scholarly journals Structure of the Midgut of the Queen Bee <i>Apis mellifera</i> <I>L.</I> Naturally Infected with <i>Sacbrood virus</i> (SBV)

2021 ◽  
Vol 9 (3) ◽  
pp. 73
Author(s):  
Iurii Kovalskyi ◽  
Vasylyna Fedak ◽  
Lidiya Kovalska ◽  
Andrii Druzhbiak ◽  
Yaroslav Vovkun
Keyword(s):  
Apis Mellifera ◽  
Sacbrood Virus

scholarly journals Presence and Prevalence of Viruses in Local and Migratory Honeybees (Apis mellifera) in Massachusetts

2009 ◽  
Vol 75 (24) ◽  
pp. 7862-7865 ◽  
Author(s):  
Anna Welch ◽  
Francis Drummond ◽  
Sunil Tewari ◽  
Anne Averill ◽  
John P. Burand
Keyword(s):  
Apis Mellifera ◽  
Black Queen Cell Virus ◽  
Deformed Wing Virus ◽  
Sacbrood Virus ◽  
Queen Cell ◽  
The Impact

ABSTRACT Migratory and local bees in Massachusetts were analyzed for seven viruses. Three were detected: black queen cell virus (BQCV), deformed wing virus (DWV), and sacbrood virus (SBV). DWV was most common, followed closely by BQCV and then by SBV. BQCV and SBV were present at significantly higher rates in the migratory bees assayed, bringing into question the impact that these bees have on the health of local bee populations.

Dynamics of Apis cerana Sacbrood Virus (AcSBV) Prevalence in Apis cerana (Hymenoptera: Apidae) in Northern Taiwan and Demonstration of its Infection in Apis mellifera (Hymenoptera: Apidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2055-2066 ◽  
Author(s):  
Chong-Yu Ko ◽  
Zong-Lin Chiang ◽  
Ruo-Jyun Liao ◽  
Zih-Ting Chang ◽  
Ju-Chun Chang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Apis Cerana ◽  
Cross Infection ◽  
Prevalence Rates ◽  
Sacbrood Virus ◽  
Northern Taiwan ◽  
Long Term Survey

AbstractSince 2016, Apis cerana sacbrood virus (AcSBV) has been recorded in Taiwan. It is epizootic in Apis cerana (Hymenoptera: Apidae) and causing serious loss of A. cerana. Herein, we performed a long-term survey of AcSBV prevalence in the populations of A. cerana in Northern Taiwan from January 2017 to July 2018. The surveillance of AcSBV prevalence in A. mellifera (Hymenoptera: Apidae) populations was starting and further confirmed by sequencing since April 2017; thus, these data were also included in this survey. In our survey, the average prevalence rates of AcSBV were 72 and 53% in A. cerana and A. mellifera, respectively, in 2017, which decreased to 45 and 27% in 2018. For the spatial analysis of AcSBV in two honey bee populations, Hsinchu showed the highest prevalence, followed by New Taipei, Yilan, Taipei, and Keelung, suggesting that AcSBV might have come from the southern part of Taiwan. Interestingly, the AcSBV prevalence rates from A. cerana and A. mellifera cocultured apiaries gradually synchronized. The result of phylogenetic analysis and comparison of the annual AcSBV prevalence in A. cerana-only, A. mellifera-only, and A. cerana/A. mellifera cocultured sample sites indicate cross-infection between A. cerana and A. mellifera; however, AcSBV may lose the advantage of virulence in A. mellifera. The evidence suggested that the transmission of AcSBV might occur among these two honey bee species in the field. Therefore, A. mellifera may serve as a guard species to monitor AcSBV in A. cerana, but the cross-infection still needs to be surveyed.

