Green light to an integrative view of Microscolex phosphoreus (Dugès, 1837) (Annelida: Clitellata: Acanthodrilidae)

Zootaxa ◽  
2018 ◽  
Vol 4496 (1) ◽  
pp. 175 ◽  
Author(s):  
EMILIA ROTA ◽  
SVANTE MARTINSSON ◽  
CHRISTER ERSÉUS ◽  
VALENTIN N. PETUSHKOV ◽  
NATALJA S. RODIONOVA ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Green Light ◽  
Genetic Evidence ◽  
Coi Gene ◽  
Correct Identification ◽  
Cryptic Diversity ◽  
Separate Species ◽  
Integrative View ◽  
First Time

The small synanthropic and peregrine earthworm Microscolex phosphoreus (Dugès, 1837) is reported for the first time from Siberia. Morphological and DNA barcode (COI) analyses of this and widely separate samples worldwide demonstrate that, as currently identified, M. phosphoreus is a heterogeneous taxon, with divergent lineages occurring often in the same locality and hardly providing geographically structured genetic signals. The combined morphological and genetic evidence suggests that at least four of the found clades should be reclassified as separate species, both morphologically and genetically distinct from each other. However, as the specimen number was limited and only the COI gene was studied for the genetic work, we hesitate in formally describing new species. There would also be the problem of assigning the available names to specific lineages. Our findings encourage careful external and anatomical examination and using reliable characters such as the interchaetal distances and spermathecal morphology for correct identification and for deeper evaluation of cryptic diversity in this interesting bioluminescent worm. 

scholarly journals Discovery of Eubasilissa signata Wiggins, 1998 (Trichoptera, Phryganeidae) from Taiwan

Zoosymposia ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 103-107
Author(s):  
RYOICHI B. KURANISHI ◽  
LI-PENG HSU
Keyword(s):  
Dna Barcode ◽  
Species Group ◽  
Coi Gene ◽  
Single Female ◽  
Separate Species ◽  
Other Information ◽  
Group A ◽  
Morphological Differences ◽  
Male Morphology ◽  
First Time

The phryganeid caddisfly Eubasilissa signata Wiggins, 1998 was originally described based on a single female specimen from Korea, and no other information on the species was available. Here we record an additional five male and five female specimens of E. signata from Taiwan and describe the male morphology for the first time. Based on morphological differences with other Eubasilissa species, E. signata should be regarded as a separate species and also not a member of the “tibetana” species group. A 658-bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene of the new specimens of E. signata is provided as a DNA barcode.

DNA barcoding unravels three new species and a subspecies of Olepa Watson, 1980 (Lepidoptera, Erebidae, Arctiinae) from India, with morphotypes

2020 ◽  
Vol 19 (2) ◽  
pp. 44-60
Author(s):  
APARNA SURESHCHANDRA KALAWATE ◽  
K. P. DINESH ◽  
A. SHABNAM
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Dna Barcode ◽  
Coi Gene ◽  
Male And Female ◽  
Morphological Variations ◽  
Three New Species ◽  
First Time ◽  
Respective Species

The genus Olepa is distributed in Palearctic and Oriental regions with more species in India and Sri Lanka. In the recent studies, morphological variations within the group were well established, with couple of first set of mt COI DNA barcodes for at least three species. In the present account, three new species and a new subspecies are described from the northern Western Ghats region of Maharashtra based on mt COI DNA barcode studies. Due to high morphological divergence and complete genetic homogeneity on the mt COI DNA, four morphotypes under two species are reported. Morphological and genital characters of male and female are provided along with their respective species morphotypes for the first time under this genus from India. The genitalia and the habitus of male and female are illustrated. Preliminary phylogenetic tree based on the mt COI DNA sequences available in the GenBank for the genus with the sequences for the new species also provided and discussed. Key words: new taxa, morphotype, Maharashtra, DNA barcoding, mt COI gene

scholarly journals Neoprotoparmelia gen. nov. and Maronina (Lecanorales, Protoparmelioideae): species description and generic delimitation using DNA barcodes and phenotypical characters

MycoKeys ◽  
2018 ◽  
Vol 44 ◽  
pp. 19-50 ◽  
Author(s):  
Garima Singh ◽  
André Aptroot ◽  
Víctor J. Rico ◽  
Jürgen Otte ◽  
Pradeep K. Divakar ◽  
...  
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Genetic Distances ◽  
Dna Barcodes ◽  
Separate Species ◽  
Generic Delimitation ◽  
Phylogenetic Studies ◽  
High Level ◽  
First Time ◽  
Subtropical Species

