scholarly journals Preliminary Characterization of a Pleomorphic Gram-Negative Rod Associated with Avian Respiratory Disease

1993 ◽  
Vol 5 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Bruce R. Charlton ◽  
Sally E. Channing-Santiago ◽  
Arthur A. Bickford ◽  
Carol J. Cardona ◽  
Richard P. Chin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Respiratory Disease ◽  
Domestic Fowl ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Preliminary Characterization ◽  
Cellular Fatty Acids

An unidentified, pleomorphic, gram-negative rod (PGNR) bacterium has been isolated from domestic fowl with respiratory disease. The PGNR was isolated in 5% of turkey accessions and 3% of chicken accessions, primarily from the respiratory tract. Preliminary characterization of this organism included reviewing accession records, conducting cultural and biochemical tests, and analyzing cellular fatty acids. The PGNR was also compared with other bacteria capable of inhabiting the avian respiratory system. Biochemical and cellular fatty acid analysis failed to identify the organism, however all 14 isolates were similar.

scholarly journals Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov.

2004 ◽  
Vol 54 (2) ◽  
pp. 583-592 ◽  
Author(s):  
Emenike R. K. Eribe ◽  
Bruce J. Paster ◽  
Dominique A. Caugant ◽  
Floyd E. Dewhirst ◽  
Verlyn K. Stromberg ◽  
...  
Keyword(s):  
16S Rdna ◽  
Type Strain ◽  
Light And Electron Microscopy ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
16S Rdna Sequencing ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Rdna Sequencing ◽  
Sds Page

Sixty strains of Gram-negative, anaerobic, rod-shaped bacteria from human sources initially assigned to Leptotrichia buccalis (n=58) and ‘Leptotrichia pseudobuccalis’ (n=2) have been subjected to polyphasic taxonomy. Full-length 16S rDNA sequencing, DNA–DNA hybridization, RAPD, SDS-PAGE of whole-cell proteins, cellular fatty acid analysis and enzymic/biochemical tests supported the establishment of four novel Leptotrichia species from this collection, Leptotrichia goodfellowii sp. nov. (type strain LB 57T=CCUG 32286T=CIP 107915T), Leptotrichia hofstadii sp. nov. (type strain LB 23T=CCUG 47504T=CIP 107917T), Leptotrichia shahii sp. nov. (type strain LB 37T=CCUG 47503T=CIP 107916T) and Leptotrichia wadei sp. nov. (type strain LB 16T=CCUG 47505T=CIP 107918T). Light and electron microscopy showed that the four novel species were Gram-negative, non-spore-forming and non-motile rods. L. goodfellowii produced arginine dihydrolase, β-galactosidase, N-acetyl-β-glucosaminidase, arginine arylamidase, leucine arylamidase and histidine arylamidase. L. shahii produced α-arabinosidase. L. buccalis and L. goodfellowii fermented mannose and were β-galactosidase-6-phosphate positive. L. goodfellowii, L. hofstadii and L. wadei were β-haemolytic. L. buccalis fermented raffinose. With L. buccalis, L. goodfellowii showed 3·8–5·5 % DNA–DNA relatedness, L. shahii showed 24·5–34·1 % relatedness, L. hofstadii showed 27·3–36·3 % relatedness and L. wadei showed 24·1–35·9 % relatedness. 16S rDNA sequencing demonstrated that L. hofstadii, L. shahii, L. wadei and L. goodfellowii each formed individual clusters with 97, 96, 94 and 92 % similarity, respectively, to L. buccalis.

Leuconostoc carnosum Associated with Spoilage of Refrigerated Whole Cooked Hams in Greece

2006 ◽  
Vol 69 (9) ◽  
pp. 2268-2273 ◽  
Author(s):  
JOHN SAMELIS ◽  
JOHANNA BJÖRKROTH ◽  
ATHANASIA KAKOURI ◽  
JOHN REMENTZIS
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biochemical Characterization ◽  
Eicosenoic Acid ◽  
Cellular Fatty Acid ◽  
Phenotypic Properties ◽  
Cellular Fatty Acids ◽  
Taxonomic Approach ◽  
Leuconostoc Carnosum

A polyphasic taxonomic approach was used to identify a major atypical group of gas-forming, arginine-negative lactic acid bacteria associated with spoilage of whole (nonsliced) refrigerated (4°C) cooked hams produced in two Greek industrial meat plants. Biochemical characterization revealed that the ham isolates shared their phenotypic properties with Leuconostoc carnosum, Weissella viridescens, and Weissella hellenica. However, gas chromatographic analysis of cellular fatty acids clearly differentiated the ham isolates from the Weissella spp. None of the isolates contained eicosenoic acid (n-C20:1), which is typically synthesized by W. viridescens, but all strains contained high amounts of C19cycl acid, which is absent in W. hellenica and has been found in trace amounts in W. viridescens. All strains had similar cellular fatty acid profiles, which were qualitatively similar to those of the cellular fatty acids of L. carnosum. In addition to the phenotypic and chemotaxonomic tests, three representative isolates were studied using a lactic acid bacteria database, which employs 16S and 23S HindIII restriction fragment length polymorphism patterns as operational taxonomic units in a numerical analysis. The isolate patterns were identical to those of the L. carnosum type strain, NCFB 2776T. Based on the polyphasic taxonomic approach, the dominating lactic acid bacteria group was identified as L. carnosum.

