Cytotoxicity of Subpopulations of Splenic Cells from Normal and Fibrosarcoma - Bearing Mice towards Syngeneic Tumour Cells

1983 ◽  
Vol 69 (6) ◽  
pp. 489-496 ◽  
Author(s):  
Vimal Suhas Aroskar ◽  
Chintamani Shankar Godbole ◽  
Kamlakar Anant Chaubal
Keyword(s):  
Density Gradient ◽  
Cell Populations ◽  
Tumour Bearing ◽  
Splenic Cell ◽  
Cytotoxicity Studies ◽  
Tumour Bearing Mouse ◽  
Splenic Cells ◽  
Step Density ◽  
Group Density

The nonadherent splenic cells from normal and tumour-bearing (mouse fibrosarcoma-MFS) Swiss mice were divided into 6 subpopulations on Percoll step density gradient and characterised. For the determination of their cytotoxicity towards syngeneic MFS cells and their electrophoretic mobility (EPM), the splenic cell populations were pooled to form 2 broad groups: a lower-density group (density of saline to just < 1.069 g/ml) and a higher-density group (1.069 to just < 1.087 gm/ml). In general, the splenic cells from mice bearing 10- to 11-day-old MFS tumours differed in certain characteristics from those of normal mice in that they showed an increase in the following: proliferation, heterogeneity, with appearance of large cells (>70 μ2); cells with a lower density (< 1.069 g/ml); cells with a lower (< 0.85 μ/sec/Volt/cm) anodi cEPM. The cytotoxicity studies revealed that: a) the lower-density splenic cells of both normal and tumour-bearing mice were more cytotoxic than the higher-density splenic cells; b) the lower- and higher-density splenic cells of tumour-bearing mice were more cytotoxic than the corresponding cells of normal mice. These findings indicate that the splenic cells of mice with a lower EPM and a lower density are the main contributors of cell-mediated cytolysis of a subpopulation of MFS cells.

scholarly journals 5'-Nucleotidase in different populations of mouse lymphocytes.

1977 ◽  
Vol 25 (2) ◽  
pp. 97-103 ◽  
Author(s):  
R J Uusitalo ◽  
M J Karnovsky
Keyword(s):  
T Cells ◽  
B Cells ◽  
Cell Populations ◽  
Consistent Difference ◽  
Splenic Lymphocytes ◽  
Splenic Cells ◽  
Ultrastructural Radioautography ◽  
Different Populations ◽  
Immature Cells ◽  
Mouse Lymphocytes

The activity of 5'-nucleotidase in different populations of intact lymphocytes was studied using biochemical, cytochemical and radioautographic methods. In some strains of mice the results showed a consistent difference in 5'-nucleotidase (AMPase) content between intact thymic and splenic lymphocytes. In the R III, C 57, BALB/c, CBA and Cd-1 strains AMPase activity in the isolated splenic cells was foru to 10 times the activity of intact thymocytes. In highly enriched populations of splenic T and B cells the average AMPase activity was about the same. From separate assays it was seen that the AMPase activity in highly enriched populations of lymphoctes was variable so that within one experiment the T cells seemed to have the higher AMPase activity while in other experiments B cells shown to be more active than T cells. Ultrastructural radioautography was done to count AMPase positive cells within T and B cell populations, the latter identified b binding of I125-labelled anti-immunoglobulin. It was seen that about 50% of B cells, but only about 10% of T cells, were positive for AMPase. It is suggested that there is a subpopulation within B and T cell populations with a high membrane AMPase activity and another subpopulation with less or no enzyme activity. It is also suggested that the activity and/or the proportion of these positive cells is changing within the splenic cell population. By using cortisone to deplete the immature cells from the thymus it was seen that the remaining mature cells have about the same AMPase activity as did the immaturecells, and thus mature T cells must gain their high acitivity after leaving the thymus. By incubating splenic lymphocytes with Concanavalin A it was also seen that the immature transformed cells had the same amount of enzyme as did untransformed cells.

scholarly journals Synthesis, antimicrobial activity, and determination of the lipophilicity of ((cyclohex-3-enylmethylene)hydrazinyl)thiazole derivatives

2019 ◽  
Vol 28 (11) ◽  
pp. 2023-2036 ◽  
Author(s):  
Anna Biernasiuk ◽  
Magdalena Kawczyńska ◽  
Anna Berecka-Rycerz ◽  
Beata Rosada ◽  
Anna Gumieniczek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Positive Control ◽  
Reversed Phase ◽  
Thiazole Derivatives ◽  
Strong Activity ◽  
Cytotoxicity Studies ◽  
L929 Fibroblast ◽  
Layer Chromatography

Abstract Synthesis and investigation of antimicrobial activity of fifteen novel thiazoles containing cyclohexene moiety are presented. Among the derivatives, compounds 3a–3d, 3f, 3n, and 3o showed very strong activity against the reference Candida spp. strains with MIC = 0.015–3.91 µg/ml. The activity of these compounds is similar and even higher than the activity of nystatin used as positive control. Compounds 3d, 3f, 3n, 3o showed the highest activity with very strong effect towards most of yeasts isolated from clinical materials with MIC = 0.015–7.81 µg/ml. The cytotoxicity studies for the most active compounds showed that Candida spp. growth was inhibited at noncytotoxic concentrations for the mammalian L929 fibroblast. In addition, a good correlation was obtained between lipophilicity of compounds determined using reversed phase thin-layer chromatography and their antifungal activity.

Frontogenesis in a fluid with horizontal density gradients

1989 ◽  
Vol 202 ◽  
pp. 1-16 ◽  
Author(s):  
J. E. Simpson ◽  
P. F. Linden
Keyword(s):  
Density Distribution ◽  
Density Gradient ◽  
Initial Density ◽  
Vertical Shear ◽  
Horizontal Gradient ◽  
Constant Density ◽  
Piecewise Constant ◽  
Vertical Density ◽  
Step Density ◽  
Horizontal Density Gradient

The adjustment under gravity of a fluid containing a horizontal density gradient is described.’ The fluid is initially at rest and the resulting motion is calculated as the flow accelerates, driven by the baroclinic density field. Two forms of the initial density distribution are considered. In the first the initial horizontal gradient is constant. A purely horizontal motion develops as the isopycnals rotate towards the horizontal. The vertical density gradient increases continually with time but the horizontal density gradient remains unchanged. The horizontal velocity has a uniform vertical shear, and the gradient Richardson number is constant in space and decreases monotonically with time to ½. The second density distribution consists of a piecewise constant gradient with a jump in the gradient along a vertical isopycnal. The density is continuous. In this case frontogenesis is predicted to occur on the isopycnal between the two constant-density-gradient regions, and the timescale for the formation of a front is determined. Laboratory experiments are reported which confirm the results of these calculations. In addition, lock exchange experiments have been carried out in which the horizontal mean gradient is represented by a series of step density differences separated by vertical gates.

Sign in / Sign up

Export Citation Format

Share Document