In Vitro Interactions Between the Surfaces of Enamel White Spots and Calcifying Solutions

1976 ◽  
Vol 55 (1) ◽  
pp. 135-141 ◽  
Author(s):  
Stephen H.Y. Wei ◽  
James S. Wefel
Keyword(s):  
Electron Microscope ◽  
Calcium Phosphate ◽  
White Spot ◽  
Reaction Products ◽  
High Concentration ◽  
White Spots ◽  
Scanning Electron ◽  
Spot Formation ◽  
In Vitro Interactions

The morphological appearance of white spot enamel after treatment with various calcium phosphate and sodium fluoride solutions was examined using the scanning electron microscope. The reaction products formed after the sequential application of the calcifying solutions and a high concentration of fluoride appeared to fill in the porosity created in the enamel surface by white spot formation.

Use of Laser fluorescence and Scanning Electron Microscope to Evaluate Remineralization of Incipient Enamel Lesions Remineralized by Topical Application of Casein Phospho Peptide Amorphous Calcium Phosphate (CPP-ACP) Containing Cream

2008 ◽  
Vol 32 (3) ◽  
pp. 201-206 ◽  
Author(s):  
Deepika. Pai ◽  
Sham Bhat ◽  
Abhay Taranath ◽  
Sharan Sargod ◽  
Vinita Pai
Keyword(s):  
Electron Microscope ◽  
Calcium Phosphate ◽  
Scanning Electron Microscope ◽  
Topical Application ◽  
Amorphous Calcium Phosphate ◽  
In Vitro Study ◽  
Laser Fluorescence ◽  
Casein Phosphopeptide ◽  
Scanning Electron

The aim of this in vitro study was to evaluate the remineralization of incipient enamel lesions by the topical application of Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP) using laser fluorescence and scanning electron microscope. Sixty caries free extracted teeth were used in the study. Forty teeth were used as test samples, ten as positive and ten as negative controls. The samples were demineralized and then remineralized by the topical application of CPP-ACP for a period of 14 days. The remineralization was evaluated with the use of laser fluorescence and scanning electron microscope (SEM). The results of this study showed that the laser fluorescent readings of test samples after remineralization were highly significant (p <0.001). A significant number of test samples observed under SEM showed high scores of remineralization.

Effect of Sr on the Bioactivity of Sol-Gel Derived New Silicate Bioglass S55P4

2020 ◽  
Vol 1010 ◽  
pp. 613-619
Author(s):  
Thet Swe Thet ◽  
Hasmaliza M. Mohamad ◽  
Khairul Anuar Shariff
Keyword(s):  
Electron Microscope ◽  
Calcium Phosphate ◽  
Sol Gel ◽  
Apatite Formation ◽  
Sem Images ◽  
X Ray ◽  
Low Crystalline ◽  
Crystalline Peak ◽  
Scanning Electron

Strontium (Sr) stimulates osteoblast and inhibits osteoclast activities in-vitro and is used clinically as a treatment for osteoporosis. In this research, the effect of Sr substitution on the apatite formation of sol-gel derived bioactive glass (BG) (55.90SiO2-1.72P2O5 -21.67Na2O - (20.69-x) CaO -x SrO) (x=0, 5 and 8 mol. %) were investigated. The synthesized Sr doped BG samples were treated in Hank's Balanced Salt Solution (HBSS) for 14 days to study the bioactivity. The achieved samples were evaluated by X-ray powder diffraction (XRD) and Scanning electron microscope (SEM). In XRD, the hydroxyapatite (HA) crystalline peak for 8% Sr-BG is less compared with others. When Sr amount is increased to 8%, the low crystalline peaks of HA were detected although the same soaking duration. FTIR spectra supported the delay precipitation of calcium phosphate (CaP), especially for the specimen containing 8% Sr. After 14 days soaking, SEM images confirmed the bioactivity of the synthesized samples by the formation of apatite on the glass surface.

scholarly journals Caries-preventive Efficacy of Resin Infiltrant, Casein Phosphopeptide-amorphous Calcium Phosphate, and Nanohydroxyapatite using Confocal Scanning Electron Microscope: An in vitro Study

2017 ◽  
Vol 2 (1) ◽  
pp. 6-10
Author(s):  
A Arvindkumar ◽  
Uma Maheswari ◽  
C Kingston ◽  
R Gnanaseelan ◽  
R Jonathan Emil Sam
Keyword(s):  
Electron Microscope ◽  
Calcium Phosphate ◽  
Group Iv ◽  
Group Iii ◽  
Group I ◽  
Preventive Efficacy ◽  
Confocal Scanning ◽  
Casein Phosphopeptide ◽  
Scanning Electron