scholarly journals Kinneretia asaccharophila gen. nov., sp. nov., isolated from a freshwater lake, a member of the Rubrivivax branch of the family Comamonadaceae

2010 ◽  
Vol 60 (4) ◽  
pp. 809-814 ◽  
Author(s):  
Margarita Gomila ◽  
Jarone Pinhassi ◽  
Enevold Falsen ◽  
Edward R. B. Moore ◽  
Jorge Lalucat
Keyword(s):  
New Genus ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Strictly Aerobic ◽  
Negative Bacterium ◽  
New Genus And Species ◽  
Biochemical Tests ◽  
The Family ◽  
Bacterium Strain ◽  
Physiological And Biochemical

A strictly aerobic, Gram-negative bacterium, strain KIN192T, isolated from fresh water from Lake Kinneret, Israel, was examined using a polyphasic approach to characterize and clarify its phylogenetic and taxonomic position. Sequences of the 16S rRNA and gyrB genes and ITS1 revealed close relationships to species of the genera Pelomonas, Mitsuaria and Roseateles, in the Rubrivivax branch of the family Comamonadaceae of the Betaproteobacteria. Physiological and biochemical tests, cellular fatty acid analysis and DNA–DNA hybridizations indicated that this strain should be assigned to a new genus and species in the Rubrivivax phylogenetic branch, for which the name Kinneretia asaccharophila gen. nov., sp. nov., is proposed. The type strain of Kinneretia asaccharophila is strain KIN192T (=CCUG 53117T =CECT 7319T).

scholarly journals Veillonella denticariosi sp. nov., isolated from human carious dentine

2007 ◽  
Vol 57 (12) ◽  
pp. 2844-2848 ◽  
Author(s):  
Roy Byun ◽  
Jean-Philippe Carlier ◽  
Nicholas A. Jacques ◽  
Helene Marchandin ◽  
Neil Hunter
Keyword(s):  
Fatty Acids ◽  
Dna Hybridization ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Gram Negative ◽  
Cellular Fatty Acids ◽  
Gene Sequence Analysis ◽  
Carious Dentine ◽  
Distinct Lineage

Selective culture of human carious dentine for Veillonella strains resulted in the isolation of two strains of a Gram-negative, coccus-shaped bacterium that has not been described previously. Comparative 16S rRNA and dnaK gene sequence analysis indicated that the two strains were homogeneous and comprised a distinct lineage within the genus Veillonella, phylogenetically most closely related to Veillonella rodentium. This was supported by DNA–DNA hybridization, which showed clearly that the two strains were similar and distinct from other Veillonella species, and the production of major cellular fatty acids (C13 : 0 and C17 : 1 ω8), which is consistent with other members of the genus Veillonella. Based on these observations, strains RBV81 and RBV106T represent a novel species, for which the name Veillonella denticariosi sp. nov. is proposed, with the type strain RBV106T (=CIP 109448T =CCUG 54362T =DSM 19009T).

scholarly journals Atopobium deltae sp. nov., isolated from the blood of a patient with Fournier’s gangrene

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3140-3145 ◽  
Author(s):  
Piet Cools ◽  
Matthijs Oyaert ◽  
Mario Vaneechoutte ◽  
Emmanuel De Laere ◽  
Steven Vervaeke
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Cellular Fatty Acid ◽  
Fatty Acid Analysis ◽  
Fournier’S Gangrene ◽  
Rrna Gene ◽  
Fournier's Gangrene ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type

A Gram-stain-positive, obligately anaerobic, short rod, designated strain HHRM1715T, was isolated from the blood of a patient with Fournier’s gangrene, complicated by sepsis. On the basis of 16S rRNA gene sequence analysis, strain HHRM1715T was shown to belong to the genus Atopobium and was most closely related to Atopobium minutum (95 % similarity). The results of 16S rRNA-gene-based phylogenetic analysis, cellular fatty acid analysis and differential biochemical tests, showed that strain HHRM1715T represented a novel species of the genus Atopobium . We therefore describe Atopobium deltae sp. nov. with HHRM1715T ( = LMG 27987T = CCUG 65171T) as the type strain and propose an emended description of the genus Atopobium with regard to the DNA G+C content.

