Canine Nephrotoxic Glomerulonephritis: A Combined Light, Immunofluorescent, and Ultrastructural Study

1973 ◽  
Vol 10 (1) ◽  
pp. 69-86 ◽  
Author(s):  
N. G. Wright ◽  
H. Thompson ◽  
H. J. C. Cornwell
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Intravenous Administration ◽  
Ultrastructural Study ◽  
Complement C3 ◽  
Rabbit Serum ◽  
Mesangial Proliferation ◽  
Experimental Animals ◽  
Glomerular Capillary

The sequential pathology of canine nephrotoxic glomerulonephritis was studied by light microscopy, immunofluorescence and electron microscopy. The disease was induced by intravenous administration of antiglomerular basement membrane serum, and experimental animals were examined from 4 h up to 60 days after administration of nephrotoxic serum. During the first few days there was extensive glomerular capillary necrosis and infiltration by neutrophils. This was followed by mesangial proliferation and glomerular scarring. Using immunofluorescence, nephrotoxic (rabbit) serum and host IgG and complement (C3) were detected in damaged glomeruli.

scholarly journals THE GLOMERULUS IN EXPERIMENTAL RENAL DISEASE IN RATS AS OBSERVED BY LIGHT AND ELECTRON MICROSCOPY

1955 ◽  
Vol 102 (5) ◽  
pp. 573-580 ◽  
Author(s):  
Carolyn F. Piel ◽  
Luther Dong ◽  
F.W.S. Modern ◽  
Joseph R. Goodman ◽  
Roger Moore
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
Crescent Formation ◽  
Colloidal Iron ◽  
Narrow Channels ◽  
Glomerular Capillary

Nephrotoxic serum disease in rats has been studied by light and electron microscopy from 1 hour to 10 weeks after production of the disease. By light microscopy leucocytic infiltration of the glomerular capillary was observed between the 3rd and 6th hour. At 6 hours an increase in colloidal iron-positive material was observed coating the extraluminal surface of the capillaries. Also at this time swelling of the endothelial cells becomes prominent. By 72 hours, thickening of the basement membrane was observed. Glomerular capillary thrombi were observed in approximately half the tissue examined in the first 2 weeks of disease. 50 per cent of the animals showed severe chronic lesions, exudation into the capsular space, crescent formation, and obliteration of glomeruli. At 1 hour electron microscopic pictures showed that osmophilic material may line the foot processes of the epithelial cells and obliterate all but narrow channels of the space between the feet. By 6 hours thickening of the basement membrane was prominent. This change persisted throughout 10 weeks of observation. The tissue from animals which had severe chronic alterations by light microscopy revealed changes which could not be interpreted at this time.

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

Erythrophagocytosis by Entamoeba histolytica.- Ultrastructural Study

1972 ◽  
Vol 30 ◽  
pp. 156-157 ◽  
Author(s):  
Norberto Treviño ◽  
Alfredo Feria-Velasco ◽  
I. Ruiz de Chávez
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Entamoeba Histolytica ◽  
Ultrastructural Study ◽  
Saline Solution ◽  
Physiological Saline ◽  
Ultrastructural Level ◽  
Hot Plate ◽  
Physiological Saline Solution ◽  
Axenic Conditions

Although erythrophagocytosis by various species of Entamoeba is a well known phenomenon this has not yet been studied in detail at the ultrastructural level. The present work deals with the description of the incorporation process of erythrocytes by trophozoites of E. histolytica. For this study, trophozoites of E. histolytica, HK-9:NIH strain cultured in axenic conditions and washed human erythrocytes were placed on a hot plate at 37°C in physiological saline solution. After 5 minutes, 2.5% glutarldehyde was added and the samples were processed according to conventional techniques for electron microscopy.Based upon light microscopy studies on living trophozoites in contact with erythrocytes, it seems that erythrophagocytosis only takes place in one pole of the parasite.