ABSTRACT Introduction White spot lesion is the early sign of demineralization occurring under intact enamel which may lead to the development of caries. Aim The aim of this study is to compare and evaluate the caries-preventive efficacy of a resin infiltrant, casein phosphopeptideamorphous calcium phosphate (CPP-ACP), and nanohydroxyapatite (nano-HA) on white spot enamel lesions. Settings and designs A total of 40 freshly extracted human maxillary incisors were used in this study. Enamel samples (2 mm thickness) were prepared and sample preparation windows were created (dimension of 5 × 5 mm) using adhesive tape, and the sample was made completely resistant to acid attack by coating nail varnish. Materials and methods The samples were divided into four groups, which are resin infiltrant, CPP-ACP, nano-HA, and control of 10 enamel samples in each group. The samples were evaluated using confocal laser scanning microscopy before and after the application of resin infiltrant and remineralizing agents. Statistical analysis Statistical analysis was done using analysis of variance and post hoc Bonferroni test was used for comparing intragroups and Tukey test for comparing intergroups. Results The mean value after demineralization is 245, 246, 250, and 247 μm for Groups I to IV. After remineralization, group I > group II > group III > group IV. After acid challenge for a period of 14 days, group I > group II > group III > group IV. Conclusion The resin infiltrant showed higher caries inhibition potential and superior acid resistance than CPP-ACP and nano-HA. Clinical significance The inhibition of caries progression by resin infiltration technique should be considered as an alternative approach to the more invasive therapies and warrants a place in the range of minimally invasive dentistry techniques. How to cite this article Arvindkumar A, Maheswari U, Kingston C, Gnanaseelan R, Jonathan Emil Sam R. Caries-preventive Efficacy of Resin Infiltrant, Casein Phosphopeptide-amorphous Calcium Phosphate, and Nanohydroxyapatite using Confocal Scanning Electron Microscope: An in vitro Study. J Oper Dent Endod 2017;2(1):6-10.

Application of Transmission Electron Microscope and Scanning Electron Microscope in experimental tumor studies

1990 ◽  
Vol 48 (3) ◽  
pp. 306-307
Author(s):  
Gao Fengming
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Malignant Transformation ◽  
Transmission Electron Microscope ◽  
Experimental Tumor ◽  
Transmission Electron ◽  
Embryo Cells ◽  
Tumor Studies ◽  
Scanning Electron

Transmission electron microscope(TEM) and scanning electron microscope(SEM) were widely used in experimental tumor studies. They are useful for evaluation of cellular transformation in vitro, classification of histological types of tumors and treating effect of tumors. We have obtained some results as follows:1. Studies on the malignant transformation of mammalian cells in vitro. Syrian golden hamster embryo cells(SGHEC) were transformed in vitro by ThO2 and/or ore dust. In a few days after dust added into medium, some dust crystals were phagocytized. Two weeks later, malignant transformation took place. These cells were of different size, nuclear pleomorphism, numerous ribosomes, increasing of microvilli on cell surface with various length and thickness, and blebs and ruffles(Figs. 1,2). Myelomonocytic leukemic transformation of mouse embryo cells(MEC) was induced in vitro by 3H-TdR. Transformed cells were become round from fusiform. The number of mitochondria and endoplasmic reticulum was reduced, ribosomes and nucleoli increased, shape of nuclei irregular, microvilli increased, and blebs and ruffles appeared(Fig. 3).

Ionic regulation and buoyancy in some planktonic organisms

1993 ◽  
Vol 73 (1) ◽  
pp. 15-23 ◽  
Author(s):  
C. Newton ◽  
W. T. W. Potts
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Body ◽  
Coelomic Fluid ◽  
Ionic Regulation ◽  
High Concentration ◽  
Emission Analysis ◽  
X Ray ◽  
Scanning Electron ◽  
Gastrovascular System

Magnesium/chlorine and sulphur/chlorine ratios have been measured in the body fluids of some planktonic organisms by x-ray emission analysis of frozen hydrated specimens in a scanning electron microscope. Homarus vulgaris (Anthropoda: Decapoda) larvae excluded Mg2+ and SO42-ions from the haemolymph, but to a lesser extent than does the adult lobster. Bipinnaria larvae of Asterias (Echinodermata) excluded Mg2+ and SO42-ions from the coelomic fluid. Obelia medusae excluded Mg2+ and SO42-ions from the mesogloea but concentrate them in the gastrovascular system. The high concentration of sulphate in the gastrovascular fluid of medusae has been confirmed by rhodizonate titration in Cyanea and Rhizostoma jellyfish. Some implications of magnesium and sulphate regulation are discussed.

Comparative evaluation of surface roughness and texture of polished nanofill and nanohybrid composites using profilometer and scanning electron microscope: in - vitro study

2017 ◽  
Vol 9 (2) ◽  
pp. 4-10
Author(s):  
Krishna Prasada L ◽  
Jyothsna S Jathanna ◽  
Naveen Kumar ◽  
Ramya M.K ◽  
Elizabeth Issac
Keyword(s):  
Surface Roughness ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Resin Composite ◽  
In Vitro Study ◽  
Average Value ◽  
Three Samples ◽  
Group B ◽  
Scanning Electron

BACKGROUND: To determine the effectiveness of three polishing systems on three different composite materials by evaluating surface roughness using a Profilometer and Scanning electron microscope. METHODOLOGY: A total of Sixty-three resin composite disks were prepared in rectangular acrylic mould of 8×2mm dimension, with 4mm thickness. Specimens were made of light activated resin composite Filtek Z-250-XT, Tetric-N-Ceram bulkfill, Ceram X Duo. The sixty-three samples were divided into three groups of twenty-one samples each i.e. Group A (Filtek Z-250-XT), Group B (TetricN-Ceram bulkfill) and Group C (Ceram-X-Duo).Out of 21 samples of each of the material, 7 specimens were polished with multi enhance polishing agents, 7 specimens were polished with super snap polishing agents and 7 specimens were polished with sof-lexdiscs.Surface roughness of each sample after polishingwasevaluatedusingProfilometer and Scanning electron microscope. RESULTS: One-way anova and kruskalwallis test was used for statistical analysis. Ceram-X-Duo gave least roughness average value with Super snap polishing system when compared to multi enhance and sof-lex polishing system. CONCLUSION: Super snap polishing system is a better polishing system than multi enhance and soflex and Ceram-X-Duo composite material offers better polishability compared to Filtek Z-250-XT and Tetric-N-Ceram bulk fill.

Sign in / Sign up

Export Citation Format

Share Document