Isolation, culture, and preliminary characterization of mucin-producing cells from trachea of the domestic fowl

1982 ◽  
Vol 202 (2) ◽  
pp. 285-296 ◽  
Author(s):  
W. H. J. Douglas ◽  
A. W. Gustafson ◽  
J. D. Aghajanian ◽  
E. Y. Gustafson
Keyword(s):  
Domestic Fowl ◽  
Preliminary Characterization

scholarly journals Rheinheimera texasensis sp. nov., a halointolerant freshwater oligotroph

2007 ◽  
Vol 57 (10) ◽  
pp. 2376-2380 ◽  
Author(s):  
Mubina M. Merchant ◽  
Allana K. Welsh ◽  
Robert J. C. McLean
Keyword(s):  
Fatty Acids ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, motile, non-spore-forming bacterium, designated strain A62-14BT, was isolated from a constant-temperature, spring-fed, freshwater lake. On the basis of the complete 16S rRNA gene sequence, strain A62-14BT was shown to belong to the class Gammaproteobacteria, being most closely related to Rheinheimera sp. HTB082 (96.2 % sequence similarity), Rheinheimera baltica (95.01 %), Rheinheimera pacifica (96.35 %), Rheinheimera perlucida and Alishewanella fetalis (95.9 %). The major fatty acids (C16 : 1 ω7c, 38.56 %; C16 : 0, 19.04 %; C12 : 0 3-OH, 12.83 %; C18 : 1 ω7c, 7.70 %) and the motility of strain A62-14BT support its affiliation to the genus Rheinheimera. The salt intolerance of strain A62-14BT, together with the results of other physiological and biochemical tests, allowed the differentiation of this strain from the three species of the genus Rheinheimera with validly published names. Therefore strain A62-14BT represents a novel species of the genus Rheinheimera, for which the name Rheinheimera texasensis sp. nov. is proposed. The type strain is A62-14BT (=ATCC BAA-1235T=DSM 17496T). The description of the genus Rheinheimera is emended to reflect the halointolerance and freshwater origin of strain A62-14BT.

scholarly journals Molecular characterization of Pasteurella multocida pfhaB1 gene fragment from buffalo and cattle isolates from Nusa Tenggara Timur Indonesia

2019 ◽  
Vol 4 (2) ◽  
pp. 70-79
Author(s):  
Ine Karni ◽  
Didik Handijatno ◽  
Lucia Tri Suwanti ◽  
Kusnoto Kusnoto ◽  
Jola Rahmahani ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Gene Fragment ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Phylogenetic Relatedness ◽  
Haemorrhagic Septicaemia ◽  
Strain Vaccine ◽  
Almost All

Almost all regions in Nusa Tenggara Timur East Nusa Tenggara (NTT) Province Indonesia are endemic areas of Haemorragic Septicaemia (HS), which is caused by Pasteurella multocida  (P. multocida ) Serotypes B: 2. The fragment  pfhaB1gene is one of the virulence factors of P. multocida.The objective of this study was to determaine the phylogenetic, homology of P. multocidapfhaB1gene fragment of isolatedfrom Buffalo and Cattle in NTT. The P. multocida isolateswere re-cultured and further microscopic examined the biochemical tests, PCR, sequencing, homology, and phylogenetic relatedness test. P. multocida was observed as gram negative, coccobacilus, no growth on MacConkey Agar, does not produce H2S and gas, nonmotile and indole positive, does not produce urease enzymes, does not use citrate as a carbon source, does not ferment maltose and lactose but it does ferment glucose, sucrose and mannitol. ThepfhaB1gene fragmentfrom buffalo and cattle NTT isolates and also Katha strain vaccine showed DNA band 506 bp. P.multocida isolates from buffalo and cattle in NTT have 91% - 99% score homology with the comparative isolate. The isolate P. multocida from buffalo and cattle in NTT are in one cluster and their phylogenetic relatedness is close to isolates from Iran and India. It is concluded that the  pfhaB1gene fragmentof P. multocida from buffalo and cattle isolates have phylogenetic relatedness close and homolog with the other comparative isolates.    Keywords: Haemorrhagic Septicaemia; Nusa Tenggara Timur; Pasteurella multocida;  pfhaB1gene

Characterization of branched and unsaturated fatty acids in Mycobacterium vaccae strain JOB5

1975 ◽  
Vol 21 (4) ◽  
pp. 510-512 ◽  
Author(s):  
D. H. King ◽  
J. J. Perry
Keyword(s):  
Fatty Acids ◽  
Double Bond ◽  
Unsaturated Fatty Acids ◽  
Mycobacterium Vaccae ◽  
Mass Spectral ◽  
Cellular Fatty Acids ◽  
Branched Chain ◽  
Chain Synthesis ◽  
Chromatographic Mass

After growth of Mycobacterium vaccae strain JOB5 on acetate or propane, the cellular fatty acids were isolated and identified by a combination of gas-chromatographic, mass-spectral, and chemical means. The fatty acids ranged from C12 to C19 and were a mixture of saturated, monounsaturated, and methyl-branched components. The double bond was in the Δ9 position in the C15 to C18 unsaturated acids. The single methyl branch was located on the C10 position of Br-C17, Br-C18, and Br-C19 fatty acids. Branched-chain synthesis occurs at the expense of an unsaturated precursor fatty acid; the double bond serves as the site of methylation. Results suggest that S-adenosylmethionine is the methyl donor involved.

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