A new species of Puddle Frog, genus Phrynobatrachus (Amphibia: Anura: Phrynobatrachidae) from Ghana

Zootaxa ◽  
2018 ◽  
Vol 4374 (4) ◽  
pp. 565 ◽  
Author(s):  
CALEB OFORI-BOATENG ◽  
ADAM D. LEACHÉ ◽  
BRIGHT OBENG-KANKAM ◽  
N’GORAN GERMAIN KOUAMÉ ◽  
ANNIKA HILLERS ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
New Species ◽  
Sequence Data ◽  
Face Mask ◽  
West African ◽  
Rrna Gene ◽  
Forest Region ◽  
Mtdna Sequence ◽  
A New Species ◽  
The 16S Rrna Gene

We describe a new species of Phrynobatrachus from the eastern part of the Upper Guinea forest region, Ghana, West Africa. Morphologically, the new species can be distinguished from all of its congeners by the combination of a slender body, short and pointed snout, a relatively warty dorsum, a black-spotted throat in both sexes, a gular flap in males, a dark spotted chest, a white-greyish venter with occasional blackish spots, rudimentary pedal webbing, none to slightly dilated finger tips and strongly delated toe tips, presence of both inner and outer metatarsal tubercles and absence of a dark face mask, eyelid tubercles and longer dorsal ridges. We collected mitochondrial DNA (mtDNA) sequence data from the 16S rRNA gene to measure the genetic diversity of the new species, and to estimate phylogenetic relationships. The new species is a distinct and monophyletic evolutionary lineage most closely related to Phrynobatrachus gutturosus, P. fraterculus and P. maculiventris. The discovery of this new species highlights that the biodiversity of West African forests is still incompletely known and that the few remaining forests need urgent protection. 

A new species of trypanosome, Trypanosoma desterrensis sp. n., isolated from South American bats

Parasitology ◽  
2003 ◽  
Vol 127 (3) ◽  
pp. 265-271 ◽  
Author(s):  
E. C. GRISARD ◽  
N. R. STURM ◽  
D. A. CAMPBELL
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Rrna Gene ◽  
South American ◽  
Sequence Variability ◽  
Dna Sequence Data ◽  
Hybridization Probes ◽  
5S Rrna Gene ◽  
A New Species

Trypanosomes isolated from South American bats include the human pathogen Trypanosoma cruzi. Other Trypanosoma spp. that have been found exclusively in bats are not well characterized at the DNA sequence level and we have therefore used the SL RNA gene to differentiate and characterize kinetoplastids isolated from bats in South America. A Trypanosoma sp. isolated from bats in southern Brazil was compared with the geographically diverse isolates T. cruzi marinkellei, T. vespertilionis, and T. dionisii. Analysis of the SL RNA gene repeats revealed size and sequence variability among these bat trypanosomes. We have developed hybridization probes to separate these bat isolates and have analysed the DNA sequence data to estimate their relatedness. A new species, Trypanosoma desterrensis sp. n., is proposed, for which a 5S rRNA gene was also found within the SL RNA repeat.

A new species of salamander (Caudata: Plethodontidae, Bolitoglossa) from Sierra Nevada de Mérida, Venezuela

Zootaxa ◽  
2013 ◽  
Vol 3620 (1) ◽  
pp. 179-191 ◽  
Author(s):  
JAVIER GARCÍA-GUTIÉRREZ ◽  
MOISÉS ESCALONA ◽  
ANDRÉS MORA ◽  
AMELIA DÍAZ DE PASCUAL ◽  
GUSTAVO FERMIN
Keyword(s):  
New Species ◽  
Nucleotide Sequence ◽  
16S Rrna ◽  
Sierra Nevada ◽  
Cloud Forest ◽  
Rrna Gene ◽  
Cytb Gene ◽  
Hind Limbs ◽  
A New Species ◽  
The 16S Rrna Gene

In this article, a new species of salamander of the genus Bolitoglossa (Eladinea) from the cloud forest near La Mucuy in Sierra Nevada de Mérida, Venezuelan Andes, is described. Bolitoglossa mucuyensis sp. nov. differs from all Venezuelan salamanders, except B. orestes, by a larger SVL/TL ratio, and from La Culata salamander B. orestes by a reduced webbing extension of the front and hind limbs. Additionally, B. mucuyensis sp. nov. and B. orestes diverge 3.12% in terms of the nucleotide sequence of the 16S rRNA gene, as previously reported, and in 8.1% for the cytb gene as shown in this study.

