A new species of salamander (Caudata: Plethodontidae, Bolitoglossa) from Sierra Nevada de Mérida, Venezuela

Zootaxa ◽  
2013 ◽  
Vol 3620 (1) ◽  
pp. 179-191 ◽  
Author(s):  
JAVIER GARCÍA-GUTIÉRREZ ◽  
MOISÉS ESCALONA ◽  
ANDRÉS MORA ◽  
AMELIA DÍAZ DE PASCUAL ◽  
GUSTAVO FERMIN
Keyword(s):  
New Species ◽  
Nucleotide Sequence ◽  
16S Rrna ◽  
Sierra Nevada ◽  
Cloud Forest ◽  
Rrna Gene ◽  
Cytb Gene ◽  
Hind Limbs ◽  
A New Species ◽  
The 16S Rrna Gene

In this article, a new species of salamander of the genus Bolitoglossa (Eladinea) from the cloud forest near La Mucuy in Sierra Nevada de Mérida, Venezuelan Andes, is described. Bolitoglossa mucuyensis sp. nov. differs from all Venezuelan salamanders, except B. orestes, by a larger SVL/TL ratio, and from La Culata salamander B. orestes by a reduced webbing extension of the front and hind limbs. Additionally, B. mucuyensis sp. nov. and B. orestes diverge 3.12% in terms of the nucleotide sequence of the 16S rRNA gene, as previously reported, and in 8.1% for the cytb gene as shown in this study.

A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam

Zootaxa ◽  
2017 ◽  
Vol 4243 (3) ◽  
pp. 544 ◽  
Author(s):  
JODI J. L. ROWLEY ◽  
VINH Q. DAU ◽  
HUY D. HOANG ◽  
DUONG T. T. LE ◽  
TIMOTHY P. CUTAJAR ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Dna Sequences ◽  
Dominant Frequency ◽  
Rrna Gene ◽  
Adult Males ◽  
Skin Texture ◽  
Adult Females ◽  
A New Species ◽  
The 16S Rrna Gene

We describe a new, medium-sized Leptolalax species from Vietnam. Leptolalax petrops sp. nov. is distinguished from its congeners by a combination of having a medium-sized body (23.6–27.6 mm in 21 adult males, 30.3–47.0 mm in 17 adult females), immaculate white chest and belly, no distinct black markings on the head, highly tuberculate skin texture, toes lacking webbing and with narrow lateral fringes, and a call consisting of an average of four notes and a dominant frequency of 5.6–6.4 kHz (at 24.5–25.3 °C). Uncorrected sequence divergences between L. petrops sp. nov. and all homologous DNA sequences available for the 16S rRNA gene are >8%. 

scholarly journals A new species of freshwater crab of the genus Qianguimon Huang, 2018 (Decapoda: Brachyura: Potamidae) from Guangxi, Southern China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9194
Author(s):  
Song-Bo Wang ◽  
Ya-Nan Zhang ◽  
Jie-Xin Zou
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Southern China ◽  
Terminal Segment ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Freshwater Crab ◽  
Autonomous Region ◽  
A New Species ◽  
The 16S Rrna Gene

A new species of freshwater crab of the genus Qianguimon Huang, 2018, is described from Guangxi Zhuang Autonomous Region, southern China. It can be distinguished from congeners by the following characters: male first gonopods bent inward at about 45° at base of terminal segment, carapace regions distinct and rugged and the female vulva opening inwards and downwards. In addition, molecular evidence derived from the 16S rRNA gene supported the species described in this study as a new species of Qianguimon.

