A low prime extracorporeal system for small patients: a primate model

Perfusion ◽  
1987 ◽  
Vol 2 (1) ◽  
pp. 35-38
Author(s):  
D. Wheeldon ◽  
D. Bethune
Keyword(s):  
Rhesus Monkey ◽  
Lung Transplantation ◽  
Negative Pressure ◽  
Venous Drainage ◽  
Donor Blood ◽  
Primate Model ◽  
Monkey Model ◽  
Priming Volume ◽  
Bubble Oxygenator

In the course of developing a technique for heart and lung transplantation a 6 kg Rhesus monkey model was used. We required a low prime circuit, because it was difficult to obtain donor blood for these animals, which was also capable of supporting the circulation for up to two hours. We describe a system incorporating a semidisposable bubble oxygenator, run under controlled negative pressure with very short blood lines. This arrangement allows for a priming volume of 110 ml with good venous drainage control. The principle could be exploited for use with paediatric extracorporeal systems.

An Automatically Regulated Low Prime Heart-Lung Machine for Infants: A Rabbit Model

Perfusion ◽  
1987 ◽  
Vol 2 (2) ◽  
pp. 109-113
Author(s):  
Erik Wabeke ◽  
Piet H Mook ◽  
Jan M Elstrodt ◽  
Charles RH Wildevuur
Keyword(s):  
Cardiopulmonary Bypass ◽  
Negative Pressure ◽  
Rabbit Model ◽  
Gravity Drainage ◽  
Extracorporeal Circuit ◽  
Donor Blood ◽  
Minimal Volume ◽  
Priming Volume ◽  
Heart Lung Machine ◽  
Lung Machine

A new compact heart-lung machine for paediatric use was designed. The total volume of this system of only 90ml allows for priming without the use of donor blood. The priming volume could be kept small mainly by replacing gravity drainage with drainage by a negative pressure in the venous reservoir. To avoid volume shifts between the extracorporeal circuit and the infant's circulation and to safely operate this minimal volume circuit, the heart-lung machine was automatically controlled. In this study we show that the miniaturized system functioned reliably under various conditions during cardiopulmonary bypass in rabbits.

scholarly journals Optimal allogeneic islet dose for transplantation in insulin-dependent diabetic Macaca fascicularis monkeys

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Geun Soo Kim ◽  
Chan Woo Cho ◽  
Jong Hyun Lee ◽  
Du Yeon Shin ◽  
Han Sin Lee ◽  
...  
Keyword(s):  
Insulin Requirement ◽  
Exogenous Insulin ◽  
Islet Amyloid ◽  
Primate Model ◽  
Control Blood Glucose ◽  
T Cell Infiltration ◽  
Monkey Model ◽  
High Insulin ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

AbstractMany groups are working to improve the results of clinical allogeneic islet transplantation in a primate model. However, few studies have focused on the optimal islet dose for achieving normal glycemia without exogenous insulin after transplantation in primate models or on the relationship between rejection and islet amyloid polypeptide (IAPP) expression. We evaluated the dose (10,000, 20,000, and > 25,000 islet equivalents (IEQ)/kg) needed to achieve normal glycemia without exogenous insulin after transplantation using eleven cynomolgus monkeys, and we analyzed the characteristics exhibited in the islets after transplantation. 10,000 IEQ/kg (N = 2) failed to control blood glucose level, despite injection with the highest dose of exogenous insulin, and 20,000 IEQ/kg group (N = 5) achieved unstable control, with a high insulin requirement. However, 25,000 IEQ/kg (N = 4) achieved normal glycemia without exogenous insulin and maintained it for more than 60 days. Immunohistochemistry results from staining islets found in liver biopsies indicated that as the number of transplanted islets decreased, the amount of IAPP accumulation within the islets increased, which accelerated CD3+ T cell infiltration. In conclusion, the optimal transplantation dose for achieving a normal glycemia without exogenous insulin in our cynomolgus monkey model was > 25,000 IEQ/kg, and the accumulation of IAPP early after transplantation, which depends on the transplanted islet dose, can be considered one factor in rejection.

Far-field microwave dosimetry in a rhesus monkey model

1980 ◽  
Vol 1 (2) ◽  
pp. 149-160 ◽  
Author(s):  
Richard G. Olsen ◽  
Toby A. Griner ◽  
George D. Prettyman
Keyword(s):  
Rhesus Monkey ◽  
Far Field ◽  
Monkey Model

Establishment of a rhesus monkey model of chronic temporal lobe epilepsy using repetitive unilateral intra-amygdala kainic acid injections

2017 ◽  
Vol 134 ◽  
pp. 273-282 ◽  
Author(s):  
Yajie Chi ◽  
Bolin Wu ◽  
Jianwei Guan ◽  
Kuntai Xiao ◽  
Ziming Lu ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Temporal Lobe Epilepsy ◽  
Kainic Acid ◽  
Temporal Lobe ◽  
Monkey Model

Comparison of FD-TD and Experimentally Determined Local SAR Values in a Rhesus Monkey Model

