Anatomical Study of the Medial Ankle Ligament Complex

2002 ◽  
Vol 23 (6) ◽  
pp. 547-553 ◽  
Author(s):  
Andreas Peter Boss ◽  
Beat Hintermann
Keyword(s):  
Individual Differences ◽  
In Vitro Study ◽  
Anatomical Study ◽  
Deltoid Ligament ◽  
Fibrous Layer ◽  
Ankle Ligament ◽  
Human Cadavers ◽  
Anatomical Relationship ◽  
Insertion Area

The purpose of this in vitro study was to determine insertion area, length and thickness of the various bundles and their anatomical relationship with inter-individual differences. Twelve ankles from human cadavers (ages 56 to 95 years, from nine men and three women) were dissected to the capsuloligamentous structures. Marked inter-individual differences were found for the five main ligaments (tibiospring, tibiocalcaneal, posterior and anterior deep tibiotalar and superficial posterior tibiotalar). The tibionavicular ligament is a thickened fibrous layer of the ankle capsule. The tibiocalcaneal and tibiospring ligaments are the longest, and the tibiocalcaneal and posterior deep tibiotalar ligaments are the thickest of these ligaments. Fibrils run in the direction of the tibia or dorsally. Knowledge of the deltoid ligament complex is necessary for anatomically and biomechanically correct reconstruction that provides stability without hazard to biomechanics of function.

Precision of Implant Placement With Stereolithographic Templates: A Pilot In Vitro Study

2012 ◽  
Vol 38 (5) ◽  
pp. 569-574 ◽  
Author(s):  
Hakan Bilhan ◽  
Selda Arat ◽  
Emre Mumcu ◽  
Onur Geckili ◽  
Olcay Şakar
Keyword(s):  
Vertical Direction ◽  
In Vitro Study ◽  
3 Dimensional ◽  
Implant Placement ◽  
Surgical Guides ◽  
Human Cadavers ◽  
Surgical Templates ◽  
Formalin Fixed ◽  
Stereolithographic Models

This pilot study assesses the accuracy of 2 bone-supported stereolithographic surgical templates with respect to placement of implants in originally planned positions, and it tests the precision of dental volumetric tomography planning. Two mandibles retrieved from formalin-fixed human cadavers were scanned by dental volumetric tomography for planning of the implant positions, leading to stereolithographic models and fabrication of surgical guides. The situation immediately following drilling and implant insertion by an experienced surgeon was scanned and the outcome compared with the initial planning. The 3-dimensional discrepancies were then analyzed and determined. The results show deviations of the placed implants from the original planning, especially in the vertical direction, making the seating of a prefabricated denture impossible. At present, the flapless surgery technique based on stereolithographic surgical templates appears unsafe; further improvement is required.

Tibiotalar Motion — Effect of Fibular Displacement and Deltoid Ligament Transection: In Vitro Study

1999 ◽  
Vol 20 (11) ◽  
pp. 733-737 ◽  
Author(s):  
Marcus Sasse ◽  
Benno M. Nigg ◽  
Darren J. Stefanyshyn
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Deltoid Ligament ◽  
Motion Effect

In vitro study of the interaction between human gut microbiota and herbal extracts using willow bark extract as an example

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
EM Pferschy-Wenzig ◽  
K Koskinen ◽  
C Moissl-Eichinger ◽  
R Bauer
Keyword(s):  
Gut Microbiota ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bark Extract ◽  
Herbal Extracts ◽  
Human Gut ◽  
Human Gut Microbiota ◽  
Willow Bark

In Vitro Effects of Urokinase — Prevention by Different Inhibitors

1990 ◽  
Vol 64 (03) ◽  
pp. 402-406 ◽  
Author(s):  
M D Oethinger ◽  
E Seifried
Keyword(s):  
In Vitro Study ◽  
Thrombin Time ◽  
Plasma Samples ◽  
Temperature Dependent ◽  
Chloromethyl Ketone ◽  
Control Value ◽  
Dose Time ◽  
In Vitro Effects ◽  
Full Protection

SummaryThe present in vitro study investigated dose-, time- and temperature-dependent effects of two-chain urokinase plasminogen activato(u-PA, urokinase) on normal citrated plasma. When 10 μg/ml u-PA wereadded to pooled normal plasma and incubated for 30 min at an ambient temperature (25° C), α2-antiplas-min decreased to 8% of the control value. Incubation on ice yielded a decrease to 45% of control,whereas α2-antiplasmin was fully consumed at 37° C. Fibrinogen and plasminogen fell to 46% and 39%, respectively, after a 30 min incubation at 25° C. Thrombin time prolonged to 190% of control.Various inhibitors were studied with respect to their suitability and efficacy to prevent these in vitro effects. Aprotinin exhibited a good protective effect on fibrinogen at concentrations exceeding 500 KlU/ml plasma. Its use, however, was limited due to interferences with some haemostatic assays. We could demonstrate that L-Glutamyl-L-Glycyl-L-Arginyl chloromethyl ketone (GGACK) and a specific polyclonal anti-u-PA-antibody (anti-u-PA-IgG) effectively inhibited urokinase-induced plasmin generation without interfering with haemostatic assays. The anti-u-PA-antibody afforded full protection ofα2-antiplasmin at therapeutic levels of u-PA.It is concluded that u-PA in plasma samples from patients during thrombolytic therapy may induce in vitro effects which should be prevented by the use of a suitable inhibitor such as GGACK or specific anti-u-PA-antibody.

Effects of Two Different Doses of Hirudin on APTT, Determined with Eight Different Reagents

1995 ◽  
Vol 73 (02) ◽  
pp. 219-222 ◽  
Author(s):  
Manuel Monreal ◽  
Luis Monreal ◽  
Rafael Ruiz de Gopegui ◽  
Yvonne Espada ◽  
Ana Maria Angles ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Anticoagulant Activity ◽  
Subcutaneous Administration ◽  
In Vitro Study ◽  
Ex Vivo Model ◽  
Suitable Candidate ◽  
Significant Prolongation ◽  
High Doses ◽  
Different Doses

SummaryThe APTT has been considered the most suitable candidate to monitor the anticoagulant activity of hirudin. However, its use is hampered by problems of standardization, which make the results heavily dependent on the responsiveness of the reagent used. Our aim was to investigate if this different responsiveness of different reagents when added in vitro is to be confirmed in an ex vivo study.Two different doses of r-hirudin (CGP 39393), 0.3 mg/kg and 1 mg/kg, were administered subcutaneously to 20 New Zealand male rabbits, and the differences in prolongation of APTT 2 and 12 h later were compared, using 8 widely used commercial reagents. All groups exhibited a significant prolongation of APTT 2 h after sc administration of hirudin, both at low and high doses. But this prolongation persisted 12 h later only when the PTTa reagent (Boehringer Mannheim) was used. In general, hirudin prolonged the APTT most with the silica- based reagents.In a further study, we compared the same APTT reagents in an in vitro study in which normal pooled plasma was mixed with increasing amount of hirudin. We failed to confirm a higher sensitivity for silica- containing reagents. Thus, we conclude that subcutaneous administration of hirudin prolongs the APTT most with the silica-based reagents, but this effect is exclusive for the ex vivo model.

Sign in / Sign up

Export Citation Format

Share Document