Multilocus phylogenetic studies revealed a high level of cryptic diversity within the lichen-forming fungal genus Maronina (Protoparmelioideae, Parmeliaceae). Coalescent-based species delimitation suggested that most of the cryptic molecular lineages warranted recognition as separate species. Here we study the morphology and chemistry of these taxa and formally describe eight new species based on phenotypical and molecular characters. Further, we evaluate the use of ITS rDNA as a DNA barcode for identifying species in this genus. For the first time, we obtained an ITS sequence of Maroninaaustraliensis, the type species of the genus and showed that it is phylogenetically not closely related to species currently placed in Maronina or Protoparmelia. We assembled a dataset of 66 ITS sequences to assess the interspecies genetic distances amongst the twelve Maronina species using ITS as DNA barcode. We found that Maronina and Protoparmelia form a supported monophyletic group whereas M.australiensis is sister to both. We therefore propose a new genus Neoprotoparmelia to accommodate the tropical-subtropical species within Protoparmelioideae, with Neoprotoparmeliacorallifera as the type, N.amerisidiata, N.australisidiata, N.brasilisidiata, N.capensis, N.crassa, N.pauli, N.plurisporibadia and N.siamisidiata as new species and N.capitata, N.isidiata, N.multifera, N.orientalis and N.pulchra as new proposed combinations. We provide a key to Neoprotoparmelia and confirm the use of ITS for accurately identifying species in this group.

scholarly journals DNA barcode library for European Gelechiidae (Lepidoptera) suggests greatly underestimated species diversity

ZooKeys ◽  
2020 ◽  
Vol 921 ◽  
pp. 141-157 ◽  
Author(s):  
Peter Huemer ◽  
Ole Karsholt ◽  
Leif Aarvik ◽  
Kai Berggren ◽  
Oleksiy Bidzilya ◽  
...  
Keyword(s):  
Species Diversity ◽  
Dna Barcode ◽  
Species Level ◽  
Coi Gene ◽  
Species Discrimination ◽  
Nominal Species ◽  
Nearest Neighbour ◽  
Cryptic Diversity ◽  
Dna Barcodes ◽  
First Time

For the first time, a nearly complete barcode library for European Gelechiidae is provided. DNA barcode sequences (COI gene – cytochrome c oxidase 1) from 751 out of 865 nominal species, belonging to 105 genera, were successfully recovered. A total of 741 species represented by specimens with sequences ≥ 500bp and an additional ten species represented by specimens with shorter sequences were used to produce 53 NJ trees. Intraspecific barcode divergence averaged only 0.54% whereas distance to the Nearest-Neighbour species averaged 5.58%. Of these, 710 species possessed unique DNA barcodes, but 31 species could not be reliably discriminated because of barcode sharing or partial barcode overlap. Species discrimination based on the Barcode Index System (BIN) was successful for 668 out of 723 species which clustered from minimum one to maximum 22 unique BINs. Fifty-five species shared a BIN with up to four species and identification from DNA barcode data is uncertain. Finally, 65 clusters with a unique BIN remained unidentified to species level. These putative taxa, as well as 114 nominal species with more than one BIN, suggest the presence of considerable cryptic diversity, cases which should be examined in future revisionary studies.

A new species of the genus Parapentacentrus Shiraki, 1930 from Yunnan, China (Orthoptera: Gryllidae: Itarinae)

Zootaxa ◽  
2021 ◽  
Vol 5032 (1) ◽  
pp. 143-146
Author(s):  
YI-JIAO LIU ◽  
ZHU-QING HE
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Dna Barcode ◽  
Coi Gene ◽  
East China ◽  
Anal Plate ◽  
Type Specimens ◽  
Normal University ◽  
One New Species ◽  
A New Species

The genus Parapentacentrus Shiraki, 1930 includes two species with long wings. In this paper, we report one new species, P. brevipennis He sp. nov., from Jinping, Yunnan, China. The new species have short forewings and hindwings, and have differences in the shape of supra-anal plate and male genitalia. DNA Barcode (COI gene) of this new species are provided. The type specimens are deposited in Museum of Biology, East China Normal University (ECNU).  

scholarly journals Ypsolopha rhinolophi sp. nov. (Lepidoptera: Ypsolophidae), a new species from Portugal and France unveiled by bats

