Trans-dentinal Stimulation of Tertiary Dentinogenesis

2001 ◽  
Vol 15 (1) ◽  
pp. 51-54 ◽  
Author(s):  
A.J. Smith ◽  
P.E. Murray ◽  
A.J. Sloan ◽  
J.B. Matthews ◽  
S. Zhao
Keyword(s):  
Growth Factors ◽  
Tissue Injury ◽  
Careful Consideration ◽  
Active Components ◽  
Restorative Treatment ◽  
Cellular Events ◽  
Dentin Matrix ◽  
Dentin Thickness ◽  
Tertiary Dentinogenesis ◽  
Stimulation Of

Trans-dentinal stimulation of tertiary dentinogenesis has long been recognized, and has traditionally been ascribed to diffusion of irritant substances arising during injury and restorative treatment. Identification of bio-active components, especially growth factors including TGF-βs, sequestered within dentin matrix provides a new explanation for cellular signaling during tertiary dentinogenesis. Both isolated dentin matrix components and pure growth factors (TGF-βs) have been shown to signal cellular events leading to reactionary and reparative tertiary dentinogenesis. Release of these bio-active components from dentin matrix may arise during carious attack and other injury to the tissue, and also during subsequent surgical intervention and restoration of the tooth. Both cavity-conditioning agents and leaching from restorative materials may contribute to release of these components. Distance of diffusion, as determined by cavity residual dentin thickness, and other restorative parameters may influence the signaling process after release of these components. Careful consideration of the interplay between tissue injury and surgical and restorative material factors is required for optimum exploitation of the exquisite regenerative capacity of dentin-pulp for more biological approaches to clinical treatment of dental disease.

Serum stimulation of the c-fos enhancer induces reversible changes in c-fos chromatin structure

1990 ◽  
Vol 10 (3) ◽  
pp. 1126-1133
Author(s):  
J L Feng ◽  
B Villeponteau
Keyword(s):  
Growth Factors ◽  
Dna Sequences ◽  
Chromatin Structure ◽  
Transcriptional Activity ◽  
Dnase I ◽  
Enhancer Activity ◽  
Serum Stimulation ◽  
Fos Protein ◽  
Dnase I Sensitivity ◽  
Stimulation Of

Transcription of the proto-oncogene c-fos is known to be activated by growth factors in serum and subsequently repressed by the Fos protein. We show that generalized DNase I sensitivity of c-fos chromatin correlates closely with enhancer activity during induction, repression, and superinduction of the c-fos gene. Within 90 s of serum stimulation, proximal DNA sequences on both sides of the enhancer exhibit increased DNase I sensitivity. Within 5 min, elevated DNase I sensitivity spreads to chromatin at the distal 3' end of the c-fos gene. These results suggest that an open state of chromatin is propagated in both directions from the enhancer. The induced alterations in chromatin structure precede the increased transcriptional activity of the c-fos gene, suggesting that these changes in chromatin structure potentiate transcription.

Stimulation of Cardiac Angiogenesis through Growth Factors with a Fibrin Gel Delivery Platform

2019 ◽  
Vol 38 (4) ◽  
pp. S246
Author(s):  
L. Melly ◽  
A. Grosso ◽  
C. Stanciu Pop ◽  
M. Nollevaux ◽  
N. Di Maggio ◽  
...  
Keyword(s):  
Growth Factors ◽  
Fibrin Gel ◽  
Delivery Platform ◽  
Stimulation Of

Late signals are required for the stimulation of DNA synthesis in rat mammary fibroblasts by growth factors

1996 ◽  
Vol 16 (3) ◽  
pp. 249-263 ◽  
Author(s):  
Hai-Lan Chen ◽  
Philip S. Rudland ◽  
John A. Smith ◽  
David G. Fernig
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Maximal Response ◽  
High Concentration ◽  
Initial Concentration ◽  
Maximal Stimulation ◽  
Epidermal Growth ◽  
Lag Period ◽  
Stimulation Of

Maximal stimulation of DNA synthesis in quiescent rat mammary (Rama) 27 fibroblasts is elicited by epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) 18 h after the initial addition of the growth factors-the ‘lag’ period. At maximally-stimulating concentrations, EGF and bFGF are interchangeable 9 h after their initial addition. When the initial concentration of growth factor is below that required to elicit a maximal response, it is possible to increase the level of DNA synthesis by increasing the concentration of growth factor 9 h after its initial addition. When the initial concentration of growth factor is high, substitution by a lower concentration of growth factor after 9 h allows a greater proportion of cells to synthesize DNA than would be expected from a continuous low dose of growth factor. Similar results are obtained when both the growth factor and its concentration are changed 9 h after the initial addition of growth factor. However, when EGF at a low concentration is substituted for a high concentration of EGF or bFGF the resulting increase in the levels of DNA synthesis is greater when EGF rather than bFGF is added for a second time. The half-life of the growth-stimulatory signals delivered by EGF and by bFGF 9 h after their initial addition is 1–2 h. These results suggest that to stimulate DNA synthesis: (i) EGF or bFGF must deliver a signal(s) continuously; (ii) the initial signals produced by EGF and bFGF are equivalent; (iii) the signals produced between 9–18 h by EGF may be different to those produced by bFGF.

