scholarly journals Serum Protein Zone Electrophoresis—An Outmoded Test?

1987 ◽  
Vol 24 (2) ◽  
pp. 133-139 ◽  
Author(s):  
J T Whicher ◽  
C E Spence
Keyword(s):  
Serum Protein ◽  
Plasma Protein ◽  
Predictive Value ◽  
Laboratory Studies ◽  
Zone Electrophoresis ◽  
Protein Zone ◽  
Protein Measurement

The predictive value of plasma protein changes in disease is very largely unknown, as relevant clinical and laboratory studies are lacking. The usefulness of zone electrophoresis as a method of detecting a constellation of plasma protein changes is even less clear. With the advent of precise automated techniques for specific plasma protein measurement, zone electrophoresis has little to offer except in the identification and quantitation of paraproteins, where it is essential.

scholarly journals Predictive value of pregnancy-associated plasma protein-A in relation to fetal loss: A systematic review and meta-analysis

2020 ◽  
Author(s):  
Zahra Hadizadeh-Talasaz ◽  
Ali Taghipour ◽  
Seyede Houra Mousavi-Vahed ◽  
Robab Latifnejad Roudsari
Keyword(s):  
Systematic Review ◽  
Likelihood Ratio ◽  
Plasma Protein ◽  
Predictive Value ◽  
Meta Analysis ◽  
Protein A ◽  
Fetal Loss ◽  
Positive Likelihood Ratio ◽  
Negative Likelihood Ratio ◽  
Forest Plot

Background: For a woman with bleeding and threatened abortion, ultrasound scan is done to confirm the viability of the fetus; however, 10-15% of the embryos are eventually aborted. Distinguishing between women with good and poor prognosis can be a helpful approach. Objective: This study aimed to review the predictive value of Pregnancy-associated Plasma Protein A (PAPP-A) in relation to the diagnosis of fetal loss. Materials and Methods: The articles published in multiple databases including Web of Science, PubMed, MEDLINE, Scopus, and Persian databases such as ISC, Magiran, and IranMedx were searched for articles published until May 2019. MeSH terms was used for searching the databases including fetal loss OR pregnancy loss OR abortion OR miscarriage with the following word using AND; Pregnancy-Associated Plasma Protein- A OR PAPP-A. Two reviewers extracted data and recorded them in a pre-defined form and assessed the quality of articles using the Newcastle-Ottawa tool. Meta-analysis was done using the Comprehensive Meta-Analysis/2.0 software and MetaDisc. Results: A total number of 16 studies were eligible for the qualitative data synthesis, out of which 8 studies were included in the meta-analysis. All studies had high and medium quality. The forest plot analysis showed a sensitivity of 57% (95% CI: 53-63%), a specificity of 83% (95% CI: 80-85%), a positive likelihood ratio of 3.52 (95% CI: 2.44- 5.07), a negative likelihood ratio of 0.54 (95% CI: 0.37-0.79), and a diagnostic odds ratio of 6.95 (95% CI: 3.58-13.50). Conclusion: PAPP-A cannot be recommended on a routine basis for predicting fetal loss and still further research with a combination of other biomarkers is required. Key words: Pregnancy-associated plasma protein-A, Fetal loss, Pregnancy, Systematic review.

Case report: Interference from isatuximab on serum protein electrophoresis prevented demonstration of complete remission in a myeloma patient

2021 ◽  
pp. 000456322110620
Author(s):  
Rachel D Wheeler ◽  
Micsha V Costa ◽  
Asante Crichlow ◽  
Fenella Willis ◽  
Yasmin Reyal ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Complete Remission ◽  
Capillary Zone Electrophoresis ◽  
Serum Protein ◽  
Plasma Cells ◽  
Survival Rates ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone

Multiple myeloma is a haematological cancer caused by malignant plasma cells in the bone marrow that can result in organ dysfunction and death. Recent novel treatments have contributed to improved survival rates, including monoclonal antibody therapies that target the CD38 protein on the surface of plasma cells. Anti-CD38 therapies are IgG kappa monoclonal antibodies that are given in doses high enough for the drug to be visible on serum protein electrophoresis as a small paraprotein. We present a case where isatuximab, the most recent anti-CD38 monoclonal antibody to be approved for treatment of myeloma, obscured the patient’s paraprotein on gel immunofixation, so that complete remission could not be demonstrated. This was resolved using the isatuximab Hydrashift assay. The interference on gel immunofixation was unexpected because isatuximab migrated in a position distinct from the patient’s paraprotein on capillary zone electrophoresis. We demonstrate the surprising finding that isatuximab migrates in a different position on gel electrophoresis compared to capillary zone electrophoresis. It is vital that laboratories are aware of the possible interference on electrophoresis from anti-CD38 monoclonal antibody therapies, and are able to recognise these drugs on protein electrophoresis. The difference in isatuximab’s electrophoretic mobility on capillary and gel protein electrophoresis makes this particularly challenging. Laboratories should have a strategy for alternative analyses in the event that the drugs interfere with assessment of the patient’s paraprotein.

