Melanosis Coli in Pigs Coincides With High Sulfate Content in Drinking Water

Melanosis coli is a well-described condition in humans, characterized by the accumulation of lipofuscin-laden macrophages in the lamina propria of the colon, giving it a dark tone. An increased apoptosis rate of colonic epithelial cells appears to be the underlying pathogenesis. In pigs, oxidative damage has been proposed as one of the causes for melanosis coli. In this article, we report a series of cases of melanosis coli in pigs affecting several finishing units in the south of Spain. Large intestines had dark green to brown pigmentation of the mucosa. Histological, histochemical, and ultrastructural studies confirmed a high number of lipofuscin-laden macrophages in the lamina propria of the rectum and colon, which additionally stained positive for the apoptosis marker cleaved caspase-3. Of note, all affected finishing units utilized water supply with a high content of sulfates, which may be one of the causes for melanosis coli development in pigs.

Download Full-text

Improved electroimmunoassay of factor VIII-related antigen.

Clinical Chemistry ◽

10.1093/clinchem/23.3.602 ◽

1977 ◽

Vol 23 (3) ◽

pp. 602-603 ◽

Cited By ~ 4

Author(s):

A Chute ◽

J E Haddow ◽

R F Ritchie

Keyword(s):

Factor Viii ◽

Major Part ◽

Sulfate Content ◽

Factor Viii Related Antigen ◽

Factor Viii Antigen ◽

High Sulfate

Abstract Factor VIII-related antigen migrates poorly into gel during electrophoresis in agarose, probably in major part because of the relatively high sulfate content of the commonly used agars. A recently available low-sulfate residue agar, substituted for standard agar preparations in electroimmunoassay, allows Factor VIII antigen to travel more rapidly, producing "rockets" that are well defined and easily measured, making the assay easier and more reliable.

Download Full-text

Aluminophosphate Glasses with High Sulfate Content

MRS Proceedings ◽

10.1557/proc-353-101 ◽

1994 ◽

Vol 353 ◽

Cited By ~ 9

Author(s):

Sergey V. Stefanovsky ◽

Igor A. Ivanov ◽

Anatolii N. Gulin

Keyword(s):

Diffusion Coefficients ◽

Glass Structure ◽

Chemical Durability ◽

Electric Resistivity ◽

Melting Points ◽

Sulfate Content ◽

Leach Rate ◽

High Sulfate ◽

Aluminophosphate Glasses ◽

And Diffusion

AbstractTo immobilize a high sulfate radioactive wastes a system Na2O-A12O3-P2O5-SO3 has been chosen as one where glasses have a relatively low melting points and good chemical durability. Glasses within partial system 44 Na2O, 20 A12O3 (36-x) P2O5 x SO3 have been prepared at 1000 °C. A possibility of assimilation up to 12 mole % of SO3 has been established. The basic properties of sulfate-containing glasses as density, microhardness, thermal expansion coefficient, transformation and deformation temperatures, viscosity, electric resistivity, leach rate of ions and diffusion coefficients of 22Na, 35S, 90Sr and 137Cs have been measured. Glass structure by infrared and EPR spectroscopies has been investigated.

Download Full-text

Characterization of Mellowing Process to Control Expansion in High-Sulfate-Bearing Soil

Transportation Research Record Journal of the Transportation Research Board ◽

10.1177/03611981211036345 ◽

2021 ◽

pp. 036119812110363

Author(s):

Chang-Seon Shon ◽

Tom Scullion ◽

Wade Blackmon ◽

Dichuan Zhang ◽

Jong Ryeol Kim

Keyword(s):

Fly Ash ◽

Sulfate Content ◽

Rapid Reduction ◽

3 Dimensional ◽

Volumetric Expansion ◽

Chemical Attack ◽

Laboratory Test Results ◽

Soluble Sulfate ◽

High Sulfate

Calcium-based stabilizing materials (CBSMs) such as lime and fly ash are extensively used in subgrade primarily to enhance mechanical strength and improve resistance to chemical attack, resulting in more durable roadway. The soluble sulfate phase contained in some soils, however, can react with CBSMs and form ettringite minerals. If the soil is compacted before the end of this reaction, large, unstable, and volumetric swelling can occur. Among several methods to control sulfate-induced swelling, a “mellowing” approach is typically used because of its efficient, economical, and practical benefits when dealing with calcium-based stabilization of soils with significant soluble sulfate contents. Although the mellowing method is one of the frequently used methods, little data is available on the characterization of the specified mellowing process in the high-sulfate-bearing soil during the mellowing period. A research program investigated key factors influencing the mellowing process during the mellowing period, explaining how stabilizer type and content, remixing interval, mellowing period, and temperature play a role in reducing soluble sulfate content. Moreover, for selected mixtures, the 3-dimensional volumetric expansion and retained strength were measured after the mellowing process. Laboratory test results have revealed that a single mellowing process with higher lime content and daily remixing at high temperature leads to the rapid reduction of sulfate content in the soil. Moreover, after the mellowing process, additional soil treatment with fly ash or a combination of lime and fly ash leads to lower expansion and higher retained unconfined compressive strength of the soil mixture.

Download Full-text

Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro

Carbohydrate Polymers ◽

10.1016/j.carbpol.2005.04.007 ◽

2005 ◽

Vol 61 (2) ◽

pp. 148-154 ◽

Cited By ~ 97

Author(s):

Ronge Xing ◽

Song Liu ◽

Huahua Yu ◽

Zhanyong Guo ◽

Zhien Li ◽

...

