scholarly journals Specificity of Quantitative Latex Agglutination Assay for D-dimer in Exclusion of Pulmonary Embolism in the Emergency Department

2013 ◽  
Vol 20 (8) ◽  
pp. 807-812 ◽  
Author(s):  
Paul D. Stein ◽  
Fadi Matta ◽  
Michel J. Sabra ◽  
Christopher Tana ◽  
Andrew Gough ◽  
...  
Keyword(s):  
Emergency Department ◽  
Pulmonary Embolism ◽  
Latex Agglutination ◽  
Agglutination Assay ◽  
D Dimer ◽  
Latex Agglutination Assay

scholarly journals Screening for Pulmonary Embolism with a D-Dimer Assay: Do we Still Need to Assess Clinical Probability as Well?

2005 ◽  
Vol 98 (2) ◽  
pp. 54-58 ◽  
Author(s):  
Christopher J Hammond ◽  
Tajek B Hassan
Keyword(s):  
Pulmonary Embolism ◽  
Risk Stratification ◽  
Retrospective Analysis ◽  
Emergency Departments ◽  
Latex Agglutination ◽  
Agglutination Assay ◽  
Clinical Risk ◽  
Clinical Probability ◽  
D Dimer ◽  
Latex Agglutination Assay

Clinical risk stratification and D-dimer assay can be of use in excluding pulmonary embolism in patients presenting to emergency departments but many D-dimer assays exist and their accuracy varies. We used clinical risk stratification combined with a quantitative latex-agglutination D-dimer assay to screen patients before arranging further imaging if required. Retrospective analysis of a sequential series of 376 patients revealed that no patient with a D-dimer of <275 ng/mL was diagnosed with pulmonary embolism, irrespective of clinical probability. We conclude that a latex-agglutination assay could be used to exclude pulmonary embolism without the necessity for clinical risk stratification. If these findings are borne out by further work, D-dimer strategies to exclude pulmonary embolism could substantially reduce imaging workload.

Usefulness of a latex agglutination assay for FDP D-dimer to demonstrate the presence of postmortem blood

2005 ◽  
Vol 119 (3) ◽  
pp. 167-171 ◽  
Author(s):  
Koichi Sakurada ◽  
Ikuko Sakai ◽  
Kazumasa Sekiguchi ◽  
Tomoko Shiraishi ◽  
Hiroshi Ikegaya ◽  
...  
Keyword(s):  
Latex Agglutination ◽  
Agglutination Assay ◽  
Postmortem Blood ◽  
D Dimer ◽  
Latex Agglutination Assay

A latex agglutination assay for D dimer: evaluation and application to the diagnosis of thrombotic disease.

1987 ◽  
Vol 33 (10) ◽  
pp. 1837-1840 ◽  
Author(s):  
C J Hillyard ◽  
A S Blake ◽  
K Wilson ◽  
D B Rylatt ◽  
S Miles ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Degradation Products ◽  
Reference Interval ◽  
Latex Agglutination ◽  
Agglutination Assay ◽  
Fibrin Degradation Products ◽  
Pathological Conditions ◽  
D Dimer ◽  
Latex Agglutination Assay ◽  
Apparently Healthy

Abstract Although latex agglutination assays have been used for some years to diagnose thrombotic disorders, only recently has it been possible to measure specifically the products of fibrin breakdown in the presence of fibrinogen degradation products, by using monoclonal antibodies. We have evaluated a preparation of latex particles coupled to the monoclonal antibody DD-3B6/22, which is specific for cross-linked fibrin degradation products (XDP) and allows accurate discrimination between normal and pathological conditions. Of samples from 515 apparently healthy volunteers, 97.7% failed to agglutinate the latex; the normal reference interval for XDP determined by enzyme immunoassay was less than 78-320 micrograms/L. The use of different anticoagulants with or without the addition of a protease inhibitor had no significant effects on the results of the latex assay. The latex preparation provides a useful, rapid diagnostic tool for assaying small numbers of samples or as an emergency test.

Toxin Production by Psychrotrophic Bacillus spp. Present in Milk

1990 ◽  
Vol 53 (9) ◽  
pp. 790-792 ◽  
Author(s):  
M. W. GRIFFITHS
Keyword(s):  
Bacillus Cereus ◽  
Brain Heart Infusion ◽  
Bacterial Count ◽  
Toxin Production ◽  
Latex Agglutination ◽  
Brain Heart Infusion Broth ◽  
Agglutination Assay ◽  
Bacillus Spp ◽  
Latex Agglutination Assay

Using a reversed passive latex agglutination assay, about 85% of psychrotrophic Bacillus spp. tested were shown to produce diarrhoegenic toxin during growth on brain heart infusion broth at 25°C. The majority of these strains were identified as Bacillus cereus or cereus-related strains. However, a number of other species was capable of synthesizing the toxin. Further investigation of four psychrotrophic Bacilli showed that the toxin was produced during growth in milk at temperatures ranging from 6 to 21°C. Toxin production increased with increasing temperatures and was not synthesized in appreciable quantities until the bacterial count exceeded 1 × 107 cfu/ml.

