scholarly journals Plasma Fibrinogen Level Is an Important Determinant of Prolonged Euglobulin Clot Lysis Time in Hemodialysis Patients

2001 ◽  
Vol 7 (4) ◽  
pp. 296-299 ◽  
Author(s):  
Jacek Borawski ◽  
Michal Myśliwiec
Keyword(s):  
Important Determinant ◽  
Chronic Hemodialysis ◽  
Plasma Fibrinogen ◽  
Clot Lysis ◽  
Laboratory Assessment ◽  
Reactive Protein ◽  
Simple Index ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Clot Lysis Time

The euglobulin clot lysis time (ECLT), a traditional measure of plasminogen activation, directly depends on plasma fibrinogen (FBG) level. This fact was neglected in studies concluding that prolonged ECLT in chronic hemodialysis (HD) patients pointed exclusively to impaired fibrinolysis. We studied the relations between ECLT and plasma FBG levels in HD patients in relation to certain hepatic and inflammatory markers. Median ECLT of 320 minutes (range, 150 to 620 minutes) and plasma FBG of 306 mg/dL (range, 171 to 553 mg/dL) were higher in 75 HD patients than in 60 healthy controls (Mann-Whitney p < 0.0001). There were positive associations between these parameters both in the patients (Spear-man p = 0.273, p = 0.018) and the controls (p= 0.672, p < 0.0001). The FBG-corrected ECLT (plasma FBG/ECLT) (in mg/min x dL]) in the patients (0.92 [range, 0.47 to 2.43]) was not different (p = 0.065) from that in the controls (1.08 [0.58 to 1.67]). In the patients, serum alanine aminotransferase inversely correlated with ECLT (p = -0.306, p = 0.008) and FBG (p = -0.310, p = 0.007). whereas serum C-reactive protein was associated positively with these variables (p = 0.383, p = 0.0007: p = 0.477. p <0.0001, respectively). The FBG-corrected ECLT was not related to either marker. In conclusion, increased plasma FBG level, a continuum between liver dysfunction and stimulation by chronic inflammation, is an important determinant of prolonged ECLT in HD patients. The FBG-corrected ECLT value suggests that baseline activation of fibrinolysis is normal in these patients, and that this simple index could be useful in its laboratory assessment.

Relationships between Euglobulin Clot Lysis Time and the Plasma Levels of Tissue Plasminogen Activator and Plasminogen Activator Inhibitor 1

1990 ◽  
Vol 63 (01) ◽  
pp. 082-086 ◽  
Author(s):  
Tetsumei Urano ◽  
Kenji Sakakibara ◽  
Andrzej Rydzewski ◽  
Shoko Urano ◽  
Yumiko Takada ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Slight Modification ◽  
Clot Lysis ◽  
Plasminogen Activator Inhibitor 1 ◽  
Lysis Time ◽  
Tissue Plasminogen ◽  
Euglobulin Clot Lysis Time ◽  
Pai 1 ◽  
Clot Lysis Time

SummaryThe relationships between tissue plasminogen activator (tPA), its fast acting inhibitor (PAI-1) and euglobulin clot lysis time (ELT) were investigated with healthy volunteers’ plasma. Turbidimetric clot lysis assay by the microtiter plate reader was utilized for ELT with a slight modification. Both tPA and PAI-1 showed the significant correlation with ELT. tPA had a significantly positive, not negative, correlation with ELT (R = 0.387, p <0.001). Higher correlation coefficients (R = 0.580, p <0.001 and R = 0.599, p <0.001) were obtained between ELT and total PAI-1 or free PAI-1 than tPA or tPA-PAI-1 complex (R = 0.427, p <0.001). The positive correlation was also obtained between tPA and PAI-1. These data suggest that PAI-1 is a highly important factor for ELT, especially, the amounts of free PAI-1 being the key factor to determine the ELT, which can represent the potential activity of the fibrinolytic system.

Relationship between Plasma Fibrinolytic Activity and Serum Fibrinogen Degradation Products (FDP) Tested by Various Methods

1971 ◽  
Vol 26 (01) ◽  
pp. 083-087 ◽  
Author(s):  
B Lipiński ◽  
A Nowak ◽  
A Odrzywolska ◽  
J Dosiak
Keyword(s):  
Healthy Subjects ◽  
Fibrinolytic Activity ◽  
Degradation Products ◽  
Clot Lysis ◽  
Proteolytic Degradation ◽  
Fibrinogen Degradation Products ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Fibrinogen Content ◽  
Clot Lysis Time

SummaryIt was found in the present work that the level of serum fibrinogen degradation products (FDP) determined by the immunoassay method correlated well with the staphylococcal clumping titer in serum (correlation coefficient r = 0.68). The content of FDP in serum of 30 healthy subjects and patients with various diseases did not correlate, however, neither with blood fibrinolytic activity estimated by the euglobulin clot lysis time, nor with fibrinogen content and plasma anticlotting activity. It is concluded, that FDP appear in circulation as a result of local proteolytic degradation of intravascularly deposited fibrin without generalized activation of fibrinolysis.

