scholarly journals Detection of Caprine Herpesvirus 1–Specific Antibodies in Goat Sera Using an Enzyme-Linked Immunosorbent Assay and Serum Neutralization Test

2010 ◽  
Vol 22 (2) ◽  
pp. 245-248 ◽  
Author(s):  
Mariarosaria Marinaro ◽  
Anna Lucia Bellacicco ◽  
Elvira Tarsitano ◽  
Michele Camero ◽  
Valeriana Colao ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Herpesvirus 1

scholarly journals Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

2002 ◽  
Vol 14 (3) ◽  
pp. 240-242 ◽  
Author(s):  
Juan Francisco Alvarado ◽  
Gaby Dolz ◽  
Marco V. Herrero ◽  
Brian McCluskey ◽  
Mo Salman
Keyword(s):  
New Jersey ◽  
Confidence Interval ◽  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Vesicular Stomatitis

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

scholarly journals The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serum 

2001 ◽  
Vol 46 (No. 2) ◽  
pp. 29-34 ◽  
Author(s):  
K. Kovařčík
Keyword(s):  
Respiratory Syncytial Virus ◽  
Neutralization Test ◽  
Elisa Test ◽  
Bovine Respiratory Syncytial Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Neutralization ◽  
Specificity And Sensitivity ◽  
Reference Serum ◽  
Serum Neutralization Test ◽  
Syncytial Virus

We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation of the newly developed ELISA, field sera collected from 549 head of cattle in the Czech Republic were tested in parallel by a serum neutralization test. The tests showed 98.36% agreement. The specificity and sensitivity of the ELISA relative to serum neutralization test was 97.00% (226/233) and 99.37% (314/316), respectively. Tissue culture-grown viral antigen was used in the tests. The corrected optical density (COD) of each sample tested at dilution 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate, this value was the sample/positive (S/P) ratio. We determined the relationship between the S/P ratio (%) obtained at a dilution 1/100 and the end point titer calculated by serum neutralization test (r = 0.9743). The ELISA test was evaluated by testing acute and convalescent (3 wk later) serum pairs from 9 head of cattle with confirmed BRSV infection for demonstration of seroconversion. The ELISA test demonstrated a clear increase of the S/P ratio (%) between acute and convalescent serum pairs (on average 42.2 ± 13.1).

scholarly journals Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 188 ◽  
Author(s):  
Madoka Tetsuo ◽  
Keita Matsuno ◽  
Tomokazu Tamura ◽  
Takasuke Fukuhara ◽  
Taksoo Kim ◽  
...  
Keyword(s):  
High Throughput ◽  
Neutralization Test ◽  
Classical Swine Fever ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Border Disease Virus ◽  
Diarrhea Virus ◽  
Serum Neutralization ◽  
Border Disease ◽  
Serum Neutralization Test

A serum neutralization test (SNT) is an essential method for the serological diagnosis of pestivirus infections, including classical swine fever, because of the cross reactivity of antibodies against pestiviruses and the non-quantitative properties of antibodies in an enzyme-linked immunosorbent assay. In conventional SNTs, an immunoperoxidase assay or observation of cytopathic effect after incubation for 3 to 7 days is needed to determine the SNT titer, which requires labor-intensive or time-consuming procedures. Therefore, a new SNT, based on the luciferase system and using classical swine fever virus, bovine viral diarrhea virus, and border disease virus possessing the 11-amino-acid subunit derived from NanoLuc luciferase was developed and evaluated; this approach enabled the rapid and easy determination of the SNT titer using a luminometer. In the new method, SNT titers can be determined tentatively at 2 days post-infection (dpi) and are comparable to those obtained by conventional SNTs at 3 or 4 dpi. In conclusion, the luciferase-based SNT can replace conventional SNTs as a high-throughput antibody test for pestivirus infections.

scholarly journals Type-specific seroprevalence of bluetongue in India during 2018 and 2019

2020 ◽  
Vol 13 (10) ◽  
pp. 2092-2096
Author(s):  
G. Naresh ◽  
Kalyani Putty ◽  
Y. Narasimha Reddy ◽  
Y. Krishna Jyothi
Keyword(s):  
Neutralization Test ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sheep Population ◽  
Blood Samples ◽  
Specific Antibodies ◽  
Sheep Industry ◽  
Vaccination Strategies ◽  
Serum Neutralization Test ◽  
Telangana State

Background and Aim: Bluetongue (BT) is a major disease of sheep and goats and is endemic to India. It is known to cause significant economic losses to the sheep industry. The current study aimed to determine the type-specific seroprevalence of BT in sheep population of India during 2018-2019. Materials and Methods: Blood samples (n=405) were collected from 6 months to 1 year old sheep from six districts (Nalgonda, Karimnagar, Khammam, Mahabubnagar, Warangal, and Ranga Reddy) of Telangana state, India. Group- and type-specific seroprevalence (against BT virus [BTV] serotypes BTV-1, 2, 4, 5, 9, 10, 12, 16, 21, 23, and 24) was studied by competitive enzyme-linked immunosorbent assay and serum neutralization test, respectively. Results: Results showed an overall seroprevalence of 14.81% (n=60) with the highest seroprevalence of 50% in Khammam district. Seroprevalence of BTV-1, 2, 4, 5, 9, 10, 12, 16, 21, 23, and 24 was noted as 16.66%, 11.66%, 31.66%, 11.66%, 05%, 6.66%, 16.66%, 8.33%, 13.33%, 6.66%, and 16.66%, respectively. The majority of the sera neutralized more than 1 serotype, indicating superinfection or circulation of multiple serotypes in the sampled flocks. This mixed seroprevalence was observed in 43.33% of the sera with number of BTV serotype-specific antibodies ranging from two to eight in individual animals. Conclusion: Regular monitoring of circulating serotypes, especially in young herds, elucidates pattern of dominating serotypes in a particular area during a season. This knowledge can be applied to design appropriate vaccination strategies by including particular serotypes of virus as part of a multivalent vaccine for a particular period, in a particular area.

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