scholarly journals ELECTRON MICROSCOPY OF THE AVIAN RENAL GLOMERULUS

1957 ◽  
Vol 3 (2) ◽  
pp. 183-192 ◽  
Author(s):  
R. K. F. Pak Poy ◽  
J. S. Robertson
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Thin Sheet ◽  
Cell Mass ◽  
Central Cell ◽  
Dense Layer ◽  
Central Mass ◽  
Capillary Basement Membrane ◽  
Glomerular Capillary ◽  
Glomerular Capillaries

Electron microscopy of sections of chicken glomeruli shows them to possess a large central cell mass, occupying the hilum and the centre of the glomerulus, and continuous with the adventitia of the afferent and efferent arterioles. The glomerular capillaries form a much simpler system than in mammals and are spread over the surface of the central cell mass. Between the capillaries the mass is limited externally by the major component of the glomerular capillary basement membrane, which continues over the surface of the mass from one capillary to the next. Projections of the central cell mass characteristically form the support for glomerular capillaries, and smaller knobs of the central mass may project actually into the lumen of the capillaries, but always carry a layer of endothelial cytoplasm before them. They are never in direct contact with blood. The basement membrane of the glomerular capillary loop has a central dense layer and two lateral less dense layers as in mammals. The central dense layer is continuous with similar appearing dense material in the intercellular spaces of the adventitiae of the arterioles, and also with that of the central cell mass. The two less dense layers can also be traced into direct continuity with the less dense regions of this intercellular substance. The endothelial cytoplasm is spread as a thin sheet over the inner surface of the capillary basement membrane, and shows scattered "pores" resembling those described in mammals. Epithelial cells with interlacing pedicels are at least as prominent as those in mammals. Bowman's capsular membrane also possesses three layers similar to but less wide than those of the capillary basement membrane, and all three layers can be traced into continuity with the dark and light regions of the intercellular material of the adventitial cells of the arterioles, and beyond them with that of the central cell mass. At the hilum Bowman's capsular membrane also fuses with the capillary basement membrane.

scholarly journals Effects of Chronic Organic Arsenic Exposure on Rats Kidney - Light Microscopy and Electron Microscopy Study

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Wan Muhamad Salahudin Wan Salleh ◽  
Norlelawati A. Talib ◽  
Nor Zamzila Abdullah ◽  
Sanda Aung ◽  
Zunariah Buyong
Keyword(s):  
Electron Microscopy ◽  
Treatment Group ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Arsenic Exposure ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Oral Gavage ◽  
Study Results ◽  
Organic Arsenic

Introduction:  Monosodium methylarsonate (MSMA) is an organic arsenical pesticide widely used in agriculture. Humans are exposed to arsenic through drinking water and anthropogenic activities. Exposure to inorganic arsenic has been linked with multiple health problems. However, studies focusing on chronic organic arsenic exposure and its adverse effects on kidney were limited. The purpose of current study was to determine the effects of chronic organic arsenic exposure in rats kidney by light and electron microscopy. Materials and Method:  Thirty-six male SpragueDawley rats were divided into six groups (n=6); three control and three treatment group respectively. All the control group was given distilled water via oral gavage. The treatment group was given oral gavage of MSMA at 42.10 mg/kg body weight (BW) which is equivalent 1/30 LD50 of MSMA. The control and treatment groups were sacrificed at two month, four month and six months interval. Both kidneys harvested for light microscopy and electron microscopy study. Results: Showed progressive changes. The changes initially focal and became diffused involving glomerular; such as glomerular hypercellularity, glomerular shrinkage and dilated Bowman's space. Meanwhile, in proximal tubules, showed diminished brush borders, detachment of nucleus and basement membrane thickening. Electron microscopy showed flattened cell bodies of podocytes, effacement and fusion of podocytes foot processes, thickening of glomerular basement membrane, and discontinuity of brush border. The control and two-months treated group appeared to be normal. Conclusion:  Chronic organic arsenic (MSMA) exposure induced chronic kidney injury.

