scholarly journals Immunohistochemical and qPCR Detection of SARS-CoV-2 in the Human Middle Ear Versus the Nasal Cavity: Case Series

2021 ◽  
Author(s):  
Arwa Kurabi ◽  
Kwang Pak ◽  
Adam S. DeConde ◽  
Allen F. Ryan ◽  
Carol H. Yan
Keyword(s):  
Nasal Cavity ◽  
Middle Ear ◽  
Viral Infections ◽  
Case Series ◽  
Middle Ear Mucosa ◽  
Viral Receptor ◽  
Tissue Samples ◽  
Angiotensin Converting Enzyme 2 ◽  
Nasal Tissue ◽  
Human Middle Ear

AbstractViral infections have already been implicated with otitis media and sudden sensorineural hearing loss. However, the pathophysiology of COVID-19 as it relates to otologic disorders is not well-defined. With the spread of SARS-CoV-2, it is important to evaluate its colonization of middle ear mucosa. Middle ear and nasal tissue samples for quantitative RT-PCR and histologic evaluations were obtained from post-mortem COVID-19 patients and non-diseased control patients. Here we present evidence that SARS-CoV-2 colonizes the middle ear epithelium and co-localizes with the primary viral receptor, angiotensin-converting enzyme 2 (ACE2). Both middle ear and nasal epithelial cells show relatively high expression of ACE2, required for SARS-CoV-2 entry. The epithelial cell adhesion molecule (EpCAM) was use as a biomarker of epithelia. Furthermore, we found that the viral load in the middle ear is lower than that present in the nasal cavity.

Are mucin genes yet to be cloned expressed by human middle ear mucosa?

1998 ◽  
Vol 23 (3) ◽  
pp. 265-265 ◽  
Author(s):  
Hutton ◽  
Birchall ◽  
French ◽  
Kubba ◽  
Severn ◽  
...  
Keyword(s):  
Middle Ear ◽  
Middle Ear Mucosa ◽  
Mucin Genes ◽  
Human Middle Ear

scholarly journals Transcanal Endoscopic Antrostomy Hole : Its Utility in Restoring Ventilation Pathway to Epitympanum and Mastoid Antrum During Tympanoplasty

2014 ◽  
Vol 2 (1) ◽  
pp. 9-14
Author(s):  
Sheikh Shawkat Kamal
Keyword(s):  
Middle Ear ◽  
Tertiary Care ◽  
Case Series ◽  
Care Hospital ◽  
Retrospective Case ◽  
Middle Ear Mucosa ◽  
Case Series Study ◽  
Temporal Bones ◽  
Mastoid Antrum

Aim: To evaluate the role of newly created transcanal endoscopic antrostomy hole as a passage in restoring the aeration of epitympanum and mastoid antrum.Materials & Methods: Study design: Retrospective case series study. Study duration: From January 2013 to January 2014. Study place: Private tertiary care hospital. Patients: 27  ears of 23 adult patients (age ranging from 15years to 54years) underwent transcanal endoscopic tympanoplasty for their chronic middle ear diseases in presence of inflamed middle ear mucosa along with radiological shadows of stagnant fluid in their epitympanum and mastoid antrum. Structured three months follow-up was ensured in every case. Intervention: A hole was created at a selected site of posterior meatal wall purely through transcanal endoscopic approach that established direct communication between mastoid antrum and external auditory canal. Thereafter this newly created passage was used for three purposes- assessment of the condition of mastoid antrum, performing the water test for checking epitympanic patency and in few cases placement of temporary tube for postoperative ventilation and drainage of middle ear. Main out come measures: The feasibility, performance and management of transcanal endoscopic antrostomy hole as passage for reestablishing the aeration of epitympanum and mastoid antrum.Results: The chosen site for antrostomy hole was found effective and safe in providing convenient entrance into the mastoid antrum in every case in this study with out facing technical complexity and failure. Postoperative healing of skin over antrostomy hole was found complete in all ears without any inward growth of skin in to mastoid antrum. Available post operative CT scan imaging of temporal bones showed improved aeration in their epitympanum and mastoid antrum.Conclusions: The role of transcanal antrostomy hole has been proved worthy in restoring ventilation pathway to epitympanum and mastoid antrum during tympanoplasty. This antrostomy hole has the potentiality to be considered in future for placement of long term mastoid ventilation tube in order to treat persistent atelectatic middle ear.

