Pseudopyricularia hedjaroudii sp. nov. (Pyriculariaceae, Magnaporthales): a novel species from Iran

Phytotaxa ◽  
2021 ◽  
Vol 479 (1) ◽  
pp. 95-104
Author(s):  
GOLZAR GHORBANI ◽  
ADEL PORDEL ◽  
MOHAMMAD JAVAN-NIKKHAH
Keyword(s):  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Large Subunit ◽  
The North ◽  
Phylogeny Analysis ◽  
Morphological Criteria ◽  
North Of Iran

In a survey of pyricularia-like fungi on different plants, especially on sedges in the north of Iran, we identified two typical isolates characteristics of the genus Pseudopyricularia. Phylogeny analysis based on DNA sequences of internal transcribed spacer regions (ITS-rDNA), the largest subunit of the RNA polymerase II (RPB1), calmodulin (CAL), and large subunit of the ribosomal RNA (LSU) gene regions, and morphological criteria confirmed the new species of Pseudopyricularia. Pseudopyricularia hedjaroudii sp. nov., isolated from the leaves of Cyperus sp., is described and illustrated. Conidia of the Ps. hedjaroudii are obclavate, obpyriform, and 1-septate distinct from other allied species in size and shape.

A polyphasic approach to characterise two novel species of Phoma (Didymellaceae) from China

Phytotaxa ◽  
2015 ◽  
Vol 197 (4) ◽  
pp. 267-281 ◽  
Author(s):  
Qian Chen ◽  
KE ZHANG ◽  
GUOZHEN ZHANG ◽  
LEI CAI
Keyword(s):  
Rna Polymerase Ii ◽  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Polyphasic Approach ◽  
Nrdna Its ◽  
Two New Species ◽  
Viburnum Odoratissimum ◽  
Β Tubulin

Phoma odoratissimi sp. nov. on Viburnum odoratissimum and Syringa oblate, and Phoma segeticola sp. nov. on Cirsium segetum from China are introduced and described, employing a polyphasic approach characterising morphological characteristics, host association and phylogeny. Both species are the first records of Phoma species on their respective hosts. Multi-locus phylogenetic tree was inferred using combined sequences of the internal transcribed spacer regions 1 & 2 and 5.8S nrDNA (ITS), and partial large subunit 28S nrDNA region (LSU), β-tubulin (TUB) region and RNA polymerase II (RPB2) region. The two new species clustered in two separate and distinct lineages, and are distinct from their allied species.

Reticulascaceae hyphomycetes from submerged wood in Yunnan, China

Phytotaxa ◽  
2018 ◽  
Vol 348 (3) ◽  
pp. 187 ◽  
Author(s):  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
ZONG-LONG LUO ◽  
HONG-YAN SU ◽  
ABDULLAH M. AL-SADI ◽  
RATCHADAWAN CHEEWANGKOON
Keyword(s):  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Yunnan Province ◽  
Large Subunit ◽  
Protein Subunit ◽  
Morphological Examination ◽  
Gene Encoding ◽  
Its Regions ◽  
Taxonomic Novelties

Asexual members of Reticulascaceae (Glomerellales, Hypocreomycetidae) are generally hyphomycetes and usually found as saprobes occurring on various hosts in lentic habitats. A brief survey of the freshwater fungi in Yunnan Province, China, was carried out and four species of Reticulascaceae were isolated. Morphological examination and sequence analysis of large subunit of the nuclear ribosomal RNA (LSU), RNA polymerase II gene encoding the second largest protein subunit (RPB2) and internal transcribed spacer (ITS) regions provide evidence for two taxonomic novelties of Kylindria (K. aquatica and K. chinensis). Two other collections comprised two previously described species of Cylindrotrichum (C. clavatum and C. gorii). Descriptions and illustrations of the above taxa are provided and new species are discussed with comparable taxa.

scholarly journals Genetic diversity and biogeography in Chaetomorpha melagonium (Ulvophyceae, Cladophorales) based on internal transcribed spacer (ITS rDNA) sequences

2020 ◽  
Author(s):  
C Boedeker ◽  
F Leliaert ◽  
Giuseppe Zuccarello
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
Base Pair ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Point Mutations ◽  
Its Sequences ◽  
The Arctic ◽  
Lsu Rdna ◽  
The North

