scholarly journals Pathogenesis of Renal Lesions in Chickens After Experimental Infection With 9a5b Newcastle Disease Virus Mutant Isolate

2016 ◽  
Vol 54 (1) ◽  
pp. 94-98 ◽  
Author(s):  
A. El-Bahrawy ◽  
A. Zaid ◽  
Y. Sunden ◽  
M. Sakurai ◽  
H. Ito ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Kidney Tissue ◽  
Rt Pcr ◽  
Tissue Samples ◽  
Infectious Bronchitis ◽  
Avian Nephritis Virus ◽  
Specific Pathogen ◽  
Virus Mutant

In this study, we investigated the pathogenesis of Newcastle disease virus (NDV) in the chicken kidney. Twenty-six 32-day-old specific pathogen-free chickens were intranasally inoculated with the 9a5b NDV mutant isolate. Kidney tissue samples, collected at 6 and 12 hours postinoculation (hpi) and 1, 2, 3, 5, and 10 days postinoculation (dpi), were analyzed by histopathology, immunohistochemistry (IHC), reverse transcription polymerase chain reaction (RT-PCR), and virus titration. Histopathologically, tubulointerstitial nephritis was detected in the renal cortex and predominantly in the medulla. Nephrotropism of 9a5b NDV was confirmed by IHC, RT-PCR, and virus isolation. Massive degenerative changes and infiltration of CD3-immunopositive cells accompanied replication of the 9a5b NDV isolate in chicken kidneys. In conclusion, pathological changes that were caused by NDV in chicken kidneys were similar to those caused by avian influenza virus, infectious bronchitis virus, and avian nephritis virus, and this highlights the importance of including NDV in the differential diagnosis of kidney disease in chickens.

scholarly journals Molecular characterisation of Newcastle disease virus exotic isolate in East Java, Indonesia

2021 ◽  
Vol 24 (2) ◽  
pp. 191-199
Author(s):  
N. P. Kusumarahayu ◽  
N. Putri ◽  
R. Ernawati ◽  
J. Rahmahani ◽  
S. Suwarno ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Rt Pcr ◽  
Genetic Characteristics ◽  
Basic Work ◽  
Specific Pathogen ◽  
Baseline Information ◽  
Native Chickens

Newcastle disease virus (NDV) is ssRNA paramyxovirus causing clinical signs, varying from subclinical infections to 100% mortality in infected chickens. Haemagglutinin-neuraminidase (HN) protein has an important role related to infection and pathogenesis, therefore, the protein was characterised in this study. Samples were collected from 45 cloacal swabs of native chickens. They were isolated by inoculating in specific pathogen-free embryonated eggs. Molecular detection of NDV was done by reverse transcriptase polymerase chain reaction (RT-PCR) encoding HN protein. RT-PCR for HN gene of NDV generated DNA fragments sized 503 bp, which were then sequenced using ABI Prism. The results have shown that virus isolates were mostly lentogenic and might contribute to outbreak in East Java, Indonesia. Based on this fact, NDV infected native chickens can act as reservoir and contribute to outbreak in the poultry. Our study provides baseline information on genetic characteristics of NDV circulating in East Java and serves as a basic work for further research.

scholarly journals Construction and Immunogenicity of Novel Chimeric Virus-Like Particles Bearing Antigens of Infectious Bronchitis Virus and Newcastle Disease Virus

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 254 ◽  
Author(s):  
Xuan Wu ◽  
Xiwen Zhai ◽  
Yan Lai ◽  
Lei Zuo ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Infectious Bronchitis Virus ◽  
Newcastle Disease ◽  
Vaccine Platform ◽  
Virus Like Particles ◽  
Infectious Bronchitis ◽  
Specific Pathogen ◽  
Virulent Challenge ◽  
Carboxy Terminal

Infectious bronchitis virus (IBV) and Newcastle disease virus (NDV) are two poultry pathogens seriously affecting the poultry industry. Here, IBV S1 and the ectodomain of NDV F proteins were separately linked with the trans-membrane and carboxy-terminal domain of IBV S protein (STMCT), composing rS and rF; thus, a novel chimeric infectious bronchitis-Newcastle disease (IB-ND) virus-like particles (VLPs) vaccine containing the rS, rF, and IBV M protein was constructed. Under the transmission electron microscope (TEM), VLPs possessing similar morphology to natural IBV were observed. To evaluate the immunogenicity of chimeric IB-ND VLPs, specific pathogen-free (SPF) chickens were immunized with three increasing doses (50, 75, and 100 μg protein of VLPs). Results of ELISAs detecting IBV and NDV specific antibodies and IL-4 and IFN-γ T cell cytokines indicated that vaccination with chimeric IB-ND VLPs could efficiently induce humoral and cellular immune responses. In the challenge study, chimeric IB-ND VLPs (100 μg protein) provided 100% protection against IBV or NDV virulent challenge from death, and viral RNA levels in tissues and swabs were greatly reduced. Collectively, chimeric IB-ND VLPs are highly immunogenic and could provide complete protection from an IBV or NDV virulent challenge. Chimeric IB-ND VLPs are an appealing vaccine candidate and a promising vaccine platform bearing multivalent antigens.

scholarly journals STUDY THE EFFECT OF MYCOPLASMA CONTAMINATION OF EGGS USED IN VIRUS TITRATION AND EFFICACY OF SOME LIVE ATTENUATED POULTRY VIRAL VACCINES

