
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Outer Surface ◽  
Flower Development ◽  
Epidermal Cells ◽  
Sorbus Aucuparia ◽  
Mountain Ash ◽  
Floral Nectaries ◽  
Scanning Electron

The structure of receptacular surfaces of floral nectaries at two flowering stages and the structure of the outer surface of the receptacle of <i>Sorbus aucuparia</i> were investigated using scanning electron microscopy. Changes in the development of the cuticular epithelium of the nectary epidermis and differences in the degree of aperture of stomata were observed. Increased undulation of the gland surface was found during flower development. Numerous stomata were situated slightly below the level of epidermal cells of the nectary. At the pollination stage, open pores or pores surrounded by the cuticular epithelium were observed, as well as covered by dried secretion. Dried nectar in the form of patches was also visible on the surface of the gland. Stomata of the outer surface of the receptacle were located on protrusions and surrounded by the cuticular epithelium.

scholarly journals Scanning electron microscopy on Gotocotyla acanthura (Monogenea, Gotocotylidae) from Pomatomus saltatrix (Ostheichthyes, Pomatomidae) in Brazil

2011 ◽  
Vol 20 (4) ◽  
pp. 342-346 ◽  
Author(s):  
Maria Clara Pamplona-Basilio ◽  
Helene Santos Barbosa ◽  
Simone Chinicz Cohen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Genital Atrium ◽  
Posterior Extremity ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
The Body ◽  
Pomatomus Saltatrix ◽  
Muscular Structure ◽  
Scanning Electron

Gotocotyla acanthura (Parona & Perugia, 1896) Meserve, 1938 collected from the gills of Pomatomus saltatrix from the coast of the state of Rio de Janeiro state was analyzed using scanning electron microscopy (SEM). The study demonstrated the presence of a buccal cavity, a genital atrium on the ventral surface and a muscular structure on the dorsal surface at the level of the body constriction. An elongated haptor with 80 to 120 pedunculated clamps symmetrically distributed in two rows, with rib-like thickenings and a curved lappet bearing a pair of hooks at the posterior extremity of the body were also observed. The cirrus could be seen protruding from the genital atrium, armed with pectinate spines along its length and presenting up to eight pointed spines around the genital atrium.

scholarly journals Anatomical Observations of Anthocyanin Rich Cells in Apple Skins

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 733-736 ◽  
Author(s):  
Ro-Na Bae ◽  
Ki-Woo Kim ◽  
Tae-Choon Kim ◽  
Seung-Koo Lee
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Outer Layer ◽  
Epidermal Cells ◽  
Envelope Membrane ◽  
Skin Cells ◽  
Red Color ◽  
Fuji Apple ◽  
Scanning Electron

Anatomical observations of anthocyanin rich cells in `Fuji' apple skins were carried out by light microscopy and electron microscopy. Apple skins with fully developed red color had more layers of anthocyanin-containing epidermal cells than those of green skins. The density of anthocyanin was high in cells of the outer layer of the fruit skins and gradually decreased inward to the flesh. Anthocyanins were frequently found in clusters or in agglomerations that were round in the epidermal cells of the red skins. They accumulated in the inner side of developed vacuoles. Transmission and scanning electron microscopy revealed that the shapes of anthocyanins were cluster style, indeterminable forms, or complete spheres. Anthocyanin seemed to be synthesized around the tonoplast and condensed on the inward side of the vacuole. There was no distinct envelope membrane on the anthocyanin granule in the vacuoles of apple skin cells.

Micromorphological and anatomical studies on petals of 11 Turkish Onosma L. (Boraginaceae) taxa

2009 ◽  
Vol 16 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Öznur Ergen Akçin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dorsal Surface ◽  
Epidermal Cells ◽  
Species Level ◽  
Cell Type ◽  
Anatomical Structures ◽  
Anatomical Studies ◽  
Scanning Electron

The petal epidermis and petal anatomy of 11 taxa of Onosma L. were studied using light and scanning electron microscopy. Differences in epidermal patterns were observed on the dorsal surface. Domed epidermal cells and non-papillae epidermal cells were seen on the petal surface. Papillae types were lithops, cone-like, finger-like or skittle-cell type. Areolate and rugose types were observed on the non-papillae surface. Principal anatomical structures of petals were similar. Micromorphological features on dorsal surfaces of petals could be useful for species-level diagnosis along with other selected morphological and micromorphological features.

The surface topography of Benedenia sp. (Monogenea: Capsalidae)

1981 ◽  
Vol 59 (10) ◽  
pp. 1941-1946 ◽  
Author(s):  
D. K. Cone ◽  
M. Beverley-Burton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Topography ◽  
Coastal Waters ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
The Body ◽  
Middle Zone ◽  
Tegumental Surface ◽  
Scanning Electron

The tegumental surface of Benedenia sp. (Monogenea: Capsalidae), a skin-dwelling parasite of Haemulon sciurus (blue-striped grunt) from coastal waters of Bermuda, was examined using scanning electron microscopy. The dorsal surface of the body and haptor is covered with comparatively long, flexible microvilli (2 to 3 μm in length; density 5/μm2), and the ventral surface of the body is covered with short, stubby microvilli (0.5 μm in length; density 10/μm2). The surface of the two anterior adhesive discs has three transverse zones which differ morphologically: the anterior region has a reticulate texture with uniciliated structures arising from some of the elevated ridges; the middle zone has a dense mat of short microvilli with scattered uniciliated structures, each of which is surrounded by a collar of shorter protuberances; the posterior zone is smooth and lacks ciliated structures. The ventral surface of the haptor is also smooth with the bare extremities of the accessory sclerites and the anterior and posterior hamuli protruding. The surface topography of Benedenia sp. is compared with that of other monogeneans.

Stigma and Style Morphology in Relation to Taxonomy and Breeding Systems in Eucalyptus and Angophora (Myrtaceae)

1986 ◽  
Vol 34 (5) ◽  
pp. 569 ◽  
Author(s):  
DJ Boland ◽  
M Sedgley
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Outer Surface ◽  
Breeding Systems ◽  
Cohesive Group ◽  
Varying Length ◽  
Scanning Electron

The stigma and style of 94 species of Eucalyptus and two species of Angophora were studied by scanning electron microscopy and/or light microscopy. All species had papillate stigmas and a stylar canal of varying length. Angophora species had mop-like stigmas with long papillae that were very similar in appearance to those of the red bloodwood group of the Corymbia, e.g. E. gummifera. The spotted gum group of the Corymbia had mop-like stigmas with short papillae and the yellow bloodwoods had tapered stigmas. The latter group was also charaderised by an extremely thick cuticle on the outer surface of the style, over 100 �m in thickness in E. watsoniana. All species in Blakella had tapered stigmas with a lobed surface and relatively few short papillae. The stylar canal had no cuticle in E. papuana. Eudesmia is a variable subgenus with E. erythrocorys unusual in having long multicellular papillae. Most Symphyomyrtus species had blunt or pinhead-shaped lobed stigmas with a heavily cutinised stylar canal. E. deglupta and E. microcorys did not conform to this pattern and had mop-shaped stigmas with long papillae. Monocalyptus species had blunt stigmas with few papillae and hollow styles and appeared to form a cohesive group. On the basis of stigma and style morphology Angophora is more similar to Corymbia than to Blakella. E. deglupta and E. microcorys are distinct from other Symphyomyrtus species studied. E. trachyphloia and E. jacobsiana are more similar to E. gummifera than to E. watsoniana or other yellow bloodwoods.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

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