Keyword(s):

Molecular Weight ◽

Antioxidant Activity ◽

High Molecular Weight ◽

Sulfate Content ◽

High Sulfate

Download Full-text

Antihyperlipidemic activity of high sulfate content derivative of polysaccharide extracted from Ulva pertusa (Chlorophyta)

Carbohydrate Polymers ◽

10.1016/j.carbpol.2011.09.073 ◽

2012 ◽

Vol 87 (2) ◽

pp. 1637-1640 ◽

Cited By ~ 70

Author(s):

Huimin Qi ◽

Liye Huang ◽

Xiaolei Liu ◽

Dongmei Liu ◽

Quanbin Zhang ◽

...

Keyword(s):

Ulva Pertusa ◽

Sulfate Content ◽

Antihyperlipidemic Activity ◽

High Sulfate

Download Full-text

Antioxidant and antihyperlipidemic activities of high sulfate content purified polysaccharide from Ulva pertusa

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.11.061 ◽

2020 ◽

Vol 146 ◽

pp. 756-762 ◽

Cited By ~ 3

Author(s):

Bingxi Li ◽

Haiting Xu ◽

Xiaoqian Wang ◽

Yuzhou Wan ◽

Nanfang Jiang ◽

...

Keyword(s):

Ulva Pertusa ◽

Sulfate Content ◽

High Sulfate

Download Full-text

Ion Pairing and Water Quality Measurements

Canadian Journal of Earth Sciences ◽

10.1139/e71-048 ◽

1971 ◽

Vol 8 (4) ◽

pp. 468-476 ◽

Cited By ~ 20

Author(s):

T. M. L. Wigley

Keyword(s):

Water Quality ◽

Equilibrium Constants ◽

Ion Pairing ◽

Saturation Indices ◽

Sulfate Content ◽

Water Analyses ◽

High Sulfate ◽

Quality Measurements

The effect of ion pairing on water analyses is examined. Significant changes in composition details and saturation indices can occur in waters of moderate-to-high sulfate content. Revised estimates of the equilibrium constants for gypsum and dolomite for various temperatures are presented.

Download Full-text

TiO2 with a high sulfate content—thermogravimetric analysis, determination of acid sites by infrared spectroscopy and catalytic activity

Catalysis Today ◽

10.1016/s0920-5861(03)00195-0 ◽

2003 ◽

Vol 85 (1) ◽

pp. 69-74 ◽

Cited By ~ 42

Author(s):

Lúcia Kiyomi Noda ◽

Rusiene Monteiro de Almeida ◽

Norberto Sanches Gonçalves ◽

Luiz Fernando Dias Probst ◽

Oswaldo Sala

Keyword(s):

Catalytic Activity ◽

Infrared Spectroscopy ◽

Thermogravimetric Analysis ◽

Acid Sites ◽

Sulfate Content ◽

High Sulfate ◽

Analysis Determination

Download Full-text

P073 An increased autophagy and decreased apoptosis is detected in intestinal fibroblasts from Crohn’s Disease patients

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjab076.202 ◽

2021 ◽

Vol 15 (Supplement_1) ◽

pp. S176-S177

Author(s):

L Gisbert-Ferrandiz ◽

M Queralt ◽

J Cosín-Roger ◽

S Coll ◽

C Bauset ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Lamina Propria ◽

Caspase 3 ◽

Statistical Significance ◽

Intestinal Tissue ◽

Protein Ratio ◽

Protein Levels ◽

Mtor Protein ◽

Significant Increment

Abstract Background Fibrosis is a complication commonly present in Crohn’s disease (CD) patients with a structuring (B2) or penetrating (B3) phenotype, with no effective treatment. This process is characterized by a disequilibrium between the production and degradation of the extracellular matrix (ECM), mainly regulated by myofibroblasts. We aim to analyse here, the expression of markers of autophagy, apoptosis and proliferation in intestinal fibroblasts from CD patients. Methods Fibroblasts were isolated from the damaged intestinal mucosa of CD patients with a penetrating and stenotic behaviour. Control cells were obtained from the non-damaged intestine of patients with colorectal cancer. Protein levels of markers of autophagy and apoptosis were determined by Western Blot in isolated fibroblasts. The proliferation marker Ki67 was analysed by immunohistochemistry (IHC) in 5 µm slides of intestinal tissue from control or CD patients. Statistical significance was measured by t-test. Results In fibroblasts from CD patients, we detected a significant decrease in the ratio phospho-mTOR / mTOR (Fig. A) in parallel with a non-significant increment in the LC3 II / LC3 I protein ratio (174% ± 46.5), and a decrease in p62 protein levels (84.8% ± 5.5). When compared between CD behaviours, a significant decreased in the phospho-mTOR / mTOR protein ratio was detected in fibroblasts from B2- compared to that obtained in cells from B3-CD patients (Fig. B). The analysis of the expression of an apoptosis marker, Caspase 3, revealed a decreased of cleaved caspase 3 protein levels in CD fibroblasts compared to levels detected in control cells (Fig C). Finally, we observed in the lamina propria of the intestine from CD patients an increased number of Ki67 positive cells, compared to that detected in control tissue. Conclusion Our data show an increased autophagy and decreased apoptosis in isolated intestinal fibroblasts from CD patients; the high number of cells proliferating in the lamina propria of the intestinal tissue of these patients, strongly suggests a higher viability of these cells in the fibrotic context.

Download Full-text