Neoprotoparmelia gen. nov. and Maronina (Lecanorales, Protoparmelioideae): species description and generic delimitation using DNA barcodes and phenotypical characters

Multilocus phylogenetic studies revealed a high level of cryptic diversity within the lichen-forming fungal genus Maronina (Protoparmelioideae, Parmeliaceae). Coalescent-based species delimitation suggested that most of the cryptic molecular lineages warranted recognition as separate species. Here we study the morphology and chemistry of these taxa and formally describe eight new species based on phenotypical and molecular characters. Further, we evaluate the use of ITS rDNA as a DNA barcode for identifying species in this genus. For the first time, we obtained an ITS sequence of Maroninaaustraliensis, the type species of the genus and showed that it is phylogenetically not closely related to species currently placed in Maronina or Protoparmelia. We assembled a dataset of 66 ITS sequences to assess the interspecies genetic distances amongst the twelve Maronina species using ITS as DNA barcode. We found that Maronina and Protoparmelia form a supported monophyletic group whereas M.australiensis is sister to both. We therefore propose a new genus Neoprotoparmelia to accommodate the tropical-subtropical species within Protoparmelioideae, with Neoprotoparmeliacorallifera as the type, N.amerisidiata, N.australisidiata, N.brasilisidiata, N.capensis, N.crassa, N.pauli, N.plurisporibadia and N.siamisidiata as new species and N.capitata, N.isidiata, N.multifera, N.orientalis and N.pulchra as new proposed combinations. We provide a key to Neoprotoparmelia and confirm the use of ITS for accurately identifying species in this group.

Download Full-text

Description of new apterous winter species of Leuctra (Plecoptera: Leuctridae) based morphology and DNA barcoding and further records to stonefly fauna of the Caucasus, Georgia

Zootaxa ◽

10.11646/zootaxa.4585.3.9 ◽

2019 ◽

Vol 4585 (3) ◽

pp. 547

Author(s):

VALENTINA A. TESLENKO ◽

DMITRY M. PALATOV ◽

ALEXANDER A. SEMENCHENKO

Keyword(s):

New Species ◽

Dna Barcoding ◽

Interspecific Variation ◽

Genetic Distances ◽

Dna Barcodes ◽

The Caucasus ◽

Diagnostic Characters ◽

Males And Females ◽

Lesser Caucasus ◽

First Time

Leuctra adjariae sp. n. and Leuctra georgiae sp. n. (Plecoptera: Leuctridae) are described as two new apterous stonefly species from the Meskheti Range (Lesser Caucasus) in southwestern Georgia. Descriptions and illustrations are provided for both sexes and diagnostic characters are discussed. Males and females of the two species are associated by DNA barcodes. Comparisons with corresponding regions of COI between L. adjariae sp. n. and L. georgiae sp. n. produced K2P genetic distances of 8.38%, values well associated with interspecific variation. The well-supported monophyly as well as results of an ABGD analysis confirms the validity of both new species. Capnioneura gouanerae Vinçon & Sivec, 2011, previously described and known only from Turkey, is reported for the first time for the Caucasus.

Download Full-text

Green light to an integrative view of Microscolex phosphoreus (Dugès, 1837) (Annelida: Clitellata: Acanthodrilidae)

Zootaxa ◽

10.11646/zootaxa.4496.1.13 ◽

2018 ◽

Vol 4496 (1) ◽

pp. 175 ◽

Cited By ~ 1

Author(s):

EMILIA ROTA ◽

SVANTE MARTINSSON ◽

CHRISTER ERSÉUS ◽

VALENTIN N. PETUSHKOV ◽

NATALJA S. RODIONOVA ◽

...

Keyword(s):

New Species ◽

Dna Barcode ◽

Green Light ◽

Genetic Evidence ◽

Coi Gene ◽

Correct Identification ◽

Cryptic Diversity ◽

Separate Species ◽

Integrative View ◽

First Time

The small synanthropic and peregrine earthworm Microscolex phosphoreus (Dugès, 1837) is reported for the first time from Siberia. Morphological and DNA barcode (COI) analyses of this and widely separate samples worldwide demonstrate that, as currently identified, M. phosphoreus is a heterogeneous taxon, with divergent lineages occurring often in the same locality and hardly providing geographically structured genetic signals. The combined morphological and genetic evidence suggests that at least four of the found clades should be reclassified as separate species, both morphologically and genetically distinct from each other. However, as the specimen number was limited and only the COI gene was studied for the genetic work, we hesitate in formally describing new species. There would also be the problem of assigning the available names to specific lineages. Our findings encourage careful external and anatomical examination and using reliable characters such as the interchaetal distances and spermathecal morphology for correct identification and for deeper evaluation of cryptic diversity in this interesting bioluminescent worm.