Demonstration of pulmonary vascular perfusion by electron and light microscopy

1993 ◽  
Vol 75 (4) ◽  
pp. 1877-1883 ◽  
Author(s):  
M. F. Konig ◽  
J. M. Lucocq ◽  
E. R. Weibel
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Rabbit Serum ◽  
Capillary Perfusion ◽  
Vascular Perfusion ◽  
Thin Sections ◽  
Gold Particles ◽  
Capillary Recruitment

To estimate the fraction of dense pulmonary capillary network that is perfused under physiological conditions, we developed a new method for the demonstration of in vivo capillary perfusion by light and electron microscopy. Blood plasma was labeled by 8-nm colloidal gold particles coated with rabbit serum albumin. In anesthetized rabbits, 4#x2013;5 ml of this tracer were injected into the right atrium. Two and 15 min later, the circulation was interrupted by a snare around the heart, and the lung was fixed by instillation with glutaraldehyde. Gold particles were found in the plasma space of alveolar capillaries as well as in other organs. A random sample of thin sections studied by electron microscopy revealed that the entire capillary bed of the lung was perfused at least with plasma within 2 min after tracer infusion. Light microscopy of silver-enhanced sections showed areas with different staining intensities but no obviously unperfused capillaries. The concept of capillary recruitment, which would require a significant fraction of capillaries unperfused at rest, may have to be reassessed to consider time factors as well as the two-phase nature of blood; red blood cells and plasma may take different paths.

scholarly journals A Case of Proliferative Glomerulonephritis with Monoclonal IgG Deposits That Showed Predominantly Membranous Features

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
Homare Shimohata ◽  
Kentaro Ohgi ◽  
Hiroshi Maruyama ◽  
Yasunori Miyamoto ◽  
Mamiko Takayashu ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Proliferative Glomerulonephritis ◽  
Light Chains ◽  
The Novel ◽  
Immunofluorescence Study ◽  
Pathological Findings ◽  
Mesangial Proliferation ◽  
Glomerular Capillary ◽  
Almost All ◽  
Capillary Walls

In 2004, the novel category of monoclonal IgG deposition disease has been proposed and termed “proliferative glomerulonephritis with monoclonal IgG deposits” (PGNMID). This disease is characterized by membranoproliferative glomerulonephritis and staining for a single light-chain isotype and gamma heavy-chain subclass. A 76-year-old male who had monoclonal gammopathy was referred to our hospital because of proteinuria. The renal biopsy showed diffuse thickening of the glomerular capillary walls with focal mesangial proliferation. On immunofluorescence study, only IgG1 among the four subclasses and lambda light chains were detected mainly in the glomerular capillary walls. From these results, we diagnosed our case as PGNMID showing predominantly membranous features. Almost all pathological findings on light microscopy of PGNMID are membranoproliferative GN or endocapillary proliferative GN, while membranous GN cases are rare. Here, we present the case of PGNMID that showed predominantly membranous features on light microscopy.

scholarly journals Relationship of Proteinuria to Glomerular Basement Membrane Deposits in Serum-Sickness Glomerulonephritis in Rabbits

1977 ◽  
Vol 14 (5) ◽  
pp. 482-489 ◽  
Author(s):  
J. R. Easley ◽  
W. H. Halliwell
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Glomerular Basement Membrane ◽  
Serum Sickness ◽  
Ultrastructural Changes ◽  
Mesangial Matrix ◽  
Foot Process ◽  
Relationship Of

