scholarly journals A Novel, Real-Time, In Vivo Mouse Retinal Imaging System

2015 ◽  
Vol 56 (12) ◽  
pp. 7159 ◽  
Author(s):  
Mark C. Butler ◽  
Jack M. Sullivan
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Retinal Imaging

scholarly journals Performance of a novel protease-activated fluorescent imaging system for intraoperative detection of residual breast cancer during breast conserving surgery

2021 ◽  
Vol 187 (1) ◽  
pp. 145-153
Author(s):  
Conor R. Lanahan ◽  
Bridget N. Kelly ◽  
Michele A. Gadd ◽  
Michelle C. Specht ◽  
Carson L. Brown ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Imaging System ◽  
Ex Vivo ◽  
Menopausal Status ◽  
Fluorescent Imaging ◽  
Cancer Subtypes ◽  
Surgical Workflow ◽  
Tumor Types

Abstract Purpose Safe breast cancer lumpectomies require microscopically clear margins. Real-time margin assessment options are limited, and 20–40% of lumpectomies have positive margins requiring re-excision. The LUM Imaging System previously showed excellent sensitivity and specificity for tumor detection during lumpectomy surgery. We explored its impact on surgical workflow and performance across patient and tumor types. Methods We performed IRB-approved, prospective, non-randomized studies in breast cancer lumpectomy procedures. The LUM Imaging System uses LUM015, a protease-activated fluorescent imaging agent that identifies residual tumor in the surgical cavity walls. Fluorescent cavity images were collected in real-time and analyzed using system software. Results Cavity and specimen images were obtained in 55 patients injected with LUM015 at 0.5 or 1.0 mg/kg and in 5 patients who did not receive LUM015. All tumor types were distinguished from normal tissue, with mean tumor:normal (T:N) signal ratios of 3.81–5.69. T:N ratios were 4.45 in non-dense and 4.00 in dense breasts (p = 0.59) and 3.52 in premenopausal and 4.59 in postmenopausal women (p = 0.19). Histopathology and tumor receptor testing were not affected by LUM015. Falsely positive readings were more likely when tumor was present < 2 mm from the adjacent specimen margin. LUM015 signal was stable in vivo at least 6.5 h post injection, and ex vivo at least 4 h post excision. Conclusions Intraoperative use of the LUM Imaging System detected all breast cancer subtypes with robust performance independent of menopausal status and breast density. There was no significant impact on histopathology or receptor evaluation.

scholarly journals Real-Time In Vivo Bioluminescent Imaging for Evaluating the Efficacy of Antibiotics in a Rat Staphylococcus aureus Endocarditis Model

2005 ◽  
Vol 49 (1) ◽  
pp. 380-387 ◽  
Author(s):  
Yan Q. Xiong ◽  
Julie Willard ◽  
Jagath L. Kadurugamuwa ◽  
Jun Yu ◽  
Kevin P. Francis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Imaging System ◽  
Bioluminescent Imaging ◽  
Vancomycin Therapy ◽  
Treatment Groups ◽  
Highly Sensitive ◽  
Relevant Animal Model ◽  
And Control

ABSTRACT Therapeutic options for invasive Staphylococcus aureus infections have become limited due to rising antimicrobial resistance, making relevant animal model testing of new candidate agents more crucial than ever. In the present studies, a rat model of aortic infective endocarditis (IE) caused by a bioluminescently engineered, biofilm-positive S. aureus strain was used to evaluate real-time antibiotic efficacy directly. This strain was vancomycin and cefazolin susceptible but gentamicin resistant. Bioluminescence was detected and quantified daily in antibiotic-treated and control animals with IE, using a highly sensitive in vivo imaging system (IVIS). Persistent and increasing cardiac bioluminescent signals (BLS) were observed in untreated animals. Three days of vancomycin therapy caused significant reductions in both cardiac BLS (>10-fold versus control) and S. aureus densities in cardiac vegetations (P < 0.005 versus control). However, 3 days after discontinuation of vancomycin therapy, a greater than threefold increase in cardiac BLS was observed, indicating relapsing IE (which was confirmed by quantitative culture). Cefazolin resulted in modest decreases in cardiac BLS and bacterial densities. These microbiologic and cardiac BLS differences during therapy correlated with a longer time-above-MIC for vancomycin (>12 h) than for cefazolin (∼4 h). Gentamicin caused neither a reduction in cardiac S. aureus densities nor a reduction in BLS. There were significant correlations between cardiac BLS and S. aureus densities in vegetations in all treatment groups. These data suggest that bioluminescent imaging provides a substantial advance in the real-time monitoring of the efficacy of therapy of invasive S. aureus infections in live animals.

scholarly journals Statistical analysis of ocular monochromatic aberrations in Chinese population for adaptive optics ophthalmoscope design