Zootaxa ◽  
2019 ◽  
Vol 4609 (3) ◽  
pp. 565 ◽  
Author(s):  
MARTIN CORLEY ◽  
SÓNIA FERREIRA ◽  
VANESSA A. MATA
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Dna Barcode ◽  
Female Genitalia ◽  
Quercus Pyrenaica ◽  
Male And Female ◽  
Northern Portugal ◽  
First Time ◽  
A New Species ◽  
Horseshoe Bats

A new species Ypsolopha rhinolophi Corley is described from northern Portugal and south-east France. It resembles Y. alpella (Denis & Schiffermüller, 1775) and Y. lucella (Fabricius, 1775) but shows clear differences from both species in DNA barcode and in male and female genitalia. Male genitalia of Y. lucella are illustrated for the first time. The new species has been collected at light, reared from larvae on Quercus pyrenaica Willd. and recognised from DNA barcode fragments obtained from droppings of horseshoe bats. 

New species of Endoclita (Lepidoptera: Hepialidae) and revived species status of E. kosemponis from Taiwan

Zootaxa ◽  
2019 ◽  
Vol 4551 (4) ◽  
pp. 432
Author(s):  
ULF BUCHSBAUM ◽  
JOHN R. GREHAN
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
Female Genitalia ◽  
Coi Gene ◽  
Species Status ◽  
External Appearance ◽  
Male And Female ◽  
Preliminary Comparison ◽  
Future Evaluation ◽  
First Time

Two species of Endoclita C. &. R. Felder, 1874 from Taiwan are evaluated for their taxonomic status: Endoclita meifenga Buchsbaum & Grehan sp. n. is described as new for a unique male from the central mountains of Taiwan, and E. kosemponis (Strand, 1916), stat. rev. is elevated to species status. We show that the forewing markings and genitalic characteristics of E. meifenga sp. n. distinguish this species from all other Endoclita. The external appearance of E. kosemponis is similar to E. sinensis (Moore, 1877) but the two species show distinct differences in the male and female genitalia. Preliminary comparison of E. sinensis from Taiwan and Japan suggests they represent more than one species. A sample of the COI gene was sequenced for E. meifenga sp. n. and the primary types of E. meifenga sp. n. and E. kosemponis sp. n. are illustrated here for the first time. The taxonomic status of the non endemic Taiwanese E. davidi (Poujade, 1886) and E. sinensis needs future evaluation. 

Two new feather mites (Acari: Analgoidea) isolated from the grey-headed woodpecker, Picus canus (Piciformes: Picidae) in Korea

2019 ◽  
Vol 24 (11) ◽  
pp. 2167-2183
Author(s):  
Yeong-deok Han ◽  
Sergey V. Mironov ◽  
Gi-sik Min
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Feather Mites ◽  
Oxidase Subunit ◽  
Two New Species ◽  
First Time

Two new species of feather mites from the superfamily Analgoidea are described from the grey-headed woodpecker, Picus canus, in Korea: Neopteronyssus koreanus sp. nov. (Pteronyssidae) and Proterothrix picinus sp. nov. (Proctophyllodidae: Pterodectinae). Feather mites of the genera Neopteronyssus Mironov, 2002 and Proterothrix Gaud, 1968 are described for the first time in Korea. Morphological descriptions of both new species are complemented with partial sequences of their mitochondrial cytochrome c oxidase subunit I (COI) gene as DNA barcodes.

COI variation in Scandinavian marine species of Tubificoides (Annelida: Clitellata: Tubificidae)

2007 ◽  
Vol 87 (5) ◽  
pp. 1121-1126 ◽  
Author(s):  
Christer Erséus ◽  
Sebastian Kvist
Keyword(s):  
Random Distribution ◽  
Dna Barcode ◽  
Marine Species ◽  
Interspecific Variation ◽  
Coi Gene ◽  
Swedish West Coast ◽  
North West ◽  
The North ◽  
Southern Scandinavia ◽  
First Time

Intra- and interspecific variation in a 658 bp long part of the cytochrome c oxidase subunit 1 (COI) gene of the mitochondrial genome, i.e. a suggested ‘DNA barcode’, was assessed in four north-west European species of the marine tubificid genus Tubificoides: T. benedii, T. amplivasatus, T. heterochaetus and T. kozloffi. Within species mean genetic distance was from 0.10% (T. amplivasatus) to 0.14% (T. benedii), between species from 19.3% to 22.9%. For T. benedii and T. amplivasatus, material collected in two separate areas, The Sound between Denmark and Sweden, and the Koster area about 330 km to the north along the Swedish west coast, showed a geographically random distribution of COI haplotypes, suggesting that each of these two species forms a continuous population in southern Scandinavia. We conclude that the COI gene is suitable as a barcode marker for the secure identification of these species, at least within the area investigated. Tubificoides heterochaetus is reported for the first time from Denmark.

