scholarly journals Immunoturbidimetry of Serum C-Reactive Protein in Low Concentration of Polyethylene Glycol

1985 ◽  
Vol 22 (3) ◽  
pp. 269-272 ◽  
Author(s):  
I Byrjalsen ◽  
S H Ingwersen
Keyword(s):  
Polyethylene Glycol ◽  
Radial Immunodiffusion ◽  
C Reactive Protein ◽  
Low Concentration ◽  
Reactive Protein ◽  
Polyethylene Glycol 6000

A simple, rapid immunoturbidimetric assay for determination of serum C-reactive protein is presented. In order to obtain constant sample blank values within a few minutes we found it necessary to use a low concentration (10 g/L) of polyethylene glycol 6000 in contrast to the commonly used 40 g/L PEG solution. The sensitivity of the assay presented is 7 mg/L; within-run and between-run CVs ranged respectively from 1·4% to 2·4% and from 2·1% to 5·5%. Results obtained with the proposed method correlate well with radial immunodiffusion.

Turbidimetric immunoassay of serum C-reactive protein.

1982 ◽  
Vol 28 (10) ◽  
pp. 2121-2124 ◽  
Author(s):  
S Otsuji ◽  
H Shibata ◽  
M Umeda
Keyword(s):  
Radial Immunodiffusion ◽  
Tween 20 ◽  
C Reactive Protein ◽  
Standard Curve ◽  
Reactive Protein ◽  
Analytical Recovery ◽  
Polyethylene Glycol 6000 ◽  
Sample Dilution ◽  
Immunoprecipitation Reaction ◽  
Turbidimetric Immunoassay

Abstract This rapid, reliable equilibrium turbidimetric immunoassay for serum C-reactive protein involves a potent monospecific antibody. Polyethylene glycol-6000 to accelerate and enhance the immunoprecipitation reaction, and Tween-20 surfactant to lower and stabilize the sample blank values. Grossly lipemic, icteric, or hemolyzed sera can be assayed. Values up to about 220 mg/L, for which the standard curve is linear, can be measured without sample dilution. Results by the proposed method and by radial immunodiffusion (r 0.989) or laser nephelometry (r = 0.957) correlated well. Analytical recovery averaged 101.3%. Within-, between-, and day-to-day CVs ranged from 0.9% to 3.5%, 0.8% to 5.5%, and 1.9% to 4.8%, respectively. The method is demonstrably superior to radial immunodiffusion or nephelometry. Any spectrophotometer that can measure turbidimetrically at 340 nm can be used.

C-REACTIVE PROTEIN IN THE SERUM IN NEPHROSIS AND ACUTE GLOMERULONEPHRITIS

PEDIATRICS ◽  
1960 ◽  
Vol 25 (1) ◽  
pp. 106-111
Author(s):  
Aree Valyasevi ◽  
Joseph M. Sloan ◽  
Lewis A. Barness
Keyword(s):  
Nephrotic Syndrome ◽  
Hormone Therapy ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Positive Test ◽  
Acute Glomerulonephritis ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation

C-reactive protein in the serum and the erythrocyte sedimentation rate were followed serially in 13 patients with acute nephritis and in 9 patients with the nephrotic syndrome. Although a positive C-reactive protein test was always associated with evidence of infection, obvious infection in a few instances was not accompanied by a positive test. Determination of C-reactive protein is valuable in determining the presence of infection in patients with acute nephritis and the nephrotic syndrome, especially in the latter where hormone therapy may mask many signs of infection.

Comparison of Three Commercial Assays for the Determination of Serum C-Reactive Protein

1999 ◽  
Vol 7 (2) ◽  
pp. 45-59
Author(s):  
TARIF ZAWAWI ◽  
IBRAHTM HASHIM ◽  
WALEED AL-YAFI ◽  
MOHAMMED ABDELLAAL
Keyword(s):  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Stimulation of Erythrocyte Cell Membrane Scrambling by C-Reactive Protein

2017 ◽  
Vol 41 (2) ◽  
pp. 806-818 ◽  
Author(s):  
Majed Abed ◽  
Christian Thiel ◽  
Syeda T. Towhid ◽  
Kousi Alzoubi ◽  
Sabina Honisch ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Healthy Volunteers ◽  
Annexin V ◽  
Caspase Activation ◽  
C Reactive Protein ◽  
Forward Scatter ◽  
Reactive Protein ◽  
Phosphatidylserine Translocation ◽  
Binding Cell

Background: Eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine-translocation, is triggered by fever and inflammation. Signaling includes increased cytosolic Ca2+-activity ([Ca2+]i), caspase activation, and ceramide. Inflammation is associated with increased plasma concentration of C-reactive protein (CRP). The present study explored whether CRP triggers eryptosis. Methods: Phosphatidylserine abundance at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, ceramide abundance and caspase-3-activity utilizing FITC-conjugated antibodies. Moreover, blood was drawn from patients with acute appendicitis (9♀,11♂) and healthy volunteers (10♀,10♂) for determination of CRP, blood count and phosphatidylserine. Results: A 48h CRP treatment significantly increased the percentage of annexin-V-binding cells (≥5µg/ml), [Ca2+]i (≥5µg/ml), ceramide (20µg/ml) and caspase-activity (20µg/ml). Annexin-V-binding was significantly blunted by caspase inhibitor zVAD (10µM). The percentage of phosphatidylserine-exposing erythrocytes in freshly drawn blood was significantly higher in appendicitis patients (1.83±0.21%) than healthy volunteers (0.81±0.09%), and significantly higher following a 24h incubation of erythrocytes from healthy volunteers to patient plasma than to plasma from healthy volunteers. The percentage of phosphatidylserine-exposing erythrocytes correlated with CRP plasma concentration. Conclusion: C-reactive protein triggers eryptosis, an effect at least partially due to increase of [Ca2+]i, increase of ceramide abundance and caspase activation.

Rapid quantification of C-reactive protein by centrifugal analysis.

1983 ◽  
Vol 29 (4) ◽  
pp. 696-697 ◽  
Author(s):  
L Melamies
Keyword(s):  
Reaction Time ◽  
Normal Values ◽  
Regression Equation ◽  
Radial Immunodiffusion ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Coefficients Of Variation ◽  
Quantitative Results ◽  
Rapid Quantification

Abstract I describe a rapid, simple immunoturbidimetric method for determining C-reactive protein in serum. With the Instrumentation Laboratory Multistat III microcentrifugal analyzer, quantitative results are obtained automatically after a few minutes of reaction time. Within-run and between-run coefficients of variation ranged from 2.5 to 12.7% at C-reactive protein concentrations of 55 to 69 and 14 to 25 mg/L, respectively, normal values being less than 10 mg/L. Comparison with the commercially available radial immunodiffusion method (y) yields the regression equation y = 1.011x - 2.112 (r = 0.979, n = 100).

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