scholarly journals Identification of four novel serum protein biomarkers in sepsis patients encoded by target genes of sepsis-related miRNAs

2014 ◽  
Vol 126 (12) ◽  
pp. 857-867 ◽  
Author(s):  
Hui-juan Wang ◽  
Bao-zeng Wang ◽  
Peng-jun Zhang ◽  
Jie Deng ◽  
Zhi-rui Zhao ◽  
...  
Keyword(s):  
Serum Protein ◽  
Sofa Score ◽  
Predictive Value ◽  
Mirna Target ◽  
Target Genes ◽  
Apache Ii Score ◽  
Protein Biomarkers ◽  
Combined Analysis ◽  
Better Than ◽  
Novel Protein

Four novel protein biomarkers encoded by the miRNA target genes were identified for patients with sepsis. The combined analysis of the four proteins indicated that their predictive value for sepsis prognosis was better than the values for the SOFA score and APACHE II score.

ZONE ELECTROPHORESIS OF THE PROTEINS OF THE FOWL'S SERUM AND EGG YOLK

1959 ◽  
Vol 37 (12) ◽  
pp. 1457-1468 ◽  
Author(s):  
K. A. McCully ◽  
W. A. Maw ◽  
R. H. Common
Keyword(s):  
Saline Solution ◽  
Laying Hens ◽  
Egg Yolk ◽  
Yolk Protein ◽  
Protein Fractions ◽  
Zone Electrophoresis ◽  
The Status ◽  
Minor Protein ◽  
Protein Zone

In addition to the six protein fractions distinguishable in sera from cocks or sexually immature pullets by zone electrophoresis in aqueous veronal, two lipoprotein zones have now been distinguished in sera from laying hens or estrogenized pullets by this same technique. Phosvitin has been detected in serum of estrogenized hens by zone electrophoresis of a saline solution of crude serum lipovitellin obtained from the serum. Six protein zones have been distinguished in similar electropherograms of egg yolk. The six protein zones have been identified provisionally, and in order of decreasing mobility under the conditions used, as phosvitin, α-livetin, β-livetin, γ-livetin, lipoprotein P-1 (possibly a lipovitellenin complex), and lipoprotein P-2 (possibly a lipovitellin complex). Evidence is submitted that the foregoing two major lipoprotein zones from egg yolk are closely similar to, or identical with, the two lipoprotein zones from sera of laying hens or estrogenized pullets. The status of a seventh minor protein zone in electropherograms of yolk protein is uncertain.

ZONE ELECTROPHORESIS OF THE PROTEINS OF THE FOWL'S SERUM AND EGG YOLK

1959 ◽  
Vol 37 (1) ◽  
pp. 1457-1468 ◽  
Author(s):  
K. A. McCully ◽  
W. A. Maw ◽  
R. H. Common
Keyword(s):  
Saline Solution ◽  
Laying Hens ◽  
Egg Yolk ◽  
Yolk Protein ◽  
Protein Fractions ◽  
Zone Electrophoresis ◽  
The Status ◽  
Minor Protein ◽  
Protein Zone

In addition to the six protein fractions distinguishable in sera from cocks or sexually immature pullets by zone electrophoresis in aqueous veronal, two lipoprotein zones have now been distinguished in sera from laying hens or estrogenized pullets by this same technique. Phosvitin has been detected in serum of estrogenized hens by zone electrophoresis of a saline solution of crude serum lipovitellin obtained from the serum. Six protein zones have been distinguished in similar electropherograms of egg yolk. The six protein zones have been identified provisionally, and in order of decreasing mobility under the conditions used, as phosvitin, α-livetin, β-livetin, γ-livetin, lipoprotein P-1 (possibly a lipovitellenin complex), and lipoprotein P-2 (possibly a lipovitellin complex). Evidence is submitted that the foregoing two major lipoprotein zones from egg yolk are closely similar to, or identical with, the two lipoprotein zones from sera of laying hens or estrogenized pullets. The status of a seventh minor protein zone in electropherograms of yolk protein is uncertain.

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