A new species of the genus Leptochela (Decapoda, Caridea, Pasiphaeidae) from the Yellow Sea

Crustaceana ◽  
2017 ◽  
Vol 90 (7-10) ◽  
pp. 1267-1277 ◽  
Author(s):  
Yaqin Wang ◽  
Zhibin Gan ◽  
Xinzheng Li
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Yellow Sea ◽  
Systematic Position ◽  
The Yellow Sea ◽  
Morphological Data ◽  
Rrna Gene ◽  
Distal Margin ◽  
A New Species ◽  
The 16S Rrna Gene

Based on samples collected from the Yellow Sea in October 2014, a new species of pasiphaeid shrimp, Leptochela (Leptochela) tuerkayi sp. nov., is described. The new species is closely related to L. (L.) gracilis Stimpson, 1860 in morphology but differs from the latter and other congeners by the following combination of characters: three longitudinal dorsal carinae in ovigerous females, third maxilliped with the rather short exopod slightly overreaching the distal margin of the antepenultimate segment and the fourth pereiopod with the exopod reaching beyond the end of the merus. Morphological data and a partial sequence of the 16S rRNA gene of the new species are provided to show its systematic position.

scholarly journals A new frog of the Eleutherodactylus abbotti species group (Anura: Eleutherodactylidae) from Hispaniola, with bioacoustic and taxonomic comments on other species

2018 ◽  
pp. 25-42
Author(s):  
Luis M. Díaz ◽  
Sixto J. Incháustegui ◽  
Cristian Marte ◽  
Gunther Köhler ◽  
Antonio Cádiz ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Dna Sequences ◽  
Taxonomic Status ◽  
Genetic Data ◽  
Species Group ◽  
Rrna Gene ◽  
Cordillera Central ◽  
A New Species ◽  
The 16S Rrna Gene

A new species of frog, Eleutherodactylus geitonos sp. nov., is described from the southeastern slope of the Cordillera Central, Dominican Republic. The new frog is closely related to E. haitianus and both species share a small size. They differ in some morphological proportions and other external features, coloration, advertisement calls and DNA sequences of the 16S rRNA gene. Morphological and bioacoustic comparisons with other species in the  E. abbotti species group are also provided. Our preliminary genetic data suggest that the taxonomic status of E. neodreptus (a synonym of E. audanti) and E. melatrigonum need to be re-evaluated.

A new species of Puddle Frog, genus Phrynobatrachus (Amphibia: Anura: Phrynobatrachidae) from Ghana

Zootaxa ◽  
2018 ◽  
Vol 4374 (4) ◽  
pp. 565 ◽  
Author(s):  
CALEB OFORI-BOATENG ◽  
ADAM D. LEACHÉ ◽  
BRIGHT OBENG-KANKAM ◽  
N’GORAN GERMAIN KOUAMÉ ◽  
ANNIKA HILLERS ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
New Species ◽  
Sequence Data ◽  
Face Mask ◽  
West African ◽  
Rrna Gene ◽  
Forest Region ◽  
Mtdna Sequence ◽  
A New Species ◽  
The 16S Rrna Gene

We describe a new species of Phrynobatrachus from the eastern part of the Upper Guinea forest region, Ghana, West Africa. Morphologically, the new species can be distinguished from all of its congeners by the combination of a slender body, short and pointed snout, a relatively warty dorsum, a black-spotted throat in both sexes, a gular flap in males, a dark spotted chest, a white-greyish venter with occasional blackish spots, rudimentary pedal webbing, none to slightly dilated finger tips and strongly delated toe tips, presence of both inner and outer metatarsal tubercles and absence of a dark face mask, eyelid tubercles and longer dorsal ridges. We collected mitochondrial DNA (mtDNA) sequence data from the 16S rRNA gene to measure the genetic diversity of the new species, and to estimate phylogenetic relationships. The new species is a distinct and monophyletic evolutionary lineage most closely related to Phrynobatrachus gutturosus, P. fraterculus and P. maculiventris. The discovery of this new species highlights that the biodiversity of West African forests is still incompletely known and that the few remaining forests need urgent protection. 