2016 ◽  
Vol 10 (1) ◽  
pp. 216 ◽  
Author(s):  
Marwa Fathy ◽  
Mounir M. El-safty ◽  
Jakeen K. El-jakee ◽  
Howaida I. Abd-alla ◽  
Hala Mahmoud
Keyword(s):  
Disease Virus ◽  
Newcastle Disease ◽  
Disease Process ◽  
Mycoplasma Gallisepticum ◽  
Infectious Bursal Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infectious Bronchitis ◽  
Bursal Disease ◽  
Specific Pathogen ◽  
Viral Vaccines

ABSTRACTObjective: The study of Mycoplasma gallisepticum (MG) infection is needed, not only to understand the disease process but also to understand theinterference with the evaluation of some live viral poultry vaccines. This study aims to investigate the titration and potency of some live attenuatedpoultry viral vaccines; Newcastle disease, infectious bronchitis, infectious bursal disease, and Reo in both specific pathogen-free (SPF) embryonatedchicken eggs (ECEs) and chickens.Methods: Titration of live attenuated viral poultry vaccines in ECEs was carried out by dividing the inoculated eggs into four groups; the pre-,simultaneously-, post-, and non-MG contaminated. MG effect on the potency test was carried out using seventeen groups of SPF chickens (25 chicken/group) placed into separate isolators. Each live attenuated viral poultry vaccine was inoculated into 4 groups.Results: The highest titer of these vaccines that appeared in MG pre- contaminated ECEs were 1011, 107.5, 107.9, and 10, respectively. The lowest vaccinetiters that appeared in non-MG contaminated ECEs were 108, 106, 106.8, and 1067.5, respectively. Although the potency of these previous vaccines indicated thatthe highest antibodies titer that appeared in MG pre-infected vaccinated chickens were 7.5 log, 36 enzyme-linked immunosorbent assay unit (EU), and42 EU, respectively; the lowest antibodies titer that appeared in non-MG infected vaccinated chickens were 6.5 log22, 12 EU, 17 EU, and 10 EU, respectively.Conclusion: The present study findings underline the importance of using Mycoplasma -free eggs or chicken for the production of virus vaccines.Keywords: Mycoplasma gallisepticum, Newcastle disease virus, Infectious bronchitis virus, Infectious bursal disease virus, Reo virus, Chicken, Specificpathogen-free eggs.

Isolation and Molecular Characterization of Newcastle Disease Virus in Layers

2021 ◽  
Author(s):  
Smita Bordoloi ◽  
Anju Nayak ◽  
A.P. Singh ◽  
R.V. Singh ◽  
Kajal Jadav ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virulent Strain ◽  
Indian Subcontinent ◽  
Economic Losses ◽  
Tissue Samples ◽  
F Gene ◽  
Virulent Newcastle Disease Virus

Background: Newcastle disease (ND) in spite of the availability of vaccines remains a constant threat to poultry producers worldwide. It is prevalent in Indian subcontinent and leads to economic losses. The present study was aimed with isolate and identify virulent Newcastle disease virus (NDV) in layer poultry from field outbreaks.Methods: Total 47 samples consisting of nasal (05), oropharyngeal (13) and cloacal swabs (11) and tissue samples consisting of trachea (07), lungs (06), larynx (05) were collected from layer birds. For isolation of NDV swab and tissue samples were inoculated in 9-11 days old embryonated eggs via allantoic cavity route. After preparing the viral inoculum, 47 suspected samples (29 swab and 18 tissue samples) were inoculated in 141 embryonated eggs to isolate the virus.Result: Out of 47 samples 10 (21.27%) samples were positive for HA activity. All the 10 isolates showing HA activity subjected to Reverse-Transcriptase PCR of F gene and 6 were found positive in RT-PCR for F1 gene. The PCR amplified product showed amplicon at 356 bp and 254 bp positive for F1 and F2 gene, respectively. On basis of F gene, 06 (50%) isolates were considered as virulent Newcastle Disease Virus. One isolate sequence was submitted at NCBI with accession MT890653 On phylogenetic analysis MT890653 designated as Class II/ genotype II/ virulent strain and had the motif 112R-R-R-K-R-F117 at the cleavage site of the fusion protein.

Evaluation of RT-PCR for Rapid Detection of Sudanese Isolates and Vaccine Strains of Newcastle Disease Virus

2005 ◽  
Vol 8 (3) ◽  
pp. 418-420
Author(s):  
Mohamed A.M. Yousof . ◽  
I.E. Aradaib . ◽  
K.M.S. Khairalla . ◽  
M.A. Abdalla . ◽  
A.R.E. Karrar . ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Rapid Detection ◽  
Rt Pcr

scholarly journals First Genome Sequence of Newcastle Disease Virus of Genotype VIIi from Jordan

2018 ◽  
Vol 7 (23) ◽  
Author(s):  
Mustafa Ababneh ◽  
Helena L. Ferreira ◽  
Mohammad Khalifeh ◽  
David L. Suarez ◽  
Claudio L. Afonso
Keyword(s):  
Reverse Transcriptase ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Illumina Sequencing ◽  
Newcastle Disease ◽  
Rt Pcr ◽  
Total Rna ◽  
Reverse Transcriptase Pcr ◽  
Chicken Spleen

Newcastle disease virus (NDV) was detected by reverse transcriptase PCR (RT-PCR) from total RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete coding genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained with MiSeq (Illumina) sequencing.

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