Download Full-text

Two new species of Ecpatia Turner, 1902, and the first records of Ecpatia sciachroa Hampson, 1926 and Ecpatia obscura Holloway, 2009 from Thailand (Lepidoptera: Noctuidae, Noctuinae)

Zootaxa ◽

10.11646/zootaxa.4609.3.11 ◽

2019 ◽

Vol 4609 (3) ◽

pp. 574 ◽

Cited By ~ 1

Author(s):

MARKKU J. PELLINEN ◽

MARKO MUTANEN

Keyword(s):

New Species ◽

Related Species ◽

Morphological Characters ◽

Genetic Distances ◽

Valid Species ◽

Dna Barcodes ◽

Two New Species ◽

Noctuid Species ◽

First Time ◽

Lepidoptera Noctuidae

Two new noctuid species, Ecpatia grisescens sp. n., E. spiculivalva sp. n. are described from Thailand based on a combination of morphological characters and DNA barcodes. Morphological structures and genetic distances are compared to those of related species. Ecpatia sciachroa Hampson, 1926 and Ecpatia obscura Holloway, 2009 are reporded from Thailand for the first time, and a checklist of 17 valid species of Ecpatia is provided.

Download Full-text

Taxonomic study on fourteen symphytognathid species from Asia (Araneae, Symphytognathidae)

ZooKeys ◽

10.3897/zookeys.1072.67935 ◽

2021 ◽

Vol 1072 ◽

pp. 1-47

Author(s):

Ya Li ◽

Shuqiang Li ◽

Yucheng Lin

Keyword(s):

New Species ◽

Genetic Distances ◽

Taxonomic Study ◽

New Combinations ◽

New Genera ◽

Dna Barcodes ◽

Distribution Map ◽

Type Specimens ◽

First Time ◽

Time Type

Fourteen symphytognathid species belonging to three genera are examined, including the descriptions of eight new species and two new genera from China, Vietnam, Thailand and Myanmar: Patu Marples, 1951: P. catba S. Li & Lin, sp. nov. (♂, Vietnam), P. dakou S. Li & Lin, sp. nov. (♂♀, China), P. damtao S. Li & Lin, sp. nov. (♂, Vietnam), P. jiangzhou S. Li & Lin, sp. nov. (♀, China), P. jidanweishi Miller, Griswold & Yin, 2009 (♂♀, China), P. nagarat S. Li & Lin, sp. nov. (♂♀, Thailand), P. nigeri Lin & S. Li, 2009 (♀, China), P. putao S. Li & Lin, sp. nov. (♀, Myanmar), P. qiqi Miller, Griswold & Yin, 2009 (♀, China) and P. xiaoxiao Miller, Griswold & Yin, 2009 (♂♀, China); Kirinua S. Li & Lin, gen. nov.: K. maguai S. Li & Lin, sp. nov. (♂♀, China) and K. yangshuo S. Li & Lin, sp. nov. (♂♀, China); Swilda S. Li & Lin, gen. nov.: S. longtou (Miller, Griswold & Yin, 2009), comb. nov. (♂♀, China) is transferred from Crassignatha Wunderlich, 1995 and S. spinathoraxi (Lin & S. Li, 2009), comb. nov. (♂♀, China) is transferred from Patu. Diagnoses, descriptions and illustrations are provided for new taxa, as well as a distribution map. The males of P. xiaoxiao and S. longtou are described for the first time. Type specimens of P. jidanweishi, P. nigeri, P. qiqi, P. xiaoxiao, S. longtou and S. spinathoraxi are re-examined and photographed. All Asian Patu species are revised and two species, P. kishidai Shinkai, 2009 and P. bispina Lin, Pham & S. Li, 2009, are transferred to Crassignatha and proposed as new combinations: Crassignatha kishidaicomb. nov. and C. bispinacomb. nov. In addition, DNA barcodes and genetic distances of ten species treated in this paper were obtained to confirm identification.