Two additional treefrogs of the Boophis ulftunni species group (Anura: Mantellidae) discovered in rainforests of northern and south-eastern Madagascar

Zootaxa ◽  
2008 ◽  
Vol 1814 (1) ◽  
pp. 37 ◽  
Author(s):  
JÖRN KÖHLER ◽  
FRANK GLAW ◽  
MIGUEL VENCES
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Ovigerous Female ◽  
Species Group ◽  
Rrna Gene ◽  
Threat Status ◽  
Frog Species ◽  
Southern Central ◽  
Phylogenetic Affinities ◽  
The 16S Rrna Gene

We describe two new frog species of the endemic Malagasy-Comoroan genus Boophis. One species, described as Boophis baetkei sp. n., originates from Forêt d'Ambre Special Reserve in northernmost Madagascar, whereas Boophis lilianae sp. n. was discovered near Ifanadiana and Ranomafana in the Southern Central East of the island. Both new species have very deep genetic divergences in the 16S rRNA gene that complicate the assessment of their phylogenetic affinities but are here tentatively assigned to the recently defined Boophis ulftunni species group based on phenetic similarity and preliminary results of analyses of other genes. All three species known in this group share a green dorsum with translucent shade in life, a pigmented venter and, most characteristic, pink markings in life and in preservative. Boophis lilianae sp. n. is the smallest species of Boophis known so far (SVL of adult male 18.3 mm, ovigerous female 20.0 mm). Phylogenetic relationships, distribution and threat status of the new species are discussed.

scholarly journals Ixodes kangdingensis (Acari: Ixodidae), a new species from the Siberian weasel, Mustela sibirica (Carnivora: Mustelidae) in China

2017 ◽  
Vol 3 ◽  
Author(s):  
TIANYU GUO ◽  
YI SUN ◽  
GUANG XU ◽  
LANCE A. DURDEN
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Divergence ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Ixodes Arboricola ◽  
A New Species

SUMMARY The female and nymph of Ixodes (Pholeoixodes) kangdingensis n. sp. are described based on both morphology and analysis of 16S rRNA gene sequences. Specimens of this new tick species were collected from a Siberian weasel (Mustela sibirica) in Kangding County, Sichuan Province, China. The morphological features of the female and nymph are unique to distinguish I. kangdingensis n. sp. from other members of the subgenus Pholeoixodes, including the presence of distinctly shaped cornua, anterior and posterior processes on palpal article I and a large angular projection on each side of the hypostome. Partial sequence of 16S rRNA gene grouped this species with Ixodes arboricola and Ixodes lividus with sequence divergence of new species from I. arboricola 4·16% and from I. lividus 8·49%. Data on the phylogenetic position, hosts, geographic distribution and key to females of closely related species are also provided.

Molecular taxonomic reassessment of the Cloud Forest’s Bolitoglossa salamanders (Caudata: Plethodontidae) from Cordillera de Mérida (Mérida state, Venezuela)

Zootaxa ◽  
2012 ◽  
Vol 3356 (1) ◽  
pp. 47 ◽  
Author(s):  
GUSTAVO FERMIN ◽  
JAVIER GARCÍA-GUTIÉRREZ ◽  
MOISÉS ESCALONA ◽  
ANDRÉS MORA ◽  
AMELIA DÍAZ
Keyword(s):  
16S Rrna ◽  
Sierra Nevada ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Morphological Evidence ◽  
Rrna Gene ◽  
Putative Species ◽  
Salamander Species ◽  
The 16S Rrna Gene ◽  
Tissue Digestion

Salamanders found at different localities nearby Mérida city, Venezuela, are thus far reported as Bolitoglossa orestes or B.spongai. However, morphological ambiguities among individuals from several populations of both putative species, besidestheir reported disparate geographical distributions, prompted us to clarify the specific identity of these bolitoglossines throughthe sequence analysis of their corresponding 16S rRNA genes. Seventeen specimens belonging to the vertebrates collection ofUniversidad de Los Andes (CVULA), collected at separated cloud forests in Sierra La Culata (San Eusebio, Macho Capaz andSan Javier del Valle) and Sierra Nevada de Mérida (La Mucuy), were used to extract DNA upon tissue digestion. Sequenceanalysis of the 16S rRNA gene supports a biogeographical scenario where, so far, there is only one salamander species for eachsierra: B. orestes, which is widely distributed in Sierra La Culata, and a so far undescribed species of a Venezuelan bolitogloss-ine apparently restricted to Sierra Nevada de Mérida. Based on our molecular results and an examination of morphological evidence, B. spongai should be considered a synonym of B. orestes.

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