Assessment of oxidative DNA damage, oxidative stress responses and histopathological alterations in gill and liver tissues of Oncorhynchus mykiss treated with linuron

2020 ◽  
pp. 096032712098420
Author(s):  
Ahmet Topal ◽  
Arzu Gergit ◽  
Mustafa Özkaraca
Keyword(s):  
Dna Damage ◽  
Stress Responses ◽  
Oxidative Dna Damage ◽  
Chloride Cells ◽  
Histopathological Changes ◽  
Sod Activity ◽  
Antioxidant Responses ◽  
Secondary Lamellae ◽  
Antioxidant Parameters ◽  
Oxidative Stress Responses

We investigated changes in 8-hydroxy-2-deoxyguanosine (8-OHdG) activity which is a product of oxidative DNA damage, histopathological changes and antioxidant responses in liver and gill tissues of rainbow trout, following a 21-day exposure to three different concentrations of linuron (30 µg/L, 120 µg/L and 240 µg/L). Our results indicated that linuron concentrations caused an increase in LPO levels of liver and gill tissues ( p < 0.05). While linuron induced both increases and decreases in GSH levels and SOD activity, CAT activity was decreased by all concentrations of linuron ( p < 0.05). The immunopositivity of 8-OHdG was detected in the hepatocytes of liver and in the epithelial and chloride cells of the secondary lamellae of the gill tissues. Our results suggested that linuron could cause oxidative DNA damage by causing an increase in 8-OHdG activity in tissues, and it induces histopathological damage and alterations in the antioxidant parameters of the tissues.

scholarly journals Dysregulation of DAF-16/FOXO3A-mediated stress responses accelerates oxidative DNA damage induced aging

Redox Biology ◽  
2018 ◽  
Vol 18 ◽  
pp. 191-199 ◽  
Author(s):  
Aditi U. Gurkar ◽  
Andria R. Robinson ◽  
Yuxiang Cui ◽  
Xuesen Li ◽  
Shailaja K. Allani ◽  
...  
Keyword(s):  
Dna Damage ◽  
Stress Responses ◽  
Oxidative Dna Damage

Evaluation of oxidative stress responses and primary DNA damage in blood and brain of rats exposed to low levels of tembotrione

Chemosphere ◽  
2020 ◽  
Vol 253 ◽  
pp. 126643
Author(s):  
Blanka Tariba Lovaković ◽  
Vilena Kašuba ◽  
Anja Katić ◽  
Nevenka Kopjar ◽  
Ana Marija Marjanović Čermak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Stress Responses ◽  
Low Levels ◽  
Oxidative Stress Responses ◽  
Primary Dna Damage

scholarly journals Efficacy of Melatonin against Oxidative Stress, DNA Damage and Histopathological Changes Induced by Nicotine in Liver and Kidneys of Male Rats

2021 ◽  
Vol 10 (1) ◽  
pp. 31-36
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Histopathological Examination ◽  
Harmful Effect ◽  
Male Rats ◽  
10Mg Dose ◽  
Control Group ◽  
Dna Integrity ◽  
Histopathological Changes ◽  
Antioxidant Parameters

The present study was carried out to investigate and compare the effect of nicotine alone and in combination with melatonin on some oxidants and antioxidant parameters, histopathological changes and DNA integrity in the liver and kidneys of male rats. For this purpose 75 mature male rats weighing 120-140g were randomly divided into five groups; control group (1% ethanol in saline), nicotine group (rats administrated nicotine at a dose of 0.6mg/kg body weight; BW) and nicotine and melatonin groups (rats administrated the same dose of nicotine plus 1, 5 or 10mg/kg BW melatonin, respectively). Nicotine and ‏ melatonin were injected intraperitoneally daily for 21days. Fasting blood samples were collected from each rat one day after the end of last injection (at 22nd day) and sera were collected for determination of total antioxidant capacity (TAC). Five rats were sacrificed from each group; Liver and kidneys were collected for estimation of oxidative stress parameters (MDA, SOD and GSH), histopathological examination and for estimation of DNA damage. The results revealed that nicotine increased MDA, decreased TAC, SOD and GSH, induced histopathological changes and increased the percentage of DNA damage in the liver and kidneys Melatonin administration with nicotine counteracted the effect of nicotine on previous parameters. The effect of melatonin was dose dependent and the 10mg dose produced the highest protective effect. It is concluded that melatonin can ameliorate the harmful effect of nicotine on the liver and kidneys of male rats.