© 2017 Walter de Gruyter GmbH, Berlin/Boston. Chaetomorpha melagonium is a morphologically distinct species of green algae that occurs throughout the North Atlantic, the North Pacific and the Arctic Ocean. In this study, we analyzed the intraspecific genetic diversity among 14 samples of C. melagonium from across the distribution range based on nuclear large subunit ribosomal DNA (LSU rDNA) and rDNA internal transcribed spacer (ITS) DNA sequences. All samples had identical LSU sequences. The ITS sequences had very few mutations that nevertheless divided the specimens into two groups: one included samples from Iceland, Svalbard, Massachusetts and Alaska with identical ITS sequences; members of this group differed in samples from Europe (France, Germany, Scotland, Sweden, and Wales) by three mutations (two point mutations and one five base pair indel). The European specimens had identical ITS sequences with the exception of a single sample from Brittany that differed by one base pair. The maximum ITS sequence divergence within the samples of C. melagonium was less than 0.5%. This low intraspecific variation in the frequently used highly variable ITS region is discussed in the context of past geological and climatic scenarios.

scholarly journals Genetic diversity and biogeography in Chaetomorpha melagonium (Ulvophyceae, Cladophorales) based on internal transcribed spacer (ITS rDNA) sequences

2020 ◽  
Author(s):  
C Boedeker ◽  
F Leliaert ◽  
Giuseppe Zuccarello
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
Base Pair ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Point Mutations ◽  
Its Sequences ◽  
The Arctic ◽  
Lsu Rdna ◽  
The North

© 2017 Walter de Gruyter GmbH, Berlin/Boston. Chaetomorpha melagonium is a morphologically distinct species of green algae that occurs throughout the North Atlantic, the North Pacific and the Arctic Ocean. In this study, we analyzed the intraspecific genetic diversity among 14 samples of C. melagonium from across the distribution range based on nuclear large subunit ribosomal DNA (LSU rDNA) and rDNA internal transcribed spacer (ITS) DNA sequences. All samples had identical LSU sequences. The ITS sequences had very few mutations that nevertheless divided the specimens into two groups: one included samples from Iceland, Svalbard, Massachusetts and Alaska with identical ITS sequences; members of this group differed in samples from Europe (France, Germany, Scotland, Sweden, and Wales) by three mutations (two point mutations and one five base pair indel). The European specimens had identical ITS sequences with the exception of a single sample from Brittany that differed by one base pair. The maximum ITS sequence divergence within the samples of C. melagonium was less than 0.5%. This low intraspecific variation in the frequently used highly variable ITS region is discussed in the context of past geological and climatic scenarios.

Phaeoclavulina pseudozippelii sp. nov. (Gomphales, Basidiomycota) from Northern Thailand

Phytotaxa ◽  
2018 ◽  
Vol 362 (2) ◽  
pp. 211
Author(s):  
NOPPARAT WANNATHES ◽  
RATTIKAN KAEWKETSRI ◽  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
SAISAMORN LUMYONG
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
National Park ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Taxonomic Position ◽  
Northern Thailand ◽  
Coral Fungi

Coral fungi in Phu Hin Rong Kla National Park, Thailand, were surveyed from June to September of 2011 and 2012. A novel species, Phaeoclavulina pseudozippelii is described together with illustrations, photographs, and a comparison with phenetically similar taxa. Its taxonomic position was confirmed through phylogenetic analyses of the large subunit and internal transcribed spacer (ITS1-5.8S-ITS2) ribosomal DNA sequences.