scholarly journals Regeneratad Human Middle Ear Mucosa in Tympanoplasty

1979 ◽  
Vol 72 (12) ◽  
pp. 1647-1654
Author(s):  
Eiji Yumoto ◽  
Naoaki Yanagihara
Keyword(s):  
Middle Ear ◽  
Middle Ear Mucosa ◽  
Human Middle Ear

scholarly journals Investigation of the Presence of Biofilms in Chronic Suppurative Otitis Media, Nonsuppurative Otitis Media, and Chronic Otitis Media with Cholesteatoma by Scanning Electron Microscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ercan Kaya ◽  
Ilknur Dag ◽  
Armagan Incesulu ◽  
Melek Kezban Gurbuz ◽  
Mustafa Acar ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Chronic Suppurative Otitis Media ◽  
Middle Ear Mucosa ◽  
Suppurative Otitis Media ◽  
Tissue Samples ◽  
Scanning Electron

Objective. Biofilms have been shown to play a major role in the pathogenesis of otolaryngologic infections. However, very limited studies have been undertaken to demonstrate the presence of biofilms in tissues from patients with chronic otitis media (COM) with or without cholesteatoma. Our objective is to study the presence of biofilms in humans with chronic suppurative and nonsuppurative otitis media and cholesteatoma.Study Design. In all, 102 tissue specimens (middle ear, mastoid tissue, and ossicle samples) were collected during surgery from 34 patients.Methods. The samples were processed for the investigation of biofilms by scanning electron microscopy (SEM).Results. Our research supports the hypothesis in which biofilms are involved in chronic suppurative otitis media, cholesteatoma, and, to a lesser degree, chronic nonsuppurative otitis media. There were higher rates in hypertrophic and granulated tissue samples than in normal mucosa. In addition, the presence of biofilms was significantly higher in the middle ear mucosa compared with the mastoid and ossicle samples.Conclusion. In the clinic, the careful use of topical or systemic antimicrobials is essential, and, during surgery, hypertrophic tissue must be carefully removed from normal tissue.

Localization of Prostaglandin-Forming Cyclooxygenase in Middle Ear and External Canal Tissue

1983 ◽  
Vol 91 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Timothy T.K. Jung ◽  
S.K. Juhn ◽  
Alfred F. Michael
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Middle Ear ◽  
Mononuclear Leukocytes ◽  
Middle Ear Effusion ◽  
Middle Ear Mucosa ◽  
Immunohistochemical Technique ◽  
External Canal ◽  
Human Middle Ear ◽  
Ear Effusion

Prostaglandins were found in the middle ear effusion and may play an important role in inflammation and bone resorption occurring in otitis media. With the use of an immunohistochemical technique, prostaglandin cyclooxygenase was localized in epithelial cells of human skin, endothelial cells of arterioles, mononuclear leukocytes and fibroblasts of human middle ear mucosa and granulation tissue, epithelial cells of chinchilla middle ear mucosa, and the external auditory canal skin.

Basement Membrane in Middle Ear Cholesteatoma Immunohistochemical and Ultrastructural Observations

1996 ◽  
Vol 105 (10) ◽  
pp. 804-810 ◽  
Author(s):  
Jesus Bujía ◽  
Anja Holly ◽  
Holger Sudhoff ◽  
Gerd Borkowski ◽  
Henning Hildmann ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Middle Ear ◽  
Collagen Type ◽  
Collagen Type Iv ◽  
Middle Ear Mucosa ◽  
Type Iv ◽  
Middle Ear Cholesteatoma ◽  
Human Middle Ear ◽  
Cell Matrix Interactions ◽  
Collagen Type Vii

We investigated the distribution of basement membrane zone (BMZ) components collagen type IV, collagen type VII, and fibronectin in human middle ear cholesteatoma, auditory meatal skin, and middle ear mucosa using both immunohistochemical and ultrastructural methods. Collagen type IV inununoreactivity of skin and middle ear mucosa is continuous in the BMZ, whereas cholesteatoma frequently showed absent immunoreactivity or focal discontinuities. Collagen type VII inununoreactivity is detected similarly within the BMZ of cholesteatoma and skin. Fibronectin immunoreactivity is observed within the dermoepithelial junction of skin and middle ear mucosa. In cholesteatoma, however, fibronectin immunoreactivity is markedly increased within the extrinsic BMZ and the subepithelial connective tissue. The ultrastructural arrangement of the BMZ of cholesteatoma is like that of skin; however, it exhibits distinct alterations of the lamina fibroreticularis and lamina densa. Our results outline cholesteatoma as a disease with disturbed cell matrix interactions analogous to those of wound reepithelialization.

