Starburst Poly(amidoamine) Dendrimer Grafted Gold Nanoparticles as a Scaffold for Folic Acid-Targeted Plasmid DNA Delivery In Vitro

2019 ◽  
Vol 19 (4) ◽  
pp. 1959-1970 ◽  
Author(s):  
Londiwe Simphiwe Mbatha ◽  
Moganavelli Singh
Keyword(s):  
Gold Nanoparticles ◽  
Folic Acid ◽  
Plasmid Dna ◽  
Dna Delivery

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

scholarly journals Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

2013 ◽  
pp. 1361 ◽  
Author(s):  
Shinji Takeoka ◽  
Sarker ◽  
Aoshima ◽  
Hokama ◽  
Inoue ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery ◽  
Cationic Liposomes ◽  
Low Cytotoxicity

Lipid-Coated Gold Nanoparticles Functionalized by Folic Acid as Gene Vectors for Targeted Gene Delivery in vitro and in vivo

ChemMedChem ◽  
2017 ◽  
Vol 12 (21) ◽  
pp. 1768-1775 ◽  
Author(s):  
Baoji Du ◽  
Xiaoxiao Gu ◽  
Xu Han ◽  
Guanyu Ding ◽  
Yuling Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Folic Acid ◽  
Gene Delivery ◽  
Targeted Gene Delivery ◽  
Gene Vectors

scholarly journals Fabrication Of Gold Nanoparticles In Absence Of Surfactant As In Vitro Carrier Of Plasmid DNA

2019 ◽  
Vol Volume 14 ◽  
pp. 8399-8408 ◽  
Author(s):  
Rania S Abdel-Rashid ◽  
Samia M Omar ◽  
Mohammed S Teiama ◽  
Ahmed Khairy ◽  
Mohamed Magdy ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Plasmid Dna

scholarly journals Use in vitro of Gold Nanoparticles Functionalized with Folic Acid as a Photothermal Agent on Treatment of HeLa Cells

2018 ◽  
Vol 62 (1) ◽  
Author(s):  
Linda Bertel Garay ◽  
Fernando Martínez Ortega ◽  
Stelia Carolina Méndez-Sanchez
Keyword(s):  
Gold Nanoparticles ◽  
Folic Acid ◽  
Cancer Cells ◽  
Hela Cells ◽  
Near Infrared ◽  
Infrared Light ◽  
Molar Ratio ◽  
Functionalized Gold Nanoparticles ◽  
Dose Dependent

<p>Folic acid (FA) is used as a recognition molecule to achieve selective internalization in cancer cells. Here functionalized gold nanoparticles with folic acid (AuNP-FA) are proposed as suitable therapeutic agents for cervix cancer cells by photothermal damage. The functionalized gold nanoparticles with folic acid were synthesized by mixing hydrogen tetrachloroaurate with folic acid in a molar ratio of 0.56/1 under radiation of mercury lamp (λ<sub>max</sub>=254 nm). HeLa cells were incubated with AuNP-FA during 48 h, then were irradiated and the cytotoxicity was analyzed 12 h after irradiation. The AuNP-FA were dose-dependent cytotoxic under irradiation and not cytotoxic in the absence of radiation. The viability of cancer cells treated with functionalized and non-functionalized gold nanoparticles (AuNPs), with and without near infrared light at 808 nm, was measured by MTT assays. This work provides useful guidance toward the synthesis of biocompatible nanomaterials for biological applications.</p>

scholarly journals Investigation of Plasmid DNA Delivery and Cell Viability Dynamics for Optimal Cell Electrotransfection In Vitro

2020 ◽  
Vol 10 (17) ◽  
pp. 6070
Author(s):  
Sonam Chopra ◽  
Paulius Ruzgys ◽  
Martynas Maciulevičius ◽  
Milda Jakutavičiūtė ◽  
Saulius Šatkauskas
Keyword(s):  
Cell Membrane ◽  
Cell Viability ◽  
High Voltage ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Dna Delivery ◽  
Output Parameter ◽  
Gene Electrotransfer ◽  
Electric Pulses

Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in electroporation. Here, we present a combined study in which the dynamics of both evaluation types of transfection efficiency and the cell viability were evaluated in dependence of plasmid concentration as well as at the different number of high voltage (HV) electric pulses. The results of this study reveal a quantitative sigmoidal (R2 > 0.95) dependence of the transfection efficiency and cell viability on the distance between the cell membrane and the nearest plasmid. We propose this distance value as a new, more accurate output parameter that could be used in further optimization studies as a predictor and a measure of electrotransfection efficiency.

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