Download Full-text

The caridean shrimps of the genus Lysmata Risso, 1816 (Decapoda: Lysmatidae) from Madagascar collected during the Atimo-Vatae expedition: a new species and two new records

European Journal of Taxonomy ◽

10.5852/ejt.2021.774.1535 ◽

2021 ◽

Vol 774 ◽

pp. 155-177

Author(s):

Hossein Ashrafi ◽

J. Antonio Baeza ◽

Zdeněk Ďuriš

Keyword(s):

New Species ◽

New Records ◽

Original Description ◽

Western Atlantic ◽

West Pacific ◽

Phylogenetic Studies ◽

The Caribbean ◽

De Man ◽

First Time ◽

A New Species

The present study focuses on shrimps belonging to the genus Lysmata Risso, 1816, collected from Madagascar during the Atimo Vatae expedition carried out in 2010. Lysmata malagasy sp. nov. is a new species belonging to the clade named “long accessory ramous” or “cosmopolitan” in previous phylogenetic studies. The new species can be distinguished from the only two other representatives of this group in the Indo-west Pacific, L. ternatensis De Man, 1902, and L. trisetacea (Heller, 1861), by the accessory ramus of the lateral antennular flagellum consisting of four elongated articles. Lysmata lipkei Okuno & Fiedler, 2010 is reported here from Madagascar with a remarkable extension of its known range after its original description from Japan. This species has also been reported from Singapore and, as alien species, from Brazil. Lastly, L. kuekenthali De Man, 1902 known from numerous localities in the Indo-West Pacific biogeographic area, is reported for the first time from Madagascar. Results of the present morphological and molecular analyses suggest that L. hochi Baeza & Anker, 2008 from the Caribbean Sea is a synonym of the Indo-West Pacific L. kuekenthali, and thus the latter species is alien in the western Atlantic.

Download Full-text

DNA barcodes and morphology of P. krempfi, P. mekongensis, and P. elongatus

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/14/1/9289 ◽

2016 ◽

Vol 14 (1) ◽

pp. 29-37 ◽

Cited By ~ 1

Author(s):

Dương Thúy Yên ◽

Nguyễn Kiệt ◽

Bùi Sơn Nên ◽

Nguyễn Văn Thường ◽

Nguyễn Bạch Loan ◽

...

Keyword(s):

Dna Barcode ◽

Morphological Characteristics ◽

Mekong Delta ◽

Genetic Distances ◽

Dna Barcodes ◽

Gill Rakers ◽

Coi Sequences ◽

Relationship Of ◽

Metric Traits ◽

I Gene

Three Pangasius species including P. krempfi, P. elongatus and P. mekongensis, are economically important. They can be mis-identified due to similar external appreance at small sizes. This study aimed to distinguish these species based on their differences in DNA barcode, COI (cytochrome c oxidase subunit I) gene, and morphological characteristics. Fish with various sizes (>90 samples/species) were sampled at the lower Mekong delta region. Kimura-2 parameter genetic distances based on COI sequences of three species (15 samples, in which, 4 unique sequences were assigned Genbank accession numbers from KT289877 to KT289880) are relatively high, ranging 9.33 – 12.10 %. Morphological measurements show that coutanble traits including numbers of fin rays and the first gill rakers vary in similar ranges but ratios of metric traits are significantly different among three species (P<0.01). Principle component analysis using metric traits sets three species apart. P. elongatus is characterized by elongated body, long caudal preduncle, large eyes, and retangle palatine tooth plates. P. krempfi differs from P. mekongesis in characteristics on their head. The number of sections, shape and length of barbel are different among three species. Phylogenetic relationship of three species based on morphology and COI sequences indicate that P. krempfi is closer to P. mekongenis rather than P. elongatus, and that the distance between P. mekongenis and P. elongatus is the largest.

Download Full-text

A new species of Litarachna Walter, 1925 (Acari: Hydrachnidia: Pontarachnidae) from Corozal Bay (Belize), described based upon morphology and DNA barcodes

Acarologia ◽

10.24349/r7no-ludg ◽

2021 ◽

Vol 61 (3) ◽

pp. 602-613

Author(s):

Lucia Montes-Ortiz ◽

Tom Goldschmidt ◽

Lourdes Vásquez-Yeomans ◽

Manuel Elías-Gutiérrez

Keyword(s):

Dna Barcode ◽

Morphological Description ◽

Estuarine System ◽

Dna Barcodes ◽

Sem Images ◽

The Family ◽

Coi Sequences ◽

First Time ◽

A New Species ◽

Electronic Microscope

A new planktonic species of the marine water mites of the family Pontarachnidae Koenike, 1910 is described from Corozal Bay, an estuarine system in Belize. The morphological description includes Scanning Electronic Microscope (SEM) images and is augmented by an analysis of DNA cytochrome c oxidase I (COI) sequences, the DNA barcode, used for the first time for a species description in this group.

