Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 709-723 ◽  
Author(s):  
Zhen Wang ◽  
Wim Bert ◽  
Jianfeng Gu ◽  
Marjolein Couvreur ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Medicago Sativa ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male Tail ◽  
The Usa ◽  
Group 3 ◽  
A New Species

Summary Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μm long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μm long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis.

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Description of Stegelletina lingulata sp. n. (Nematoda, Rhabditida, Cephalobidae) from xeric environments in Iran

Zootaxa ◽  
2017 ◽  
Vol 4358 (3) ◽  
pp. 462
Author(s):  
JOAQUÍN ABOLAFIA ◽  
EBRAHIM SHOKOOHI
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Small Body ◽  
Reproductive Systems ◽  
Lateral Field ◽  
Male Tail ◽  
Line Drawings ◽  
Female Tail ◽  
Body Diameter ◽  
A New Species

A new species belonging to the genus Stegelletina, S. lingulata sp. n., is described from xeric natural areas from Iran. It is characterized by having small body (0.51–0.69 mm in females and 0.45–0.64 mm in males), cuticle slightly tessellated especially at anterior end, lateral field with three longitudinal incisures, lips with long acute process at primary axils and three more dentate processes at secondary axils, primary axils with one triangular axillar guard process and secondary axils lacking axillar processes, labial probolae long (9–11 µm) with slender peduncle lacking adaxial expansion and with convergent smooth prongs, stoma, pharynx and reproductive systems cephaloboid, excretory pore at isthmus level, spermatheca 1.1–1.4 times, and post-vulval sac 0.9–1.2 times the corresponding body diameters in length, female rectum 0.6–0.8 times anal body diameter long, female tail conoid (35–47 µm long, c = 14.0–15.6, c' = 2.1–2.7) with fine rounded terminus, male tail conoid, curved ventrad (53–59 µm long, c = 11.0–12.0, c' = 1.9–2.5) with rounded terminus, spicules 31–33 µm long with ventrally straight manubrium and gubernaculum 14–17 µm long. Measurements and illustrations (line drawings, LM and SEM) are provided for this species. In addition, comments on the morphology of the lip region of the species of the genus Stegelletina are included. 

Bursaphelenchus moensi n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from the USA

Nematology ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 133-146 ◽  
Author(s):  
Xuan Wang ◽  
Munawar Maria ◽  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Jincheng Wang ◽  
...  
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Gene Sequencing ◽  
Nerve Ring ◽  
Central Projection ◽  
Closely Related Species ◽  
Lateral Field ◽  
The Usa ◽  
Pointed End ◽  
Partial 18S

Bursaphelenchusmoensin. sp., isolated in Tianjin Port, China, fromPinuspackaging wood imported from the USA, is described. It is characterised by a lateral field with three lines (two bands), stylet 13.0-14.9μm long, excretory pore situated at same level as, or slightly posterior to, the nerve ring, spicules 13.0-15.5μm long, mitten-shaped, condylus high and broad with squared, rounded or pointed end, rostrum triangular or conical with bluntly rounded tip, and cucullus absent, bursa small, starting posterior to P4, narrow oblong to irregular in shape and with an oval central projection, female vulval flap absent, and tail conical with a finely rounded or mucronate terminus. The new species belongs to thehofmanni-group and is most similar toB. paraparvispicularis,B. parvispicularisandB. wuae. It is distinguished from closely related species by morphology, ITS-RFLP patterns and partial 18S, ITS and 28S D2-D3 rDNA gene sequencing results.

Description of Ruehmaphelenchus taedae n. sp. (Nematoda: Aphelenchoididae) found in Loblolly pine logs from the USA

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Xiaoling Lü ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Loblolly Pine ◽  
Excretory Pore ◽  
Lateral Field ◽  
Female Tail ◽  
The Status ◽  
The Usa ◽  
Males And Females ◽  
A New Species

Summary Ruehmaphelenchus taedae n. sp., isolated from Loblolly pine logs (Pinus taedae L.) from the USA, is described and figured. It is characterised by a relatively slim body (a = 42 and 43 for males and females, respectively), three lines in the lateral field, male spicules relatively small (12-18 μm) with high and dorsally bent condylus and weakly developed rostrum, bursal flap absent, short tail possessing a long terminal spike ending in a bluntly rounded tip and 8.7-13.3 μm long, vulva positioned at ca 83% of body length, vulval flap absent, vulval lips slightly protruding, post-vulval uterine branch extending for less than half of vulva to anus distance, and female tail conoid, ca 3-4 anal body diam. long, with 13.7-18.5 μm terminal projection. The new species can be separated from all other species of the genus by the male tail possessing a long terminal spike and the more anterior excretory pore. Detailed phylogenetic analysis based on 28S D2-D3 region sequences confirmed the status of this nematode as a new species.