2017 ◽  
Vol 10 (01) ◽  
pp. 1650038 ◽  
Author(s):  
Junlei Zhao ◽  
Fei Xiao ◽  
Jian Kang ◽  
Haoxin Zhao ◽  
Yun Dai ◽  
...  
Keyword(s):  
High Resolution ◽  
Adaptive Optics ◽  
Chinese Population ◽  
Imaging System ◽  
Retinal Imaging ◽  
Clinical Environment ◽  
Monochromatic Aberrations ◽  
Imaging System Design ◽  
Healthy Eyes

It is necessary to know the distribution of the Chinese eye’s aberrations in clinical environment to guide high-resolution retinal imaging system design for large Chinese population application. We collected the monochromatic wave aberration of 332 healthy eyes and 344 diseased eyes in Chinese population across a 6.0-mm pupil. The aberration statistics of Chinese eyes including healthy eyes and diseased eyes were analyzed, and some differences of aberrations between the Chinese and European race were concluded. On this basis, the requirement for adaptive optics (AO) correction of the Chinese eye’s monochromatic aberrations was analyzed. The result showed that a stroke of 20[Formula: see text][Formula: see text]m and ability to correct aberrations up to the 8th Zernike order were needed for reflective wavefront correctors to achieve near diffraction-limited imaging in both groups for a reference wavelength of 550[Formula: see text]nm and a pupil diameter of 6.0[Formula: see text]mm. To verify the analysis mentioned above, an AO flood-illumination system was established, and high-resolution retinal imaging in vivo was achieved for Chinese eye including both healthy and diseased eyes.

scholarly journals Real-Time Fluorescence Imaging Using Indocyanine Green to Assess Therapeutic Effects of Near-Infrared Photoimmunotherapy in Tumor Model Mice

2020 ◽  
Vol 19 ◽  
pp. 153601212093496
Author(s):  
Adrian Rosenberg ◽  
Daiki Fujimura ◽  
Ryuhei Okada ◽  
Aki Furusawa ◽  
Fuyuki Inagaki ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Tumor Model ◽  
Therapeutic Effects ◽  
Tumor Perfusion ◽  
Background Ratio

Background: Near-infrared photoimmunotherapy (NIR-PIT) is a cancer therapy that causes an increase in tumor perfusion, a phenomenon termed the super-enhanced permeability and retention effect. Currently, in vivo treatment efficacy of NIR-PIT is observable days after treatment, but monitoring would be improved by more acute detection of intratumor change. Fluorescence imaging may detect increased tumor perfusion immediately after treatment. Methods: In the first experiment, athymic nude mouse models bearing unilateral subcutaneous flank tumors were treated with either NIR-PIT or laser therapy only. In the second experiment, mice bearing bilateral flank tumors were treated with NIR-PIT only on the left-sided tumor. In both groups, immediately after treatment, indocyanine green was injected at different doses intravenously, and mice were monitored with the Shimadzu LIGHTVISION fluorescence imaging system for 1 hour. Results: Tumor-to-background ratio of fluorescence intensity increased over the 60 minutes of monitoring in treated mice but did not vary significantly in control mice. Tumor-to-background ratio was highest in the 1 mg kg−1 and 0.3 mg kg−1 doses. In mice with bilateral tumors, tumor-to-untreated tumor ratio increased similarly. Conclusions: Acute changes in tumor perfusion after NIR-PIT can be detected by real-time fluorescence imaging.

Progress in Two-Dimensional Arrays for Real-Time Volumetric Imaging

1998 ◽  
Vol 20 (1) ◽  
pp. 1-15 ◽  
Author(s):  
E. D. Light ◽  
R. E. Davidsen ◽  
J.O. Fiering ◽  
T. A. Hruschka ◽  
S. W. Smith
Keyword(s):  
Real Time ◽  
Insertion Loss ◽  
Imaging System ◽  
Two Dimensional ◽  
Volumetric Imaging ◽  
Dimensional Array ◽  
Transducer Arrays ◽  
Volumetric Images ◽  
Duke University

The design, fabrication, and evaluation of two dimensional array transducers for real-time volumetric imaging are described. The transducers we have previously described operated at frequencies below 3 MHz and were unwieldy to the operator because of the interconnect schemes used in connecting to the transducer handle. Several new transducers have been developed using new connection technology. A 40 × 40 = 1,600 element, 3.5 MHz array was fabricated with 256 transmit and 256 receive elements. A 60 × 60 = 3,600 element 5.0 MHz array was constructed with 248 transmit and 256 receive elements. An 80 × 80 = 6,400 element, 2.5 MHz array was fabricated with 256 transmit and 208 receive elements. 2-D transducer arrays were also developed for volumetric scanning in an intracardiac catheter, a 10 × 10 = 100 element 5.0 MHz forward-looking array and an 11 × 13 = 143 element 5.0 MHz side-scanning array. The −6 dB fractional bandwidths for the different arrays varied from 50% to 63%, and the 50 Ω insertion loss for all the transducers was about −64 dB. The transducers were used to generate real-time volumetric images in phantoms and in vivo using the Duke University real time volumetric imaging system, which is capable of generating multiple planes at any desired angle and depth within the pyramidal volume.