Download Full-text

Phylogeny and re-definition of the genus Melanophora (Diptera: Rhinophoridae), with description of a new species from Sardinia

Zootaxa ◽

10.11646/zootaxa.2318.1.23 ◽

2009 ◽

Vol 2318 (1) ◽

pp. 552-565 ◽

Cited By ~ 5

Author(s):

PIERFILIPPO CERRETTI ◽

THOMAS PAPE

Keyword(s):

New Species ◽

Type Species ◽

Cladistic Analysis ◽

The Other ◽

New Combinations ◽

Generic Delimitation ◽

Definition Of ◽

First Time ◽

A New Species

A cladistic analysis of the genus Melanophora Meigen, 1803 (type-species: Musca grossificationis Linnaeus, 1758 [= Musca roralis Linnaeus, 1758]) is presented and the generic delimitation is critically redefined. The nominal genus-group taxon Bequaertiana Curran, 1929 (type-species: Bequaertiana argyriventris Curran, 1929) is synonymised with Melanophora Meigen syn. nov. The following new combinations are proposed: Melanophora argyriventris (Curran, 1929) comb. nov. and Melanophora basilewskyi (Peris, 1957) comb. nov. Melanophora chia sp. nov. from SW Sardinia is described, illustrated and compared with the other known species of the genus. The male of Melanophora asetosa Kugler, 1978 is described for the first time. Melanophora basilewskyi (Peris, 1957) is recorded from Kenya for the first time.

Download Full-text

Taxonomy of Micronesian monitors (Reptilia: Squamata: Varanus ): endemic status of new species argues for caution in pursuing eradication plans

Royal Society Open Science ◽

10.1098/rsos.200092 ◽

2020 ◽

Vol 7 (5) ◽

pp. 200092

Author(s):

Valter Weijola ◽

Varpu Vahtera ◽

André Koch ◽

Andreas Schmitz ◽

Fred Kraus

Keyword(s):

New Species ◽

The Other ◽

Disjunct Distribution ◽

Morphological Evidence ◽

Mariana Islands ◽

Separate Species ◽

Natural Origin ◽

Phylogenetic Studies ◽

Human Introduction ◽

Monitor Lizards

In the light of recent phylogenetic studies, we re-assess the taxonomy and biogeography of the Varanus populations distributed in the Micronesian islands of Palau, the Western Carolines and the Marianas. Whether these populations are of natural origin or human introductions has long been contentious, but no study has fully resolved that question. Here, we present molecular and morphological evidence that monitor lizards of the Varanus indicus Group reached both Palau and the Mariana Islands sometime in the late Pleistocene and subsequently differentiated into two separate species endemic to each geographical region. One species is confined to the Mariana Islands, and for these populations, we revalidate the name V. tsukamotoi Kishida, 1929. The other species has a disjunct distribution in Palau, the Western Carolines and Sarigan Island in the Northern Marianas and is herein described as V. bennetti sp. nov. Both species are most closely allied to each other, V. lirungensis and V. rainerguentheri , suggesting that colonization of Micronesia took place from the Moluccas. We discuss the biogeographic distributions of both species in the light of the likely colonization mechanism and previous arguments for human introduction, and we argue that bounties for Palauan populations are ill-advised and plans for eradication of some other populations must first demonstrate that they are, in fact, introduced and not native.

Download Full-text

Ypsolopha rhinolophi sp. nov. (Lepidoptera: Ypsolophidae), a new species from Portugal and France unveiled by bats

Zootaxa ◽

10.11646/zootaxa.4609.3.10 ◽

2019 ◽

Vol 4609 (3) ◽

pp. 565 ◽

Cited By ~ 6

Author(s):

MARTIN CORLEY ◽

SÓNIA FERREIRA ◽

VANESSA A. MATA

Keyword(s):

New Species ◽

Male Genitalia ◽

Dna Barcode ◽

Female Genitalia ◽

Quercus Pyrenaica ◽

Male And Female ◽

Northern Portugal ◽

First Time ◽

A New Species ◽

Horseshoe Bats

A new species Ypsolopha rhinolophi Corley is described from northern Portugal and south-east France. It resembles Y. alpella (Denis & Schiffermüller, 1775) and Y. lucella (Fabricius, 1775) but shows clear differences from both species in DNA barcode and in male and female genitalia. Male genitalia of Y. lucella are illustrated for the first time. The new species has been collected at light, reared from larvae on Quercus pyrenaica Willd. and recognised from DNA barcode fragments obtained from droppings of horseshoe bats.

Download Full-text