Bursaphelenchus paraleoni n. sp. (Tylenchina: Aphelenchoididae) intercepted in Loblolly pine logs imported into China from the USA

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Xinxin Ma ◽  
Xiaoling Lu
Keyword(s):  
New Species ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Precloacal Papilla ◽  
Long Tail ◽  
Lateral Field ◽  
The Usa ◽  
Males And Females ◽  
A New Species ◽  
Huge Variation

Summary Bursaphelenchus paraleoni n. sp. was isolated from Loblolly pine, Pinus taeda, logs from the USA. The new species is characterised by a lateral field with three lines, 13-16 μm long stylet with small basal swellings, and the excretory pore located slightly posterior to the nerve ring. The male spicules are mitten-shaped, the lamina smoothly tapering towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular or conical with finely rounded tip, and cucullus absent. One midventral precloacal papilla, one adcloacal pair and two postcloacal pairs of genital papillae are present. The bursal flap is long and spade-like with the posterior margin truncate or irregular. Females have a small vulval flap 4-6 μm long, a post-vulval uterine sac extending for 34-49% of the vulva to anus distance, a very long tail with slight ventral curvature, mostly filiform and with a finely rounded terminus, occasionally bluntly rounded. Huge variation in the tails of males and females were observed in a culture. It is similar to species of B. eidmanni, B. leoni, and B. silvestris in the leoni-group but detailed phylogenetic analysis based on 18S, ITS and D2-D3 28S sequences confirmed it as a new species.

A new species of Aoruroides Travassos & Kloss, 1958 (Nematoda: xyuridomorpha: Thelastomatidae) parasite of the wood-burrowing cockroach Panesthia angustipennis spadica (Shiraki, 1906) (Blattodea: Blaberidae: Panesthiinae) from Japan with comments on the validity of the genus Aoruroides

Zootaxa ◽  
2019 ◽  
Vol 4712 (3) ◽  
pp. 365-376
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KOICHI HASEGAWA ◽  
RAMON A. CARRENO
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Nerve Ring ◽  
The Body ◽  
Morphological Data ◽  
Lsu Rdna ◽  
Japanese Archipelago ◽  
The Japanese Archipelago ◽  
A New Species

Aoruroides chubudaigaku n. sp. (Nematoda: Oxyuridomorpha: Thelastomatidae) is described from the wood-burrowing cockroach Panesthia angustipennis spadica (Shiraki, 1906) (Blattodea: Blaberidae: Panesthiinae) from Aichi prefecture, Japan. Females of A. chubudaigaku n. sp. are similar to A. costaricensis Carreno & Tuhela, 2011 by the position of the nerve ring at level of the first third of the isthmus, the body length and the comparative measurements of the oesophagus and tail. They differ by the position of the excretory pore and the vulva. The males of A. chubudaigaku n. sp. are characterized by the absence of ornamentations in the cervical cuticle and the nerve ring located at the posterior third of the corpus. In addition, the males of the new species can be differentiated by the length of the body and the comparative lengths of the oesophagus and the tail. The phylogeny of A. chubudaigaku n. sp. is inferred by the D2-D3 domains of the LSU rDNA and the validity of the genus Aoruroides Travassos & Kloss, 1958 is discussed on the basis of both molecular and morphological data. This constitutes the first species of the genus Aoruroides described from the Japanese archipelago. 

Bursaphelenchus pterocarpi n. sp. (Tylenchina: Aphelenchoididae) found in Pterocarpus sp. imported into China from Ghana

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 725-737
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Majid Pedram ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Reproductive System ◽  
Female Body ◽  
Nerve Ring ◽  
Wild Type ◽  
Lateral Field ◽  
Group Species ◽  
Partial 18S

Summary Bursaphelenchus pterocarpi n. sp. isolated from Pterocarpus sp. logs imported from Ghana to Ningbo, China, is described and illustrated. The new species is characterised by a female body length of 630-946 μm, lateral field with four lines, a short 12.6-13.9 μm long stylet with small basal swellings, excretory pore located posterior to nerve ring, outstretched reproductive system with amoeboid sperm inside the spermatheca, a well-developed vulval flap, tail conical and straight with a 1.9-4.8 μm long mucron at the tip, and lacking males in both the wild type and fungal-cultured populations. In a concatenated tree, reconstructed on the basis of the partial 18S, full length ITS and partial 28S rDNA D2-D3 sequences, the new species occupied a basal placement to the clade of xylophilus-group and africanus-group species.

Bursaphelenchus parayongensis n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from China

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Ute Schönfeld ◽  
Xinxin Ma ◽  
Xiaoling Lü
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Posterior Part ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
The Status ◽  
A New Species ◽  
Partial 18S

Summary Bursaphelenchus parayongensis n. sp. was isolated from packaging wood of a consignment from China exported to Germany. The new species is characterised by the lateral field with three lines, 9-13 μm long stylet with small basal swellings, and the excretory pore located near the posterior part of metacorpus. The male spicules are mitten-shaped, the lamina smoothly tapers towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular with sharply pointed tip, and without cucullus. One precloacal papilla, one adcloacal pair and two postcloacal pairs of male papillae present, with the posteriormost pair forming ‘gland papillae’. Bursal flap long, spade-like with posterior margin truncate or irregular. Females have a small vulval flap of 2-3 μm long, and a post-vulval uterine sac extending for 55-77% of the vulva to anus distance. Tail cylindrical, mostly (about 70%) with a robust mucron, very short to less than 3 μm, but occasionally tail terminus bluntly rounded, without mucron. It is similar to species in subgroup 3 of the eggersi group including B. carpini, B. clavicauda, B. cryphali and B. yongensis, but morphologically different. Detailed phylogenetic analysis based on partial 18S, ITS and D2-D3 region of 28S sequences has confirmed the status of this nematode as a new species.