scholarly journals A Novel Imaging System Permits Real-time in Vivo Tumor Bed Assessment After Resection of Naturally Occurring Sarcomas in Dogs

2013 ◽  
Vol 471 (3) ◽  
pp. 834-842 ◽  
Author(s):  
William C. Eward ◽  
Jeffrey K. Mito ◽  
Cindy A. Eward ◽  
Jessica E. Carter ◽  
Jorge M. Ferrer ◽  
...  
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Tumor Bed ◽  
Naturally Occurring

scholarly journals Real-Time In Vivo Detection and Monitoring of Bacterial Infection Based on NIR-II Imaging

2021 ◽  
Vol 9 ◽  
Author(s):  
Sijia Feng ◽  
Huizhu Li ◽  
Chang Liu ◽  
Mo Chen ◽  
Huaixuan Sheng ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Real Time ◽  
Bacterial Infections ◽  
Near Infrared ◽  
Imaging System ◽  
Bacterial Load ◽  
Dynamic Imaging ◽  
In Vivo Detection

Treatment according to the dynamic changes of bacterial load in vivo is critical for preventing progression of bacterial infections. Here, we present a lead sulfide quantum dots (PbS QDs) based second near-infrared (NIR-II) fluorescence imaging strategy for bacteria detection and real-time in vivo monitoring. Four strains of bacteria were labeled with synthesized PbS QDs which showed high bacteria labeling efficiency in vitro. Then bacteria at different concentrations were injected subcutaneously on the back of male nude mice for in vivo imaging. A series of NIR-II images taken at a predetermined time manner demonstrated changing patterns of photoluminescence (PL) intensity of infected sites, dynamically imaging a changing bacterial load in real-time. A detection limit around 102–104 CFU/ml was also achieved in vivo. Furthermore, analysis of pathology of infected sites were performed, which showed high biocompatibility of PbS QDs. Therefore, under the guidance of our developed NIR-II imaging system, real-time detection and spatiotemporal monitoring of bacterial infection in vivo can be achieved, thus facilitating anti-infection treatment under the guidance of the dynamic imaging of bacterial load in future.

scholarly journals In VivoLabelling of Adenovirus DNA Identifies Chromatin Anchoring and Biphasic Genome Replication

2018 ◽  
Vol 92 (18) ◽  
Author(s):  
Tetsuro Komatsu ◽  
Charlotte Quentin-Froignant ◽  
Irene Carlon-Andres ◽  
Floriane Lagadec ◽  
Fabienne Rayne ◽  
...  
Keyword(s):  
Life Cycle ◽  
Real Time ◽  
Viral Genome ◽  
Imaging System ◽  
Morphological Changes ◽  
Host Cells ◽  
Genome Replication ◽  
Equal Distribution ◽  
Viral Genomes

ABSTRACTAdenoviruses are DNA viruses with a lytic infection cycle. Following the fate of incoming as well as recently replicated genomes during infections is a challenge. In this study, we used the ANCHOR3 technology based on a bacterial partitioning system to establish a versatilein vivoimaging system for adenoviral genomes. The system allows the visualization of both individual incoming and newly replicated genomes in real time in living cells. We demonstrate that incoming adenoviral genomes are attached to condensed cellular chromatin during mitosis, facilitating the equal distribution of viral genomes in daughter cells after cell division. We show that the formation of replication centers occurs in conjunction within vivogenome replication and determine replication rates. Visualization of adenoviral DNA revealed that adenoviruses exhibit two kinetically distinct phases of genome replication. Low-level replication occurred during early replication, while high-level replication was associated with late replication phases. The transition between these phases occurred concomitantly with morphological changes of viral replication compartments and with the appearance of virus-induced postreplication (ViPR) bodies, identified by the nucleolar protein Mybbp1A. Taken together, our real-time genome imaging system revealed hitherto uncharacterized features of adenoviral genomesin vivo. The system is able to identify novel spatiotemporal aspects of the adenovirus life cycle and is potentially transferable to other viral systems with a double-stranded DNA phase.IMPORTANCEViruses must deliver their genomes to host cells to ensure replication and propagation. Characterizing the fate of viral genomes is crucial to understand the viral life cycle and the fate of virus-derived vector tools. Here, we integrated the ANCHOR3 system, anin vivoDNA-tagging technology, into the adenoviral genome for real-time genome detection. ANCHOR3 tagging permitted thein vivovisualization of incoming genomes at the onset of infection and of replicated genomes at late phases of infection. Using this system, we show viral genome attachment to condensed host chromosomes during mitosis, identifying this mechanism as a mode of cell-to-cell transfer. We characterize the spatiotemporal organization of adenovirus replication and identify two kinetically distinct phases of viral genome replication. The ANCHOR3 system is the first technique that allows the continuous visualization of adenoviral genomes during the entire virus life cycle, opening the way for further in-depth study.

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