Fifteen rabbits were injected with one dose of 250 milligrams per kilogram of bovine serum albumin intravenously to induce acute serum sickness. The kidneys were removed from groups of five rabbits each at 10, 12 and 14 days after injection and examined by light, electron and immunofluorescent microscopy for evidence of immune complex glomerulonephritis. Urine was examined for protein when rabbits died. Glomerulonephritis was found in eight rabbits by light microscopy and in 10 rabbits by electron microscopy. Only one rabbit had positive immunofluorescence for immunologic components and only two rabbits had proteinuria. Hypercellularity was the primary change seen by light microscopy. Ultrastructural changes were numerous and various, and the major changes recognized were irregularity of the glomerular basement membrane, increased mesangial matrix, hypercellularity, endothelial hypertrophy, mesangial deposits, epithelial foot process fusion and mesangial circumferential interposition. Subepithelial humps were seen in only four rabbits; none of these rabbits had proteinuria. We concluded that increased permeability followed the deposition of immune complexes and development of ultrastructural lesions.

scholarly journals Microscopic study of edema in hydatidiform mole

2014 ◽  
Vol 14 (3) ◽  
pp. 261-268
Author(s):  
Olivar C. Castejón ◽  
Aury Caraballo ◽  
Oliver Castejón ◽  
Elizabeth Cedeño
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Hydatidiform Mole ◽  
Three Dimensional ◽  
Wide Range ◽  
Observation Protocol ◽  
Microscopy Techniques ◽  
Scanning Electron

Objectives: the purpose of this study is to use light microscopy and scanning electron microscopy to determine the effect of edema on the structure of the molar vesicle. Methods: samples were taken from the complete hydatidiform mole and processed using conventional light and scanning electron microscopy techniques and an observation protocol that identified four variables: factors underlying the development of edema; the condition of the trophoblast basement membrane, development of the villi, accumulation and degeneration of sulphated mucosubstances at stromal level. Results: light microscopy showed a permeable trophoblastic basement membrane, a swollen syncytium, edematous regions disorganizating the stromal region and causing ischemic necrosis of cells. Using scanning electron microscopy, the basement membrane was found to be distended and thickened, with large irregular holes for the entry and movement of liquid, leaving a wide range of fluids during the influx process and depriving stromal cells of nutrition. Conclusions: a new three-dimensional view of the changes brought about by the entry of fluids into the stroma of molar hydropic vesicles was provided by scanning electron microscopy and confirmed by light microscopy, thereby explaining the changes occurring at the level of the stroma as an effect of the edema.

scholarly journals Glomerulotubular pathology in dogs with subclinical ehrlichiosis

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260702
Author(s):  
Leandro Zuccolotto Crivellenti ◽  
Cristiane Alves Cintra ◽  
Suellen Rodrigues Maia ◽  
Gyl Eanes Barros Silva ◽  
Sofia Borin-Crivellenti ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Interstitial Fibrosis ◽  
Left Kidney ◽  
Control Group ◽  
Tubular Atrophy ◽  
Transmission Electron ◽  
Matrix Expansion

Subclinical stage of ehrlichiosis is characterized by absence of clinical or laboratory alterations; however, it could lead to silent glomerular/tubular changes and contribute significantly to renal failure in humans and animals. The aim of this study was to evaluate glomerular and tubular alterations in dogs with subclinical ehrlichiosis. We evaluated renal biopsies of 14 bitches with subclinical ehrlichiosis and 11 control dogs. Samples were obtained from the left kidney, and the tissue obtained was divided for light microscopy, immunofluorescence, and transmission electron microscopy. Abnormalities were identified by light microscopy in 92.9% of dogs with ehrlichiosis, but not in any of the dogs of the control group. Mesangial cell proliferation and synechiae (46.1%) were the most common findings, but focal segmental glomerulosclerosis and ischemic glomeruli (38.4%), focal glomerular mesangial matrix expansion (30.7%), mild to moderate interstitial fibrosis and tubular atrophy (23%), and glomerular basement membrane spikes (23%) were also frequent in dogs with ehrlichiosis. All animals with ehrlichiosis exhibited positive immunofluorescence staining for immunoglobulins. Transmission electron microscopy from dogs with ehrlichiosis revealed slight changes such as sparse surface projections and basement membrane double contour. The subclinical phase of ehrlichiosis poses a higher risk of development of kidney damage due to the deposition of immune complexes.

Sign in / Sign up

Export Citation Format

Share Document