scholarly journals Comparison of an Automated Fluorimetric Method with Two Manual Methods for the Determination of Pregnancy Oestrogens

1972 ◽  
Vol 9 (1-6) ◽  
pp. 141-142
Author(s):  
G. G. Muir ◽  
P. Scott ◽  
M. Ryan ◽  
V. Bone
Keyword(s):  
Intermittent Demand ◽  
Fluorimetric Method ◽  
Automated Method ◽  
Manual Methods

Three methods of determining urinary oestrogens are compared. Manual methods are better for intermittent demand but an automated method is desirable when more than 50 analyses per week are required.

Determination of Uric Acid

1964 ◽  
Vol 10 (9) ◽  
pp. 838-844 ◽  
Author(s):  
Leonard V Crowley
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Blood Sugar ◽  
Protein Precipitation ◽  
Automated Method ◽  
Manual Methods

Abstract An automated method is described for the determination of uric acid by a carbonate method. Since uric acid is separated from proteins by dialysis, the loss of uric acid due to protein precipitation in manual methods is avoided. There is no appreciable interference from salicylates, high levels of blood sugar, or ascorbic acid. The method shows an acceptable correlation with the uricase method.

Automated Quantitative Immunochemical Analysis of Human Immunoglobulins

1972 ◽  
Vol 18 (11) ◽  
pp. 1364-1367 ◽  
Author(s):  
Harold Markowitz ◽  
A R Tschida
Keyword(s):  
Continuous Flow ◽  
Serum Proteins ◽  
Immunochemical Analysis ◽  
Cerebrospinal Fluid Proteins ◽  
Automated Method ◽  
Specific Serum ◽  
Human Immunoglobulins ◽  
Precise Estimation ◽  
Manual Methods

Abstract A commercial automated continuous-flow procedure for estimating specific serum proteins has been evaluated for use in determination of IgG, IgA, IgM (both 19S and 7S), α1-antitrypsin, and cerebrospinal fluid proteins. Results of the automated procedure correlate well with manual methods, but are much more precise. Estimation of 7S IgM by the automated method is more accurate than estimation by radial immunodiffusion, because concentrations of 7S IgM are not spuriously elevated.

Automated Simultaneous Turbidimetric Determination of Cholesterol in β- and pre-β-Lipoproteins

1971 ◽  
Vol 17 (10) ◽  
pp. 994-997 ◽  
Author(s):  
A Lopez ◽  
R Vial ◽  
L Gremillion ◽  
L Bell
Keyword(s):  
Mass Screening ◽  
Accurate Method ◽  
Automated Method ◽  
Manual Methods

Abstract An automated method is described for simultaneously determining cholesterol in serum β- and pre-β-lipoprotein fractions. This fast, reproducible, accurate method depends on the ability of β- and pre-β-lipoproteins to form insoluble complexes with polyanionic polysaccharides such as heparin. Values obtained by this method and by similar manual methods described in the literature agree well. This method is extremely useful in mass screening for hyperlipidemias; in certain cases it may be more useful than the determination of only cholesterol or triglycerides.

An Automated Micromethod for Determination of Serum Glucose, with an Improved o-Toluidine Reagent

1970 ◽  
Vol 16 (4) ◽  
pp. 285-290 ◽  
Author(s):  
Wells R Moorehead ◽  
Edward A Sasse
Keyword(s):  
Life Expectancy ◽  
Polyvinyl Chloride ◽  
Coefficient Of Variation ◽  
Serum Glucose ◽  
Automated Method ◽  
Beer's Law ◽  
Beer’S Law ◽  
Manual Methods ◽  
Reducing Properties

Abstract An automated method has been developed in which a new o-toluidine reagent is used for the microanalysis of glucose. The reagent is 1.3 times as sensitive as the o-toluidine reagent commonly used in manual procedures. The reagent does not significantly alter the life expectancy of manifold tubing made of polyvinyl chloride. The automation includes a dialysis step and possesses the desirable features of the manual methods, including the greater specificity as compared with methods based on reducing properties. Results of this automated method obey Beer’s law, are very reproducible (the coefficient of variation is approximately 1.5%), and are not significantly different from those obtained manually. The method requires 75 µl of sample.

Automated Fluorometric Determination of Thiamine and Riboflavin in Infant Formulas

1979 ◽  
Vol 62 (3) ◽  
pp. 642-647
Author(s):  
Walter E Dunbar ◽  
Kenneth E Stevenson
Keyword(s):  
Sample Preparation ◽  
Infant Formulas ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Automated Method ◽  
Coefficients Of Variation ◽  
Manual Methods ◽  
The Mean ◽  
Better Than

Abstract Commercial infant formulas were analyzed simultaneously for thiamine and riboflavin by an automated fluorometric method and by the AOAC manual fluorometric methods. For 10 products, the mean thiamine and riboflavin results determined using the automated method ranged from 104 to 113% and 90 to 112%, respectively, of those by the AOAC manual methods. The coefficients of variation for thiamine and riboflavin ranged from 1.05 to 3.90% and 0.60 to 2.48%, respectively, for the automated method, and 1.48 to 3.86% and 0.69 to 10.9%, respectively, for the manual methods. Using the automated method, mean recoveries of thiamine and riboflavin added to samples were 103 and 104%, respectively. The automated method used a common sample preparation to determine both thiamine and riboflavin, and gave results equivalent to, or better than, those obtained by the manual methods.

An Automated Fluorometric Method for Determination of Lactic Dehydrogenase in Serum

1965 ◽  
Vol 11 (8) ◽  
pp. 748-762 ◽  
Author(s):  
Lewis Brooks ◽  
Harry G Olken
Keyword(s):  
Enzyme Activity ◽  
Incubation Time ◽  
Lactic Dehydrogenase ◽  
Fluorometric Method ◽  
Automated Method ◽  
Ldh Activity ◽  
Manual Methods

Abstract A simple automated method for the determination of lactic dehydrogenase (LDH) activity in serum by the forward lactate → pyruvate reaction is presented. Enzyme activity is determined by measuring the fluorescence of DPNH formed after a fixed incubation time. Detailed studies of various parameters of the reaction, reproducibility, and recovery data, and a comparison with two manual methods for LDH are presented.

An Automated Procedure for the Simultaneous Determination of Calcium and Phosphorus

1964 ◽  
Vol 10 (8) ◽  
pp. 686-703 ◽  
Author(s):  
Gerald Kessler ◽  
Morris Wolfman
Keyword(s):  
Simultaneous Determination ◽  
Inorganic Phosphorus ◽  
Sample Treatment ◽  
Automated Method ◽  
Test Conditions ◽  
Individual Determination ◽  
Manual Methods ◽  
Calcium And Phosphorus ◽  
Serum And Urine

Abstract A completely automated method for the simultaneous or individual determination of calcium and phosphorus in serum and urine without preliminary sample treatment is reported. Separation of calcium and phosphorus from interfering materials is achieved by dialysis under acid conditions. The dialyzed calcium is determined colorimetrically using a metal complexing dye (cresolphthalein complexone) in an alkaline medium. Magnesium, phosphate, citrate, sulfate, etc., did not introduce significant errors under the test conditions. Inorganic phosphorus is determined by a modified Fiske-SubbaRow procedure. Reproducibility and recovery data as well as comparisons with manual methods are presented.

Sign in / Sign up

Export Citation Format

Share Document