Secretory Lysozyme of the Human Middle Ear Mucosa: Immunocytochemical Localization

1976 ◽  
Vol 85 (1) ◽  
pp. 50-60 ◽  
Author(s):  
David J. Lim ◽  
Yea S. Liu ◽  
Herbert Birck
Keyword(s):  
Middle Ear ◽  
Secretory Cells ◽  
Immunocytochemical Localization ◽  
Biochemical Finding ◽  
Middle Ear Mucosa ◽  
Specific Granules ◽  
Neutrophilic Leukocytes ◽  
Morphological Finding ◽  
Human Middle Ear ◽  
Serous Otitis Media

Lysozyme was demonstrated by an immunocytochemical technique in the biopsied mucosa obtained from the promontory of the fifteen patients who had chronic middle ear effusions. Lysozyme was localized in the mucigen granules of the secretory cells, as well as in the specific granules of the polymorphonuclear neutrophilic leukocytes (PMN) and macrophages. The specimens obtained from patients with mucous effusions showed numerous secretory cells that contained lysozyme, in sharp contrast to the serous type in which only a few secretory cells could be found. The present morphological finding was in agreement with the biochemical finding which demonstrated higher lysozyme level in mucous effusions than that of the serous type. It was concluded that human middle ear mucosa provided lysozyme and that its secretion was active in serous otitis media, particularly of mucoid type.

Characterization of mucins in human middle ear and Eustachian tube

2001 ◽  
Vol 280 (6) ◽  
pp. L1157-L1167 ◽  
Author(s):  
Jizhen Lin ◽  
Vladimir Tsuprun ◽  
Hirokazu Kawano ◽  
Michael M. Paparella ◽  
Zhiqiang Zhang ◽  
...  
Keyword(s):  
Otitis Media ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Middle Ear Mucosa ◽  
Pathological Conditions ◽  
Mucin Expression ◽  
Mucin Genes ◽  
Human Middle Ear

Mucins are important glycoproteins in the mucociliary transport system of the middle ear and Eustachian tube. Little is known about mucin expression within this system under physiological and pathological conditions. This study demonstrated the expression of MUC5B, MUC5AC, MUC4, and MUC1 in the human Eustachian tube, whereas only MUC5B mucin expression was demonstrated in noninflamed middle ears. MUC5B and MUC4 mucin genes were upregulated 4.2- and 6-fold, respectively, in middle ears with chronic otitis media (COM) or mucoid otitis media (MOM). This upregulation of mucin genes was accompanied by an increase of MUC5B- and MUC4-producing cells in the middle ear mucosa. Electron microscopy of the secretions from COM and MOM showed the presence of chainlike polymeric mucin. These data indicate that the epithelium of the middle ear and Eustachian tube expresses distinct mucin profiles and that MUC5B and MUC4 mucins are highly produced and secreted in the diseased middle ear. These mucins may form thick mucous effusion in the middle ear cavity and compromise the function of the middle ear.

Abnormalities of Cilia in the Middle Ear in Chronic Otitis Media

1988 ◽  
Vol 97 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Herman Yeger ◽  
Erma Minaker ◽  
Allen Rubin ◽  
David Charles ◽  
Jennifer M. Sturgess
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Mucociliary Clearance ◽  
Matched Control ◽  
Chronic Otitis Media ◽  
Middle Ear Mucosa ◽  
Tissue Samples ◽  
Clinical Complications ◽  
Nasal Cilia ◽  
Abnormal Cilia

We studied the fine structure of nasal and middle ear mucosa in 18 patients with chronic otitis media, comparing them with ten age-matched control patients. Electron microscopy revealed no single fundamental defect of cilia in the tissue samples; however, patients with chronic otitis media had a significantly higher incidence of abnormal cilia in the middle ear without correlating abnormalities of nasal cilia. These findings indicated that ciliary anomalies, including deletion of ciliary microtubules, presence of supernumerary tubules, and fusion of cilia, occur as a result of infection or inflammation. Such abnormalities would impair normal mucociliary clearance and exacerbate clinical complications.

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