A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam

Zootaxa ◽  
2017 ◽  
Vol 4243 (3) ◽  
pp. 544 ◽  
Author(s):  
JODI J. L. ROWLEY ◽  
VINH Q. DAU ◽  
HUY D. HOANG ◽  
DUONG T. T. LE ◽  
TIMOTHY P. CUTAJAR ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Dna Sequences ◽  
Dominant Frequency ◽  
Rrna Gene ◽  
Adult Males ◽  
Skin Texture ◽  
Adult Females ◽  
A New Species ◽  
The 16S Rrna Gene

We describe a new, medium-sized Leptolalax species from Vietnam. Leptolalax petrops sp. nov. is distinguished from its congeners by a combination of having a medium-sized body (23.6–27.6 mm in 21 adult males, 30.3–47.0 mm in 17 adult females), immaculate white chest and belly, no distinct black markings on the head, highly tuberculate skin texture, toes lacking webbing and with narrow lateral fringes, and a call consisting of an average of four notes and a dominant frequency of 5.6–6.4 kHz (at 24.5–25.3 °C). Uncorrected sequence divergences between L. petrops sp. nov. and all homologous DNA sequences available for the 16S rRNA gene are >8%. 

scholarly journals A new species of Proceratophrys Miranda-Ribeiro, 1920 (Anura, Odontophrynidae) from Southern Amazonia, Brazil

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12012
Author(s):  
Diego J. Santana ◽  
Leandro Alves da Silva ◽  
Anathielle Caroline Sant’Anna ◽  
Donald B. Shepard ◽  
Sarah Mângia
Keyword(s):  
New Species ◽  
Distinct Species ◽  
Integrative Approach ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Hydroelectric Dams ◽  
Mato Grosso ◽  
Southern Amazonia ◽  
A New Species ◽  
The 16S Rrna Gene

Based on concordant differences in morphology, male advertisement call, and 16S mtDNA barcode distance, we describe a new species of Proceratophrys from southern Amazonia, in the states of Mato Grosso and Pará, Brazil. The new species is most similar to P. concavitympanum and P. ararype but differs from these species by its proportionally larger eyes and features of the advertisement call. Additionally, genetic distance between the new species and its congeners is 3.0–10.4% based on a fragment of the 16S rRNA gene, which is greater than the threshold typically characterizing distinct species of anurans. Using an integrative approach (molecular, bioacoustics, and adult morphology), we were able to distinguish the new species from other congeneric species. The new species is known only from the type locality where it is threatened by illegal logging and gold mining as well as hydroelectric dams.

scholarly journals A new species of planthopper from Costa Rica in the genus Oropuna from palms in lowland tropical rainforest

Zootaxa ◽  
2021 ◽  
Vol 5081 (1) ◽  
pp. 116-130
Author(s):  
BRIAN W. BAHDER ◽  
MARCO A. ZUMBADO ECHAVARRIA ◽  
EDWIN A. BARRANTES BARRANTES ◽  
ERICKA E. HELMICK ◽  
CHARLES R. BARTLETT
Keyword(s):  
New Species ◽  
Costa Rica ◽  
18S Rrna ◽  
Tropical Rainforest ◽  
Sequence Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
The Novel ◽  
Fourth Species ◽  
A New Species

The derbid genus Oropuna is a small taxon of Neotropical planthoppers in the tribe Cenchreini comprised of three species. Recent survey work on palms for planthoppers in Costa Rica resulted in the discovery of a fourth species, Oropuna halo sp. n. In this study the new species is described and a key to the four species is provided along with sequence data for the cyctochrome c oxidase subunit I (COI) and 18S rRNA gene for the novel taxon.  

Review of the genus Cruziohyla (Anura: Phyllomedusidae), with description of a new species

Zootaxa ◽  
2018 ◽  
Vol 4450 (4) ◽  
pp. 401 ◽  
Author(s):  
ANDREW R. GRAY
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Dna Sequences ◽  
Integrated Approach ◽  
Molecular Data ◽  
Rrna Gene ◽  
Phylogenetic Information ◽  
Morphological And Molecular Data ◽  
A New Species ◽  
The 16S Rrna Gene

The presented work summarises new and existing phenotypic and phylogenetic information for the genus Cruziohyla. Data based on morphology and skin peptide profiling supports the identification of a separate new species. Specimens of Cruziohyla calcarifer (Boulenger, 1902) occurring in Ecuador, Colombia, two localities in Panama, and one in the south east Atlantic lowlands of Costa Rica, distinctly differ from those occurring along the Atlantic versant of Central America from Panama northwards through Costa Rica, Nicaragua, to Honduras. A new species—Cruziohyla sylviae sp. n.—(the type locality: Alto Colorado in Costa Rica)—is diagnosed and described using an integrated approach from morphological and molecular data. Phylogenetic analysis of DNA sequences of the 16S rRNA gene confirms the new species having equal minimum 6.2% genetic divergence from both true C. calcarifer and Cruziohyla craspedopus. 

scholarly journals A new species of freshwater crab of the genus Qianguimon Huang, 2018 (Decapoda: Brachyura: Potamidae) from Guangxi, Southern China

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9194
Author(s):  
Song-Bo Wang ◽  
Ya-Nan Zhang ◽  
Jie-Xin Zou
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Southern China ◽  
Terminal Segment ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Freshwater Crab ◽  
Autonomous Region ◽  
A New Species ◽  
The 16S Rrna Gene

A new species of freshwater crab of the genus Qianguimon Huang, 2018, is described from Guangxi Zhuang Autonomous Region, southern China. It can be distinguished from congeners by the following characters: male first gonopods bent inward at about 45° at base of terminal segment, carapace regions distinct and rugged and the female vulva opening inwards and downwards. In addition, molecular evidence derived from the 16S rRNA gene supported the species described in this study as a new species of Qianguimon.