Changes in Platelet Function, Blood Coagulation and Fibrinolysis During Insulin-Induced Hypoglycaemia in Juvenile Diabetics and Normal Subjects

1982 ◽  
Vol 47 (03) ◽  
pp. 254-258 ◽  
Author(s):  
J Dalsgaard-Nielsen ◽  
S Madsbad ◽  
J Hilsted
Keyword(s):  
Insulin Infusion ◽  
Adenosine Diphosphate ◽  
Normal Subjects ◽  
Clot Lysis ◽  
Control Group ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Juvenile Diabetics ◽  
Clot Lysis Time

SummaryHaemostatic parameters were assessed before insulin induced hypoglycaemia and 0, 1 and 2 fu after discontinuation of insulin infusion in 7 non-diabetics, aged 28 (22-31) years (mean and range), and 8 juvenile diabetics, aged 3L (27-35) years, with a mean duration of diabetes of 4 years. The patients were normoglycaemic for at least L0 hr before the study.Platelet aggregation in vitro was induced by lower adenosine diphosphate (ADP) concentrations in the diabetics than in the controls before hypoglycaemia and 0 and 60 min after insulin infusion. Platelet counts decreased significantly in the diabetics after hypoglycaemia, whereas no changes were seen in the control group. The activated partial thromboplastin time (APTT) was reduced in both groups and significantly lower in the diabetics than in the controls 120 min after insulin infusion.Fibrinogen and factor VIII R: Ag increased after insulin infusion; highest values were seen in the diabetics. The euglobulin clot lysis time (ELT) was reduced in both groups during insulin infusion; L20 min after end of insulin infusion ELT was significantly longer in the diabetics than in the control group.

scholarly journals Accelerated Hemostasis in Coronary Artery Disease

1977 ◽  
Author(s):  
T. Wajima ◽  
L. L. Burkett
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Antithrombin Iii ◽  
Artery Bypass ◽  
Laboratory Data ◽  
Clot Lysis ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Artery Disease ◽  
Clot Lysis Time

Reduced antithrombin III levels and positive paracoagulation tests occur in some cases of coronary artery disease. This could be related to the cause of atherosclerosis or it could be the result of the disease itself. Thirty-one patients who had arteriosclerotic heart disease, well documented coronary artery occulusions (1-2 vessels), and were subjected to coronary artery bypass surgery were studied for active hemostatic mechanisms of coagulation. Plasma fibrino-peptide A (FPA) levels, fibrinogen, paracoagulation tests, and antithrombin III assays were performed. In addition, PT, PTT, TT, ECLT, and EDP were examined. The blood samples were taken 2-3 days before surgery. Ten of 31 had elevated levels of FPA, and 21 had normal FPA. Eleven patients had positive paracoagulation tests. Six of 31 showed decreased antithrombin III. Seven had an increased fibrinogen level (over 500 mg%). Four of ten patients with elevated FPA had positive tests for paracoagulation, decreased antithrombin III and increased fibrinogen. PT, PTT, TT, Platelet counts, FDP, and ECLT were normal in all patients, except three who had shortened euglobulin clot lysis time. Evidence for activated fibrinolysis was not observed except in 3 cases with shortened englobulin clot lysis time. There was no difference between elevated FPA groups and normal groups in the postoperative period. The degree or extent of coronary artery occulsion was not correlated with the level of FPA or positive paracoagulation tests. Since there were no clinical and laboratory data suggesting disseminated intravascular coagulation, the increased FPA, positive paracoagulation and the reduced level of antithrombin III strongly favor an accelerated hemostatis, probably of localized nature.

scholarly journals Fibrinolytic parameters, lipid status and lipoprotein(a) in ischemic stroke patients

2010 ◽  
Vol 138 (suppl. 1) ◽  
pp. 12-17 ◽  
Author(s):  
Biljana Vuckovic ◽  
Mirjana Djeric ◽  
Tatjana Ilic ◽  
Visnja Canak ◽  
Suncica Kojic-Damjanov ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Plasminogen Activator ◽  
Clot Lysis ◽  
Stroke Patients ◽  
Lipid Status ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Fibrinolytic Parameters ◽  
Pai 1 ◽  
Clot Lysis Time

Introduction. Ischemic stroke is the third leading cause of mortality and morbidity in most countries in the world. Impaired fibrinolysis, as well as disordered lipid metabolism have been recognized as risk factors for this disease. Objective. To study some of fibrinolytic parameters, lipid status and lipoprotein(a) - Lp(a) in ischemic stroke patients in Serbia and to examine associations between Lp(a) and fibrinolytic parameters. Methods. Sixty ischemic stroke patients (case group, mean age 63.48?9.62 years) and 30 age and sex matched healthy controls (control group, mean age 60.2?7.96 years) were studied. Results. A significantly longer euglobulin clot lysis time (219.7?78,8 min. vs 183.5?58,22 min; p=0.005) and higher levels of plasminogen activator inhibitor-1 (PAI-1) (48.5?17.1 ng/ml vs 27.1?10.1 ng/ml; p=6.2?10-11), tissue-type plasminogen activator antigen (t-PA) (11.1?7.14 ng/ml vs 6,.0?3.66 ng/ml; p=5.2?10-5) and D-dimer (382.27?504.22ng/ml vs 116.12?88.81 ng/ml; p=0.0002) were found in cases compared to controls. There were no significant differences in fibrinogen levels (4.30?0.84 g/l vs 4.09?0.64 g/l; p=0.23) or plasminogen activity (92.67?11.37 % vs 96.87?9.48%; p=0.085). There was no significant difference in Lp(a) concentration between cases and controls (0.15?0.11 g/l vs 0.12?0.11 g/l; p=0.261). However, in the cases, but not in the controls, multivariate analysis of associations between fibrinolytic parameters and Lp(a) showed the highest correlation between t-PA and PAI-1, and the latent effect of Lp(a) on t-PA and PAI-1. Conclusions. Our results show that there are important differences in the characteristics of the fibrinolytic mechanism in ischemic stroke patients compared to healthy population. The major differences are prolonged euglobulin clot lysis time and elevated PAI-1 and t-PA antigen in ischemic stroke patients. In addition, Lp(a) appears to be involved in the inhibition of fibrinolysis in ischemic disease through a mechanism unrelated to its serum concentrations.

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