1999 ◽  
pp. 287-290
Author(s):  
J. M. Ziriax ◽  
C. M. Furse ◽  
J. A. D’Andrea ◽  
J.-H. Gao ◽  
P. A. Mason ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Monkey Model ◽  
Local Sar

A multiparametric study of the action of mifepristone used in emergency contraception using the Rhesus monkey as a primate model

Contraception ◽  
2003 ◽  
Vol 68 (6) ◽  
pp. 453-469 ◽  
Author(s):  
Jayasree Sengupta ◽  
Latika Dhawan ◽  
P.G.L Lalitkumar ◽  
D Ghosh
Keyword(s):  
Rhesus Monkey ◽  
Emergency Contraception ◽  
Primate Model

scholarly journals Aggregatibacter actinomycetemcomitans colonization and persistence in a primate model

2019 ◽  
Vol 116 (44) ◽  
pp. 22307-22313 ◽  
Author(s):  
Senthil Kumar Velusamy ◽  
Vandana Sampathkumar ◽  
Narayanan Ramasubbu ◽  
Bruce J. Paster ◽  
Daniel H. Fine
Keyword(s):  
Aggregatibacter Actinomycetemcomitans ◽  
Aggressive Periodontitis ◽  
Microbial Interactions ◽  
Primate Model ◽  
Monkey Model ◽  
Oral Environment ◽  
Indigenous Microbiota ◽  
Weighted Network Analysis

Aggregatibacter actinomycetemcomitans is associated with aggressive periodontitis resulting in premature tooth loss in adolescents. Tooth adherence and biofilm persistence are prerequisites for survival in the oral domain. Here, using a rhesus monkey model, 16S rRNA sequencing, and weighted network analysis, we assessed colonization of A. actinomycetemcomitans variants and ascertained microbial interactions in biofilm communities. Variants in A. actinomycetemcomitans leukotoxin (ltx) were created, labeled, inoculated, and compared with their progenitor strain for in vivo colonization. Samples of tooth-related plaque were assessed for colonization at baseline and after debridement and inoculation of labeled strains. Null, minimal, and hyper-Ltx–producing strains were created and assessed for hydroxyapatite binding and biofilm formation in vitro. Ltx-hyperproducing strains colonized with greater prevalence and at higher levels than wild type or ltx mutants (P = 0.05). Indigenous and inoculated A. actinomycetemcomitans strains that attached were associated with lactate-producing species (i.e., Leptotrichia, Abiotrophia, and Streptoccocci). A. actinomycetemcomitans was found at 0.13% of the total flora at baseline and at 0.05% 4 wk after inoculation. In vivo data were supported by in vitro results. We conclude that hyper-Ltx production affords these strains with an attachment advantage providing a foothold for competition with members of the indigenous microbiota. Increased attachment can be linked to ltx gene expression and up-regulation of adherence-associated genes. Growth of attached A. actinomycetemcomitans in vivo was enhanced by lactate availability due to consorting species. These associations provide A. actinomycetemcomitans with the constituents required for its colonization and survival in the complex and competitive oral environment.

scholarly journals Fetal Surgery in the Primate 4.0: A New Technique 30 Years Later

2020 ◽  
pp. 1-7
Author(s):  
Erin E. Perrone ◽  
Laura A. Galganski ◽  
Alice F. Tarantal ◽  
Katie J. Olstad ◽  
Marjorie C. Treadwell ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Membrane Separation ◽  
Fetal Surgery ◽  
Standard Of Care ◽  
Gravid Uterus ◽  
Monkey Model ◽  
Near Term ◽  
A New Technique ◽  
Harmonic Ace ◽  
Fluid Levels

<b><i>Introduction:</i></b> Open fetal surgery requires a hemostatic hysterotomy that minimizes membrane separation. For over 30 years, the standard of care for hysterotomy in the gravid uterus has been the AutoSuture Premium Poly CS*-57 stapler. <b><i>Objective:</i></b> In this study, we sought to test the feasibility of hysterotomy in a rhesus monkey model with the Harmonic ACE®+7 Shears. <b><i>Methods:</i></b> A gravid rhesus monkey underwent midgestation hysterotomy at approximately 90 days of gestation (2nd trimester; term = 165 ± 10 days) using the Harmonic ACE®+7 Shears. A two-layer uterine closure was completed and the dam was monitored by ultrasound intermittently throughout the pregnancy. At 58 days after hysterotomy (near term), a final surgery was performed to evaluate the uterus and hysterotomy site. <b><i>Results:</i></b> A 3.5-cm hysterotomy was completed in 2 min 7 s. The opening was hemostatic and the membranes were sealed. Immediately after closure and throughout the pregnancy, ultrasound revealed intact membranes without separation and normal amniotic fluid levels. At term, the scar was well healed without signs of thinning or dehiscence. <b><i>Conclusions:</i></b> The Harmonic ACE®+7 Shears produced a hemostatic midgestation hysterotomy with membrane sealing in the rhesus monkey model. Importantly, healing was acceptable.

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