scholarly journals Performance of the Quantitative Latex Immunoturbidimetric D-dimer Assay for the Diagnosis of Acute Pulmonary Embolism at Rafik Hariri University Hospital

2020 ◽  
Vol 7 (3) ◽  
pp. 125-128
Author(s):  
Rida Salman ◽  
Mira Alsheikh ◽  
Rim Ismail
Keyword(s):  
Pulmonary Embolism ◽  
Negative Predictive Value ◽  
Ct Angiography ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Retrospective Chart Review ◽  
Latex Agglutination ◽  
University Hospital ◽  
Acute Pe ◽  
D Dimer

Background and aims: The diagnostic workup for pulmonary embolism (PE) includes D-dimer assay and computed tomographic angiography. Several D-dimer assays have been approved for PE diagnosis with different sensitivity and specificity. We aimed to study the sensitivity and specificity of the quantitative latex agglutination D-dimer assay used in a referral teaching hospital in Lebanon for the diagnosis of acute PE. Methods: Using a retrospective chart review, we studied 300 patients who had D-dimer test at Rafik Hariri University Hospital in the period between January 1, 2012 and December 31, 2013. Accordingly, 93 patients had a CT angiography after being suspected to have acute PE. A statistical table 2*2 was used to compare the results of CT angiography and D-dimer test. Results: Thirteen patients (13.97%) had PE and 60 patients (64.51%) had positive D-dimer test. Quantitative latex agglutination D-dimer assay had a sensitivity of 69%, specificity of 36%, and negative predictive value of 88%. False positive ratio was also 64%. Moreover, the receiver operating characteristic (ROC) curve was obtained with an area under the curve measuring 0.527. Conclusion: Quantitative latex agglutination D-dimer assay has a high negative predictive value; thus, it can exclude a PE diagnosis if it is associated with low clinical pretest probability.

Predictive Value of Combination of D-dimer and Fibrinogen in Diagnosing Acute CVST

2021 ◽  
pp. 097275312110631
Author(s):  
Abhishek Pathak ◽  
Varun Kumar Singh ◽  
Anand Kumar ◽  
Vijaya Nath Mishra ◽  
Deepika Joshi ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Predictive Value ◽  
Sinus Thrombosis ◽  
Care Center ◽  
Tertiary Care ◽  
Cerebral Venous Sinus Thrombosis ◽  
Latex Agglutination ◽  
D Dimer ◽  
Latex Agglutination Assay

Background: Cerebral venous sinus thrombosis (CVST) is an uncommon subtype of stroke, and the role of D-dimer and fibrinogen in early diagnosis of CVST has been studied with varying results. The present study aims to study the role of the combination of D-dimer and fibrinogen in early diagnosis of acute CVST. Methods: Forty consecutive confirmed acute CVST cases admitted at a tertiary care center were recruited for the study. D-dimer and fibrinogen were assessed by a rapid semiquantitative latex agglutination assay. Results: Out of the 40 CVST patients, 21 (52.50%) were females. The mean age of the patients was 37.58 years ± 19.17 years. Common clinical features were headache ( N = 38 [95%]), papilloedema ( N = 15 [37.5%]), and seizures ( N = 12 [30%]). Although the sensitivity (75%) of the combination of D-dimer and fibrinogen assay was lower than that of D-dimer or fibrinogen alone, the specificity and positive predictive value (75% each) was higher. Conclusions: The combination of D-dimer and fibrinogen testing may aid in an early diagnosis of acute CVST and in better management.

A Rapid and Reliable Method for the Detection of Molds in Foods: Using the Latex Agglutination Assay

1989 ◽  
Vol 52 (4) ◽  
pp. 244-247 ◽  
Author(s):  
H. J. KAMPHUIS ◽  
S. NOTERMANS ◽  
G. H. VEENEMAN ◽  
J. H. VAN BOOM ◽  
F. M. ROMBOUTS
Keyword(s):  
Reliable Method ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Penicillium Digitatum ◽  
Agglutination Assay ◽  
Culture Filtrates ◽  
Latex Beads ◽  
Positive Reactivity ◽  
Latex Agglutination Assay

An agglutination test by using latex beads (0.8 μm diameter) coated with IgG from the antibodies against extra-cellular polysaccharide of Penicillium digitatum has been developed. As low as 5 to 10 ng/ml of the purified extra-cellular polysaccharide of the same species can be detected by this preparation. Analysis of culture filtrates from 25 different molds showed that the positive reactivity was obtained only with the species of genera Penicillium and Aspergillus. The application of this test was confirmed in testing the samples of spices and nuts. Further, the reliability could be enhanced by including specific blocker in the assay, the synthetic epitopes consisting of four β (1–5)-linked D-galactofuranosyl residues.

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