scholarly journals Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering

2011 ◽  
Vol 23 (3) ◽  
pp. 325-332 ◽  
Author(s):  
V.T. Sakai ◽  
M.M. Cordeiro ◽  
Z. Dong ◽  
Z. Zhang ◽  
B.D. Zeitlin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Dental Pulp ◽  
Vascular Endothelial Cells ◽  
Dental Pulp Stem Cells ◽  
Vascular Endothelial ◽  
Pulp Tissue ◽  
Differentiation Potential ◽  
Dental Pulp Tissue

Multipotency is a defining characteristic of post-natal stem cells. The human dental pulp contains a small subpopulation of stem cells that exhibit multipotency, as demonstrated by their ability to differentiate into odontoblasts, neural cells, and vascular endothelial cells. These discoveries highlight the fundamental role of stem cells in the biology of the dental pulp and suggest that these cells are uniquely suited for dental pulp tissue-engineering purposes. The availability of experimental approaches specifically designed for studies of the differentiation potential of dental pulp stem cells has played an important role in these discoveries. The objective of this review is to describe the development and characterization of the Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering. In addition, we discuss the multipotency of dental pulp stem cells, focusing on the differentiation of these cells into functional odontoblasts and into vascular endothelial cells.

scholarly journals VEGFR1 primes a unique cohort of dental pulp stem cells for vasculogenic differentiation

2021 ◽  
Vol 41 ◽  
pp. 332-344
Author(s):  
MT Bergamo ◽  
◽  
Z Zhang ◽  
TM Oliveira ◽  
JE Nör
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Vascular Endothelial Cells ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
Microvascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Pulp Tissue ◽  
Immunodeficient Mice

Dental pulp stem cells (DPSCs) constitute a unique group of cells endowed with multipotency, self-renewal, and capacity to regenerate the dental pulp tissue. While much has been learned about these cells in recent years, it is still unclear if each DPSC is multipotent or if unique sub-populations of DPSCs are “primed” to undergo specific differentiation paths. The purpose of the present study was to define whether a sub-population of DPSCs was uniquely primed to undergo vasculogenic differentiation. Permanent-tooth DPSCs or stem cells from human exfoliated deciduous teeth (SHED) were flow-sorted for vascular endothelial growth factor receptor 1 (VEGFR1) and exposed to vasculogenic differentiation medium, i.e., Microvascular-Endothelial-Cell-Growth-Medium-2-BulletKit™ supplemented with 50 ng/mL rhVEGF165 in the presence of 0 or 25 μg/mL anti-human VEGF antibody (bevacizumab; Genentech). In addition, sorted SHED (i.e., VEGFR1high or VEGFR1low) were seeded in biodegradable scaffolds and transplanted into the subcutaneous space of immunodeficient mice. Despite proliferating at a similar rate, VEGFR1high generated more in vitro sprouts than VEGFR1low cells (p < 0.05). Blockade of VEGF signaling with bevacizumab inhibited VEGFR1high-derived sprouts, demonstrating specificity of responses. Similarly, VEGFR1high SHED generated more blood vessels when transplanted into murine hosts than VEGFR1low cells (p < 0.05). Collectively, these data demonstrated that DPSCs contain a unique sub-population of cells defined by high VEGFR1 expression that are primed to differentiate into vascular endothelial cells. These data raise the possibility of purifying stem cells with high vasculogenic potential for regeneration of vascularized tissues or for vascular engineering in the treatment of ischemic conditions.

scholarly journals Dental Pulp Tissue Regeneration Using Dental Pulp Stem Cells Isolated and Expanded in Human Serum

2017 ◽  
Vol 43 (4) ◽  
pp. 568-574 ◽  
Author(s):  
Evandro Piva ◽  
Susan A. Tarlé ◽  
Jacques E. Nör ◽  
Duohong Zou ◽  
Elizabeth Hatfield ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Serum ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Tissue Regeneration

Dental Pulp Tissue Engineering Using Mesenchymal Stem Cells: a Review with a Protocol

2018 ◽  
Vol 14 (5) ◽  
pp. 668-676 ◽  
Author(s):  
Tomoatsu Kaneko ◽  
Bin Gu ◽  
Phyo Pyai Sone ◽  
Su Yee Myo Zaw ◽  
Hiroki Murano ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Pulp Tissue

Dental pulp tissue engineering of pulpotomized rat molars with bone marrow mesenchymal stem cells

Odontology ◽  
2016 ◽  
Vol 105 (4) ◽  
pp. 392-397 ◽  
Author(s):  
Takafumi Ito ◽  
Tomoatsu Kaneko ◽  
Yukiko Sueyama ◽  
Reika Kaneko ◽  
Takashi Okiji
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Marrow Mesenchymal Stem Cells ◽  
Dental Pulp Tissue ◽  
Rat Molars

scholarly journals Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy

Biology ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 160 ◽  
Author(s):  
Shinichiro Yoshida ◽  
Atsushi Tomokiyo ◽  
Daigaku Hasegawa ◽  
Sayuri Hamano ◽  
Hideki Sugii ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
High Growth ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Pulp Tissue ◽  
Differentiation Potential ◽  
Cell Based Therapy

Mesenchymal stem cells (MSCs) have the capacity for self-renewal and multilineage differentiation potential, and are considered a promising cell population for cell-based therapy and tissue regeneration. MSCs are isolated from various organs including dental pulp, which originates from cranial neural crest-derived ectomesenchyme. Recently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) have been isolated from dental pulp tissue of adult permanent teeth and deciduous teeth, respectively. Because of their MSC-like characteristics such as high growth capacity, multipotency, expression of MSC-related markers, and immunomodulatory effects, they are suggested to be an important cell source for tissue regeneration. Here, we review the features of these cells, their potential to regenerate damaged tissues, and the recently acquired understanding of their potential for clinical application in regenerative medicine.

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