Effect of Wrestling Exercise on Oxidative DNA Damage, Nitric Oxide Level and Paraoxonase Activity in Adolescent Boys

2010 ◽  
Vol 22 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Zuhal Hamurcu ◽  
Nazmi Saritas ◽  
Gulden Baskol ◽  
Nese Akpinar
Keyword(s):  
Nitric Oxide ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Paraoxonase 1 ◽  
Adolescent Boys ◽  
Healthy Life ◽  
Paraoxonase Activity ◽  
Nitric Oxide Level ◽  
Antioxidant Parameters

The objective of the current study is to determine the effects of regular wrestling exercise oxidative DNA damage and antioxidant parameters. The findings of the current study have shown that 8-hydroxy-2’-deoxyguanosine (8-OHdG) obtained from wrestlers in basal status were significantly lower than those of sedentary (p = .001). In contrast, Nitric oxide (NO) and Paraoxonase-1 (PON1) were remarkably higher in wrestlers in basal status than those of sedentary (respectively, p = .001, p = .024). While the NO of wrestlers increased immediately after a 1.5-h exercise compared with those before exercise (p = .002), no differences was found between before and immediately after a 1.5-h exercise in 8-OHdG and PON1 (respectively, p = .777, p = .408).Statistically significant correlations were found between the NO and PON1 in the wrestlers in basal status (r = .671, p = .002). In conclusion, our study suggests that wrestling exercise for a healthy life is important in that it reduces DNA damage as well as enhancing antioxidant parameters.

scholarly journals Effect of Coal Dust Exposure on the SOD Activity and the Oxidative DNA Damage in Asthmatic Mice

2019 ◽  
Vol 11 (2) ◽  
pp. 159-66
Author(s):  
Windy Yuliana Budianto ◽  
Husnul Khotimah ◽  
Eko Suhartono
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Coal Dust ◽  
Negative Control ◽  
Dust Exposure ◽  
Dust Particles ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Sod Activity

BACKGROUND: Coal dust is known to trigger hypersensitivity and inflammation of the respiratory tract as it increases oxidative stress leading to asthma. To date, the relationship of coal dust exposure in the pathomechanism of asthma remains unclear. This study was aimed to examine the effect of coal dust exposure on the superoxide dismutase (SOD) activity and the oxidative DNA damage indicated by increased serum 8-hydroxy-2' -deoxyguanosine (8-OHdG) in asthmatic mice.METHODS: Twenty-four female balb/c mice were divided into four groups. The first group was the control group. The second group was the negative control group which composed of mice exposed to coal dust particles. The third group was composed of ovalbumin (OVA)-sensitized mice. The fourth group was composed of OVA-sensitized mice and exposed to coal dust particles. The inflammatory process was identified by serum interleukin (IL)-13 concentration using Enzyme-linked Immunosorbent Assay (ELISA) method. Meanwhile, the oxidative stress was examined by measuring the SOD activity using the Nitro Blue Tetrazolium (NBT) method, and the 8-OHdG concentration was quantified by ELISA method.RESULTS: There was an increasing IL-13 in OVAtreated coal dust exposed group along with the increment of 8-OHdG (statistically not significant). SOD activity measured in serum was decreased in all groups (p>0.05). Combination of OVA and coal dust showed the worst effect on IL-13, 8-OHdG and SOD activity.CONCLUSION: Coal dust exposure for four weeks does not adequately induce the oxidative DNA damage in asthmatic mice.KEYWORDS: asthma, coal dust, IL-13, SOD, 8-OHdG

scholarly journals Toxic effects of mercuric chloride on DNA damage, hematological parameters and histopathological changes in common (carp Cyprinuscarpio)

2014 ◽  
Vol 38 (2) ◽  
pp. 87-94
Author(s):  
Abdulmotalib J. Alrudainy
Keyword(s):  
Dna Damage ◽  
Common Carp ◽  
Mercuric Chloride ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Inorganic Mercury ◽  
Control Group ◽  
Histopathological Changes ◽  
Biological Organization ◽  
Secondary Lamellae

     The present study aimed to evaluate the effects of mercuric chloride (HgCl2) at different levels of biological organization in common carp Cyprinuscarpio following exposure for 30 days to a range of environmentally levels of mercuric chloride (0.01 and 0.02 mg L-1) and recovery for 3 weeks. Prior to evaluation of genetic damage (determined in erythrocytes using comet assay), enzymatic activity (ALT and AST), hematological parameters and histopathological examination of gill.The maximum tolerated concentration also determined which was found to be 1.63 µg l-1 above which complete mortality over the exposure period was observed. The results indicated that there was a strong induction for DNA damage at high level of Hg. Hematological indices indicated significantly (P≤0.05) increased in Hb, RBCs and Hct value in Hg treatment groups compared to control group after 15 and 30 days exposure. Histopathological examination showed distinct abnormalities in secondary lamellae of gill including epithelial lifting, fusion of the secondary lamellae and necrosis. The present findings suggest that exposure to a low concentration (0.01mg L1) of inorganic mercury can cause significant changes in DNA, hematological parameters and also could cause histopathological changes. Therefore, estimation of these indices could provide a useful indicator for monitoring water bodies pollution.

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