Review of the genus Argyresthia Hübner, [1825] (Lepidoptera: Yponomeutoidea: Argyresthiidae) from China, with descriptions of forty-three new species

Zootaxa ◽  
2017 ◽  
Vol 4292 (1) ◽  
pp. 1 ◽  
Author(s):  
TENGTENG LIU ◽  
SHUXIA WANG ◽  
HOUHUN LI
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Female Genitalia ◽  
Biological Information ◽  
Species Number ◽  
Male And Female ◽  
Chlorella Sp ◽  
Three New Species ◽  
First Time ◽  
Total Species

The Chinese fauna of Argyresthia Hübner, [1825] included by 14 described species prior to this study. In the present paper we describe 43 new species and record seven species new to China. We also recognized six known Chinese species based on the available specimens; eight species previously reported for the fauna, but without available specimens for examination, are systematically placed in their relevant positions. The total species number of Argyresthia in China is brought to 64. The new species include: A. (Blastotere) aureola, sp. nov., A. (B.) sporadolepis, sp. nov., A. (B.) cineretra, sp. nov., A. (B.) affinicineretra, sp. nov., A. (B.) albaureola, sp. nov., A. (B.) densa, sp. nov., A. (B.) punctireticulata, sp. nov., A. (B.) dolichocoremata, sp. nov., A. (B.) longipenella, sp. nov., A. (Argyresthia) biloba, sp. nov., A. (A.) minutilepidota, sp. nov., A. (A.) flavifusca, sp. nov., A. (A.) trigonophylla, sp. nov., A. (A.) chiotorna, sp. nov., A. (A.) hirsuta, sp. nov., A. (A.) papillata, sp. nov., A. (A.) acuminata, sp. nov., A. (A.) curvativa, sp. nov., A. (A.) atomata, sp. nov., A. (A.) orthocera, sp. nov., A. (A.) cardiopetala, sp. nov., A. (A.) mala, sp. nov., A. (A.) longa, sp. nov., A. (A.) decurtata, sp. nov., A. (A.) minutisocia, sp. nov., A. (A.) scalprata, sp. nov., A. (A.) grammosacca, sp. nov., A. (A.) campylotropa, sp. nov., A. (A.) punctuata, sp. nov., A. (A.) chlorella, sp. nov., A. (A.) triangulata, sp. nov., A. (A.) subzonata, sp. nov., A. (A.) basistriata, sp. nov., A. (A.) longalbella, sp. nov., A. (A.) umbrina, sp. nov., A. (A.) umbrinistrigata, sp. nov., A. (A.) surrecta, sp. nov., A. (A.) lanosa, sp. nov., A. (A.) lata, sp. nov., A. (A.) cuprea, sp. nov., A. (A.) aurilata, sp. nov., A. (A.) convexa, sp. nov., and A. (A.) ellipsoidea, sp. nov.        The newly record species include: A. (A.) pygmaeella ([Denis & Schiffermüller], 1775), A. (A.) retinella Zeller, 1839, A. (A.) angusta Moriuti, 1969, A. (A.) alpha Friese & Moriuti, 1968, A. (A.) beta Friese & Moriuti, 1968, A. (A.) kaoyaiensis Moriuti, 1982, and A. (A.) dislocata Meyrick, 1914. The male of A. (A.) trochaula Meyrick, 1938, and the male and female genitalia of A. (A.) aphoristis Meyrick, 1938 and A. (A.) dislocata are described for the first time. The misidentified species A. (A.) assimilis Moriuti, 1977 in China is described as A. (A.) mala, sp. nov. Argyresthia cryptomeriae Yang, nomen non rite publicatum, is validated as Argyresthia anthocephala Meyrick, 1936. Keys to the subgenera and to the Chinese species with available specimens are provided.        Photos of adults and male and female genitalia are provided for all the examined species except two from Taiwan. Field photographs and biological information are provided where available. Five DNA barcode sequences of the two new species, A. (A.) surrecta, sp. nov. and A. (A.) lanosa, sp. nov., are provided for association of the sexes. 

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