Sacbrood virus of the larval honey bee (Apis mellifera linnaeus)

Virology ◽  
1964 ◽  
Vol 23 (3) ◽  
pp. 425-429 ◽  
Author(s):  
L. Bailey ◽  
A.J. Gibbs ◽  
R.D. Woods
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Sacbrood Virus

scholarly journals Sacbrood Virus of the Honeybee (Apis mellifera): Rapid Identification and Phylogenetic Analysis Using Reverse Transcription-PCR

2001 ◽  
Vol 8 (1) ◽  
pp. 93-104 ◽  
Author(s):  
Elvira Grabensteiner ◽  
Wolfgang Ritter ◽  
Michael J. Carter ◽  
Sean Davison ◽  
Hermann Pechhacker ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Mellifera ◽  
Reverse Transcription ◽  
Direct Detection ◽  
Geographic Origin ◽  
Rapid Identification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Sacbrood Virus ◽  
Reverse Transcription Pcr

ABSTRACT Sacbrood virus (SBV) infects larvae of the honeybee (Apis mellifera), resulting in failure to pupate and death. Until now, identification of viruses in honeybee infections has been based on traditional methods such as electron microscopy, immunodiffusion, and enzyme-linked immunosorbent assay. Culture cannot be used because no honeybee cell lines are available. These techniques are low in sensitivity and specificity. However, the complete nucleotide sequence of SBV has recently been determined, and with these data, we now report a reverse transcription-PCR (RT-PCR) test for the direct, rapid, and sensitive detection of these viruses. RT-PCR was used to target five different areas of the SBV genome using infected honeybees and larvae originating from geographically distinct regions. The RT-PCR assay proved to be a rapid, specific, and sensitive diagnostic tool for the direct detection of SBV nucleic acid in samples of infected honeybees and brood regardless of geographic origin. The amplification products were sequenced, and phylogenetic analysis suggested the existence of at least three distinct genotypes of SBV.

scholarly journals The Phylogeny and Pathogenesis of Sacbrood Virus (SBV) Infection in European Honey Bees, Apis mellifera

Viruses ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 61 ◽  
Author(s):  
Jianghong Li ◽  
Tingyun Wang ◽  
Jay Evans ◽  
Robyn Rose ◽  
Yazhou Zhao ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Mellifera ◽  
Cold Stress ◽  
Honey Bees ◽  
Early Spring ◽  
Coding Region ◽  
Genome Sequences ◽  
Sacbrood Virus ◽  
High Prevalence ◽  
Host Affiliation

RNA viruses that contain single-stranded RNA genomes of positive sense make up the largest group of pathogens infecting honey bees. Sacbrood virus (SBV) is one of the most widely distributed honey bee viruses and infects the larvae of honey bees, resulting in failure to pupate and death. Among all of the viruses infecting honey bees, SBV has the greatest number of complete genomes isolated from both European honey bees Apis mellifera and Asian honey bees A. cerana worldwide. To enhance our understanding of the evolution and pathogenicity of SBV, in this study, we present the first report of whole genome sequences of two U.S. strains of SBV. The complete genome sequences of the two U.S. SBV strains were deposited in GenBank under accession numbers: MG545286.1 and MG545287.1. Both SBV strains show the typical genomic features of the Iflaviridae family. The phylogenetic analysis of the single polyprotein coding region of the U.S. strains, and other GenBank SBV submissions revealed that SBV strains split into two distinct lineages, possibly reflecting host affiliation. The phylogenetic analysis based on the 5′UTR revealed a monophyletic clade with the deep parts of the tree occupied by SBV strains from both A. cerane and A. mellifera, and the tips of branches of the tree occupied by SBV strains from A. mellifera. The study of the cold stress on the pathogenesis of the SBV infection showed that cold stress could have profound effects on sacbrood disease severity manifested by increased mortality of infected larvae. This result suggests that the high prevalence of sacbrood disease in early spring may be due to the fluctuating temperatures during the season. This study will contribute to a better understanding of the evolution and pathogenesis of SBV infection in honey bees, and have important epidemiological relevance.

Sign in / Sign up

Export Citation Format

Share Document