Radopholus duriophilus sp. n. (Nematoda: Pratylenchidae) from Western Highland of Vietnam

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 549-558 ◽  
Author(s):  
Chau Nguyen ◽  
Sergei Subbotin ◽  
Mehrdad Madani ◽  
Phap Trinh ◽  
Maurice Moens
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Sequence Divergence ◽  
Its Sequence ◽  
Entire Body ◽  
Lateral Field ◽  
Stylet Length ◽  
High Level ◽  
A New Species ◽  
Oval Shape

Abstract A new species of the genus Radopholus associated with durian (Durio zibetinus M.) in the Western Highland of Vietnam is described as Radopholus duriophilus sp. n. The new species is close to R. similis, but is distinguished from R. similis by the position of the excretory pore located posterior to pharyngo-intestine junction (vs at level of pharyngo-intestine junction), oval shape sperm (vs rod-like), four incisures terminating far behind position of phasmid (vs three incisures terminating at or just behind phasmid), and bursa in male never reaching tail terminus (vs bursa reaching tail terminus). Females of R. duriophilus sp. n. differ from R. nativus females by stylet length (16.5-19 vs 19-23 μm), oval or kidney-shaped sperm (vs rod-like), four incisures at level of phasmid (vs three) and their areolated lateral field (vs not areolated). The position of excretory pore of both female and male is located posterior to pharyngo-intestine junction (vs at level or anterior to pharyngo-intestine junction). Females of R. duriophilus sp. n. differ from R. clarus females by stylet length (16.5-19 vs 19-21 μm) and areolated lateral field (vs no areolation). Females of R. duriophilus sp. n. differ from R. musicola females by their lateral field with equidistant incisures at mid-body (vs two deep outer folds and two faint shallow inner incisures), oval or kidney-shaped sperm (vs rod-like), and rounded terminus tail (vs sharply pointed). The species also sp. n. differ in male stylet length (11.5-15 vs 8.8-12 μm). Females of R. duriophilus sp. n. differ from R. bridgei females by stylet length (16.5-19 vs 15-17.5 μm), median bulb length (11-16.5 vs 11-13 μm), length of hyaline tail (3-11 vs not more than 4 μm), and lateral field areolated for entire body (vs not areolated except irregularly on neck and tail). The male differs by stylet length (11.5-15 vs 10-12 μm) and length of the hyaline portion (4-9 vs 1-4 μm). In addition, the relatively high level of ITS sequence divergence of the new species from R. similis populations and the presence of nucleotide autapomorphies support a separate specific status for these durian populations. Results of surveys revealed that R. duriophilus sp. n. is rather widely distributed in durian orchards and associated with decline and death of trees in many durian nursery gardens. Densities of nematode population reached thousands of individuals per g of root samples.

Aphelenchoides smolae n. sp. (Tylenchina: Aphelenchoididae) found in Lilium orientalis imported into China from The Netherlands

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 799-813
Author(s):  
Jingjing Cai ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Yiwu Fang ◽  
Hongmei Li
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
The Netherlands ◽  
Body Length ◽  
Excretory Pore ◽  
Female Body ◽  
Nerve Ring ◽  
Male Body ◽  
Lateral Field ◽  
Group 2

Summary Aphelenchoides smolae n. sp. isolated in Ningbo Port, China, from medium soil and tissues of Lilium orientalis bulbs imported from The Netherlands, is described. It is characterised by the female body length of 826 (672-1002) μm and male body length of 785 (683-844) μm, lateral field with four lines (three bands), stylet 13.0-14.9 μm long with small basal swellings, excretory pore situated slightly posterior to nerve ring, vulval flap absent, post-vulval uterine sac well developed and extending for ca 35.7-62.4% of vulva to anus distance, conical tail with terminus bearing a single mucron with many tiny nodular protuberances under SEM, males common, with spicules 25.7-29.8 μm long (median curved line), the condylus and rostrum rounded, well developed, the dorsal limb with a hook-like tip, and tail bearing three pairs (2 + 2 + 2) of caudal papillae and a sharply pointed mucron. The new species belongs to the Group 2 category of Aphelenchoides species sensu Shahina. Phylogenetic analysis based on 18S, 28S D2-D3 of rDNA and COI mtDNA further characterised the new species.

Sign in / Sign up

Export Citation Format

Share Document