scholarly journals Description of Hydrogenophaga Crocea Sp. Nov. Associated With Cyanobacterial Mat Isolated From Farmland Mud

2021 ◽  
Author(s):  
Vipool Thorat ◽  
Bhavesh Tiwarekar ◽  
Kiran Kirdat ◽  
Saurabhee Chavan ◽  
Shital Marakad ◽  
...  
Keyword(s):  
Fatty Acids ◽  
New Species ◽  
Type Strain ◽  
Sequence Similarity ◽  
Distinct Pattern ◽  
Rrna Gene ◽  
Cyanobacterial Mat ◽  
Positive Strain ◽  
A New Species ◽  
The 16S Rrna Gene

Abstract A catalase and oxidase-positive strain BA0156T was isolated from a cyanobacterial mat collected from the farmland mud cultivated with sugarcane from Ahmednagar, India. The 16S rRNA gene of strain BA0156T showed the highest percent sequence similarity with Hydrogenophaga borbori LMG 30805T (98.55 %), followed by H. flava DSM 619T (98.35 %) and H. intermedia DSM 5680T (98.21 %). The strain BA0156T consisted of major fatty acids, C16:0 (25.1 %) and C17:0 cyclo (3.9 %), whereas phosphatidylethanolamine and diphosphatidylglycerol were the major polar lipids. The OrthoANI and dDDH values between strain BA0156T and its closest relative H. borbori LMG 30805T were 84.59 % and 28.3 %, respectively. The DNA G+C content of strain BA0156T was 69.42 mol %. Furthermore, the biochemical and physiological features of strain BA0156T showed a distinct pattern from their closest phylogenetic neighbours. The phenotypic, genotypic and chemotaxonomic characteristics indicated that the strain BA0156T represents a new species for which the name Hydrogenophaga crocea (type strain BA0156 T = MCC 3062T = KCTC 72452T = JCM 34507T) is proposed.

scholarly journals Phylogenetic position reevaluation of Kyrtuthrix and description of a new species K. huatulcensis from Mexico´s Pacific coast

Phytotaxa ◽  
2016 ◽  
Vol 278 (1) ◽  
pp. 1 ◽  
Author(s):  
HILDA P. LEÓN-TEJERA ◽  
LAURA GONZÁLEZ-RESENDIZ ◽  
JEFFREY R. JOHANSEN ◽  
CLAUDIA SEGAL-KISCHINEVSKY ◽  
VIVIANA ESCOBAR ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Data ◽  
Geographical Information ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Structure Comparison ◽  
A New Species

Benthic marine heterocytous cyanoprokaryotes of Mexico´s tropical coast are being recognized as an important and conspicuous component of the supralittoral and intertidal zones usually described as an extreme and low diversity biotope. Although Kyrtuthrix has been reported from different coasts worldwide, its complex morphology has led to differing taxonomic interpretations and positioning. Ten marine supra and intertidal populations of Kyrtuthrix were analyzed using a detailed morphological approach, complemented with ecological and geographical information as well as DNA sequence data of the 16S rRNA gene and associated 16S–23S ITS. Kyrtuthrix huatulcensis is described as a new species, different from K. dalmatica Ercegovic and K. maculans (Gomont) Umezaki based primarily on morphological data. Our material has smaller dimensions in thalli, filaments, trichomes and cells, and possesses differences in qualitative characters as well. Our material is epilithic as in K. maculans but not endolithic as in K. dalmatica. The analysis of molecular data, mainly 16S rRNA gene sequence, show that this genus is within the Rivulariaceae clade, and outside of the Scytonemataceae clade. ITS secondary structure comparison with some of the closer representatives of Rivulariaceae shows considerable differences with these related genera. Based on the evidence gathered in this study, inclusion of Kyrtuthrix within the Rivulariaceae, rather than the Scytonemataceae, is fully supported.

A new species of the genus Leptochela (Decapoda, Caridea, Pasiphaeidae) from the Yellow Sea

Crustaceana ◽  
2017 ◽  
Vol 90 (7-10) ◽  
pp. 1267-1277 ◽  
Author(s):  
Yaqin Wang ◽  
Zhibin Gan ◽  
Xinzheng Li
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Yellow Sea ◽  
Systematic Position ◽  
The Yellow Sea ◽  
Morphological Data ◽  
Rrna Gene ◽  
Distal Margin ◽  
A New Species ◽  
The 16S Rrna Gene

Based on samples collected from the Yellow Sea in October 2014, a new species of pasiphaeid shrimp, Leptochela (Leptochela) tuerkayi sp. nov., is described. The new species is closely related to L. (L.) gracilis Stimpson, 1860 in morphology but differs from the latter and other congeners by the following combination of characters: three longitudinal dorsal carinae in ovigerous females, third maxilliped with the rather short exopod slightly overreaching the distal margin of the antepenultimate segment and the fourth pereiopod with the exopod reaching beyond the end of the merus. Morphological data and a partial sequence of the 16S rRNA gene of the new species are provided to show its systematic position.

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