Multigene differences between Microdochium nivale and Microdochium majus

Botany ◽  
2013 ◽  
Vol 91 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Linda Elizabeth Jewell ◽  
Tom Hsiang
Keyword(s):  
Triticum Aestivum ◽  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Low Temperatures ◽  
Internal Transcribed Spacer ◽  
Elongation Factor ◽  
Microdochium Nivale ◽  
Sister Species ◽  
The North ◽  
Β Tubulin

Microdochium nivale (Fr.) Samuels & Hallett and Microdochium majus (Wollenw.) Glynn & S.G. Edwards are sister species that cause diseases on grasses and cereals at low temperatures. The DNA sequences of RPB2 (RNA polymerase II), β-tubulin, EF-1α (elongation factor), and ITS (rDNA internal transcribed spacer) from these groups were analysed to compare the extent of differences between these species, among isolates from Europe compared with those from North America, and among isolates of M. nivale originally collected from Agrostis spp. compared with isolates from wheat (Triticum aestivum). All of the regions studied except for ITS resolved M. nivale and M. majus isolates into separate clades. The RPB2 sequences also resolved both the North American and European M. majus isolates and M. nivale isolates from either turfgrasses or wheat into separate clades. These results support the recent elevation of M. nivale and M.majus to sister species and also provide some support for the assertion that there may be host-specific differences among M. nivale, which has a wider host range than M. majus.

A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 111
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Central China ◽  
Nuclear Ribosomal Dna ◽  
Light Blue ◽  
Ectal Excipulum ◽  
A New Species

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.

scholarly journals First Report of Musk Thistle Rust (Puccinia carduorum) in California and Nevada

Plant Disease ◽  
2002 ◽  
Vol 86 (7) ◽  
pp. 814-814 ◽  
Author(s):  
D. M. Woods ◽  
M. J. Pitcairn ◽  
D. G. Luster ◽  
W. L. Bruckart
Keyword(s):  
Ribosomal Rna ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
East Coast ◽  
Rust Fungus ◽  
The United States ◽  
Rust Disease ◽  
Carduus Nutans ◽  
Its1 And Its2 ◽  
Spacer Dna

Musk thistle, Carduus nutans L., is an introduced weed of pastures, rangelands, and natural areas in much of North America. Puccinia carduorum Jacky, an autoecious rust fungus from Turkey, has been evaluated for biological control of musk thistle since 1978, including a field study near Blacksburg, VA, from 1987 to 1990. After release of the fungus in Virginia, rusted musk thistle was found in eight eastern states by 1992, in Missouri by 1994 (1), and in Oklahoma by 1997 (2). A rust disease was discovered on musk thistle near Mt. Shasta, CA, on 22 September 1998, and near Mogul, NV, on 12 August 1999. The pathogen was identified as P. carduorum on the basis of pathogenicity on musk thistle and urediniospore morphology (ovate spores, 21 μm diameter, three germ pores equatorial in location, and echinulations over the upper two-thirds to three-quarters of urediniospores). Ribosomal RNA internal transcribed spacer DNA sequences (ITS1 and ITS2) were identical to those from the isolate obtained after the field release in Virginia, verifying that the California isolate is P. carduorum. The initial California infestation was observed on a few plants late in the season, and by September 2000, nearly 100% of plants were infected. The occurrence of P. carduorum in California is apparently the result of natural, unaided spread of the fungus on musk thistle from the East Coast of the United States. References: (1) A. B. A. M. Baudoin and W. L. Bruckart. Plant Dis. 80:1193, 1996. (2) L. J. Littlefield et al. Plant Dis. 82:832, 1998.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus, and relationships within Ophiostoma based on partial ribosomal DNA sequences

1993 ◽  
Vol 71 (9) ◽  
pp. 1249-1265 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Data Set ◽  
Rdna Sequences ◽  
Morphological Criteria ◽  
Small Subunit Rdna ◽  
Rdna Sequence Data ◽  
Large Subunit Rdna

Phylogenetic analysis of partial rDNA sequences suggests that Ophiostoma should remain the sole genus of the Ophiostomataceae, and this should be the sole family within the Ophiostomatales, whereas Ceratocystis s.s. would be best disposed within the Microascales. Although morphological criteria suggest that the genus Ophiostoma is heterogeneous, analysis of partial small subunit rDNA sequence data shows that Ophiostoma (excluding O. roraimense) represents a monophyletic taxon. Analysis of a partial large subunit rDNA data set, which included sequences from 55 species assignable to Ophiostoma, failed to support the strict subdivision of the genus based on either ascospore characters or the nature of the anamorph. Key words: Ceratocystis, Microascus, Ophiostoma, partial rDNA sequences, phylogeny.

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