Potential Role of Growth Factors in Diminishing Radiation Therapy Neural Tissue Injury

2005 ◽  
Vol 32 ◽  
pp. 67-70 ◽  
Author(s):  
Nicolaus H. Andratschke ◽  
Carsten Nieder ◽  
Roger E. Price ◽  
Belinda Rivera ◽  
K. Kian Ang
Keyword(s):  
Radiation Therapy ◽  
Growth Factors ◽  
Potential Role ◽  
Tissue Injury ◽  
Neural Tissue

scholarly journals β-arrestin 2 as an activator of cGAS-STING signaling and target of viral immune evasion

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yihua Zhang ◽  
Manman Li ◽  
Liuyan Li ◽  
Gui Qian ◽  
Yu Wang ◽  
...  
Keyword(s):  
Immune Evasion ◽  
Cyclic Gmp ◽  
Tissue Injury ◽  
Antiviral Drug ◽  
Drug Candidate ◽  
Antiviral Immune Response ◽  
Viral Immune Evasion ◽  
Cellular Events ◽  
Β Blocker

AbstractVirus infection may induce excessive interferon (IFN) responses that can lead to host tissue injury or even death. β-arrestin 2 regulates multiple cellular events through the G protein-coupled receptor (GPCR) signaling pathways. Here we demonstrate that β-arrestin 2 also promotes virus-induced production of IFN-β and clearance of viruses in macrophages. β-arrestin 2 interacts with cyclic GMP-AMP synthase (cGAS) and increases the binding of dsDNA to cGAS to enhance cyclic GMP-AMP (cGAMP) production and the downstream stimulator of interferon genes (STING) and innate immune responses. Mechanistically, deacetylation of β-arrestin 2 at Lys171 facilitates the activation of the cGAS–STING signaling and the production of IFN-β. In vitro, viral infection induces the degradation of β-arrestin 2 to facilitate immune evasion, while a β-blocker, carvedilol, rescues β-arrestin 2 expression to maintain the antiviral immune response. Our results thus identify a viral immune-evasion pathway via the degradation of β-arrestin 2, and also hint that carvedilol, approved for treating heart failure, can potentially be repurposed as an antiviral drug candidate.

Mouse Molar Dentin Size/Shape is Dependent on Growth Hormone Status

2007 ◽  
Vol 86 (5) ◽  
pp. 463-468 ◽  
Author(s):  
J.R. Smid ◽  
J.E. Rowland ◽  
W.G. Young ◽  
K.T. Coschigano ◽  
J.J. Kopchick ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Enamel Organ ◽  
Tooth Crown ◽  
Crown Width ◽  
Morphogenetic Protein ◽  
Root Sheath ◽  
Dentin Matrix ◽  
Dentin Thickness ◽  
Gh Excess ◽  
Gh Receptors

Growth hormone (GH) status affects dental development, but how GH influences tooth size/shape is unclear. Since GH affects dental epithelial proliferation, we hypothesized that GH influences the tooth crown and root dimensions. Dentin matrix dimensions were measured in longitudinal sections of decalcified first mandibular molars from 3 genetically modified mice: giant (GH-Excess) mice and dwarf (GH-Antagonist and GH-Receptor-Knockout) mice. GH status was found to influence crown width, root length, and dentin thickness. Analysis of these data suggests that GH influences both tooth crown and root development prior to dentinogenesis as well as during appositional growth of dentin. This is concordant with the expression of paracrine GH and GH receptors during tooth bud morphogenesis, and of GH receptors in the enamel organ, dental papilla, and Hertwig’s epithelial root sheath during dentinogenesis. Based on prior studies, these GH morphogenetic actions may be mediated by the induction of both bone morphogenetic protein and insulin-like growth factor-1 expression.

scholarly journals Liver Regeneration: Analysis of the Main Relevant Signaling Molecules

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yachao Tao ◽  
Menglan Wang ◽  
Enqiang Chen ◽  
Hong Tang
Keyword(s):  
Growth Factors ◽  
Liver Regeneration ◽  
Normal Liver ◽  
Initial Step ◽  
Signaling Molecules ◽  
Regenerative Process ◽  
Liver Mass ◽  
Termination Phase ◽  
And Function ◽  
Stimulation Of

Liver regeneration is a highly organized tissue regrowth process and is the most important reaction of the liver to injury. The overall process of liver regeneration includes three phases: priming stage, proliferative phase, and termination phase. The initial step aims to induce hepatocytes to be sensitive to growth factors with the aid of some cytokines, including TNF-α and IL-6. The proliferation phase promotes hepatocytes to re-enter G1 with the stimulation of growth factors. While during the termination stage, hepatocytes will discontinue to proliferate to maintain normal liver mass and function. Except for cytokine- and growth factor-mediated pathways involved in regulating liver regeneration, new substances and technologies emerge to influence the regenerative process. Here, we reviewed novel and important signaling molecules involved in the process of liver regeneration to provide a cue for further research.

Sign in / Sign up

Export Citation Format

Share Document